FastQCFastQC Report
Thu 26 May 2016
SRR937595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257377
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117980.9383025138840617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91220.7254785159900332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62810.49953196217204543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29190.23214994389113208No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.16319687730887394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16080.12788527227712929No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16000.1272490271414222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.1257379449441178No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14300.11372881800764606No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14150.11253585837819524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143300.028.7643381
GTACATG96650.028.5795751
TACATGG96500.027.676892
GGTATCA100400.026.0441671
ACATGGG99200.025.9165133
GAGTACT86650.024.5858812-13
TATCAAC171200.023.9282992
ATCAACG171850.023.8091893
TCAACGC173250.023.647994
CATGGGA71750.023.5161744
CAACGCA174600.023.4388585
AACGCAG176900.023.202696
AGTACTT89650.023.12734612-13
GTACTTT93200.022.72923914-15
CATGGGG22950.022.159574
ATGGGAG27250.021.803245
CCGGACA2405.8862497E-921.7842034
ATGGGAT26400.021.2449465
ACGCAGA193500.021.0403187
CGCAGAG193700.020.9695428
Produced by FastQC (version 0.11.5)