##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937595_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1257377 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842932549267246 33.0 31.0 34.0 30.0 34.0 2 31.94512862888378 33.0 31.0 34.0 30.0 34.0 3 31.94262739019403 33.0 31.0 34.0 30.0 34.0 4 35.55576648849152 37.0 35.0 37.0 33.0 37.0 5 35.568642499425394 37.0 35.0 37.0 33.0 37.0 6 35.66047096455558 37.0 35.0 37.0 33.0 37.0 7 35.6157198676292 37.0 35.0 37.0 33.0 37.0 8 35.60866390907421 37.0 35.0 37.0 33.0 37.0 9 37.23125681478189 39.0 37.0 39.0 34.0 39.0 10-11 37.124319913597915 39.0 37.0 39.0 34.0 39.0 12-13 37.01017316206675 39.0 37.0 39.0 33.0 39.0 14-15 38.108694130718156 40.0 38.0 41.0 33.0 41.0 16-17 38.063025250183514 40.0 38.0 41.0 33.0 41.0 18-19 38.08795055102806 40.0 38.0 41.0 33.5 41.0 20-21 38.04579772017462 40.0 38.0 41.0 33.0 41.0 22-23 37.96910075498438 40.0 38.0 41.0 33.0 41.0 24-25 37.98873846109798 40.0 38.0 41.0 33.0 41.0 26-27 37.898010700052566 40.0 38.0 41.0 33.0 41.0 28-29 37.78218585197598 40.0 37.5 41.0 33.0 41.0 30-31 37.49920031939506 40.0 37.0 41.0 33.0 41.0 32-33 37.28283999150613 39.5 36.0 41.0 32.5 41.0 34-35 37.2311279751419 40.0 36.0 41.0 32.0 41.0 36-37 37.07107414880342 40.0 36.0 41.0 31.5 41.0 38-39 36.898828275051955 39.5 36.0 41.0 31.0 41.0 40-41 36.651524562641114 39.0 35.0 41.0 30.5 41.0 42-43 36.32500276368981 39.0 35.0 41.0 30.0 41.0 44-45 35.96125028531618 39.0 35.0 41.0 29.5 41.0 46-47 35.45675998527092 39.0 35.0 41.0 26.5 41.0 48-49 34.99843404165974 39.0 35.0 40.0 24.0 41.0 50-51 34.05322071264227 38.0 34.0 39.5 22.5 40.5 52-53 34.11365883104272 38.0 34.0 39.5 23.0 40.5 54-55 34.3969589073126 38.0 34.0 40.0 22.5 41.0 56-57 34.182036891083584 38.0 34.0 40.0 20.5 41.0 58-59 33.95364278175917 38.0 34.0 40.0 19.5 41.0 60-61 33.6380755334319 38.0 34.0 40.0 18.0 41.0 62-63 33.2918273517012 37.5 33.0 40.0 15.5 41.0 64-65 32.91915710244422 37.0 33.0 40.0 11.5 41.0 66-67 32.488657737496396 36.0 32.0 39.0 8.0 41.0 68-69 32.00282651901538 36.0 31.5 39.0 6.5 40.0 70-71 31.551343789491934 35.0 31.0 38.5 2.0 40.0 72-73 31.104150147489577 35.0 31.0 38.0 2.0 40.0 74-75 30.59037106611621 35.0 30.0 37.0 2.0 39.0 76-77 30.121152605781717 35.0 30.0 37.0 2.0 39.0 78-79 29.582698745086 34.0 29.0 36.0 2.0 39.0 80-81 29.03578083581933 34.0 29.0 36.0 2.0 37.5 82-83 28.543637270285682 34.0 28.5 35.0 2.0 37.0 84-85 28.039898137153774 34.0 27.0 35.0 2.0 36.5 86-87 27.54787386758307 33.5 26.0 35.0 2.0 36.0 88-89 27.107585075915974 33.0 26.0 35.0 2.0 36.0 90-91 26.58213686110053 33.0 25.0 35.0 2.0 35.0 92-93 25.987755462363317 32.5 23.5 35.0 2.0 35.0 94-95 25.304960246608616 32.0 19.5 34.5 2.0 35.0 96-97 24.518764062011634 32.0 12.5 34.0 2.0 35.0 98-99 23.543550184232732 31.0 2.0 34.0 2.0 35.0 100-101 20.821731668385855 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08008635994537983 1101 2 0.1986669633995426 1101 3 0.2207715529850489 1101 4 0.008736214984395474 1101 5 0.14724394804318308 1101 6 0.015843209743991338 1101 7 0.15023022574661127 1101 8 0.0814656057929497 1101 9 0.1672736470437357 1101 10-11 0.12071116432966988 1101 12-13 0.18832202129829767 1101 14-15 0.32443101857870005 1101 16-17 0.2785553401266654 1101 18-19 0.3148207370494731 1101 20-21 0.34767182169561295 1101 22-23 0.28117768179979663 1101 24-25 0.24127667244737694 1101 26-27 0.29724925735307295 1101 28-29 0.4903991401856942 1101 30-31 0.3574459772102898 1101 32-33 0.3870763686600682 1101 34-35 0.4053557670831083 1101 36-37 0.43436010660538926 1101 38-39 0.2852075993258367 1101 40-41 0.3181280772109929 1101 42-43 0.35556942536420877 1101 44-45 0.564254460622756 1101 46-47 0.5121425910173159 1101 48-49 0.5717387597821642 1101 50-51 0.31164436691359754 1101 52-53 0.3391397422509286 1101 54-55 0.42362727908213316 1101 56-57 0.25829158724828716 1101 58-59 0.2670916448588798 1101 60-61 0.44464867210458436 1101 62-63 0.6936400498241042 1101 64-65 0.7973949724726879 1101 66-67 0.6414136255364653 1101 68-69 0.6604563022238779 1101 70-71 0.5860433639452971 1101 72-73 0.476980728191279 1101 74-75 0.44625427088579883 1101 76-77 0.3675560549950383 1101 78-79 0.6094120065398236 1101 80-81 0.5742608175896535 1101 82-83 0.7353319176829061 1101 84-85 0.8907609414041389 1101 86-87 0.8767064963515061 1101 88-89 0.757660402526465 1101 90-91 0.8872104617770766 1101 92-93 0.710645610247294 1101 94-95 1.0579987375590356 1101 96-97 1.545163514625969 1101 98-99 1.8118175999139936 1101 100-101 2.0588605654425685 1102 1 0.20218115503720568 1102 2 0.2813898303954083 1102 3 0.23006545901485964 1102 4 0.06093882030273079 1102 5 0.15115020699395387 1102 6 0.10138621353625155 1102 7 0.2262255512968352 1102 8 0.20381016315234035 1102 9 0.23682785576708199 1102 10-11 0.2971842461439991 1102 12-13 0.39481985019968846 1102 14-15 0.4525799708374407 1102 16-17 0.4791413648966696 1102 18-19 0.4412774926605252 1102 20-21 0.4992079169260748 1102 22-23 0.4451721022482289 1102 24-25 0.49467338200135913 1102 26-27 0.34320809240686856 1102 28-29 0.4283884966468605 1102 30-31 0.5038605138325352 1102 32-33 0.6124343994335248 1102 34-35 0.518942891723654 1102 36-37 0.6325006379803355 1102 38-39 0.7129229047117676 1102 40-41 0.6702149669833446 1102 42-43 0.59126308850594 1102 44-45 0.6192872275991448 1102 46-47 0.6453194410339265 1102 48-49 0.741125696528151 1102 50-51 0.4791749857053418 1102 52-53 0.5720702670953983 1102 54-55 0.5103904517503466 1102 56-57 0.5226981727231248 1102 58-59 0.5973269140407353 1102 60-61 0.7272987872472783 1102 62-63 0.7832654637076786 1102 64-65 0.72193039174104 1102 66-67 0.7791363206775515 1102 68-69 0.9526987778491431 1102 70-71 0.9191193924961034 1102 72-73 0.8091539463437272 1102 74-75 0.8655693150524755 1102 76-77 0.922837724965138 1102 78-79 0.845324784514915 1102 80-81 0.8921093677082332 1102 82-83 0.9823946332174316 1102 84-85 1.0428131737976187 1102 86-87 1.1884951608107954 1102 88-89 1.2828336894208192 1102 90-91 1.4550988249176662 1102 92-93 1.288273269699875 1102 94-95 1.4861428205992588 1102 96-97 1.5811255576354917 1102 98-99 1.8176964652142225 1102 100-101 2.075192497204771 1103 1 0.21953234798949595 1103 2 0.31280593548244084 1103 3 0.14659577507095634 1103 4 0.1965475911135286 1103 5 0.133094150715813 1103 6 0.19450905850330003 1103 7 0.3242315969442444 1103 8 0.2782691516619167 1103 9 0.27428960826530613 1103 10-11 0.30608054572709165 1103 12-13 0.4057741195915199 1103 14-15 0.40170732692977396 1103 16-17 0.3469032273997854 1103 18-19 0.2512428734716039 1103 20-21 0.18941817418963325 1103 22-23 0.3511872440990871 1103 24-25 0.33518476612182724 1103 26-27 0.381817269958475 1103 28-29 0.37710333436651666 1103 30-31 0.44854491994473733 1103 32-33 0.3039838970161952 1103 34-35 0.45155911328646425 1103 36-37 0.3949339648427923 1103 38-39 0.38579005761858554 1103 40-41 0.42104578129692527 1103 42-43 0.5086161302800747 1103 44-45 0.5342402675244529 1103 46-47 0.7450564461274283 1103 48-49 0.7222388259364365 1103 50-51 0.6952606601093265 1103 52-53 0.7341333673847359 1103 54-55 0.9639931122049816 1103 56-57 0.9604587410509566 1103 58-59 0.9501468295495812 1103 60-61 0.9910817540516845 1103 62-63 0.9345372220296824 1103 64-65 0.9256717138335375 1103 66-67 1.0576959960244423 1103 68-69 0.9955475349786624 1103 70-71 0.9618537537743812 1103 72-73 1.0735250695954335 1103 74-75 1.0329731994271576 1103 76-77 0.9090432030242432 1103 78-79 1.0564949038563647 1103 80-81 1.158945201573104 1103 82-83 1.1624178821520488 1103 84-85 1.0622234920722988 1103 86-87 1.1393996968951754 1103 88-89 1.3768520574657828 1103 90-91 1.4658393242844454 1103 92-93 1.159345678807174 1103 94-95 1.4111915832752437 1103 96-97 1.6037401152586455 1103 98-99 1.7350575955838927 1103 100-101 1.5978209209917118 1104 1 0.28642017467614167 1104 2 0.2520107106364229 1104 3 0.29298706184178513 1104 4 0.13286896278126648 1104 5 0.2444351414175827 1104 6 0.16233146462919024 1104 7 0.14681132156434984 1104 8 0.23452346190423867 1104 9 0.20772282031733624 1104 10-11 0.12343744568190829 1104 12-13 0.31693913350319747 1104 14-15 0.24012555717399664 1104 16-17 0.2818946663667532 1104 18-19 0.3644744256344623 1104 20-21 0.28059793210264417 1104 22-23 0.29038072344558685 1104 24-25 0.2676007609852036 1104 26-27 0.24126175403732475 1104 28-29 0.2986973511556599 1104 30-31 0.4342283757484111 1104 32-33 0.4191624896774897 1104 34-35 0.3139000802075813 1104 36-37 0.5215112162755773 1104 38-39 0.6670800388318696 1104 40-41 0.6312304380232803 1104 42-43 0.6696444054697182 1104 44-45 0.793301883181627 1104 46-47 0.8196081809256484 1104 48-49 0.8520211141103218 1104 50-51 0.9098435475555249 1104 52-53 0.8705361877695097 1104 54-55 0.8083291565321034 1104 56-57 0.8497045378215446 1104 58-59 0.7596216339537563 1104 60-61 0.9127054966499557 1104 62-63 0.9405394574105301 1104 64-65 1.1643200218404814 1104 66-67 1.244946001969602 1104 68-69 1.2053308932816762 1104 70-71 1.1334335021751265 1104 72-73 1.340239297556721 1104 74-75 1.2124439611212914 1104 76-77 1.2713699015569766 1104 78-79 1.1295496469064688 1104 80-81 1.2234959851454335 1104 82-83 1.116825625130808 1104 84-85 1.031089274098882 1104 86-87 1.1493316842438723 1104 88-89 1.1829809247935508 1104 90-91 1.3779882624785422 1104 92-93 1.5165815352671856 1104 94-95 1.676874920175674 1104 96-97 1.7750951365423226 1104 98-99 1.8315190352642823 1104 100-101 1.5937407564296322 1105 1 0.11699887276803977 1105 2 0.1371373359520227 1105 3 0.16317231382113562 1105 4 0.08173102012482758 1105 5 0.08980620710428155 1105 6 0.13566771500614294 1105 7 0.1161953720370903 1105 8 0.019342023762320082 1105 9 0.07633878167045083 1105 10-11 0.1179336939302047 1105 12-13 0.03828568419767464 1105 14-15 0.13327484082298469 1105 16-17 0.24863031717297446 1105 18-19 0.2502100823536111 1105 20-21 0.30899209959218865 1105 22-23 0.3009698874169473 1105 24-25 0.3678742338241747 1105 26-27 0.3919497230062916 1105 28-29 0.33168586233995967 1105 30-31 0.30434296825909257 1105 32-33 0.23460380092801358 1105 34-35 0.3549931437538021 1105 36-37 0.3115523702422607 1105 38-39 0.42548585478612466 1105 40-41 0.42284502166457116 1105 42-43 0.5031680364794724 1105 44-45 0.49724137029440385 1105 46-47 0.28337317758484204 1105 48-49 0.48970005404090244 1105 50-51 0.5132677231457805 1105 52-53 0.5910365987178849 1105 54-55 0.5275860790905043 1105 56-57 0.6464917493446549 1105 58-59 0.6673572197178004 1105 60-61 0.7736382016363237 1105 62-63 0.7066143387085404 1105 64-65 0.6615474507227646 1105 66-67 0.6986297550221465 1105 68-69 0.37692194347650343 1105 70-71 0.5050041527744078 1105 72-73 0.5727734003588552 1105 74-75 0.6712305695259246 1105 76-77 0.7469010301572077 1105 78-79 0.7082232001612425 1105 80-81 0.6906567753488915 1105 82-83 0.8607113637439952 1105 84-85 0.7083534813824244 1105 86-87 1.0359341759509917 1105 88-89 1.2044365689773002 1105 90-91 0.9132535459146638 1105 92-93 0.9279911177554645 1105 94-95 1.0806730883511229 1105 96-97 1.3628065975672676 1105 98-99 1.3259546760185366 1105 100-101 0.975810103228671 1106 1 -0.042471522168817444 1106 2 0.04455558727451603 1106 3 -0.04402281232125915 1106 4 0.0665570538632565 1106 5 0.05131579170546985 1106 6 0.017398812152833898 1106 7 0.0880509328505994 1106 8 0.154036006520343 1106 9 0.15046049064013545 1106 10-11 -0.04941316526564776 1106 12-13 -0.008183967869811681 1106 14-15 0.139114692989331 1106 16-17 0.09641281242100064 1106 18-19 0.08727814082086383 1106 20-21 0.019692062316536862 1106 22-23 0.15604195367772178 1106 24-25 -0.12149923119883255 1106 26-27 -0.0800393428024222 1106 28-29 0.25862492325789077 1106 30-31 0.33432607525079305 1106 32-33 0.15476483967618293 1106 34-35 0.374909511466214 1106 36-37 0.32524985056679867 1106 38-39 0.2697863150479023 1106 40-41 0.32406189986836864 1106 42-43 0.28467058893635766 1106 44-45 0.05066451768127678 1106 46-47 0.26811924050931424 1106 48-49 0.46526004801335574 1106 50-51 0.47234778120062515 1106 52-53 0.2774839250490331 1106 54-55 0.3111976139220971 1106 56-57 0.336143195560652 1106 58-59 0.5097050041957942 1106 60-61 0.4185681181230052 1106 62-63 0.5825535000856732 1106 64-65 0.6451689653831991 1106 66-67 0.5298566394914204 1106 68-69 0.2697200759927725 1106 70-71 0.36218255774660335 1106 72-73 0.4195374243315939 1106 74-75 0.33899347522909906 1106 76-77 0.4045284613360458 1106 78-79 0.6532528488448293 1106 80-81 0.25234599686937287 1106 82-83 0.3083648482243646 1106 84-85 0.43810996034018856 1106 86-87 0.7142152264370303 1106 88-89 0.7803625781296617 1106 90-91 0.7808664898187736 1106 92-93 0.7266322087424193 1106 94-95 0.9011148180471267 1106 96-97 0.8010291437851151 1106 98-99 1.2008680960464027 1106 100-101 1.334387006071399 1107 1 0.2036696206254156 1107 2 0.18880583499003123 1107 3 0.14237760604271443 1107 4 0.13756608171748752 1107 5 0.07801677155794096 1107 6 0.03764542670749904 1107 7 0.11562442178597365 1107 8 0.12943235134034126 1107 9 0.29150144627153196 1107 10-11 0.350204251887277 1107 12-13 0.30932401407448396 1107 14-15 0.49266803439635254 1107 16-17 0.3642758532665269 1107 18-19 0.39734502400924754 1107 20-21 0.24566503380172833 1107 22-23 0.2912271898331795 1107 24-25 0.27384922195540895 1107 26-27 0.4334804193906905 1107 28-29 0.3630934358638598 1107 30-31 0.4662001807225522 1107 32-33 0.3762266364393696 1107 34-35 0.4699730917810854 1107 36-37 0.5056376629635864 1107 38-39 0.582981294680188 1107 40-41 0.49815360965587985 1107 42-43 0.41327897823504856 1107 44-45 0.3556325899041042 1107 46-47 0.46480948602291505 1107 48-49 0.5380374893873423 1107 50-51 0.35423840103987914 1107 52-53 0.36725189818566406 1107 54-55 0.5693116731536279 1107 56-57 0.529189270645972 1107 58-59 0.5696838107574962 1107 60-61 0.6169070479919938 1107 62-63 0.738446376888497 1107 64-65 0.5373439075829083 1107 66-67 0.6632142856435692 1107 68-69 0.8562512330994565 1107 70-71 0.9273358661248956 1107 72-73 0.8929603022353696 1107 74-75 0.6504764654305752 1107 76-77 0.6359814504365637 1107 78-79 1.1491476523238546 1107 80-81 1.3123041604717045 1107 82-83 1.1412931208215298 1107 84-85 1.3301073730892803 1107 86-87 1.3585535507792095 1107 88-89 1.286862357938098 1107 90-91 1.4076115079845763 1107 92-93 1.2006895135976485 1107 94-95 1.3200939548961834 1107 96-97 1.5637617211274026 1107 98-99 1.5156085452371961 1107 100-101 1.6093649869147342 1108 1 0.01702477740968078 1108 2 -0.051109212588627884 1108 3 -0.06890145139625048 1108 4 -0.0277405951982459 1108 5 -0.08485663475583749 1108 6 0.040101227592806765 1108 7 0.059588956469355026 1108 8 0.07700371932612882 1108 9 0.04998542326413968 1108 10-11 0.1061906807048345 1108 12-13 0.16051613112593088 1108 14-15 0.028201604518550027 1108 16-17 0.028064386677620234 1108 18-19 0.06992268722127193 1108 20-21 0.1159605118413296 1108 22-23 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-0.9818745248045744 2316 50-51 -0.9607767900208373 2316 52-53 -0.8523216306263706 2316 54-55 -0.7485444501692413 2316 56-57 -0.6877039025525136 2316 58-59 -0.6376491895279699 2316 60-61 -0.7915576353406664 2316 62-63 -0.7536094120271244 2316 64-65 -0.615086662132839 2316 66-67 -0.7579303454889228 2316 68-69 -0.9601505245776423 2316 70-71 -1.0796637464520806 2316 72-73 -0.8422849977116158 2316 74-75 -0.7876337258340556 2316 76-77 -1.109966410580526 2316 78-79 -0.894416537554207 2316 80-81 -0.8825471914307457 2316 82-83 -0.9887856891780658 2316 84-85 -1.1872945477132966 2316 86-87 -0.8974025684655196 2316 88-89 -1.0586583182170344 2316 90-91 -0.9963871054488074 2316 92-93 -1.0912199721492648 2316 94-95 -1.2038900791970413 2316 96-97 -1.1574537676373318 2316 98-99 -1.3507972905770025 2316 100-101 -1.2530692531244156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4445.0 3 797.0 4 682.0 5 787.0 6 934.0 7 1047.0 8 1255.0 9 1786.0 10 2220.0 11 3211.0 12 4736.0 13 6605.0 14 7718.0 15 10799.0 16 14712.0 17 20477.0 18 17378.0 19 9978.0 20 8824.0 21 9100.0 22 9831.0 23 10564.0 24 11699.0 25 13367.0 26 15027.0 27 17735.0 28 20820.0 29 25313.0 30 30653.0 31 37776.0 32 48182.0 33 62962.0 34 85849.0 35 122486.0 36 174767.0 37 238482.0 38 184356.0 39 20016.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44861864527774 20.130921017473415 15.084790780812183 20.33566955643666 2 14.71506573198191 21.310429729609055 41.78621318721974 22.1882913511893 3 19.24149923736915 27.264630911385723 30.447269239386355 23.04660061185877 4 11.603131951808766 17.332020380012278 38.74044188586874 32.32440578231021 5 11.976840522228521 39.86294411791207 33.728600966280645 14.431614393578753 6 28.966352474363983 38.24328523508649 17.791119220441885 14.999243070107642 7 26.643623980329256 35.83265209208788 20.403055785291883 17.120668142290977 8 26.253576610580314 35.09058356420449 19.369034500398 19.2868053248172 9 27.07076936315461 17.218863862535922 21.044286525846623 34.66608024846284 10-11 24.278281573276246 28.561200694676025 26.459204118652707 20.701313613395023 12-13 25.95946228043197 26.32398145772613 27.73281819934697 19.983738062494922 14-15 22.691033037059878 26.975704142450287 27.711388142926747 22.621874677563092 16-17 19.98320327424599 30.842629101920988 28.037105783914267 21.137061839918758 18-19 19.8330876005257 28.435502010016016 32.25769955051015 19.47371083894814 20-21 21.35613664619531 27.03617084080743 32.28510753735455 19.322584975642712 22-23 20.734154212139185 27.336252125658113 32.19193442954686 19.737659232655844 24-25 20.294445475760057 27.53766714284235 32.51890826434363 19.64897911705397 26-27 20.01394975141798 27.676155445322507 32.56382648612453 19.74606831713497 28-29 19.683266030718038 28.081405355099648 32.59437239647243 19.640956217709885 30-31 19.888012545399782 27.838850897477375 32.416997486776495 19.856139070346355 32-33 19.67189731800461 28.10092892702255 32.24020224034169 19.98697151463115 34-35 20.280587039114693 27.920573779896984 32.221572364730484 19.57726681625784 36-37 20.245217204957243 27.63363923411547 32.25612813975174 19.865015421175546 38-39 19.9754966879504 28.27252844945256 32.17346210052173 19.57851276207531 40-41 20.031961276825925 28.226579322838376 32.09304141184081 19.648417988494895 42-43 19.78108610039748 28.373478569510763 31.57847143491102 20.26696389518074 44-45 20.060202398295914 28.54992771489432 31.243379508955265 20.146490377854505 46-47 20.440148469037545 28.928475674590352 30.556503926254695 20.074871930117407 48-49 20.41450244647514 29.148350969785774 30.120527045880273 20.316619537858816 50-51 20.421785266798008 29.53241874149667 29.84998276923322 20.195813222472108 52-53 20.50191431640893 29.831297877834416 29.511793527984725 20.154994277771934 54-55 20.16478937648684 29.606159445114557 29.607155319803248 20.621895858595355 56-57 20.485411603325616 29.937140886145425 29.18910070283356 20.388346807695402 58-59 20.610551723039176 29.578759539248335 29.43875762741568 20.371931110296813 60-61 20.470313473281408 29.61933794162582 29.82827233638517 20.0820762487076 62-63 20.386526613634302 29.524851553451114 29.978714828085597 20.109907004828994 64-65 20.226578076794706 29.753268550462693 29.780750422384422 20.23940295035818 66-67 20.123595107335213 29.806725323096934 29.411509336345414 20.65817023322244 68-69 20.225616440102225 29.811825644163175 29.47173998942062 20.49081792631398 70-71 20.304244632025544 30.006448459917383 29.24517968659449 20.444127221462576 72-73 20.351933419336543 29.756340612539873 29.267125996561198 20.624599971562386 74-75 20.029848094943546 30.34333275049757 29.05393806460555 20.57288108995333 76-77 20.250503460494286 29.955023133691068 29.102924213263314 20.691549192551335 78-79 20.243706777537103 29.99639920686955 29.018409453197258 20.74148456239609 80-81 20.141553584444242 30.333411664429583 28.873944023802032 20.651090727324142 82-83 20.22296393066531 30.3268385164415 28.805083800662757 20.645113752230433 84-85 20.15022681381354 30.185991309785052 28.712318837693708 20.9514630387077 86-87 20.161125561089957 30.160006467643264 28.720836365041112 20.958031606225664 88-89 20.228836014132092 29.992540466871986 28.613144151395463 21.165479367600458 90-91 20.060931311476978 29.771147124144058 29.12033157339638 21.04758999098258 92-93 20.25490684931725 29.98803605750658 28.94397895875467 20.813078134421506 94-95 19.928395869121125 29.826796704887666 28.919338412561352 21.325469013429856 96-97 19.884594229791144 29.78560956638002 28.952327013815953 21.377469190012878 98-99 20.03758244060433 30.015529242890658 28.498291544309325 21.448596772195685 100-101 20.147617492692795 29.51286625624199 28.99260120578842 21.3469150452768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2436.0 1 1450.0 2 570.5 3 732.5 4 721.5 5 793.5 6 1381.0 7 2629.0 8 4009.0 9 4566.0 10 4097.5 11 3616.0 12 3739.5 13 3987.0 14 4087.5 15 4180.0 16 4245.5 17 4295.5 18 4483.0 19 4706.0 20 4978.5 21 5574.0 22 6473.5 23 7608.5 24 8790.0 25 10151.0 26 12186.5 27 14584.5 28 16275.0 29 17973.5 30 20782.0 31 23453.0 32 26530.5 33 29882.0 34 32959.0 35 34925.5 36 37932.0 37 41296.0 38 41584.5 39 41353.5 40 41141.0 41 41502.0 42 41689.5 43 42309.5 44 43777.0 45 43940.0 46 43502.0 47 44748.0 48 50061.0 49 48512.0 50 40200.0 51 35202.0 52 31731.5 53 29838.0 54 28354.5 55 25771.5 56 23097.0 57 20674.5 58 18135.0 59 15914.0 60 13343.0 61 10661.5 62 8303.5 63 5786.0 64 3884.0 65 2809.5 66 1980.0 67 1447.5 68 1083.0 69 766.5 70 555.0 71 419.0 72 319.0 73 270.0 74 219.0 75 173.5 76 143.0 77 95.5 78 59.5 79 39.0 80 26.5 81 24.5 82 19.0 83 10.5 84 7.5 85 6.0 86 4.0 87 2.5 88 4.0 89 4.0 90 1.0 91 1.0 92 2.5 93 2.0 94 0.5 95 0.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.289809659314589 2 0.25394133978910066 3 0.2517940124560891 4 0.2550547687765881 5 0.2616558120595494 6 0.1834771910095381 7 0.18411343614524522 8 0.18673794733003707 9 0.18292047651579438 10-11 0.19115189795900514 12-13 0.18284094587383098 14-15 0.18184681284928864 16-17 0.1885273867742133 18-19 0.18192634349125203 20-21 0.1756434227761443 22-23 0.17317797287527925 24-25 0.1779895767140643 26-27 0.1715873600360115 28-29 0.18681747797200046 30-31 0.19206650034158412 32-33 0.1936173478598702 34-35 0.19095307135409664 36-37 0.16880378756729286 38-39 0.16196415235844144 40-41 0.16765059325882373 42-43 0.1770352090105036 44-45 0.18113103707161812 46-47 0.1829602418367761 48-49 0.1833976603675747 50-51 0.18725489650279908 52-53 0.2084498125860422 54-55 0.1748878816774921 56-57 0.1614472031856794 58-59 0.16200391767942313 60-61 0.15580052760627877 62-63 0.16248110153120346 64-65 0.15937940649463128 66-67 0.15806715090223536 68-69 0.16741200133293355 70-71 0.1621232136423682 72-73 0.15886245732186927 74-75 0.1606916620870272 76-77 0.164230775654398 78-79 0.16685528683918982 80-81 0.16081095804997228 82-83 0.16041330484015534 84-85 0.15444850669290117 86-87 0.150829862483567 88-89 0.15401108816210254 90-91 0.161367672543716 92-93 0.15413038412504762 94-95 0.1592601105316862 96-97 0.15751043640849163 98-99 0.1593396411736496 100-101 0.14053064434930812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1257377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.3974175753956 #Duplication Level Percentage of deduplicated Percentage of total 1 75.72452597890114 40.43494134394277 2 13.469760742864038 14.385008780547636 3 4.611730001829906 7.387634179580742 4 2.07664940531233 4.435508618126378 5 1.1168286321076897 2.981788241440609 6 0.6734150464997513 2.1575174663701 7 0.43501832434046717 1.6260198582429763 8 0.29699004236774407 1.268680104643589 9 0.22527348975466288 1.0826120340986705 >10 1.222499857557998 11.965491962518923 >50 0.09625484023635766 3.523605446764385 >100 0.046724537536205354 4.436657917800481 >500 0.002388469347228151 0.8678170258458864 >1k 0.0014927933420175944 1.278489692067803 >5k 2.985586684035189E-4 1.2277932992655634 >10k+ 1.4927933420175944E-4 0.9404340287434343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11798 0.9383025138840617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9122 0.7254785159900332 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6281 0.49953196217204543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2919 0.23214994389113208 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2052 0.16319687730887394 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1608 0.12788527227712929 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1600 0.1272490271414222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1581 0.1257379449441178 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1430 0.11372881800764606 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1415 0.11253585837819524 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.953064196338887E-5 0.0 5 0.0 0.0 0.0 7.953064196338887E-5 0.0 6 0.0 0.0 0.0 7.953064196338887E-5 0.0 7 0.0 0.0 0.0 7.953064196338887E-5 0.0 8 0.0 0.0 0.0 7.953064196338887E-5 0.0 9 0.0 0.0 0.0 7.953064196338887E-5 0.0 10-11 0.0 0.0 0.0 7.953064196338887E-5 0.0 12-13 0.0 0.0 0.0 7.953064196338887E-5 0.0 14-15 0.0 0.0 0.0 7.953064196338887E-5 0.0 16-17 0.0 0.0 0.0 1.1929596294508329E-4 0.0 18-19 0.0 0.0 0.0 1.5906128392677773E-4 0.0 20-21 0.0 0.0 0.0 1.9882660490847215E-4 0.0 22-23 0.0 0.0 0.0 2.3859192589016659E-4 0.0 24-25 0.0 0.0 0.0 3.1812256785355547E-4 0.0 26-27 0.0 0.0 0.0 5.169491727620276E-4 0.0 28-29 0.0 0.0 0.0 0.001391786234359305 0.0 30-31 0.0 0.0 0.0 0.003578878888352499 0.0 32-33 0.0 0.0 0.0 0.007117992455723303 0.0 34-35 0.0 0.0 0.0 0.01021968749229547 0.0 36-37 0.0 0.0 0.0 0.014116688948501523 0.0 38-39 0.0 0.0 0.0 0.019485007281030274 0.0 40-41 0.0 0.0 0.0 0.024893090934540715 0.0 42-43 0.0 0.0 0.0 0.036623860624140574 0.0 44-45 0.0 0.0 0.0 0.0500247737949716 0.0 46-47 7.953064196338887E-5 0.0 0.0 0.06243155394126026 0.0 48-49 7.953064196338887E-5 0.0 0.0 0.08104172416069325 0.0 50-51 7.953064196338887E-5 0.0 0.0 0.10267405877473502 0.0 52-53 7.953064196338887E-5 0.0 0.0 0.14685333038539755 0.0 54-55 7.953064196338887E-5 0.0 0.0 0.1885273867742133 0.0 56-57 7.953064196338887E-5 0.0 0.0 0.2619341693064212 0.0 58-59 7.953064196338887E-5 0.0 0.0 0.34305542410907786 0.0 60-61 7.953064196338887E-5 0.0 0.0 0.4313344366884395 0.0 62-63 7.953064196338887E-5 0.0 0.0 0.5493976746830902 0.0 64-65 7.953064196338887E-5 0.0 0.0 0.6200208847465796 0.0 66-67 7.953064196338887E-5 0.0 0.0 0.7646871224779839 0.0 68-69 7.953064196338887E-5 0.0 0.0 0.9471701804629797 0.0 70-71 1.1929596294508329E-4 0.0 0.0 1.0948983479099745 0.0 72-73 1.5906128392677773E-4 0.0 0.0 1.321282320258761 0.0 74-75 1.5906128392677773E-4 0.0 0.0 1.5522790698414237 0.0 76-77 1.5906128392677773E-4 0.0 0.0 1.7273657781238243 0.0 78-79 1.5906128392677773E-4 0.0 0.0 1.9194720437863904 0.0 80-81 2.3859192589016659E-4 0.0 0.0 2.237793438244854 0.0 82-83 3.1812256785355547E-4 0.0 0.0 2.51110048935204 0.0 84-85 3.1812256785355547E-4 0.0 0.0 2.8660059791136625 0.0 86-87 3.1812256785355547E-4 0.0 0.0 3.330663754784762 0.0 88-89 3.1812256785355547E-4 0.0 0.0 3.9108000225867023 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14330 0.0 28.764338 1 GTACATG 9665 0.0 28.579575 1 TACATGG 9650 0.0 27.67689 2 GGTATCA 10040 0.0 26.044167 1 ACATGGG 9920 0.0 25.916513 3 GAGTACT 8665 0.0 24.58588 12-13 TATCAAC 17120 0.0 23.928299 2 ATCAACG 17185 0.0 23.809189 3 TCAACGC 17325 0.0 23.64799 4 CATGGGA 7175 0.0 23.516174 4 CAACGCA 17460 0.0 23.438858 5 AACGCAG 17690 0.0 23.20269 6 AGTACTT 8965 0.0 23.127346 12-13 GTACTTT 9320 0.0 22.729239 14-15 CATGGGG 2295 0.0 22.15957 4 ATGGGAG 2725 0.0 21.80324 5 CCGGACA 240 5.8862497E-9 21.784203 4 ATGGGAT 2640 0.0 21.244946 5 ACGCAGA 19350 0.0 21.040318 7 CGCAGAG 19370 0.0 20.969542 8 >>END_MODULE