FastQCFastQC Report
Thu 26 May 2016
SRR937595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257377
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195701.55641466322352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161251.2824316016596453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116810.9289974287743453No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.28957106738869887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.2522711963078695No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27820.22125424594214785No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17040.1355202139056146No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15900.1264537207217883No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.12017080000668058No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.11094524553892746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.10808214242824547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG756.9118105E-638.0087974
GGTATCA105400.034.9852031
GTATCAA153750.030.0719091
TAACTCG856.3988473E-427.9476474
CGAAGTA700.00839052927.1437427
ATGGGCG1501.0581061E-625.3391975
GTACATG85050.024.6951261
TACATGG85350.024.3260772
TATCAAC191550.024.108952
ATCAACG192700.023.9650693
GAGTACT127950.023.96424512-13
TCAACGC194000.023.7799874
CAACGCA195250.023.652085
AACGCAG198700.023.2349436
ACATGGG85600.022.978453
TTACGAA1050.00219338322.6242834
GTACTTT135450.022.49525514-15
AGTACTT130800.022.3889212-13
CATGGGA63850.021.8022294
ATGGGAT24850.021.0310275
Produced by FastQC (version 0.11.5)