##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937595_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1257377 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1541041390132 33.0 31.0 34.0 31.0 34.0 2 32.420813328063105 34.0 31.0 34.0 31.0 34.0 3 32.47564254793908 34.0 31.0 34.0 31.0 34.0 4 36.133959822710295 37.0 35.0 37.0 35.0 37.0 5 36.07195534831638 37.0 35.0 37.0 35.0 37.0 6 36.13288059189885 37.0 35.0 37.0 35.0 37.0 7 36.07596289736491 37.0 35.0 37.0 35.0 37.0 8 36.06308688643104 37.0 35.0 37.0 35.0 37.0 9 37.738278177507624 39.0 37.0 39.0 35.0 39.0 10-11 37.66927699488697 39.0 37.0 39.0 35.0 39.0 12-13 37.55352611030741 39.0 37.0 39.0 35.0 39.0 14-15 38.675576219383686 40.0 38.0 41.0 34.5 41.0 16-17 38.65939531262302 40.0 38.0 41.0 34.5 41.0 18-19 38.75789639861394 40.0 38.0 41.0 35.0 41.0 20-21 38.7376494877829 40.0 38.0 41.0 35.0 41.0 22-23 38.68230172812132 40.0 38.0 41.0 34.5 41.0 24-25 38.68273715838607 40.0 38.0 41.0 34.0 41.0 26-27 38.530122230643634 40.0 38.0 41.0 34.0 41.0 28-29 38.3684340496128 40.0 38.0 41.0 34.0 41.0 30-31 38.25496688741722 40.0 37.5 41.0 34.0 41.0 32-33 38.03156054230354 40.0 37.0 41.0 34.0 41.0 34-35 37.8306291589555 40.0 36.0 41.0 33.0 41.0 36-37 37.63230757362351 40.0 36.0 41.0 33.0 41.0 38-39 37.458790800213464 40.0 36.0 41.0 33.0 41.0 40-41 37.22372605829437 40.0 36.0 41.0 33.0 41.0 42-43 36.902122434242074 40.0 35.0 41.0 32.0 41.0 44-45 36.41151500305795 39.5 35.0 41.0 30.5 41.0 46-47 35.753076046404544 39.0 35.0 41.0 28.5 41.0 48-49 35.17079086065675 39.0 35.0 41.0 25.0 41.0 50-51 34.813587332995596 39.0 35.0 40.5 23.5 41.0 52-53 34.56702723208711 39.0 35.0 40.0 22.5 41.0 54-55 34.33953897677466 38.5 34.5 40.0 20.5 41.0 56-57 34.08004520521689 38.0 34.0 40.0 19.5 41.0 58-59 33.83700075633641 38.0 34.0 40.0 18.0 41.0 60-61 33.50637199503411 38.0 34.0 40.0 15.0 41.0 62-63 33.15690600352957 37.0 33.0 40.0 11.5 41.0 64-65 32.76774587096789 37.0 33.0 39.5 8.0 41.0 66-67 32.35246787558545 36.0 32.0 39.0 6.5 40.5 68-69 31.91712509454205 36.0 32.0 39.0 2.0 40.0 70-71 31.514999479074294 35.0 31.0 38.5 2.0 40.0 72-73 31.033869714492948 35.0 31.0 37.5 2.0 40.0 74-75 30.568658803206993 35.0 30.5 37.0 2.0 39.0 76-77 29.34118446575689 33.5 28.5 35.5 2.0 38.5 78-79 29.917619775135066 34.0 30.0 36.0 2.0 39.0 80-81 29.71008416727839 34.0 30.0 36.0 2.0 37.5 82-83 29.33772726875074 34.0 30.0 35.0 2.0 37.0 84-85 28.958170461206144 34.0 29.0 35.0 2.0 37.0 86-87 28.604623354809256 34.0 29.0 35.0 2.0 36.0 88-89 28.174568566150008 34.0 29.0 35.0 2.0 36.0 90-91 27.629639320585632 34.0 27.0 35.0 2.0 35.5 92-93 27.211313313349933 33.0 26.0 35.0 2.0 35.0 94-95 26.752913803894934 33.0 25.5 35.0 2.0 35.0 96-97 26.208353182856058 33.0 25.0 35.0 2.0 35.0 98-99 25.367468547619367 32.5 21.0 34.5 2.0 35.0 100-101 22.723820699758306 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31421471804873846 1101 2 0.21298844087829139 1101 3 0.25075896390448094 1101 4 0.17281512752399664 1101 5 0.12685849179395348 1101 6 0.19015345925061666 1101 7 0.141042775957537 1101 8 0.08958233385117609 1101 9 0.14338305276233854 1101 10-11 0.3368131849860845 1101 12-13 0.2692742007535145 1101 14-15 0.2896977322767569 1101 16-17 0.39843813362414693 1101 18-19 0.24428983990069497 1101 20-21 0.25811128044954046 1101 22-23 0.1991407865753132 1101 24-25 0.28023869751291386 1101 26-27 0.09804320119801702 1101 28-29 0.04243038346393746 1101 30-31 0.10763175064609953 1101 32-33 0.22517607643699478 1101 34-35 0.15402778061476852 1101 36-37 0.08248736186818206 1101 38-39 -0.05311707068276661 1101 40-41 0.008768864627832329 1101 42-43 0.20749394250070452 1101 44-45 -0.07811791618296127 1101 46-47 -0.21756357305721252 1101 48-49 -0.10769267975715735 1101 50-51 -0.1295499309907484 1101 52-53 -0.12419869532755712 1101 54-55 -0.17359751543951063 1101 56-57 0.03081931087748302 1101 58-59 -0.16842113668032255 1101 60-61 -0.2211913490474089 1101 62-63 0.07494182012368356 1101 64-65 0.08793249919840207 1101 66-67 -0.11715786919123872 1101 68-69 0.11306373465841801 1101 70-71 -0.032993557145733377 1101 72-73 -0.12663891112686798 1101 74-75 -0.10285488426153222 1101 76-77 -0.1069323090063996 1101 78-79 0.03963633715310877 1101 80-81 0.07915378150957153 1101 82-83 0.03727811507157952 1101 84-85 0.09805272855531655 1101 86-87 0.04133400561071099 1101 88-89 0.12348877962659799 1101 90-91 -0.1924147306841597 1101 92-93 -0.11918611079009622 1101 94-95 -0.23910663788110043 1101 96-97 -0.22133897787470502 1101 98-99 -0.2093218364000009 1101 100-101 -0.23482784389854316 1102 1 0.2814173806519946 1102 2 0.21392218513548045 1102 3 0.19590807656101106 1102 4 0.08975771657340914 1102 5 0.015653560707711733 1102 6 0.08055479742643712 1102 7 0.1834725732834741 1102 8 0.14921708517732668 1102 9 0.25274913779608 1102 10-11 0.2286715651975726 1102 12-13 0.30257936899933924 1102 14-15 0.3002413089182525 1102 16-17 0.3170376890931337 1102 18-19 0.24132448582258803 1102 20-21 0.3714108347728384 1102 22-23 0.26021338948768147 1102 24-25 0.2760583302019839 1102 26-27 0.21357600827385426 1102 28-29 0.23176135283942045 1102 30-31 0.24803767161582613 1102 32-33 0.26935220466356924 1102 34-35 0.111944270652927 1102 36-37 0.22594399145241084 1102 38-39 0.2508367347547207 1102 40-41 0.26794390444086247 1102 42-43 0.24537846755140436 1102 44-45 0.2557386194539859 1102 46-47 0.25047201233741845 1102 48-49 0.2428352008152359 1102 50-51 0.3311964342808409 1102 52-53 0.5245086703679291 1102 54-55 0.3112368738459139 1102 56-57 0.3554982288798456 1102 58-59 0.3319139801236375 1102 60-61 0.27263276332269726 1102 62-63 0.30354905177246394 1102 64-65 0.4453866999018601 1102 66-67 0.424239080959417 1102 68-69 0.4730336027885187 1102 70-71 0.4340349592893098 1102 72-73 0.2185451453331524 1102 74-75 0.19512524932691022 1102 76-77 0.2721449454417737 1102 78-79 0.3659799550397551 1102 80-81 0.47264334992495804 1102 82-83 0.6379131184513902 1102 84-85 0.7016502217670535 1102 86-87 0.42287050183563224 1102 88-89 0.4276170176569103 1102 90-91 0.5044408998509944 1102 92-93 0.24999460023037656 1102 94-95 0.19302562155086278 1102 96-97 0.42322611498415696 1102 98-99 0.5027564033488616 1102 100-101 0.5008185591810985 1103 1 0.3112804753948737 1103 2 0.2439817171986718 1103 3 0.26935252408682686 1103 4 0.16527053353080134 1103 5 0.21179275850391832 1103 6 0.12120807862299188 1103 7 0.08671448237690527 1103 8 0.05654489053335965 1103 9 0.32848150234676865 1103 10-11 0.32859923004315306 1103 12-13 0.29485244922896214 1103 14-15 0.32578278261414795 1103 16-17 0.22729703257650158 1103 18-19 0.2896885095984345 1103 20-21 0.24817761514204761 1103 22-23 0.188221422618561 1103 24-25 0.16022733100621878 1103 26-27 0.14072202822264046 1103 28-29 0.06222554578246786 1103 30-31 0.21065680507302886 1103 32-33 0.2894130771225889 1103 34-35 0.35809987674294064 1103 36-37 0.5180046882733649 1103 38-39 0.5098196978614808 1103 40-41 0.40348103340541996 1103 42-43 0.4851602603539362 1103 44-45 0.4645711346836592 1103 46-47 0.2759776651101902 1103 48-49 0.25009067101684934 1103 50-51 0.33635926926424986 1103 52-53 0.3532592365696203 1103 54-55 0.09636333325966007 1103 56-57 0.1713833061143717 1103 58-59 0.30654698898873534 1103 60-61 0.33157089249988303 1103 62-63 0.24443297349334614 1103 64-65 0.36093945141096384 1103 66-67 0.25535382808681817 1103 68-69 0.25608475968878963 1103 70-71 0.04718632233320719 1103 72-73 0.07061628799748831 1103 74-75 0.1782397283751358 1103 76-77 0.1620464494583409 1103 78-79 0.39125681611004026 1103 80-81 0.5434948248225808 1103 82-83 0.6028571453702369 1103 84-85 0.43004673858671083 1103 86-87 0.4005090017015931 1103 88-89 0.40869548165572667 1103 90-91 0.34955353932149436 1103 92-93 0.32909297604039267 1103 94-95 0.22988535143798927 1103 96-97 0.2151932151995446 1103 98-99 0.34423726342078 1103 100-101 0.31178114827119785 1104 1 0.2782336988616123 1104 2 0.20515148723981014 1104 3 0.12195174883772353 1104 4 0.1250919408695097 1104 5 0.1315889583564811 1104 6 0.06745311441286361 1104 7 0.20588406217286348 1104 8 0.17267054747374289 1104 9 0.15622162743296997 1104 10-11 0.16090761747745574 1104 12-13 0.16388647933526101 1104 14-15 0.24573534022275823 1104 16-17 0.2168751638932349 1104 18-19 0.2422163924073999 1104 20-21 0.13623913567663237 1104 22-23 0.23011281640230408 1104 24-25 0.2545217592005571 1104 26-27 0.19783541434900798 1104 28-29 0.16493178344624937 1104 30-31 0.22544836769936438 1104 32-33 0.29702169522917643 1104 34-35 0.31940400744137776 1104 36-37 0.2576737805660585 1104 38-39 0.29239561757801624 1104 40-41 0.23613483474189678 1104 42-43 0.5491765738780003 1104 44-45 0.5310291261701892 1104 46-47 0.26976437648446705 1104 48-49 0.3061583031777957 1104 50-51 0.43520293528884224 1104 52-53 0.6845563519771005 1104 54-55 0.5805700108654221 1104 56-57 0.8173382180070732 1104 58-59 0.7484847550347311 1104 60-61 0.8180729970871639 1104 62-63 0.7301188073617482 1104 64-65 0.6485075235138851 1104 66-67 0.7191311461054113 1104 68-69 0.8023708170952659 1104 70-71 0.7593198031065533 1104 72-73 0.8304995250191993 1104 74-75 0.832233093574537 1104 76-77 0.6338705440101933 1104 78-79 0.6517660786721642 1104 80-81 0.6034280012036426 1104 82-83 0.572354303582884 1104 84-85 0.5798220114696377 1104 86-87 0.6030820677404485 1104 88-89 0.3744893985661477 1104 90-91 0.5228657078787187 1104 92-93 0.5019550958472934 1104 94-95 0.5871747143544503 1104 96-97 0.7612349264549287 1104 98-99 0.8717990336521666 1104 100-101 0.7189064247212258 1105 1 0.2800572453165202 1105 2 0.12040210661883322 1105 3 0.1677716056266476 1105 4 0.0800224344214655 1105 5 0.09955213265180873 1105 6 0.11909483838564228 1105 7 0.05491995954811557 1105 8 0.019539357379073863 1105 9 0.24447914719692676 1105 10-11 0.24772179194425803 1105 12-13 0.12846509464618805 1105 14-15 -0.04395251640060138 1105 16-17 0.05033020495850593 1105 18-19 0.1934953583548591 1105 20-21 -0.022750581744617193 1105 22-23 -0.04877005444164695 1105 24-25 0.02135704281406703 1105 26-27 -0.06967749061252704 1105 28-29 -0.03688318926559475 1105 30-31 -0.10237904580930746 1105 32-33 -0.04445836933997782 1105 34-35 0.019115752286779752 1105 36-37 -0.01991268119800793 1105 38-39 0.04221024734014378 1105 40-41 0.13993862019727743 1105 42-43 0.0509270956937371 1105 44-45 -0.03574407171772265 1105 46-47 -0.20563991984773367 1105 48-49 -0.37185891526343795 1105 50-51 -0.23725997588127257 1105 52-53 0.017919742685528206 1105 54-55 -0.1643714807293577 1105 56-57 -0.05541147008606373 1105 58-59 -0.059982555352505074 1105 60-61 -0.00560886382898218 1105 62-63 -0.25109109560713705 1105 64-65 -0.19858509064993513 1105 66-67 0.17227386640626463 1105 68-69 0.007230059885934281 1105 70-71 -0.10997377959458632 1105 72-73 -0.05237634492007359 1105 74-75 -0.08572919378185162 1105 76-77 -0.15791640143123686 1105 78-79 -5.423277350793398E-4 1105 80-81 -0.13774252167642587 1105 82-83 -0.1022674169603981 1105 84-85 0.013313712677650358 1105 86-87 -0.042376804901028464 1105 88-89 0.06538171826624506 1105 90-91 0.004018551109307822 1105 92-93 0.19856344135139992 1105 94-95 0.0799372926268127 1105 96-97 -0.02628022541858499 1105 98-99 -0.27674106485718397 1105 100-101 -0.47358681976053774 1106 1 0.11840252360173054 1106 2 0.05171246475590152 1106 3 0.09209608356924548 1106 4 0.007322355375933398 1106 5 -0.026737644566686924 1106 6 -0.046325120743325954 1106 7 -0.06793768182838278 1106 8 -0.11548045687714392 1106 9 -0.11763398572910688 1106 10-11 -0.02028783363370934 1106 12-13 -0.45096905566721546 1106 14-15 -0.05454798833415708 1106 16-17 -0.027031643808093975 1106 18-19 0.035819680321324654 1106 20-21 0.04108959030499193 1106 22-23 0.10869578281972991 1106 24-25 -0.02065605389378078 1106 26-27 0.047729247556418386 1106 28-29 -0.07839729453097277 1106 30-31 0.051585589907112706 1106 32-33 -0.04894136133232507 1106 34-35 -0.4549922096338861 1106 36-37 -0.09742092281825876 1106 38-39 -0.06616937213096463 1106 40-41 -0.34077486486470576 1106 42-43 -0.3855562505494845 1106 44-45 -0.2184400398668629 1106 46-47 -0.1819636020476878 1106 48-49 -0.22389341242546124 1106 50-51 -0.2735561507648825 1106 52-53 -0.04966517838790452 1106 54-55 -0.1943889553435767 1106 56-57 -0.18469783962260067 1106 58-59 -0.25912343088091205 1106 60-61 -0.18738384578598044 1106 62-63 -0.3203749007902985 1106 64-65 -0.424010257795544 1106 66-67 -0.42203551621905433 1106 68-69 -0.051643329792675274 1106 70-71 -0.1492308573420793 1106 72-73 0.06168781150019598 1106 74-75 0.021279025367256565 1106 76-77 0.01200604670669847 1106 78-79 -0.17913301728177444 1106 80-81 -0.3514380853450909 1106 82-83 -0.4333604659765591 1106 84-85 -0.589119316722531 1106 86-87 -0.19910770377297737 1106 88-89 -0.35752542570501333 1106 90-91 -0.42146120768285655 1106 92-93 -0.5265230064375714 1106 94-95 -0.3948355506066008 1106 96-97 -0.4376434832577054 1106 98-99 -0.3742539064515249 1106 100-101 -0.572979305940791 1107 1 0.17402291334080644 1107 2 0.06538911344025422 1107 3 0.02363852549372325 1107 4 -0.0033431736343061402 1107 5 -0.02533667572379983 1107 6 0.038033020839854714 1107 7 0.08153661080620367 1107 8 0.07740460312506059 1107 9 0.04445728367527835 1107 10-11 0.08339899891060298 1107 12-13 0.06138093882799467 1107 14-15 0.11684775095903177 1107 16-17 0.172046304006507 1107 18-19 0.26966037172283563 1107 20-21 0.29135794877686294 1107 22-23 0.22123569254118536 1107 24-25 0.17058307414761487 1107 26-27 0.1698158378900061 1107 28-29 0.12556340127437693 1107 30-31 0.16724533888872628 1107 32-33 0.20654828966404182 1107 34-35 0.3122075041170689 1107 36-37 0.3288754550304773 1107 38-39 0.19692652129631227 1107 40-41 0.10598831910535012 1107 42-43 0.16323407203115892 1107 44-45 -0.030889120567074713 1107 46-47 0.11310961164822686 1107 48-49 0.11970176537922583 1107 50-51 0.14193157355527575 1107 52-53 0.07441414562398307 1107 54-55 0.10152236250294777 1107 56-57 0.025513544281395184 1107 58-59 0.14391383654174916 1107 60-61 0.1277873531571032 1107 62-63 0.13633956579562323 1107 64-65 0.17075881629891398 1107 66-67 0.15221720865158517 1107 68-69 0.25273710618662903 1107 70-71 0.25067968983396227 1107 72-73 0.32285978111375613 1107 74-75 0.35248980374444727 1107 76-77 0.30302223078684776 1107 78-79 0.124235913688171 1107 80-81 0.1937259573720702 1107 82-83 0.12600180419849494 1107 84-85 0.31692494178236785 1107 86-87 0.33883680795222304 1107 88-89 0.4251234788419396 1107 90-91 0.5704371188612356 1107 92-93 0.15738787102581853 1107 94-95 0.12172285520645687 1107 96-97 0.26054544110649047 1107 98-99 0.134911567510823 1107 100-101 0.2839058091093065 1108 1 0.12284471205260417 1108 2 -0.002142760284129963 1108 3 0.019727533366065586 1108 4 -0.06014268061869643 1108 5 -0.02139453879268416 1108 6 -0.12156819314482448 1108 7 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2316 98-99 -0.42400731834212024 2316 100-101 -0.35615220239104417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 3.0 5 19.0 6 53.0 7 115.0 8 331.0 9 724.0 10 1278.0 11 2293.0 12 3719.0 13 5469.0 14 7438.0 15 11104.0 16 16606.0 17 25826.0 18 23158.0 19 11361.0 20 7820.0 21 7482.0 22 7362.0 23 7722.0 24 8719.0 25 9828.0 26 11900.0 27 14351.0 28 17223.0 29 21536.0 30 26798.0 31 33548.0 32 42436.0 33 55943.0 34 78274.0 35 117306.0 36 173217.0 37 267702.0 38 219012.0 39 19694.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38519517790158 19.975230907754618 16.014880545206378 19.624693369137425 2 15.551421729521058 21.39064099311503 41.21413068634149 21.84380659102242 3 19.29325890325654 26.8579749748882 31.66090997369922 22.187856148156044 4 11.840919628719151 17.94338531721194 38.586358745229155 31.62933630883975 5 11.658452567055296 39.326881386810506 34.11265846450037 14.90200758163383 6 28.786036328006638 38.473345702999175 17.251786854698313 15.48883111429587 7 26.021789805285128 36.29182019394342 20.35030066559194 17.336089335179505 8 26.631392175934504 34.375211253267715 19.151058115426 19.842338455371777 9 26.908317871251025 16.61458735128764 21.20398257642696 35.27311220103437 10-11 24.429148934647284 28.233855080854823 26.195087074123354 21.141908910374536 12-13 26.45195874452016 26.15060731578512 27.841245299585793 19.556188640108928 14-15 23.049849011076233 26.250440400929868 28.777566314637536 21.92214427335636 16-17 19.53169176786278 29.898153059901684 29.323902059605032 21.2462531126305 18-19 19.302325396440366 26.495633370103 34.86861935600858 19.333421877448053 20-21 20.457547736279572 25.144129405898152 35.485140892508774 18.913181965313505 22-23 20.01961237329202 25.362035583016766 35.41389384743307 19.20445819625814 24-25 19.745199121343372 25.42338238264828 35.79909398476507 19.032324511243274 26-27 19.57869713682183 25.580034063393857 35.875442344942165 18.965826454842144 28-29 19.23649090101486 25.96074888328016 35.773528063240164 19.029232152464818 30-31 19.408538568782475 25.742199833462838 35.62567153685808 19.223590060896612 32-33 19.22895837922914 25.80359748905857 35.53182537934128 19.435618752371006 34-35 19.687213938222186 25.718539467478728 35.531586787415385 19.062659806883694 36-37 19.752071176743332 25.421055101214673 35.54240295472241 19.284470767319586 38-39 19.587402982558135 26.002861512497844 35.46454245624025 18.94519304870377 40-41 19.490666419589804 26.09481627701028 35.33975918754128 19.074758115858636 42-43 19.429182748616505 26.081055015679276 34.83237985018682 19.657382385517398 44-45 19.74451576575681 26.33708903534898 34.302321419908274 19.61607377898594 46-47 20.024384094825976 27.000613181249538 33.35880964897561 19.61619307494888 48-49 20.140300005487614 27.25344109205115 32.69918250453126 19.907076397929977 50-51 20.208577061613184 27.621707729662624 32.34216945275761 19.82754575596659 52-53 20.347087765677184 28.052059188317894 31.576023569710426 20.024829476294492 54-55 19.94996711397493 27.862018669082282 31.763366956344697 20.42464726059809 56-57 20.381277850636685 28.011328344641267 31.358812830201284 20.24858097452077 58-59 20.420764814371505 27.607034326220376 31.70520854127282 20.266992318135294 60-61 20.403665726349377 27.528776174528403 32.275085356261485 19.792472742860735 62-63 20.22937432448661 27.522612549776245 32.35445693694095 19.8935561887962 64-65 20.165797595231023 27.738151619662048 32.02163393382968 20.074416851277242 66-67 20.1014492868885 27.849841376134606 31.687870861324807 20.36083847565209 68-69 20.41472048558229 27.68334397718425 31.679878031807483 20.222057505425976 70-71 20.41591344521174 27.997490012939636 31.513619224783017 20.072977317065604 72-73 20.418259599149657 27.723745543301654 31.53501296747117 20.322981890077518 74-75 20.205356070613668 28.22971948747273 31.03929847611337 20.525625965800234 76-77 20.081924514286488 27.899150374151905 31.488646603206515 20.53027850835509 78-79 20.14717940601745 27.934183621936775 31.32354099049052 20.595095981555254 80-81 20.09250208966762 28.26419602076386 31.20734672258201 20.435955166986513 82-83 20.154600414118192 28.238693368422435 31.13656635017284 20.470139867286534 84-85 19.843189308528235 28.175541763163924 31.084258010332626 20.897010917975212 86-87 19.782849535183164 28.106606053713406 31.049637459568608 21.06090695153482 88-89 19.778475349875176 27.949811393082584 30.979531198677883 21.292182058364357 90-91 19.66518826548225 27.638214337415906 31.3256931596371 21.370904237464742 92-93 19.76464899360245 27.60821119873767 31.55812673578525 21.069013071874632 94-95 19.508071167199653 27.61061320510873 31.430390407968332 21.45092521972328 96-97 19.4677888970452 27.483085820720436 31.51668115449861 21.532444127735754 98-99 19.43131071725394 27.728821782060102 31.063136002672252 21.776731498013703 100-101 19.1990915852315 27.564939326325334 31.468776225305955 21.767192863137215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1051.0 1 772.0 2 799.5 3 1127.5 4 849.5 5 672.5 6 1628.5 7 4284.0 8 7130.5 9 7952.5 10 6343.0 11 4586.5 12 4281.5 13 4447.5 14 4699.5 15 4830.0 16 4850.0 17 5006.5 18 5215.0 19 5378.0 20 5650.0 21 6112.0 22 6868.5 23 7934.0 24 9083.5 25 10486.0 26 12250.0 27 14211.5 28 15763.5 29 17590.0 30 20276.0 31 22856.5 32 25531.0 33 27987.0 34 30960.0 35 33769.0 36 36897.0 37 39894.0 38 40652.0 39 40557.0 40 40554.0 41 40725.5 42 40702.0 43 41609.5 44 43060.0 45 43926.5 46 43910.0 47 45365.5 48 51066.5 49 48431.5 50 39705.5 51 35180.5 52 31600.5 53 29851.0 54 28265.0 55 25524.0 56 22590.5 57 20073.0 58 18009.0 59 15857.5 60 13157.0 61 10386.5 62 7944.0 63 5583.5 64 3653.5 65 2557.0 66 1874.5 67 1455.0 68 1018.0 69 646.0 70 474.0 71 359.5 72 292.0 73 284.0 74 250.0 75 219.0 76 173.0 77 104.5 78 68.5 79 50.5 80 39.5 81 25.5 82 14.0 83 9.5 84 7.5 85 7.0 86 4.0 87 1.0 88 0.5 89 0.0 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013122555923959162 2 0.0 3 0.0 4 0.0 5 0.031176011649648436 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0032607563204989434 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.964798147254165E-4 24-25 2.3859192589016659E-4 26-27 0.0012327249504325273 28-29 9.14602382578972E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.760104566888054E-4 42-43 0.01149217776370969 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.976532098169443E-5 54-55 3.1812256785355547E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.976532098169443E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.169491727620276E-4 84-85 7.953064196338887E-5 86-87 0.0 88-89 0.0 90-91 0.012963494640032385 92-93 0.0016701434812311663 94-95 0.0 96-97 0.0 98-99 9.543677035606663E-4 100-101 0.019246415355140104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1257377.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.24918911336653 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21503898002916 36.55030489783325 2 13.826364555358639 13.618744854744111 3 4.933032811241098 7.288435974697649 4 2.288372884598811 4.508020358220277 5 1.2799849345735648 3.1519110052536785 6 0.7669581990457921 2.266324163211194 7 0.4986204641285439 1.7189657473562876 8 0.35916680961373193 1.4150939303928955 9 0.2584773809614206 1.145682127484702 >10 1.4023083023490182 12.680635140848523 >50 0.10922056871203861 3.6799180736570634 >100 0.055623197003525096 4.990507181780765 >500 0.004716582360835781 1.6380298857404516 >1k 0.0016264077106330282 1.5526440736035656 >5k 0.0 0.0 >10k+ 4.8792231318990837E-4 3.7947825851755326 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19570 1.55641466322352 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16125 1.2824316016596453 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11681 0.9289974287743453 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3641 0.28957106738869887 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3172 0.2522711963078695 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2782 0.22125424594214785 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1704 0.1355202139056146 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1590 0.1264537207217883 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.12017080000668058 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1395 0.11094524553892746 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.10808214242824547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.953064196338887E-5 0.0 3 0.0 0.0 0.0 1.5906128392677773E-4 0.0 4 0.0 0.0 0.0 1.5906128392677773E-4 0.0 5 0.0 0.0 0.0 1.5906128392677773E-4 0.0 6 0.0 0.0 0.0 1.5906128392677773E-4 0.0 7 0.0 0.0 0.0 1.5906128392677773E-4 0.0 8 0.0 0.0 0.0 1.5906128392677773E-4 0.0 9 0.0 0.0 0.0 1.5906128392677773E-4 0.0 10-11 0.0 0.0 0.0 1.5906128392677773E-4 0.0 12-13 0.0 0.0 0.0 1.5906128392677773E-4 0.0 14-15 0.0 0.0 0.0 1.5906128392677773E-4 0.0 16-17 0.0 0.0 0.0 1.9882660490847215E-4 0.0 18-19 0.0 0.0 0.0 2.3859192589016659E-4 0.0 20-21 0.0 0.0 0.0 2.78357246871861E-4 0.0 22-23 0.0 0.0 0.0 3.1812256785355547E-4 0.0 24-25 0.0 0.0 0.0 3.9765320981694435E-4 0.0 26-27 0.0 0.0 0.0 5.964798147254165E-4 0.0 28-29 0.0 0.0 0.0 0.001471316876322694 0.0 30-31 0.0 0.0 0.0 0.003658409530315888 0.0 32-33 0.0 0.0 0.0 0.007316819060631776 0.0 34-35 0.0 0.0 0.0 0.010259452813277163 0.0 36-37 0.0 0.0 0.0 0.014235984911446606 0.0 38-39 0.0 0.0 0.0 0.019445241960048577 0.0 40-41 0.0 0.0 0.0 0.02493285625552241 0.0 42-43 0.0 0.0 0.0 0.037140809796902596 0.0 44-45 0.0 0.0 0.0 0.05058148828871532 0.0 46-47 0.0 0.0 0.0 0.06298826843500398 0.0 48-49 0.0 0.0 0.0 0.08155867333345529 0.0 50-51 0.0 0.0 0.0 0.10327053858946045 0.0 52-53 0.0 0.0 0.0 0.14788722873092158 0.0 54-55 0.0 0.0 0.0 0.19015776493446276 0.0 56-57 0.0 0.0 0.0 0.26372360875059747 0.0 58-59 0.0 0.0 0.0 0.34484486355325417 0.0 60-61 0.0 0.0 0.0 0.43312387613261577 0.0 62-63 0.0 0.0 0.0 0.551783593941992 0.0 64-65 0.0 0.0 0.0 0.6224465693264629 0.0 66-67 0.0 0.0 0.0 0.7664765619221602 0.0 68-69 0.0 0.0 0.0 0.9502718754995518 0.0 70-71 0.0 0.0 0.0 1.098994175971089 0.0 72-73 0.0 0.0 0.0 1.3278833635417222 0.0 74-75 0.0 0.0 0.0 1.5611865017413233 0.0 76-77 0.0 0.0 0.0 1.7387784252455707 0.0 78-79 0.0 0.0 0.0 1.933032018241148 0.0 80-81 0.0 0.0 0.0 2.2530235561808434 0.0 82-83 0.0 0.0 0.0 2.528915353151839 0.0 84-85 0.0 0.0 0.0 2.8873997218018146 0.0 86-87 0.0 0.0 0.0 3.3577041730523147 0.0 88-89 0.0 0.0 0.0 3.943924534964454 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGACG 75 6.9118105E-6 38.008797 4 GGTATCA 10540 0.0 34.985203 1 GTATCAA 15375 0.0 30.071909 1 TAACTCG 85 6.3988473E-4 27.947647 4 CGAAGTA 70 0.008390529 27.143742 7 ATGGGCG 150 1.0581061E-6 25.339197 5 GTACATG 8505 0.0 24.695126 1 TACATGG 8535 0.0 24.326077 2 TATCAAC 19155 0.0 24.10895 2 ATCAACG 19270 0.0 23.965069 3 GAGTACT 12795 0.0 23.964245 12-13 TCAACGC 19400 0.0 23.779987 4 CAACGCA 19525 0.0 23.65208 5 AACGCAG 19870 0.0 23.234943 6 ACATGGG 8560 0.0 22.97845 3 TTACGAA 105 0.002193383 22.624283 4 GTACTTT 13545 0.0 22.495255 14-15 AGTACTT 13080 0.0 22.38892 12-13 CATGGGA 6385 0.0 21.802229 4 ATGGGAT 2485 0.0 21.031027 5 >>END_MODULE