FastQCFastQC Report
Thu 26 May 2016
SRR937594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293043
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121520.9397985991185135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94080.7275860122207846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63350.4899295692409301No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30880.23881649720852283No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.1673571567225529No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17510.13541699695988454No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15850.12257906349595489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15800.12219237875306543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15240.11786150963270363No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15110.11685612930119106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147200.028.227211
GTACATG101400.025.9738371
TACATGG101100.025.6657642
GGTATCA103550.025.2968121
GAGTACT85250.024.76879312-13
ACATGGG101000.024.5129363
TATCAAC176100.023.6405412
ATCAACG176100.023.6117253
TCAACGC176000.023.5738834
CAACGCA177200.023.3883275
GTACTTT90950.023.16470514-15
AGTACTT88950.023.12434212-13
CATGGGG22100.023.0102334
AACGCAG180300.022.9781766
AGTACAT77400.022.6567762
CATGGGA73450.021.3526444
TTACGTT1353.7823906E-421.1225834
ACGCAGA196450.020.8473117
CGCAGAG196150.020.8307538
TACTTTT99850.020.45767414-15
Produced by FastQC (version 0.11.5)