##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1293043 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81706718183386 33.0 31.0 34.0 30.0 34.0 2 31.905194181477338 33.0 31.0 34.0 30.0 34.0 3 31.928312515515724 33.0 31.0 34.0 30.0 34.0 4 35.52453630699056 37.0 35.0 37.0 33.0 37.0 5 35.534929619509946 37.0 35.0 37.0 33.0 37.0 6 35.64825995732547 37.0 35.0 37.0 33.0 37.0 7 35.596083811597914 37.0 35.0 37.0 33.0 37.0 8 35.59470798728271 37.0 35.0 37.0 33.0 37.0 9 37.2065801369328 39.0 37.0 39.0 34.0 39.0 10-11 37.10933124420456 39.0 37.0 39.0 34.0 39.0 12-13 36.985495068609474 39.0 37.0 39.0 33.0 39.0 14-15 38.080211562956535 40.0 38.0 41.0 33.0 41.0 16-17 38.04960739898054 40.0 38.0 41.0 33.0 41.0 18-19 38.0976452445897 40.0 38.0 41.0 33.5 41.0 20-21 37.94727166845959 40.0 38.0 41.0 33.0 41.0 22-23 37.84235327054088 40.0 38.0 41.0 33.0 41.0 24-25 37.7034994195862 40.0 37.0 41.0 32.5 41.0 26-27 37.78769924898089 40.0 37.0 41.0 33.0 41.0 28-29 37.734712225347494 40.0 37.0 41.0 33.0 41.0 30-31 37.48590186095899 40.0 37.0 41.0 33.0 41.0 32-33 37.195124601424695 39.0 36.0 41.0 32.0 41.0 34-35 36.916143546657 39.0 35.5 41.0 31.0 41.0 36-37 36.87942241673324 39.0 36.0 41.0 31.0 41.0 38-39 36.803943488344935 39.0 35.0 41.0 31.0 41.0 40-41 36.592761416287004 39.0 35.0 41.0 30.5 41.0 42-43 36.29664326708392 39.0 35.0 41.0 30.0 41.0 44-45 35.95398258217244 39.0 35.0 41.0 29.5 41.0 46-47 35.44107775224799 39.0 35.0 41.0 26.5 41.0 48-49 34.97336051469286 39.0 35.0 40.0 24.0 41.0 50-51 34.02847159761895 38.0 34.0 39.5 22.5 40.5 52-53 34.10688778331424 38.0 34.0 39.5 23.0 40.5 54-55 34.40711716470373 38.0 34.0 40.0 22.5 41.0 56-57 34.162583920256324 38.0 34.0 40.0 20.5 41.0 58-59 33.95221736632115 38.0 34.0 40.0 19.5 41.0 60-61 33.64845793991383 38.0 34.0 40.0 18.0 41.0 62-63 33.30882925007134 37.5 33.0 40.0 15.5 41.0 64-65 32.95332019120787 37.0 33.0 40.0 12.5 41.0 66-67 32.55203075226423 36.0 32.0 39.0 8.0 41.0 68-69 32.0815510389059 36.0 32.0 39.0 7.0 40.5 70-71 31.64849506164915 35.0 31.0 39.0 2.0 40.0 72-73 31.182371351919464 35.0 31.0 38.0 2.0 40.0 74-75 30.71209619479012 35.0 30.5 37.0 2.0 39.5 76-77 30.2615984155206 35.0 30.0 37.0 2.0 39.0 78-79 29.740927409219957 34.0 29.5 36.0 2.0 39.0 80-81 29.21121571363056 34.0 29.0 36.0 2.0 37.5 82-83 28.70986695724736 34.0 29.0 35.0 2.0 37.0 84-85 28.244167440680627 34.0 27.5 35.0 2.0 36.5 86-87 27.74817078782376 34.0 26.5 35.0 2.0 36.0 88-89 27.268204924352865 33.0 26.0 35.0 2.0 36.0 90-91 26.752518284388067 33.0 25.0 35.0 2.0 35.0 92-93 26.225949562388877 33.0 24.5 35.0 2.0 35.0 94-95 25.618210685955532 32.0 21.5 35.0 2.0 35.0 96-97 24.885235835157843 32.0 18.0 34.0 2.0 35.0 98-99 24.00884464012411 31.5 2.0 34.0 2.0 35.0 100-101 21.531211645707064 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3129048863499939 1101 2 0.45638276291302304 1101 3 0.3627166987839132 1101 4 0.15774015057505864 1101 5 0.24554225801107066 1101 6 0.24168231047632815 1101 7 0.24211952574554374 1101 8 0.24643720865740448 1101 9 0.15587139809296957 1101 10-11 0.28061085211691505 1101 12-13 0.34728609916250974 1101 14-15 0.6073674685415966 1101 16-17 0.6118158825694806 1101 18-19 0.6719345877076464 1101 20-21 0.5555549081409055 1101 22-23 0.5768508137671162 1101 24-25 0.5088761694779578 1101 26-27 0.7069795474221934 1101 28-29 0.5861753568959642 1101 30-31 0.469064599923378 1101 32-33 0.523254524523324 1101 34-35 0.6399598830907678 1101 36-37 0.6657961284310048 1101 38-39 0.6459251188651365 1101 40-41 0.7455218691267333 1101 42-43 0.7553343321715076 1101 44-45 0.8048387609373862 1101 46-47 0.7837352377978135 1101 48-49 0.9906162814130539 1101 50-51 1.0828439794441849 1101 52-53 1.1295434167779916 1101 54-55 1.1063085827329218 1101 56-57 1.293903155347813 1101 58-59 1.2142418661346284 1101 60-61 1.3866301677283701 1101 62-63 1.2348704846432597 1101 64-65 1.1737766114784165 1101 66-67 1.1852719382706383 1101 68-69 1.3344284949926433 1101 70-71 1.2710220771120184 1101 72-73 1.129089969338093 1101 74-75 1.3046262612702542 1101 76-77 1.3362644466375322 1101 78-79 1.365407081742049 1101 80-81 1.2980075695413973 1101 82-83 1.3382950992289224 1101 84-85 1.688825794407684 1101 86-87 1.6898753641807005 1101 88-89 1.8495939590348591 1101 90-91 1.7590892846801864 1101 92-93 2.045466944452894 1101 94-95 1.981221705654189 1101 96-97 2.052553998842928 1101 98-99 2.472823918545231 1101 100-101 2.846534894072761 1102 1 0.2014578044034856 1102 2 0.4109626929575114 1102 3 0.4262204805011507 1102 4 0.23762976947729442 1102 5 0.23146308546504457 1102 6 0.3262141340744762 1102 7 0.25448975480460945 1102 8 0.36236960552884767 1102 9 0.3183832129514883 1102 10-11 0.32291502384479287 1102 12-13 0.48294882850552057 1102 14-15 0.8046912466782885 1102 16-17 0.656254910199408 1102 18-19 0.5092271418923602 1102 20-21 0.6561436297876497 1102 22-23 0.6105575704224222 1102 24-25 0.6430200518356557 1102 26-27 0.6828259821743359 1102 28-29 0.4908686710816923 1102 30-31 0.5376964152439072 1102 32-33 0.5038716595813568 1102 34-35 0.6059287592402498 1102 36-37 0.6730989622645325 1102 38-39 0.6701884107012859 1102 40-41 0.6918961272691249 1102 42-43 0.9164935743265445 1102 44-45 1.0223260028152197 1102 46-47 1.0458966013335527 1102 48-49 1.1643498055808195 1102 50-51 0.889169572882146 1102 52-53 1.132212949169471 1102 54-55 1.0998967961723167 1102 56-57 1.3060604281993378 1102 58-59 1.2623692471534724 1102 60-61 1.2638619969090996 1102 62-63 1.2694167940760934 1102 64-65 1.1586073919039634 1102 66-67 1.0969843853083106 1102 68-69 1.0343318104681387 1102 70-71 1.268777735250179 1102 72-73 1.2635146651355633 1102 74-75 1.261972215727944 1102 76-77 1.1497379091659035 1102 78-79 1.0658352079391769 1102 80-81 1.3554576198808732 1102 82-83 1.5475191589971864 1102 84-85 1.5565198324038825 1102 86-87 1.5415923927846897 1102 88-89 1.5397775681926014 1102 90-91 1.8025785516873256 1102 92-93 1.9574217043730435 1102 94-95 2.0022483037641976 1102 96-97 1.8205688908584925 1102 98-99 2.000358554609086 1102 100-101 2.2929608873390137 1103 1 0.21335325657918602 1103 2 0.25025259301288116 1103 3 0.21156584113840182 1103 4 0.19629551065585815 1103 5 0.1656867464373306 1103 6 0.3015179487828661 1103 7 0.18236533525539045 1103 8 0.15736994905063284 1103 9 0.086990979776985 1103 10-11 0.2754898206617611 1103 12-13 0.21413419084017704 1103 14-15 0.48981892564302854 1103 16-17 0.34718785474690605 1103 18-19 0.23126219525948244 1103 20-21 0.25165885184417647 1103 22-23 0.19798916917280707 1103 24-25 0.28431334063240143 1103 26-27 0.1665498694668628 1103 28-29 0.2320616313099464 1103 30-31 0.2487299308458546 1103 32-33 0.3370302009474315 1103 34-35 0.23798131201125727 1103 36-37 0.305544291782752 1103 38-39 0.5002638440439142 1103 40-41 0.5896246399814444 1103 42-43 0.5512922806639082 1103 44-45 0.35621604351244684 1103 46-47 0.5668539561349704 1103 48-49 0.7318321344160381 1103 50-51 0.4067599090081089 1103 52-53 0.3263355945297022 1103 54-55 0.3830825161737721 1103 56-57 0.5802716415587028 1103 58-59 0.7105176647304745 1103 60-61 0.5035159290211055 1103 62-63 0.3504194836496026 1103 64-65 0.4796039991211529 1103 66-67 0.6486652171968288 1103 68-69 0.8068044499682898 1103 70-71 0.6915320626143107 1103 72-73 0.7251922840850362 1103 74-75 0.7587721587437422 1103 76-77 0.6173958382549252 1103 78-79 0.6424986616667923 1103 80-81 0.8423752494188967 1103 82-83 0.7809578726899638 1103 84-85 0.9285837314973904 1103 86-87 1.034336505427504 1103 88-89 1.0451145327532778 1103 90-91 1.1650455634923524 1103 92-93 1.0815381461339761 1103 94-95 0.8727067436903226 1103 96-97 0.9197726780851418 1103 98-99 0.9770571688017924 1103 100-101 1.1351757743607607 1104 1 -0.03757074314343711 1104 2 0.07525490445684113 1104 3 0.16299084633915584 1104 4 0.12298286474595699 1104 5 0.06068478170996627 1104 6 0.030234172808015103 1104 7 0.07490052698966565 1104 8 0.19879333624061246 1104 9 0.1288188696597956 1104 10-11 0.25620428798950456 1104 12-13 0.10140189072229333 1104 14-15 0.24544122414290115 1104 16-17 0.15084860411703715 1104 18-19 0.33847014431525935 1104 20-21 0.32983650942686893 1104 22-23 0.20528784240397613 1104 24-25 0.21932102618356453 1104 26-27 0.2723755750351273 1104 28-29 0.2928875680226142 1104 30-31 0.2846197211978918 1104 32-33 0.2988231880264678 1104 34-35 0.3143083697742455 1104 36-37 0.2991779972585604 1104 38-39 0.3107734018646866 1104 40-41 0.3584929570191022 1104 42-43 0.5515427834029722 1104 44-45 0.4854430966525456 1104 46-47 0.588843300378322 1104 48-49 0.5606772214419067 1104 50-51 0.6730498103094575 1104 52-53 0.47043158879731806 1104 54-55 0.4178079166314319 1104 56-57 0.5409632255912413 1104 58-59 0.5898441099608078 1104 60-61 0.768260630035833 1104 62-63 0.664655955923827 1104 64-65 0.7079789962318515 1104 66-67 0.7220385385106525 1104 68-69 0.7695361722251874 1104 70-71 0.7927851541706055 1104 72-73 0.7369488659217112 1104 74-75 0.7592931004260102 1104 76-77 0.7465818632647476 1104 78-79 0.6719227654043962 1104 80-81 0.9276325709176376 1104 82-83 0.8394252697729243 1104 84-85 0.8312181997497063 1104 86-87 0.8135687593165066 1104 88-89 0.7978180553938721 1104 90-91 0.9802999379000461 1104 92-93 0.8670731298382961 1104 94-95 0.7586375621726269 1104 96-97 0.7347817505026804 1104 98-99 1.0442872499334754 1104 100-101 1.2999083402946994 1105 1 -0.007879973554970832 1105 2 0.02046365989577481 1105 3 0.11612134755149839 1105 4 -0.01832314382347988 1105 5 0.09952624714989611 1105 6 -0.03685985848039053 1105 7 0.16103996300122247 1105 8 0.19162224646082393 1105 9 0.22645729261790848 1105 10-11 0.18636504794566378 1105 12-13 0.23355222121021058 1105 14-15 0.07132723071944724 1105 16-17 0.16092931624378082 1105 18-19 0.12504137359749024 1105 20-21 0.3559932171692566 1105 22-23 0.1614707898912684 1105 24-25 0.194619129614658 1105 26-27 0.3169681946916896 1105 28-29 0.08300946658440722 1105 30-31 0.12079735430646821 1105 32-33 0.020700160159186964 1105 34-35 -0.005319955864315773 1105 36-37 -0.10968372925022862 1105 38-39 0.06767379047863642 1105 40-41 0.056387134452918986 1105 42-43 -0.14780288371763106 1105 44-45 0.011584857704789897 1105 46-47 -0.1468211630355043 1105 48-49 0.01796290251274968 1105 50-51 -0.22160757833770361 1105 52-53 -0.3244101423615433 1105 54-55 -0.2848409690097782 1105 56-57 -0.08837546640729244 1105 58-59 0.016264875895643627 1105 60-61 -0.0921472485306083 1105 62-63 0.011977176902341569 1105 64-65 0.12440042646402105 1105 66-67 0.20574664625968353 1105 68-69 0.3342769301884516 1105 70-71 0.37479598221723975 1105 72-73 0.32640978863071624 1105 74-75 0.37836341629939696 1105 76-77 0.21837745897338934 1105 78-79 0.30904370073505305 1105 80-81 0.5367367659553395 1105 82-83 0.5006087154832244 1105 84-85 0.6450820852248427 1105 86-87 0.4744134133836688 1105 88-89 0.406306114649194 1105 90-91 0.2829604487446993 1105 92-93 0.19605974424690586 1105 94-95 0.3201697639861578 1105 96-97 0.32191098955007647 1105 98-99 0.8649718083418172 1105 100-101 1.2359780250856112 1106 1 -5.97795591922079E-4 1106 2 0.006831540381302403 1106 3 0.08961069079535378 1106 4 0.027470396689835752 1106 5 0.06882714336140339 1106 6 -0.03661577134422345 1106 7 -0.019999171812806082 1106 8 -0.011414873899127542 1106 9 0.07663043274010306 1106 10-11 -0.1768171278117805 1106 12-13 -0.32073768372813305 1106 14-15 -0.22943039516555075 1106 16-17 -0.18547664771660521 1106 18-19 -0.31647402521295476 1106 20-21 -0.18334598307777128 1106 22-23 -0.1607764292063436 1106 24-25 -0.14575008893890384 1106 26-27 0.012468389520279288 1106 28-29 0.11005755616144342 1106 30-31 0.32622682268173975 1106 32-33 0.40806901846367083 1106 34-35 -0.025576614958403354 1106 36-37 0.2060657726502697 1106 38-39 0.21309825947882644 1106 40-41 0.23484497428176354 1106 42-43 0.12152815578478027 1106 44-45 0.18408131035408815 1106 46-47 0.3531446500613882 1106 48-49 0.4943165935954852 1106 50-51 0.5630660286378983 1106 52-53 0.595796155380647 1106 54-55 0.5871334900651846 1106 56-57 0.6022360012768857 1106 58-59 0.7065576334185977 1106 60-61 0.4853849717065657 1106 62-63 0.7281391272467843 1106 64-65 0.6832261614824375 1106 66-67 0.7285680582890635 1106 68-69 0.805938193118827 1106 70-71 0.9019403832847495 1106 72-73 0.8881233979589602 1106 74-75 0.9844622862935886 1106 76-77 1.3236455504619506 1106 78-79 1.3431997253496206 1106 80-81 1.168551171507584 1106 82-83 0.9846059364188413 1106 84-85 1.061480770683282 1106 86-87 1.058298148380029 1106 88-89 1.3014085650781873 1106 90-91 1.7070978727431694 1106 92-93 1.6123665294281686 1106 94-95 1.2238314386297695 1106 96-97 1.0922786641789841 1106 98-99 1.2007816703443623 1106 100-101 0.8943218350505404 1107 1 0.3334521148359393 1107 2 0.278789212338971 1107 3 0.1836905974736851 1107 4 0.21731333298946964 1107 5 0.250537762867296 1107 6 0.3445282523194848 1107 7 0.2772553380401277 1107 8 0.19532595016453058 1107 9 0.33962255872435065 1107 10-11 0.30580171149997426 1107 12-13 0.3985849009487765 1107 14-15 0.4894255811707424 1107 16-17 0.4088907230287191 1107 18-19 0.33291563441292027 1107 20-21 0.5973564291207012 1107 22-23 0.5572379856584675 1107 24-25 0.4489297427488026 1107 26-27 0.36441606272779126 1107 28-29 0.5096201108421141 1107 30-31 0.4602309055047229 1107 32-33 0.5927667645787338 1107 34-35 0.6073317714932998 1107 36-37 0.555551253631819 1107 38-39 0.4563136908832348 1107 40-41 0.5423815322120262 1107 42-43 0.7431712837271292 1107 44-45 0.5686499059463088 1107 46-47 0.6536612521527942 1107 48-49 0.7071941505675241 1107 50-51 0.5128306413946362 1107 52-53 0.36012144805239643 1107 54-55 0.4243871666889447 1107 56-57 0.38678793228551456 1107 58-59 0.4313753645355405 1107 60-61 0.35699685355466215 1107 62-63 0.2528735354457581 1107 64-65 0.33081187993429495 1107 66-67 0.46836725005675817 1107 68-69 0.638400801959385 1107 70-71 0.6483720793227512 1107 72-73 0.5971847652880449 1107 74-75 0.6284567869810012 1107 76-77 0.7562081468040773 1107 78-79 0.8312970465178076 1107 80-81 0.6072005070073132 1107 82-83 0.8915050740266395 1107 84-85 0.8634138623801526 1107 86-87 0.9198071264244412 1107 88-89 0.7754159808178862 1107 90-91 0.9303616314400003 1107 92-93 1.1387923928619053 1107 94-95 1.1670885314931603 1107 96-97 1.195082480368626 1107 98-99 1.2049811454099775 1107 100-101 1.1592428865857656 1108 1 0.01560673510308419 1108 2 0.06894083661038053 1108 3 0.03461484295328887 1108 4 0.12841388129509568 1108 5 0.06879221124878399 1108 6 0.035688144386817555 1108 7 0.21155468794746213 1108 8 0.052005964376306224 1108 9 0.08487361740554178 1108 10-11 0.14507038125865535 1108 12-13 0.06073884903738502 1108 14-15 0.12632578186404686 1108 16-17 0.17852985918328557 1108 18-19 0.1312798399459112 1108 20-21 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-0.44697755331226574 2316 70-71 -0.314815106120534 2316 72-73 -0.3989764329310823 2316 74-75 -0.5605809114316962 2316 76-77 -0.46580154799525175 2316 78-79 -0.5007873353379146 2316 80-81 -0.5922119171764528 2316 82-83 -0.6919262558223735 2316 84-85 -0.5587910188948513 2316 86-87 -0.3985658087930055 2316 88-89 -0.5402178912946454 2316 90-91 -0.6016848422985461 2316 92-93 -0.68885391633928 2316 94-95 -0.7920432919679286 2316 96-97 -0.429802988724159 2316 98-99 -0.08776953209679661 2316 100-101 0.48131710583986376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4740.0 3 796.0 4 755.0 5 854.0 6 885.0 7 1112.0 8 1418.0 9 1822.0 10 2261.0 11 3220.0 12 4709.0 13 6836.0 14 8076.0 15 10741.0 16 14839.0 17 20926.0 18 17662.0 19 10102.0 20 9140.0 21 9340.0 22 9814.0 23 10704.0 24 11809.0 25 13285.0 26 15583.0 27 17931.0 28 21483.0 29 25317.0 30 31370.0 31 38881.0 32 48925.0 33 64301.0 34 87914.0 35 125749.0 36 178454.0 37 243048.0 38 193837.0 39 24403.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.603784123437826 19.990923689171264 15.04441186281583 20.36088032457508 2 14.782044979055165 21.29060824548806 41.674640851503355 22.252705923953418 3 19.275330628752915 27.18250645129274 30.436144608208025 23.106018311746315 4 11.565393383892431 17.278648862420322 38.725786690240234 32.43017106344702 5 11.946523151846021 39.84552511380115 33.78192054469458 14.426031189658248 6 28.98827256748001 38.095012973646604 17.811561270642358 15.105153188231027 7 26.60815858402691 35.73999163063189 20.45132592489267 17.200523860448534 8 26.1884872417983 35.04468160289632 19.48051243583703 19.286318719468344 9 27.09878820381984 17.18834766057307 21.045929834242155 34.66693430136494 10-11 24.36910931666717 28.41052206538276 26.47749052724256 20.742878090707514 12-13 25.972092518609507 26.292784431949844 27.639023974394245 20.096099075046403 14-15 22.77900294077871 26.84211362511717 27.71252832180311 22.666355112301012 16-17 19.979310545229122 30.793308859852193 28.052382289981693 21.17499830493699 18-19 19.888603023798268 28.36755965065908 32.22026766271708 19.523569662825572 20-21 21.47477396014694 26.96419702291431 32.18870269512185 19.372326321816903 22-23 20.765371158905374 27.2515759578763 32.17856077366262 19.80449210955571 24-25 20.28793742357341 27.50574603976585 32.46896053636937 19.73735600029137 26-27 20.01297203805131 27.528113831571183 32.65481980180834 19.804094328569168 28-29 19.737405495931544 28.07294429554049 32.52997528973627 19.659674918791698 30-31 19.933849481997893 27.818245717091067 32.38709522427513 19.86080957663591 32-33 19.666398132895928 27.958085482087867 32.251293360163466 20.124223024852743 34-35 20.364060195708124 27.847024661517324 32.11952811202186 19.669387030752695 36-37 20.331722413481828 27.504190018805687 32.25291674124629 19.911170826466197 38-39 20.044929264880835 28.11402844435423 32.163589320641464 19.677452970123472 40-41 20.11925020811842 28.143459735816474 32.020021205029856 19.717268851035247 42-43 19.812988884713285 28.339603197049357 31.50395369469961 20.34345422353775 44-45 20.153767414000782 28.486839224948383 31.168354164075655 20.191039196975176 46-47 20.450666682164144 28.782227491365 30.53890545181931 20.228200374651546 48-49 20.49920854346811 29.030199346297593 30.086236793719497 20.384355316514803 50-51 20.450068427015573 29.435666981291448 29.91175007323163 20.202514518461346 52-53 20.548641185874146 29.807450467470815 29.447386523276652 20.196521823378387 54-55 20.170592132537262 29.594267360451916 29.56970333319127 20.66543717381955 56-57 20.51771430652167 29.849339589641836 29.19297726400261 20.43996883983388 58-59 20.68173788811791 29.4426504358821 29.418671497060544 20.45694017893944 60-61 20.52893686231629 29.49157282371702 29.856602627641326 20.122887686325367 62-63 20.473803415509202 29.437433010907675 29.98120916013739 20.107554413445737 64-65 20.279127059753176 29.63611995568674 29.83413515544503 20.250617829115054 66-67 20.17074682367524 29.709753641152776 29.461806631546327 20.65769290362566 68-69 20.25122920481401 29.72361146242931 29.492006149800876 20.5331531829558 70-71 20.371851700921127 29.914666321649356 29.268456134057818 20.4450258433717 72-73 20.378609016658565 29.707405033013046 29.2932360534639 20.62074989686449 74-75 20.094049570895926 30.305184581387206 29.07098735777889 20.529778489937982 76-77 20.265099642863923 29.91234496114271 29.136787495317407 20.68576790067596 78-79 20.338388813969445 29.875167115989637 29.01803787329673 20.768406196744188 80-81 20.189785619571097 30.244216500951598 28.915151587932158 20.65084629154514 82-83 20.302252759063418 30.241542712954768 28.84891041443563 20.607294113546185 84-85 20.194843396769368 30.067882249645717 28.741105147966415 20.996169205618497 86-87 20.223678553584083 30.07545035885399 28.793878760414128 20.906992327147798 88-89 20.28656775616951 29.88343466081632 28.62536254787491 21.204635035139255 90-91 20.089529551492273 29.72240105923159 29.107640769259973 21.08042862001616 92-93 20.283046640409292 29.845283433993487 28.972383275023784 20.899286650573437 94-95 20.003602319428897 29.733312158409092 28.935146629894604 21.327938892267408 96-97 19.89658837711619 29.67436340028076 29.026452550582444 21.402595672020603 98-99 20.031495391910372 29.937551572251515 28.584915528041744 21.44603750779637 100-101 20.151756941001487 29.545348909271198 28.98042420674269 21.322469942984632 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2479.0 1 1498.0 2 627.5 3 758.0 4 733.5 5 839.5 6 1376.0 7 2646.5 8 4192.5 9 4898.0 10 4412.5 11 3880.5 12 3991.5 13 4153.0 14 4257.0 15 4324.5 16 4318.0 17 4337.0 18 4430.0 19 4709.5 20 5085.5 21 5651.5 22 6539.5 23 7658.5 24 8867.5 25 10351.5 26 12359.0 27 14706.5 28 16454.5 29 18138.0 30 21008.5 31 24043.5 32 27293.5 33 30526.0 34 33583.5 35 35677.0 36 38620.5 37 41784.5 38 42147.5 39 42018.0 40 41798.0 41 42254.0 42 42873.5 43 43848.5 44 45112.0 45 45383.5 46 44958.5 47 46123.5 48 51834.0 49 50267.0 50 41927.0 51 36614.5 52 32571.5 53 30783.5 54 29399.5 55 26843.5 56 24099.5 57 21432.5 58 18915.0 59 16632.5 60 13987.5 61 11201.0 62 8750.5 63 6144.5 64 4072.5 65 2913.5 66 2096.0 67 1542.5 68 1133.0 69 816.5 70 572.0 71 441.0 72 358.0 73 303.0 74 244.0 75 174.0 76 135.5 77 103.5 78 81.5 79 51.0 80 28.5 81 24.0 82 18.5 83 11.5 84 7.0 85 5.5 86 5.5 87 2.5 88 1.5 89 1.5 90 1.5 91 1.0 92 1.0 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30725969669995506 2 0.2679725268223872 3 0.2613215492446887 4 0.2678178529252314 5 0.2716847003541259 6 0.21151655436052785 7 0.20285481611980422 8 0.19945199038237707 9 0.2108978587719047 10-11 0.21375932586928664 12-13 0.20942845674892485 14-15 0.21178723368055047 16-17 0.19570114837634944 18-19 0.2020814466340253 20-21 0.1896301979129851 22-23 0.19782791446224138 24-25 0.20022535986815598 26-27 0.19914264258806552 28-29 0.207920386251656 30-31 0.20513625610285194 32-33 0.20900310353174642 34-35 0.205097587628563 36-37 0.19148628467885445 38-39 0.18154848678659563 40-41 0.17876435663779164 42-43 0.19164095857601024 44-45 0.19508245278772632 46-47 0.19415440940479164 48-49 0.20884842963459063 50-51 0.20239079442833685 52-53 0.22083565666416355 54-55 0.19628117549068358 56-57 0.1772949546148117 58-59 0.17996307934074893 60-61 0.16932924891128912 62-63 0.17435615056885193 64-65 0.1726934061744273 66-67 0.17346677566020618 68-69 0.17551620479752025 70-71 0.1768309329233444 72-73 0.1752068570032087 74-75 0.18924351317009566 76-77 0.18239919322095244 78-79 0.18541533421549014 80-81 0.1796924000207263 82-83 0.16012615203052025 84-85 0.1591207716990077 86-87 0.1645730265737489 88-89 0.16851721095122127 90-91 0.17756563393483435 92-93 0.1725387322772715 94-95 0.17075998245998006 96-97 0.1762509058090102 98-99 0.18390726371822128 100-101 0.16727981977397505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1293043.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.23798314470458 #Duplication Level Percentage of deduplicated Percentage of total 1 75.72879568749624 40.31648348379702 2 13.393687789603037 14.26105849576644 3 4.643037973416122 7.415579321068523 4 2.154736632059457 4.588553299954356 5 1.0878471579276978 2.8957394328884782 6 0.6471694340310163 2.067239725242675 7 0.4122783754741475 1.5364208443093197 8 0.32199117563430385 1.3713728624930148 9 0.21765841694631372 1.0428925619421838 >10 1.2465388079410151 12.163811609072804 >50 0.09303004095462966 3.3897744402670917 >100 0.048999406379911105 4.611135393978168 >500 0.002333297730295831 0.8688417187925099 >1k 0.0014583110814348945 1.305397610560027 >5k 2.916622162869789E-4 1.2222478043919653 >10k+ 1.4583110814348944E-4 0.9434513954755231 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12152 0.9397985991185135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9408 0.7275860122207846 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6335 0.4899295692409301 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3088 0.23881649720852283 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2164 0.1673571567225529 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1751 0.13541699695988454 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1585 0.12257906349595489 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1580 0.12219237875306543 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1524 0.11786150963270363 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1511 0.11685612930119106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.733694857788952E-5 0.0 5 0.0 0.0 0.0 7.733694857788952E-5 0.0 6 0.0 0.0 0.0 7.733694857788952E-5 0.0 7 0.0 0.0 0.0 7.733694857788952E-5 0.0 8 0.0 0.0 0.0 7.733694857788952E-5 0.0 9 0.0 0.0 0.0 7.733694857788952E-5 0.0 10-11 0.0 0.0 0.0 7.733694857788952E-5 0.0 12-13 0.0 0.0 0.0 7.733694857788952E-5 0.0 14-15 0.0 0.0 0.0 7.733694857788952E-5 0.0 16-17 0.0 0.0 0.0 7.733694857788952E-5 0.0 18-19 0.0 0.0 0.0 7.733694857788952E-5 0.0 20-21 0.0 0.0 0.0 1.1600542286683427E-4 0.0 22-23 0.0 0.0 0.0 5.026901657562818E-4 0.0 24-25 0.0 0.0 0.0 7.347010114899504E-4 0.0 26-27 0.0 0.0 0.0 0.0010827172800904534 0.0 28-29 0.0 0.0 0.0 0.0018560867658693485 0.0 30-31 0.0 0.0 0.0 0.004176195223206034 0.0 32-33 0.0 0.0 0.0 0.0071923362177437255 0.0 34-35 0.0 0.0 0.0 0.010324482635148251 0.0 36-37 0.0 0.0 0.0 0.014036656166886947 0.0 38-39 0.0 0.0 0.0 0.01972092188736183 0.0 40-41 0.0 0.0 0.0 0.025289182184969873 0.0 42-43 0.0 0.0 0.0 0.03580700719156285 0.0 44-45 0.0 0.0 0.0 0.04825825591260306 0.0 46-47 0.0 0.0 0.0 0.06291360766811313 0.0 48-49 0.0 0.0 0.0 0.07985039940667094 0.0 50-51 0.0 0.0 0.0 0.10278080466001518 0.0 52-53 0.0 0.0 0.0 0.15401653309286698 0.0 54-55 0.0 0.0 0.0 0.19720921887361828 0.0 56-57 0.0 0.0 0.0 0.27369546101715103 0.0 58-59 0.0 0.0 0.0 0.35687134921267116 0.0 60-61 0.0 0.0 0.0 0.44975302445471654 0.0 62-63 0.0 0.0 0.0 0.5751935550480534 0.0 64-65 0.0 0.0 0.0 0.6459568629968222 0.0 66-67 0.0 0.0 0.0 0.7974599452609078 0.0 68-69 0.0 0.0 0.0 0.9942824793916367 0.0 70-71 0.0 0.0 0.0 1.1399466220380916 0.0 72-73 0.0 0.0 0.0 1.3781057551837024 0.0 74-75 0.0 0.0 0.0 1.6068684490770995 0.0 76-77 0.0 0.0 0.0 1.7748829698625646 0.0 78-79 0.0 0.0 0.0 1.9534539841289114 0.0 80-81 0.0 0.0 0.0 2.266707294343653 0.0 82-83 0.0 0.0 0.0 2.5274874849482964 0.0 84-85 0.0 0.0 0.0 2.8958433710247844 0.0 86-87 0.0 0.0 0.0 3.3624558502694804 0.0 88-89 0.0 0.0 0.0 3.9430629917179862 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14720 0.0 28.22721 1 GTACATG 10140 0.0 25.973837 1 TACATGG 10110 0.0 25.665764 2 GGTATCA 10355 0.0 25.296812 1 GAGTACT 8525 0.0 24.768793 12-13 ACATGGG 10100 0.0 24.512936 3 TATCAAC 17610 0.0 23.640541 2 ATCAACG 17610 0.0 23.611725 3 TCAACGC 17600 0.0 23.573883 4 CAACGCA 17720 0.0 23.388327 5 GTACTTT 9095 0.0 23.164705 14-15 AGTACTT 8895 0.0 23.124342 12-13 CATGGGG 2210 0.0 23.010233 4 AACGCAG 18030 0.0 22.978176 6 AGTACAT 7740 0.0 22.656776 2 CATGGGA 7345 0.0 21.352644 4 TTACGTT 135 3.7823906E-4 21.122583 4 ACGCAGA 19645 0.0 20.847311 7 CGCAGAG 19615 0.0 20.830753 8 TACTTTT 9985 0.0 20.457674 14-15 >>END_MODULE