FastQCFastQC Report
Thu 26 May 2016
SRR937594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293043
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194601.5049770193257301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163071.2611336204596444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116280.8992740380636993No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36360.2811971450292063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.24995301780373894No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31050.24013122533434694No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17680.13673172508570866No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16280.12590455228480416No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.11322129271803026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.10533292396308552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115450.033.7182851
GTATCAA166250.029.0188271
TAGACCG950.001224764525.0065545
ATAACGC1351.34017155E-524.6360873
GTACATG87700.024.2260951
GAGTACT132600.023.99962212-13
TACATGG87350.023.8786142
CATATAG5200.023.7562273
TAACGCA1401.7760967E-523.7562274
ATCAACG205450.023.5423133
TATCAAC205450.023.4729352
TCAACGC206800.023.3886264
CAACGCA208450.023.2262845
ACATGGG86600.023.2075863
AACGCAG211500.022.9058346
GGACCGC1252.257427E-422.7980426
GGACCGA2303.6307028E-922.715446
GTACTTT139700.022.5920114-15
AGGACCG2955.456968E-1222.5482835
AGTACTT134750.022.36536412-13
Produced by FastQC (version 0.11.5)