FastQCFastQC Report
Thu 26 May 2016
SRR937593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220373
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119650.9804379480699753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.7398557654094281No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61680.5054192447718853No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27590.22607842028625674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.1670800648654141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.13200882025413543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16040.13143522513198833No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14920.12225770317763503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14560.1193077854065929No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13660.11193299097898757No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12370.10136245229941993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG600.003945810331.675244
GTATCAA137350.029.1066841
GGTATCA94250.027.689581
GTACATG95950.025.7622471
TACATGG96550.025.0000272
GAGTACT80850.024.6759212-13
ATCAACG163600.024.3332733
TATCAAC164050.024.3004762
TCAACGC164500.024.1752384
CAACGCA165900.023.9712285
TCGCGAT500.001651570123.75643250-51
CGCGTCA601.7108247E-423.7515526-27
AACGCAG168500.023.5974666
AGTACTT84350.023.37045512-13
ACATGGG98300.023.0034643
GTACTTT86850.022.88915614-15
GTACGTG1050.002186565222.6363351
ATGGGAG23450.022.2874855
CATTCCG2154.417052E-822.0953739
GTACCGT1100.002875686221.5932066
Produced by FastQC (version 0.11.5)