FastQCFastQC Report
Thu 26 May 2016
SRR937593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220373
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187351.5351863733465096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152721.251420672204318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109970.9011179368930646No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.2848309492261792No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29820.24435152203465663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29610.24263073666821539No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17370.14233353245278288No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15790.12938667112432017No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.12061885997150053No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.10922889968886561No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.10185410526126028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.033.911
GTATCAA153400.030.9218751
GTACATG80750.025.4862521
TATCAAC191750.024.6737612
ATCAACG191700.024.6554163
TCAACGC192850.024.5330244
TACATGG83000.024.4949822
GAGTACT126450.024.47712512-13
CAACGCA194000.024.436575
AACGCAG197650.023.9583196
TAGGACG1000.001653309423.7509734
ACATGGG83200.023.2942243
GTACTTT134200.022.89864514-15
AGTACTT130950.022.72900212-13
ACACCGT1703.15149E-622.3511096
ACGCAGA217950.021.6885747
CATGGGG19950.021.6675534
CGCAGAG218100.021.6518758
CGGCTCG550.002877939721.59135290-91
AGAGTAC182400.021.21441510-11
Produced by FastQC (version 0.11.5)