##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937593_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1220373 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.275349421857086 33.0 31.0 34.0 31.0 34.0 2 32.46033139048471 34.0 31.0 34.0 31.0 34.0 3 32.49296649467007 34.0 31.0 34.0 31.0 34.0 4 36.11119059500661 37.0 35.0 37.0 35.0 37.0 5 36.055836207454604 37.0 35.0 37.0 35.0 37.0 6 36.126167983067475 37.0 35.0 37.0 35.0 37.0 7 36.086392439032984 37.0 35.0 37.0 35.0 37.0 8 36.06444259255162 37.0 35.0 37.0 35.0 37.0 9 37.73824314369459 39.0 37.0 39.0 35.0 39.0 10-11 37.67759938969479 39.0 37.0 39.0 35.0 39.0 12-13 37.564104171429555 39.0 37.0 39.0 35.0 39.0 14-15 38.70520365494812 40.0 38.0 41.0 34.5 41.0 16-17 38.693746092383236 40.0 38.0 41.0 34.5 41.0 18-19 38.788404856547956 40.0 38.0 41.0 35.0 41.0 20-21 38.749618354388375 40.0 38.0 41.0 35.0 41.0 22-23 38.697973488433455 40.0 38.0 41.0 34.5 41.0 24-25 38.69193639977286 40.0 38.0 41.0 34.5 41.0 26-27 38.4917725973944 40.0 38.0 41.0 34.0 41.0 28-29 38.42293053025591 40.0 38.0 41.0 34.0 41.0 30-31 38.293041143978115 40.0 37.5 41.0 34.0 41.0 32-33 38.02634850164662 40.0 37.0 41.0 34.0 41.0 34-35 37.82281851532278 40.0 36.0 41.0 33.0 41.0 36-37 37.60643713028722 40.0 36.0 41.0 33.0 41.0 38-39 37.42141050318222 40.0 36.0 41.0 33.0 41.0 40-41 37.191636081755334 40.0 36.0 41.0 33.0 41.0 42-43 36.86133829575056 40.0 35.0 41.0 31.5 41.0 44-45 36.35263644803679 39.5 35.0 41.0 30.5 41.0 46-47 35.715117427212824 39.0 35.0 41.0 28.5 41.0 48-49 35.11521354536687 39.0 35.0 41.0 24.5 41.0 50-51 34.77086104002629 39.0 35.0 41.0 23.0 41.0 52-53 34.48940815635875 39.0 35.0 40.0 21.5 41.0 54-55 34.26554012584677 38.5 34.5 40.0 19.5 41.0 56-57 33.99798954909687 38.0 34.0 40.0 18.0 41.0 58-59 33.751019565329614 38.0 34.0 40.0 15.5 41.0 60-61 33.41334165865682 38.0 33.5 40.0 11.5 41.0 62-63 33.02773865039623 37.0 33.0 40.0 8.0 41.0 64-65 32.64641097434965 37.0 33.0 39.5 6.0 41.0 66-67 32.24051253182429 36.0 32.0 39.0 2.0 40.5 68-69 31.788690015265825 36.0 31.5 39.0 2.0 40.0 70-71 31.36388915520091 35.0 31.0 38.5 2.0 40.0 72-73 30.900155935931064 35.0 31.0 37.5 2.0 40.0 74-75 30.423360726597522 35.0 30.0 37.0 2.0 39.0 76-77 29.16744757545439 33.5 28.5 35.5 2.0 38.5 78-79 29.750640173127398 34.0 30.0 36.0 2.0 38.5 80-81 29.563246237011143 34.0 30.0 36.0 2.0 37.5 82-83 29.180071994382047 34.0 29.5 35.0 2.0 37.0 84-85 28.773115678567127 34.0 29.0 35.0 2.0 37.0 86-87 28.466446324197605 34.0 29.0 35.0 2.0 36.0 88-89 28.068109504225347 34.0 29.0 35.0 2.0 36.0 90-91 27.57345582047456 34.0 27.0 35.0 2.0 35.5 92-93 27.14117896741406 33.0 26.0 35.0 2.0 35.0 94-95 26.684617735725062 33.0 25.0 35.0 2.0 35.0 96-97 26.198219724625176 33.0 25.0 35.0 2.0 35.0 98-99 25.455120278800006 32.5 22.0 34.5 2.0 35.0 100-101 23.147656495186308 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30960115573064684 1101 2 0.3060222695438597 1101 3 0.28350047999062156 1101 4 0.24858097421796543 1101 5 0.19823296877803642 1101 6 0.12617165608099867 1101 7 0.21710862631632466 1101 8 0.23197304580153144 1101 9 0.32091647735884976 1101 10-11 0.3109729407928725 1101 12-13 0.3418021075058917 1101 14-15 0.4627158134305276 1101 16-17 0.4847632255560441 1101 18-19 0.48948549857519197 1101 20-21 0.4461382147427102 1101 22-23 0.38479672741112125 1101 24-25 0.3662966841959232 1101 26-27 0.29558009415124786 1101 28-29 0.22038239470046506 1101 30-31 0.4600665783329134 1101 32-33 0.5059583565426351 1101 34-35 0.38716816489830563 1101 36-37 0.35421177431274486 1101 38-39 0.4314296419955781 1101 40-41 0.3081378067517875 1101 42-43 0.4464979349936584 1101 44-45 0.5780810324236896 1101 46-47 0.4615859542055105 1101 48-49 0.6351710464435456 1101 50-51 0.6297479356946241 1101 52-53 0.6736598080209788 1101 54-55 0.5386181715489684 1101 56-57 0.6519400009269489 1101 58-59 0.6486703588621126 1101 60-61 0.7300688595974805 1101 62-63 0.7391178233605373 1101 64-65 0.7481740475513803 1101 66-67 0.816502543287605 1101 68-69 0.6762205089772095 1101 70-71 0.6025528722295057 1101 72-73 0.7768913748281072 1101 74-75 0.7834520393636524 1101 76-77 0.7919369566152277 1101 78-79 0.8313084372002706 1101 80-81 1.0515746171745626 1101 82-83 0.9194172439897237 1101 84-85 0.8443970862443066 1101 86-87 0.957817628790373 1101 88-89 1.1616304767776242 1101 90-91 1.1066309581087062 1101 92-93 1.2995450228113086 1101 94-95 1.423689360160548 1101 96-97 1.5089791523810376 1101 98-99 1.56934811192302 1101 100-101 2.006234954274813 1102 1 0.21334439812110162 1102 2 0.1830390347261357 1102 3 0.22341402465921334 1102 4 0.15828143068528533 1102 5 0.13935078783186583 1102 6 0.09437250170248745 1102 7 0.13187229404177003 1102 8 0.18540391308944493 1102 9 0.10398511573996672 1102 10-11 0.2745007810924136 1102 12-13 0.2378322091066778 1102 14-15 0.10753270061788811 1102 16-17 0.21004720292200574 1102 18-19 0.34040474382646835 1102 20-21 0.2850862363670643 1102 22-23 0.208873800971773 1102 24-25 0.24905715454658406 1102 26-27 0.28553623542472906 1102 28-29 0.3535257086111514 1102 30-31 0.24462504611725677 1102 32-33 0.20745134124992148 1102 34-35 0.2669163716338332 1102 36-37 0.3108631552841601 1102 38-39 0.34068520058957574 1102 40-41 0.3762965816523973 1102 42-43 0.4044124893595509 1102 44-45 0.4238408296428062 1102 46-47 0.40116556252709756 1102 48-49 0.44318709642356424 1102 50-51 0.2997525634739233 1102 52-53 0.5994801870781714 1102 54-55 0.365824254975216 1102 56-57 0.3126022992504289 1102 58-59 0.39074318327995883 1102 60-61 0.5995707422622445 1102 62-63 0.6606317382514675 1102 64-65 0.6018683827288029 1102 66-67 0.6393001410494392 1102 68-69 0.45393985519821456 1102 70-71 0.39543029918421624 1102 72-73 0.3517583261733144 1102 74-75 0.45219927847259456 1102 76-77 0.3407868482831198 1102 78-79 0.314087839375329 1102 80-81 0.43557617379123315 1102 82-83 0.5570495038184973 1102 84-85 0.5932007001192332 1102 86-87 0.5968845002012131 1102 88-89 0.6308936081013812 1102 90-91 0.5262837694846247 1102 92-93 0.7523569246182475 1102 94-95 0.7909068026814268 1102 96-97 1.091987839984899 1102 98-99 1.1941172488421827 1102 100-101 1.7657907926391054 1103 1 0.19588496985057446 1103 2 0.11247755672197712 1103 3 0.11819529864232692 1103 4 0.09792940756066315 1103 5 0.1103234449790449 1103 6 0.05859207360561669 1103 7 0.07181268987172018 1103 8 0.09272278979732107 1103 9 0.13273292399828307 1103 10-11 0.19876679271074948 1103 12-13 0.21198226148440114 1103 14-15 0.3133138569611944 1103 16-17 0.18158761219650188 1103 18-19 0.28762424290495403 1103 20-21 0.194304715900536 1103 22-23 0.18192196046895504 1103 24-25 0.09013725605294098 1103 26-27 0.16617328300163337 1103 28-29 0.1770225799524141 1103 30-31 0.2740784131366567 1103 32-33 0.1642195516123124 1103 34-35 0.24432168559207668 1103 36-37 0.28241392806566523 1103 38-39 0.42438648510425736 1103 40-41 0.3626738917341257 1103 42-43 0.518048623827454 1103 44-45 0.48480177882801456 1103 46-47 0.5878350114686199 1103 48-49 0.6025100513114978 1103 50-51 0.7009314824200956 1103 52-53 0.6086936249055341 1103 54-55 0.5969727556278386 1103 56-57 0.6987177207090483 1103 58-59 0.4843222904498248 1103 60-61 0.575787894146444 1103 62-63 0.5465833042614037 1103 64-65 0.6229589597800853 1103 66-67 0.7159346341619042 1103 68-69 0.5385858994195729 1103 70-71 0.5546325332202144 1103 72-73 0.5632500549743433 1103 74-75 0.6299863616215333 1103 76-77 0.5233716749884749 1103 78-79 0.6849436969975464 1103 80-81 0.856234004401113 1103 82-83 1.0351875457318798 1103 84-85 0.8058612644542009 1103 86-87 0.9708788198789939 1103 88-89 0.881409262598865 1103 90-91 0.8386412712632314 1103 92-93 0.985331923357819 1103 94-95 0.8418470808715668 1103 96-97 1.0059673706580803 1103 98-99 1.2845508915868855 1103 100-101 1.8161512746262076 1104 1 0.19704269495808546 1104 2 0.1246448741421915 1104 3 0.16250567105012692 1104 4 0.09948175509728685 1104 5 0.08006449423900364 1104 6 0.11983884395714739 1104 7 0.11897691691281409 1104 8 0.22368452947225137 1104 9 0.22109784891109285 1104 10-11 0.2533734493811366 1104 12-13 0.1984972537133345 1104 14-15 0.1980436495229938 1104 16-17 0.172699271048522 1104 18-19 0.2563009319854217 1104 20-21 0.21614868567768752 1104 22-23 0.05672457145028176 1104 24-25 -0.02697690871375613 1104 26-27 0.09956705456503556 1104 28-29 0.1826011344019065 1104 30-31 0.1608861977798668 1104 32-33 0.19686739964409128 1104 34-35 0.16050923456975852 1104 36-37 0.17055496388107372 1104 38-39 0.23225048850522967 1104 40-41 0.3423144243125762 1104 42-43 0.4254181665696066 1104 44-45 0.3107832465122371 1104 46-47 0.022536203240797192 1104 48-49 -0.018532287193629315 1104 50-51 -0.031107128334674883 1104 52-53 0.053738094620747745 1104 54-55 0.018014035997111932 1104 56-57 0.012561747750019947 1104 58-59 -0.2072292416997641 1104 60-61 0.05496409181617423 1104 62-63 0.1065360367105086 1104 64-65 0.1981376446914993 1104 66-67 0.17644531542089226 1104 68-69 0.025107738409886338 1104 70-71 0.06578715238778798 1104 72-73 -0.006068113404950282 1104 74-75 0.07575970020820222 1104 76-77 0.018564626060896927 1104 78-79 0.07000835843453501 1104 80-81 0.3197205371326781 1104 82-83 0.028825659536256154 1104 84-85 0.10678545275508 1104 86-87 0.0845568440779374 1104 88-89 0.2131320509237682 1104 90-91 0.08771929259897959 1104 92-93 0.2757145888518231 1104 94-95 0.10412659181362471 1104 96-97 0.26692701273429265 1104 98-99 0.4489428773904365 1104 100-101 0.8052879540340747 1105 1 0.07720466351268129 1105 2 -0.013774129337150498 1105 3 0.03759517744969543 1105 4 0.04462852333192302 1105 5 0.10672979558525242 1105 6 0.10941714545403158 1105 7 0.10059629080951993 1105 8 0.14617885114915907 1105 9 0.1545644493894045 1105 10-11 0.14203305348274142 1105 12-13 0.11053574387671716 1105 14-15 0.06593554285882419 1105 16-17 0.1830716250909461 1105 18-19 0.20723547154966582 1105 20-21 0.28149670510975255 1105 22-23 0.22482667960888847 1105 24-25 0.16222756211698908 1105 26-27 0.1342643726750481 1105 28-29 0.17302905104909172 1105 30-31 0.14214977214419378 1105 32-33 0.07662978937034381 1105 34-35 0.1793424940719106 1105 36-37 0.039260855312875265 1105 38-39 0.07881370246948904 1105 40-41 0.14647108295798716 1105 42-43 0.17162587781027838 1105 44-45 0.22387355281148302 1105 46-47 0.01872221033898569 1105 48-49 -0.16577504487374028 1105 50-51 -0.14656240704183432 1105 52-53 -0.3979674057654705 1105 54-55 -0.3294392333145666 1105 56-57 -0.4386024476124888 1105 58-59 -0.3615597931927468 1105 60-61 -0.08892138748178269 1105 62-63 -0.1347843468554899 1105 64-65 -0.07549853748676583 1105 66-67 -0.23381009595979663 1105 68-69 -0.2686928906763342 1105 70-71 -0.35500979404342203 1105 72-73 -0.15487245631110724 1105 74-75 0.01325770602417009 1105 76-77 -0.07661119233367941 1105 78-79 0.1921295914672747 1105 80-81 0.25324835442312477 1105 82-83 0.1902354715895136 1105 84-85 0.37138603346146937 1105 86-87 0.47682119088713293 1105 88-89 0.48712069353556586 1105 90-91 0.4766463956190883 1105 92-93 0.526617815089125 1105 94-95 0.4563684998785895 1105 96-97 0.604128135561929 1105 98-99 0.8688015370464797 1105 100-101 1.2638334128967479 1106 1 -0.5131172004609148 1106 2 -0.29938270397953914 1106 3 -0.3914999332757816 1106 4 -0.38573749438205596 1106 5 -0.31104348440438656 1106 6 -0.29231016846262037 1106 7 -0.48815115294431877 1106 8 -0.34278271375456626 1106 9 -0.6157215178031805 1106 10-11 -0.600948968387705 1106 12-13 -0.6781180218740488 1106 14-15 -0.6777396612472657 1106 16-17 -0.4744701101650506 1106 18-19 -0.41593710764804825 1106 20-21 -0.39438090296106765 1106 22-23 -0.5461533444972275 1106 24-25 -0.5388132171160578 1106 26-27 -0.4854348605823091 1106 28-29 -0.6097127667764894 1106 30-31 -0.8070874212593395 1106 32-33 -0.7570795096802811 1106 34-35 -0.8519849695896013 1106 36-37 -0.6743394238843052 1106 38-39 -0.7440749562819207 1106 40-41 -0.7657886648898753 1106 42-43 -0.6849400667806265 1106 44-45 -0.6665389277925371 1106 46-47 -0.7585314410039103 1106 48-49 -0.9703186466923697 1106 50-51 -1.260576671289023 1106 52-53 -1.201479325001209 1106 54-55 -1.296301186400342 1106 56-57 -1.398213320908397 1106 58-59 -1.3277583030914855 1106 60-61 -1.3873938499974585 1106 62-63 -1.4864967037800483 1106 64-65 -1.7373292558247364 1106 66-67 -1.724203508585596 1106 68-69 -1.5914328541847738 1106 70-71 -1.3127440459051734 1106 72-73 -1.4212092527858076 1106 74-75 -1.4799412359654447 1106 76-77 -1.605230653217209 1106 78-79 -1.6502901874491407 1106 80-81 -1.8178703935979144 1106 82-83 -1.850594492776107 1106 84-85 -1.650480476696167 1106 86-87 -1.8013499975727179 1106 88-89 -2.143417985595846 1106 90-91 -2.249427665108051 1106 92-93 -2.03870181845582 1106 94-95 -1.8046375721353947 1106 96-97 -2.050565648452107 1106 98-99 -2.0532707848209917 1106 100-101 -1.6340724332753993 1107 1 0.07017245822624574 1107 2 0.06853432262645498 1107 3 -0.0191190566262307 1107 4 -0.031844547265123424 1107 5 -0.121796122051812 1107 6 -0.08147367549453577 1107 7 -0.0392107299809723 1107 8 -0.04653487790858435 1107 9 0.0635824830353755 1107 10-11 -0.028402369046254705 1107 12-13 0.023707638576958345 1107 14-15 -0.101571379522305 1107 16-17 -0.021491439055004946 1107 18-19 0.1504070895276186 1107 20-21 0.136192855383797 1107 22-23 0.1447864856957466 1107 24-25 0.07108604806555263 1107 26-27 0.019491639506249214 1107 28-29 0.047839453040111835 1107 30-31 0.006374249268773724 1107 32-33 -0.04618518545657224 1107 34-35 -0.016729951824565603 1107 36-37 -0.03798055202753403 1107 38-39 0.13760681277618403 1107 40-41 0.19810746294611903 1107 42-43 0.5140346934907427 1107 44-45 0.2731033136023555 1107 46-47 0.23169417318209895 1107 48-49 -0.0039249315040805755 1107 50-51 0.05928438911933398 1107 52-53 0.08467605312483073 1107 54-55 0.17329945263502822 1107 56-57 0.3770200824248562 1107 58-59 0.3511553367716118 1107 60-61 0.2973236985356138 1107 62-63 0.2060596907303207 1107 64-65 0.33461644062623463 1107 66-67 0.5130365670287702 1107 68-69 0.49920498842023164 1107 70-71 0.5273525322917187 1107 72-73 0.38943856571080104 1107 74-75 0.640132715752685 1107 76-77 0.4012121101047619 1107 78-79 0.3602712456093755 1107 80-81 0.5766273238747175 1107 82-83 0.6633021772279015 1107 84-85 0.6942245296833462 1107 86-87 0.7393830858813963 1107 88-89 0.7810309006103182 1107 90-91 0.688968493450794 1107 92-93 0.5306026161171502 1107 94-95 0.4300958799929795 1107 96-97 0.5574053057997581 1107 98-99 0.6535807731891801 1107 100-101 1.295470121492606 1108 1 0.033720660589821705 1108 2 0.04343096324458173 1108 3 0.027687528932716532 1108 4 0.0691478464178843 1108 5 -0.023407684658963035 1108 6 -0.025259276810068343 1108 7 0.02457856947310688 1108 8 4.820364614488426E-5 1108 9 0.061623008171046934 1108 10-11 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2316 100-101 0.182688877266731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 9.0 5 17.0 6 63.0 7 162.0 8 404.0 9 733.0 10 1283.0 11 2341.0 12 3672.0 13 5829.0 14 7722.0 15 11112.0 16 16698.0 17 25324.0 18 23145.0 19 11663.0 20 8206.0 21 7630.0 22 7600.0 23 7727.0 24 8595.0 25 9582.0 26 11397.0 27 13827.0 28 16707.0 29 20405.0 30 25180.0 31 31579.0 32 40192.0 33 52690.0 34 73803.0 35 109679.0 36 163874.0 37 260357.0 38 220165.0 39 20996.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39640014787625 19.919684508815607 15.980799039951998 19.703116303356154 2 15.552048431094429 21.423286159231644 41.121034306724255 21.90363110294967 3 19.288447056760518 26.843514237040644 31.668842231022808 22.199196475176034 4 11.882596550398935 17.9018218200501 38.62188035952942 31.59370127002154 5 11.749005557731737 39.283214863449594 34.094414241953906 14.873365336864758 6 28.711221896911848 38.55304894487177 17.20596899472538 15.529760163491 7 25.988038083438425 36.29849234619252 20.331161046663603 17.382308523705458 8 26.547866922653974 34.43053885984039 19.16414079957521 19.857453417930422 9 26.938731027317058 16.669657555517862 21.135177523593196 35.25643389357188 10-11 24.439454166881767 28.204286722174288 26.20194809291913 21.154311018024817 12-13 26.540938381312518 26.096663657852332 27.823406226401875 19.538991734433278 14-15 23.036440498110004 26.286430460195366 28.71540094708749 21.961728094607142 16-17 19.520507254749162 29.921999257604025 29.271009765047246 21.286483722599566 18-19 19.330032703116178 26.59690930559755 34.79145310491137 19.281604886374904 20-21 20.526069962388455 25.212353630456334 35.30040069814892 18.961175709006284 22-23 20.053631934969744 25.45292148596105 35.28125012803651 19.212196451032693 24-25 19.81100032981733 25.475541279404446 35.659521990212006 19.053936400566222 26-27 19.63079762086695 25.64676585320485 35.72071531322097 19.001721212707228 28-29 19.260681736610852 26.082923479580515 35.64605024443727 19.010344539371363 30-31 19.533339205857228 25.82211578841706 35.45802613546274 19.186518870262972 32-33 19.308072204153977 25.820384423450864 35.391392631597064 19.4801507407981 34-35 19.76354770221891 25.678378659639307 35.47722704451836 19.080846593623424 36-37 19.777124878981326 25.4060348115878 35.486260513721454 19.330579795709415 38-39 19.632776058129792 26.012139736023222 35.38419621877746 18.97088798706952 40-41 19.521087866794495 26.14473479354622 35.22583314896302 19.108344190696265 42-43 19.492900350833192 26.18840082600092 34.6146264780093 19.704072345156586 44-45 19.771782889329735 26.370093405868534 34.13861991374768 19.719503791054045 46-47 20.098158513831425 26.94680233010727 33.32407386921867 19.63096528684263 48-49 20.237378244192556 27.244211400940532 32.579916140393145 19.93849421447377 50-51 20.269048889151104 27.656954062405507 32.23879092703624 19.835206121407143 52-53 20.439037900707408 28.073466063244602 31.42092622501481 20.066569811033183 54-55 19.96721494166128 27.88790804123002 31.71587703103887 20.428999986069833 56-57 20.462760156116204 28.023358432217034 31.22147900682824 20.29240240483852 58-59 20.538925394121303 27.655520074600144 31.564365976631738 20.241188554646815 60-61 20.522455019899656 27.484834554681232 32.14750736045455 19.845203064964565 62-63 20.2513084114447 27.656790178084893 32.16729639216862 19.92460501830178 64-65 20.18903236961159 27.847264729717878 31.844690107041046 20.11901279362949 66-67 20.16264699399282 27.936622655532368 31.52359975187914 20.377130598595674 68-69 20.397124485710517 27.863817046099843 31.51847836685997 20.220580101329674 70-71 20.514424688189596 28.10411243119931 31.33316617132631 20.048296709284784 72-73 20.460219949146698 27.800475756182742 31.3385333828264 20.400770911844166 74-75 20.248481406914117 28.28463101035503 30.941646529380773 20.525241053350083 76-77 20.173995983195304 27.908639407787618 31.374915702002586 20.542448907014496 78-79 20.24925985743703 27.962065696307604 31.160022386598197 20.62865205965717 80-81 20.21197617449747 28.250829869228504 31.08307869807018 20.454115258203846 82-83 20.234346864333414 28.30333083272662 31.00111810306788 20.46120419987209 84-85 19.908216398216773 28.20618820268107 30.941218261175603 20.944377137926555 86-87 19.765391400825813 28.12086960298204 30.973563000820242 21.14017599537191 88-89 19.887690075083604 27.934574101524696 30.812710540138138 21.365025283253562 90-91 19.791629532289186 27.624370969255395 31.189639990149402 21.394359508306014 92-93 19.817117515394195 27.604168160436398 31.417839113727126 21.16087521044228 94-95 19.508215930703155 27.687231690638846 31.37196578423154 21.432586594426457 96-97 19.39874120453337 27.50880263657095 31.466281210744583 21.626174948151096 98-99 19.448049926087627 27.754874775067112 31.00333179280936 21.793743506035902 100-101 19.166989704054043 27.630145821671885 31.37686046997637 21.826004004297694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 981.0 1 733.0 2 711.5 3 1038.0 4 809.5 5 651.5 6 1495.5 7 3851.5 8 6547.0 9 7383.5 10 5920.0 11 4333.0 12 4122.5 13 4365.0 14 4530.5 15 4654.0 16 4828.5 17 4999.0 18 5102.0 19 5263.5 20 5620.0 21 6063.0 22 6714.0 23 7611.5 24 8654.5 25 10136.5 26 11869.5 27 13674.0 28 15278.0 29 16806.5 30 19427.0 31 22183.0 32 24841.5 33 27455.0 34 30209.0 35 32697.5 36 35637.0 37 38572.5 38 39053.5 39 38942.5 40 39168.0 41 39531.5 42 39857.0 43 40660.0 44 41847.5 45 42348.5 46 42424.5 47 44035.0 48 49758.0 49 47465.5 50 38692.5 51 34321.0 52 30784.5 53 29060.5 54 27671.5 55 25053.0 56 22390.0 57 19668.0 58 17341.0 59 15338.0 60 12706.0 61 9973.0 62 7783.5 63 5600.0 64 3690.5 65 2585.0 66 1875.0 67 1388.5 68 998.5 69 677.0 70 481.0 71 361.0 72 308.0 73 292.0 74 253.5 75 221.5 76 153.5 77 104.5 78 85.0 79 52.5 80 31.5 81 20.0 82 12.5 83 8.0 84 6.5 85 4.0 86 1.5 87 1.0 88 2.0 89 2.0 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035562897573119034 2 0.0 3 0.0 4 0.0 5 0.007456736587912056 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00569498014131745 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.458264809201777E-4 22-23 0.0014749588855210662 24-25 4.097108015336295E-5 26-27 0.0010242770038340738 28-29 0.001556901045827792 30-31 4.097108015336295E-5 32-33 0.0 34-35 0.0 36-37 2.8679756107354064E-4 38-39 4.097108015336295E-5 40-41 2.458264809201777E-4 42-43 0.022738949485116434 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0485540076681474E-4 84-85 2.0485540076681474E-4 86-87 0.0 88-89 0.0 90-91 0.011676757843708441 92-93 0.002171467248128236 94-95 0.0 96-97 0.0 98-99 7.374794427605331E-4 100-101 0.015077357496437566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1220373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.553027056041486 #Duplication Level Percentage of deduplicated Percentage of total 1 74.51172191525137 36.92281372058691 2 13.708728187283494 13.586179575367552 3 4.857714769177976 7.221434141628256 4 2.252962040096502 4.465643557165455 5 1.2576523940203488 3.116024155399285 6 0.7396575037460335 2.1991360977198786 7 0.5023231415304752 1.7424142556194775 8 0.3395555445964818 1.346080407073469 9 0.2666519796168639 1.189207148445133 >10 1.3933753569481633 12.656902914664023 >50 0.10729846566929008 3.644018926483772 >100 0.05597156111086552 5.094187612968274 >500 0.004370149072567236 1.5805951710596098 >1k 0.0015127439097348126 1.486977161243991 >5k 0.0 0.0 >10k+ 5.042479699116041E-4 3.7483851545748377 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18735 1.5351863733465096 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15272 1.251420672204318 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10997 0.9011179368930646 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3476 0.2848309492261792 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2982 0.24435152203465663 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2961 0.24263073666821539 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1737 0.14233353245278288 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1579 0.12938667112432017 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1472 0.12061885997150053 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1333 0.10922889968886561 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1243 0.10185410526126028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.19421603067259E-5 0.0 0.0 0.0 0.0 8 8.19421603067259E-5 0.0 0.0 0.0 0.0 9 8.19421603067259E-5 0.0 0.0 0.0 0.0 10-11 8.19421603067259E-5 0.0 0.0 0.0 0.0 12-13 1.638843206134518E-4 0.0 0.0 0.0 0.0 14-15 1.638843206134518E-4 0.0 0.0 0.0 0.0 16-17 1.638843206134518E-4 0.0 0.0 0.0 0.0 18-19 1.638843206134518E-4 0.0 0.0 0.0 0.0 20-21 1.638843206134518E-4 0.0 0.0 0.0 0.0 22-23 1.638843206134518E-4 0.0 0.0 1.2291324046008885E-4 0.0 24-25 1.638843206134518E-4 0.0 0.0 4.097108015336295E-4 0.0 26-27 1.638843206134518E-4 0.0 0.0 7.78450522913896E-4 0.0 28-29 1.638843206134518E-4 0.0 0.0 0.001679814286287881 0.0 30-31 1.638843206134518E-4 0.0 0.0 0.004547789897023287 0.0 32-33 1.638843206134518E-4 0.0 0.0 0.008603926832206218 0.0 34-35 1.638843206134518E-4 0.0 0.0 0.011881613244475255 0.0 36-37 1.638843206134518E-4 0.0 0.0 0.01610163450027164 0.0 38-39 1.638843206134518E-4 0.0 0.0 0.021304961679748734 0.0 40-41 1.638843206134518E-4 0.0 0.0 0.026344404538612375 0.0 42-43 1.638843206134518E-4 0.0 0.0 0.03888155506554143 0.0 44-45 1.638843206134518E-4 0.0 0.0 0.05309851987875838 0.0 46-47 1.638843206134518E-4 0.0 0.0 0.06752034009274213 0.0 48-49 1.638843206134518E-4 0.0 0.0 0.08763714044804335 0.0 50-51 1.638843206134518E-4 0.0 0.0 0.11033511885300643 0.0 52-53 1.638843206134518E-4 0.0 0.0 0.16105731608286974 0.0 54-55 1.638843206134518E-4 0.0 0.0 0.20522414048819498 0.0 56-57 1.638843206134518E-4 0.0 0.0 0.28261851089789763 0.0 58-59 1.638843206134518E-4 0.0 0.0 0.3695591429833338 0.0 60-61 1.638843206134518E-4 0.0 0.0 0.46244058169100755 0.0 62-63 1.638843206134518E-4 0.0 0.0 0.5903932650099601 0.0 64-65 1.638843206134518E-4 0.0 0.0 0.6554143692133471 0.0 66-67 1.638843206134518E-4 0.0 0.0 0.8067615392998697 0.0 68-69 1.638843206134518E-4 0.0 0.0 1.0027262156734047 0.0 70-71 1.638843206134518E-4 0.0 0.0 1.1553434892446819 0.0 72-73 1.638843206134518E-4 0.0 0.0 1.3992443293976513 0.0 74-75 1.638843206134518E-4 0.0 0.0 1.645521492199516 0.0 76-77 1.638843206134518E-4 0.0 0.0 1.813052238946617 0.0 78-79 1.638843206134518E-4 0.0 0.0 2.000986583610093 0.0 80-81 1.638843206134518E-4 0.0 0.0 2.3243303481804336 0.0 82-83 1.638843206134518E-4 0.0 0.0 2.5955998698758496 0.0 84-85 2.0485540076681474E-4 0.0 0.0 2.967617277668385 0.0 86-87 2.458264809201777E-4 0.0 0.0 3.4535342882872695 0.0 88-89 2.458264809201777E-4 0.0 0.0 4.069042825431241 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10330 0.0 33.91 1 GTATCAA 15340 0.0 30.921875 1 GTACATG 8075 0.0 25.486252 1 TATCAAC 19175 0.0 24.673761 2 ATCAACG 19170 0.0 24.655416 3 TCAACGC 19285 0.0 24.533024 4 TACATGG 8300 0.0 24.494982 2 GAGTACT 12645 0.0 24.477125 12-13 CAACGCA 19400 0.0 24.43657 5 AACGCAG 19765 0.0 23.958319 6 TAGGACG 100 0.0016533094 23.750973 4 ACATGGG 8320 0.0 23.294224 3 GTACTTT 13420 0.0 22.898645 14-15 AGTACTT 13095 0.0 22.729002 12-13 ACACCGT 170 3.15149E-6 22.351109 6 ACGCAGA 21795 0.0 21.688574 7 CATGGGG 1995 0.0 21.667553 4 CGCAGAG 21810 0.0 21.651875 8 CGGCTCG 55 0.0028779397 21.591352 90-91 AGAGTAC 18240 0.0 21.214415 10-11 >>END_MODULE