FastQCFastQC Report
Thu 26 May 2016
SRR937592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971072
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98201.011253542476768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.7811985105120939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.5388889804257563No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25310.26063978778092667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.18330257694589072No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13560.1396394912014763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.13830076451591644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12780.13160713108811706No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12700.13078329928161866No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12660.13037138337836948No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10210.10514153430435642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG951.9261279E-840.0040669
GTATCAA113450.029.8862441
AACCGAT700.00838466327.1470177
GGTATCA77800.025.7940271
GTACATG76350.025.3496341
TACATGG76500.024.9762132
ATCAACG136500.024.652593
TATCAAC137400.024.5948872
TCAACGC136850.024.5213454
CAACGCA137650.024.34435
AACGCAG140900.023.7717256
GAGTACT68600.023.64487812-13
ACATGGG77250.023.564723
AGTACTT71350.023.53238312-13
GTACTTT74250.021.94159314-15
CATGGGA57000.021.6811814
ACGCAGA154300.021.5214447
CGCAGAG154400.021.4767368
GGACCGA1553.8994393E-521.4560096
GTGTAGG4100.020.877331
Produced by FastQC (version 0.11.5)