##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937592_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 971072 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911380412574967 33.0 31.0 34.0 30.0 34.0 2 31.975616638107166 33.0 31.0 34.0 30.0 34.0 3 32.002286133263034 33.0 31.0 34.0 30.0 34.0 4 35.62990694819746 37.0 35.0 37.0 35.0 37.0 5 35.650057874184405 37.0 35.0 37.0 35.0 37.0 6 35.74831423416595 37.0 35.0 37.0 35.0 37.0 7 35.68217392737099 37.0 35.0 37.0 35.0 37.0 8 35.69488668193502 37.0 35.0 37.0 35.0 37.0 9 37.252749538654186 39.0 37.0 39.0 34.0 39.0 10-11 37.201656519804914 39.0 37.0 39.0 34.0 39.0 12-13 37.1104619430897 39.0 37.0 39.0 34.0 39.0 14-15 38.20140010215515 40.0 38.0 41.0 33.5 41.0 16-17 38.1453316540895 40.0 38.0 41.0 33.0 41.0 18-19 38.175838145884136 40.0 38.0 41.0 33.5 41.0 20-21 38.24086370526594 40.0 38.0 41.0 34.0 41.0 22-23 38.23518029559085 40.0 38.0 41.0 34.0 41.0 24-25 38.16205955892045 40.0 38.0 41.0 34.0 41.0 26-27 38.047924870658406 40.0 38.0 41.0 33.5 41.0 28-29 37.90660733704607 40.0 38.0 41.0 33.0 41.0 30-31 37.61724722780597 40.0 37.0 41.0 33.0 41.0 32-33 37.401301860212214 40.0 36.0 41.0 33.0 41.0 34-35 37.32643357032228 40.0 36.0 41.0 33.0 41.0 36-37 37.14097461362288 40.0 36.0 41.0 32.0 41.0 38-39 36.94937399080604 40.0 36.0 41.0 31.5 41.0 40-41 36.68730022078692 39.5 35.5 41.0 31.0 41.0 42-43 36.357967792295526 39.0 35.0 41.0 30.0 41.0 44-45 35.96328542064852 39.0 35.0 41.0 29.5 41.0 46-47 35.411452497858036 39.0 35.0 40.5 26.5 41.0 48-49 34.89635320552956 39.0 35.0 40.0 24.0 41.0 50-51 33.96677486324392 38.0 34.0 39.5 22.5 40.5 52-53 34.04263535556581 38.0 34.0 39.5 22.0 40.5 54-55 34.30470500642589 38.0 34.0 40.0 21.5 41.0 56-57 34.074414667501486 38.0 34.0 40.0 19.5 41.0 58-59 33.8123156676333 38.0 34.0 40.0 18.0 41.0 60-61 33.469963092335064 38.0 33.5 40.0 14.5 41.0 62-63 33.12529863902985 37.5 33.0 40.0 10.5 41.0 64-65 32.70397148718118 37.0 32.5 39.5 8.0 41.0 66-67 32.203813929348186 36.0 32.0 39.0 4.5 41.0 68-69 31.75058080142358 35.5 31.0 39.0 2.0 40.0 70-71 31.284934587754563 35.0 31.0 38.5 2.0 40.0 72-73 30.810462560963554 35.0 30.5 38.0 2.0 40.0 74-75 30.287281478613323 35.0 30.0 37.0 2.0 39.0 76-77 29.760636183516773 34.0 29.0 36.5 2.0 39.0 78-79 29.257604482468857 34.0 29.0 36.0 2.0 38.5 80-81 28.736763082449087 34.0 28.5 35.5 2.0 37.5 82-83 28.209942723093654 34.0 27.5 35.0 2.0 37.0 84-85 27.681713611349107 34.0 26.0 35.0 2.0 36.5 86-87 27.12973445841297 33.0 26.0 35.0 2.0 36.0 88-89 26.602847677618136 33.0 25.0 35.0 2.0 35.5 90-91 26.036280008073554 32.5 23.5 35.0 2.0 35.0 92-93 25.402335254234494 32.0 20.5 34.5 2.0 35.0 94-95 24.656177399822052 31.5 16.5 34.0 2.0 35.0 96-97 23.818522725400385 31.0 3.5 34.0 2.0 35.0 98-99 22.693794589896527 31.0 2.0 34.0 2.0 35.0 100-101 19.522567842549265 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12050509979183488 1101 2 0.026110325415764635 1101 3 0.012233605762954625 1101 4 0.06308936697452339 1101 5 -0.021693618956199145 1101 6 0.03028802360736904 1101 7 0.08161392549685331 1101 8 0.08952036057944213 1101 9 0.2891536258080407 1101 10-11 0.18247502575924557 1101 12-13 0.1793651301618837 1101 14-15 0.28819883608726826 1101 16-17 0.2200926769577407 1101 18-19 0.21199983004777323 1101 20-21 0.3529653054028401 1101 22-23 0.23267321051603318 1101 24-25 0.15707669013692538 1101 26-27 0.34226006130131026 1101 28-29 0.27424021505966323 1101 30-31 0.21448155421551007 1101 32-33 0.32613472099811247 1101 34-35 0.44370692182916116 1101 36-37 0.36255347926426396 1101 38-39 0.23960215951856156 1101 40-41 0.048154566035215396 1101 42-43 0.03938215545102253 1101 44-45 0.1567546134145772 1101 46-47 0.09342147940710532 1101 48-49 0.14201603903195092 1101 50-51 0.17163439201129194 1101 52-53 0.3010965309371727 1101 54-55 0.387634730874133 1101 56-57 0.4061731128320716 1101 58-59 0.4292752720007158 1101 60-61 0.2754154813763421 1101 62-63 0.4474148349559357 1101 64-65 0.732473216457457 1101 66-67 0.5909899950227171 1101 68-69 0.6440211463882441 1101 70-71 0.6715368880699515 1101 72-73 0.560097954652857 1101 74-75 0.6163104066670044 1101 76-77 0.6707083396116715 1101 78-79 0.8107045955813277 1101 80-81 0.917249034765387 1101 82-83 0.9395761811691017 1101 84-85 1.193761348725193 1101 86-87 1.4408095505769083 1101 88-89 1.2449214247580436 1101 90-91 1.3446485565847688 1101 92-93 1.4618724546704485 1101 94-95 1.4898726597199712 1101 96-97 1.4769617022916144 1101 98-99 1.6940628277379055 1101 100-101 2.456777654617781 1102 1 0.23900052244614756 1102 2 0.18929078958807466 1102 3 0.23327602941020942 1102 4 0.20956852068386667 1102 5 0.212096085016654 1102 6 0.023671604084633202 1102 7 0.20057990365216938 1102 8 0.2850136664159706 1102 9 0.2734520752935623 1102 10-11 0.3862944361054659 1102 12-13 0.36447757246768475 1102 14-15 0.42614252218719173 1102 16-17 0.4592555791804003 1102 18-19 0.38805169208362855 1102 20-21 0.4025223012674104 1102 22-23 0.3665503423370424 1102 24-25 0.2318622804999464 1102 26-27 0.2355169327755462 1102 28-29 0.2720586106427163 1102 30-31 0.425673000744041 1102 32-33 0.3962174842091244 1102 34-35 0.41829283298827846 1102 36-37 0.4661050139571614 1102 38-39 0.6273985412877394 1102 40-41 0.6959478653248894 1102 42-43 0.42883161915413837 1102 44-45 0.45188156792481493 1102 46-47 0.46984951305795875 1102 48-49 0.41902183300229723 1102 50-51 0.2947814944805458 1102 52-53 0.5800186451362208 1102 54-55 0.5710838617785825 1102 56-57 0.7062245329549341 1102 58-59 0.6031584869450128 1102 60-61 0.7465145080766149 1102 62-63 0.7366305212304454 1102 64-65 0.7728209637923413 1102 66-67 1.0147115843818924 1102 68-69 0.7548240095479777 1102 70-71 0.9210888373972601 1102 72-73 0.9854692161235015 1102 74-75 1.068240093399794 1102 76-77 0.9272054153479203 1102 78-79 1.193498085821055 1102 80-81 1.2947648017844955 1102 82-83 1.3435105707290447 1102 84-85 1.379078380379255 1102 86-87 1.3254061549393015 1102 88-89 1.5342169008783735 1102 90-91 1.711199698466114 1102 92-93 1.8014457631257201 1102 94-95 1.8301640767876322 1102 96-97 1.7233234217150546 1102 98-99 2.0583573491079434 1102 100-101 2.5621357132558167 1103 1 0.14953634699391216 1103 2 0.024314058963977203 1103 3 0.17626462913120733 1103 4 0.23573799263483863 1103 5 0.0764519177973213 1103 6 0.1066881041875476 1103 7 0.13730378134787458 1103 8 0.16786779544380437 1103 9 0.21172390962932752 1103 10-11 0.2880742289108227 1103 12-13 0.3348848723053166 1103 14-15 0.2688484018496453 1103 16-17 0.2328679925634347 1103 18-19 0.19724638300685626 1103 20-21 0.204443168237475 1103 22-23 0.08121605231028894 1103 24-25 0.1749973858124534 1103 26-27 0.1862674702009386 1103 28-29 0.15347627021231602 1103 30-31 0.20662498693106102 1103 32-33 0.06950644183255861 1103 34-35 0.0058124147112437186 1103 36-37 0.18315626375705563 1103 38-39 0.3373196140802932 1103 40-41 0.31554645429736894 1103 42-43 0.29746255656123566 1103 44-45 0.436538501855793 1103 46-47 0.12690422181913874 1103 48-49 0.22380491896748822 1103 50-51 0.19665061996365552 1103 52-53 0.2525693575107226 1103 54-55 0.16457872765092674 1103 56-57 0.13394081808768732 1103 58-59 0.31650779057891754 1103 60-61 0.5531121193922814 1103 62-63 0.6552949047920933 1103 64-65 0.565977223082939 1103 66-67 0.6248952237808894 1103 68-69 0.9434660385003362 1103 70-71 0.8996039710675312 1103 72-73 0.641674192453916 1103 74-75 0.7050241455872275 1103 76-77 0.8679842819520793 1103 78-79 0.7542257254944786 1103 80-81 0.8699221702296214 1103 82-83 0.9215687498860845 1103 84-85 1.2794617247660227 1103 86-87 1.1491149046820226 1103 88-89 1.2517791335950008 1103 90-91 1.4514116956303376 1103 92-93 1.7680267657099336 1103 94-95 2.0356270916297206 1103 96-97 2.000931507108522 1103 98-99 2.4200174790048017 1103 100-101 2.9403874227259372 1104 1 0.18177607894120484 1104 2 0.14079163441300224 1104 3 0.16306104996449378 1104 4 0.09424739342336608 1104 5 -0.010079883862616157 1104 6 0.14241243257579583 1104 7 0.14497152148829429 1104 8 0.10771947254921344 1104 9 0.33977191403372586 1104 10-11 0.3708357993397797 1104 12-13 0.12683322716732448 1104 14-15 0.27703561251492204 1104 16-17 0.38062553746581784 1104 18-19 0.20746152668264273 1104 20-21 0.22921162646079551 1104 22-23 0.1130761711817243 1104 24-25 -0.022391423815655287 1104 26-27 0.18070684162680095 1104 28-29 0.13146019178558532 1104 30-31 0.22132925872514875 1104 32-33 0.2842745744618185 1104 34-35 0.34612739736596154 1104 36-37 0.30556483102478893 1104 38-39 0.5377327485447196 1104 40-41 0.501690799024523 1104 42-43 0.49146481795998653 1104 44-45 0.7766655279388459 1104 46-47 0.6537024630192221 1104 48-49 0.8844915080441069 1104 50-51 0.8554328537757172 1104 52-53 0.8331908869740232 1104 54-55 0.8246644476873897 1104 56-57 0.7522912993749102 1104 58-59 0.8031835936122746 1104 60-61 0.8285613651980412 1104 62-63 1.0406495359236132 1104 64-65 0.9544016882206918 1104 66-67 1.03652011574286 1104 68-69 1.1770708975477113 1104 70-71 1.2928731278381171 1104 72-73 1.2258565369679495 1104 74-75 1.3290817727761102 1104 76-77 1.2196576874291623 1104 78-79 1.2849018659616789 1104 80-81 1.6573155381745863 1104 82-83 1.4480928260869348 1104 84-85 1.7789503940926892 1104 86-87 1.8196085348782063 1104 88-89 1.8964934840052514 1104 90-91 1.9166382498979893 1104 92-93 1.6971856378521082 1104 94-95 1.6849670976680144 1104 96-97 2.1152161325569523 1104 98-99 2.4735310829166686 1104 100-101 2.756048645065242 1105 1 0.05547242005327391 1105 2 0.05947478230963199 1105 3 -0.04178911443623434 1105 4 -0.13733858198283144 1105 5 -0.05497248605859539 1105 6 0.08362602547477849 1105 7 0.12444772568359497 1105 8 0.10726857097471765 1105 9 0.16033320875232704 1105 10-11 0.1415514341003572 1105 12-13 0.06185812736076457 1105 14-15 0.2183809089163944 1105 16-17 0.13994561813421313 1105 18-19 0.2595955349964143 1105 20-21 0.3302762297725863 1105 22-23 0.32688811870153245 1105 24-25 0.24262757715839456 1105 26-27 0.21116373388144893 1105 28-29 0.23092789012969206 1105 30-31 0.21637620096309718 1105 32-33 0.51510919967847 1105 34-35 0.2611283348388085 1105 36-37 0.37510016460507245 1105 38-39 0.3306619167547922 1105 40-41 0.45597825106478496 1105 42-43 0.4746840539882129 1105 44-45 0.7360107603177823 1105 46-47 0.291429571532845 1105 48-49 0.48666278661988116 1105 50-51 0.6034387804333292 1105 52-53 0.49934583065706306 1105 54-55 0.8048266076248254 1105 56-57 0.8614757894582752 1105 58-59 0.9036372390097398 1105 60-61 1.1040919519645769 1105 62-63 1.3649373995373182 1105 64-65 1.0613870041947777 1105 66-67 1.17013331901898 1105 68-69 1.2661811214893994 1105 70-71 1.2255557178240757 1105 72-73 1.324259174696433 1105 74-75 1.4774370795583813 1105 76-77 1.3492836586281705 1105 78-79 1.7468935155937793 1105 80-81 1.767579722596075 1105 82-83 1.7790486368180254 1105 84-85 1.8389683204027527 1105 86-87 1.8292238051676364 1105 88-89 1.7928945028781769 1105 90-91 2.0137078469675878 1105 92-93 2.21023068685221 1105 94-95 2.2937669952319553 1105 96-97 2.229665561613121 1105 98-99 2.007617138351037 1105 100-101 2.5466469356635315 1106 1 -0.31385125144431214 1106 2 -0.25983738700253767 1106 3 -0.2400752888093649 1106 4 -0.0754798288197307 1106 5 -0.26576153597807917 1106 6 -0.2961186260089974 1106 7 -0.25615958970632846 1106 8 -0.29130515803379353 1106 9 -0.1503178149421771 1106 10-11 -0.4257766082276859 1106 12-13 -0.44485339782902145 1106 14-15 -0.5938344119945782 1106 16-17 -0.41899157993341873 1106 18-19 -0.17710218757086693 1106 20-21 -0.24308470880573907 1106 22-23 -0.2256423620898218 1106 24-25 -0.45009153947376745 1106 26-27 -0.4166025417109154 1106 28-29 -0.0738167613540881 1106 30-31 -0.04811080156738967 1106 32-33 -0.11692438670294081 1106 34-35 -0.1496434618025546 1106 36-37 -0.19650093960473214 1106 38-39 -0.3220986077448629 1106 40-41 0.029310434179571132 1106 42-43 -0.0670401236055298 1106 44-45 0.021702041661242788 1106 46-47 0.17426831089332495 1106 48-49 0.3534042174917431 1106 50-51 0.47440151335258207 1106 52-53 0.5804017312781795 1106 54-55 0.5787223978249685 1106 56-57 0.45247532937086277 1106 58-59 0.25436762778360844 1106 60-61 0.7217248022061256 1106 62-63 0.6551087821000081 1106 64-65 0.699971085170155 1106 66-67 0.7982044025248527 1106 68-69 0.6500953151861957 1106 70-71 0.7737498747138822 1106 72-73 0.8548549879009393 1106 74-75 0.9896408982839411 1106 76-77 1.2815530398105928 1106 78-79 1.100062367675676 1106 80-81 0.9411016917702213 1106 82-83 0.8156773462728211 1106 84-85 1.0871969838488056 1106 86-87 1.1931184451492314 1106 88-89 1.3885524278839299 1106 90-91 1.7514580195003688 1106 92-93 1.816447618069141 1106 94-95 1.9478330177762402 1106 96-97 1.8189800313623756 1106 98-99 1.9645587072491288 1106 100-101 2.3823957279340675 1107 1 -0.16144300283229995 1107 2 0.07671702044739348 1107 3 -0.07455811436941318 1107 4 0.0634936427299877 1107 5 0.11988825298124794 1107 6 0.09749441247749502 1107 7 0.05410347623487155 1107 8 0.10819677488799329 1107 9 0.1567200170794365 1107 10-11 0.10310143744140277 1107 12-13 0.14220365248240796 1107 14-15 0.10152380250705306 1107 16-17 -0.013058591586414536 1107 18-19 0.3535160423497601 1107 20-21 0.22251649365464488 1107 22-23 0.10909005164973706 1107 24-25 0.13742100926526035 1107 26-27 0.20267564927396364 1107 28-29 0.22608215543321108 1107 30-31 0.3654616649739353 1107 32-33 0.2665692377056743 1107 34-35 0.22693726333430675 1107 36-37 0.40235400529174115 1107 38-39 0.350759232374493 1107 40-41 0.2081563107055331 1107 42-43 0.35881346796837477 1107 44-45 0.30821263251965547 1107 46-47 0.6087070425416599 1107 48-49 0.36868248057987074 1107 50-51 0.17563822278201968 1107 52-53 0.17453945958203576 1107 54-55 0.36310663605411264 1107 56-57 0.46534229332478105 1107 58-59 0.11015349776480576 1107 60-61 0.37832335903181047 1107 62-63 0.7248624385668379 1107 64-65 0.5240620295563687 1107 66-67 0.6975129046075281 1107 68-69 0.5468899096608659 1107 70-71 0.8127327751503906 1107 72-73 0.7918145601622619 1107 74-75 0.8249279524223887 1107 76-77 0.8231226444074515 1107 78-79 0.7735046601153712 1107 80-81 1.1281097950664467 1107 82-83 1.231655565544358 1107 84-85 1.2467711864145734 1107 86-87 1.3805161833256854 1107 88-89 1.1108280342445482 1107 90-91 1.2966612120105054 1107 92-93 1.340924592172982 1107 94-95 1.387214288679246 1107 96-97 1.696841282520186 1107 98-99 1.8536701455980769 1107 100-101 2.870285154824142 1108 1 0.0774185898255304 1108 2 -0.007697980515303016 1108 3 -0.018922769830759023 1108 4 0.014306230531545339 1108 5 -0.02400860292079443 1108 6 0.0656826162270221 1108 7 -0.0033645586504249536 1108 8 -0.05265781503862854 1108 9 0.09743925890185778 1108 10-11 0.06934071406355002 1108 12-13 0.034114856139225935 1108 14-15 0.03356379366450568 1108 16-17 0.044086404883692865 1108 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2316 68-69 -1.5485658599242527 2316 70-71 -1.6364033108016187 2316 72-73 -2.02569334192275 2316 74-75 -1.840172471384502 2316 76-77 -1.9610405850294086 2316 78-79 -2.0221608867939587 2316 80-81 -1.938104716421698 2316 82-83 -1.9659249081555181 2316 84-85 -2.0834438353427345 2316 86-87 -2.536628860685031 2316 88-89 -2.561182284531938 2316 90-91 -2.4537321204723774 2316 92-93 -2.7290177190628597 2316 94-95 -2.5691010009693755 2316 96-97 -2.283222784608558 2316 98-99 -2.3761706787701122 2316 100-101 -2.4486047262547963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3594.0 3 579.0 4 527.0 5 606.0 6 705.0 7 814.0 8 1043.0 9 1312.0 10 1735.0 11 2413.0 12 3585.0 13 5229.0 14 6115.0 15 8495.0 16 11856.0 17 16812.0 18 13952.0 19 7936.0 20 7212.0 21 7275.0 22 7667.0 23 8403.0 24 9512.0 25 10535.0 26 12015.0 27 14123.0 28 16695.0 29 19868.0 30 24055.0 31 29714.0 32 38141.0 33 49567.0 34 68453.0 35 97439.0 36 138340.0 37 183830.0 38 129354.0 39 11566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59290679619875 20.0892151525263 15.161204011995904 20.156674039279046 2 14.747430733313577 21.37997474358961 41.73565025963713 22.136944263459675 3 19.176231996596545 27.36610259100315 30.597525061233256 22.860140351167047 4 11.52708769582151 17.45656097571049 38.751388768425706 32.26496256004229 5 11.884007690417334 39.81546302548948 33.76452536867126 14.53600391542193 6 28.804214349840567 38.287842983479 17.844118587924505 15.063824078755921 7 26.42788525532352 35.905846711991934 20.517611952002955 17.148656080681594 8 26.185744239992736 35.03584784169989 19.410254505229616 19.368153413077753 9 27.148634410965578 17.22766903079141 21.07992310145098 34.54377345679203 10-11 24.318375307648253 28.584121480426607 26.38623593331579 20.711267278609352 12-13 26.02113818872874 26.463654193556373 27.635784973134797 19.87942264458008 14-15 22.697898129692987 27.021548079051417 27.883202941390444 22.397350849865152 16-17 19.821759406153603 30.93682591618647 28.126033455451978 21.115381222207947 18-19 19.697710698676442 28.434983759868928 32.461587034308565 19.40571850714607 20-21 21.263686489118903 26.968213090329797 32.54835927214498 19.21974114840632 22-23 20.618356181910862 27.230444912674617 32.54952359529229 19.601675310122232 24-25 20.14660254181237 27.510883236977516 32.844933348879586 19.497580872330524 26-27 19.971396244054425 27.540556813591117 32.99822980723937 19.489817135115082 28-29 19.531299984933572 28.089133391535142 32.90306302513204 19.476503598399244 30-31 19.715122470713524 27.838197901445543 32.81344164582147 19.633237982019466 32-33 19.510682440984333 27.983986270998155 32.60834007967651 19.896991208341007 34-35 20.128157037001877 27.91907087954074 32.4890317327721 19.46374035068529 36-37 20.050383915530844 27.58052475420687 32.66911449468792 19.69997683557437 38-39 19.870926471422752 28.188327667235857 32.56527189529671 19.375473966044684 40-41 19.879991663795078 28.270877137958 32.37258915509262 19.476542043154303 42-43 19.58270071384757 28.494935690779204 31.818657851007732 20.103705744365495 44-45 19.958738400011555 28.579565035256536 31.45982901044386 20.00186755428805 46-47 20.273321958676664 28.976172553576024 30.73453212652594 20.015973361221366 48-49 20.28700821387127 29.277995606953954 30.290759381918956 20.144236797255815 50-51 20.243816741195463 29.68702032889669 30.056336443988346 20.0128264859195 52-53 20.339987048603856 30.00281215573667 29.59600415889454 20.06119663676494 54-55 20.024352617648425 29.723195352364833 29.7837673293124 20.46868470067434 56-57 20.355727453408846 30.096011645403248 29.294564842292374 20.253696058895535 58-59 20.518255606338638 29.595928964200507 29.58659409190259 20.299221337558265 60-61 20.313937083624563 29.657666208433387 30.097518576502175 19.930878131439876 62-63 20.289757892054663 29.64236233972689 30.120615539515207 19.947264228703236 64-65 20.12422276946313 29.841680705906388 29.93355046440395 20.10054606022653 66-67 20.06792509669319 29.801104504989667 29.573415124063374 20.55755527425377 68-69 20.054667020177998 29.910824358856647 29.63016455815038 20.404344062814975 70-71 20.177239022503063 30.013334193049197 29.4162615255658 20.39316525888194 72-73 20.122314220124327 29.81897908122469 29.523691609275453 20.535015089375534 74-75 19.883952666885754 30.387593139927986 29.223456893941073 20.504997299245183 76-77 20.13690842230816 29.97070743756629 29.30396373046303 20.588420409662522 78-79 20.08683320358909 29.981392147971377 29.18322001992335 20.748554628516185 80-81 19.969459899351797 30.290775806256075 29.09785471269349 20.641909581698638 82-83 20.094211929384382 30.263261061545194 29.106296889314393 20.53623011975603 84-85 19.94614989825735 30.123819127618034 28.927304941675285 21.002726032449328 86-87 19.947767183802153 30.09968905844752 28.9943196941342 20.958224063616125 88-89 20.077668220099852 29.941212338186908 28.788002282174585 21.193117159538655 90-91 19.880962145281273 29.607854971491477 29.424469820891257 21.086713062335992 92-93 20.06181787044039 29.803445582838734 29.266614326435906 20.868122220284967 94-95 19.68514050755734 29.738844793015918 29.26303235514261 21.312982344284137 96-97 19.54972318117756 29.647832394095907 29.331961792192946 21.47048263253359 98-99 19.793506078797645 30.126559292716532 28.57537868998003 21.504555938505792 100-101 19.896586583592082 29.457917702520092 29.171454802405588 21.47404091148224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1944.0 1 1183.0 2 497.5 3 612.5 4 617.5 5 692.5 6 1133.0 7 2173.5 8 3427.0 9 3927.0 10 3532.0 11 3129.5 12 3115.5 13 3255.5 14 3420.0 15 3418.5 16 3409.0 17 3483.5 18 3630.0 19 3828.5 20 4118.5 21 4635.5 22 5271.5 23 6114.0 24 7020.0 25 8245.5 26 9888.5 27 11619.5 28 12886.5 29 14234.5 30 16349.0 31 18427.5 32 20591.5 33 22792.0 34 25206.0 35 26937.0 36 29126.0 37 31514.0 38 31789.0 39 31378.0 40 31383.5 41 31776.5 42 31994.0 43 32657.5 44 33455.5 45 33677.5 46 33385.5 47 34345.0 48 38795.0 49 37484.5 50 30872.0 51 27036.0 52 24243.0 53 22838.5 54 21677.5 55 19635.5 56 17640.5 57 15663.0 58 13586.0 59 11835.0 60 10053.0 61 8092.5 62 6275.5 63 4447.0 64 2906.5 65 2028.5 66 1476.5 67 1075.5 68 765.5 69 536.5 70 392.0 71 305.0 72 254.0 73 219.5 74 184.0 75 148.5 76 111.5 77 70.0 78 44.5 79 31.5 80 15.5 81 11.0 82 8.5 83 6.5 84 4.0 85 3.0 86 3.5 87 2.0 88 1.5 89 2.0 90 2.5 91 2.5 92 2.0 93 1.0 94 1.0 95 1.5 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31666035062281683 2 0.2682602319910367 3 0.27227641204771635 4 0.26650958940222763 5 0.2671274632571014 6 0.2061639095762209 7 0.19864644434192316 8 0.20348645620510117 9 0.2062668885520332 10-11 0.20873838397152839 12-13 0.21290903249192644 14-15 0.20662731496737624 16-17 0.19900687075726622 18-19 0.19236472681737296 20-21 0.19236472681737296 22-23 0.19957325512423382 24-25 0.1978226125354248 26-27 0.20271411388650892 28-29 0.20904732089896527 30-31 0.2084294470440915 32-33 0.2088928524352468 34-35 0.20868689448362224 36-37 0.19705027021683255 38-39 0.19123195808343768 40-41 0.18598003031701046 42-43 0.19437281684571278 44-45 0.19463026428524352 46-47 0.20168432412838597 48-49 0.21012860014499438 50-51 0.20425879852369339 52-53 0.21260009556448956 54-55 0.20358943518091346 56-57 0.1815004448691755 58-59 0.16363359256574178 60-61 0.18222129769986162 62-63 0.17619702761484216 64-65 0.18175789230870626 66-67 0.18216980821195544 68-69 0.18011022869570947 70-71 0.1811915079417386 72-73 0.1902021683253147 74-75 0.1955055855796481 76-77 0.19401239043036975 78-79 0.19128344757134386 80-81 0.19097451064390694 82-83 0.18206682923614315 84-85 0.1765574540301852 86-87 0.18103703947802016 88-89 0.18752471495419495 90-91 0.18541364595004284 92-93 0.18258172411520465 94-95 0.1835600243854215 96-97 0.19009918934950243 98-99 0.18973876293415937 100-101 0.17115105780003956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 971072.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.818848298573 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22590702497519 44.447059442703406 2 12.600710483654588 14.319157148500167 3 4.002886893933864 6.8231826954832275 4 1.7599591490684567 3.9999540761042507 5 0.8728085026320278 2.479598695237692 6 0.5363520070439254 1.8284941993718373 7 0.34830549101336966 1.3853221798814044 8 0.2654199294023836 1.2064683763305595 9 0.19236795652610195 0.9837113165426764 >10 1.0724213135463372 11.180047095954631 >50 0.08185206835310978 3.1814676677515217 >100 0.03647276633887126 3.655155065662971 >500 0.00254039156658558 0.9550293469478093 >1k 0.0014516523237631887 1.2212374624416282 >5k 5.443696214111958E-4 2.3341152310861983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9820 1.011253542476768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7586 0.7811985105120939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5233 0.5388889804257563 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2531 0.26063978778092667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1780 0.18330257694589072 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1356 0.1396394912014763 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1343 0.13830076451591644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1278 0.13160713108811706 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1270 0.13078329928161866 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1266 0.13037138337836948 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1021 0.10514153430435642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0297897581229816E-4 0.0 8 0.0 0.0 0.0 1.0297897581229816E-4 0.0 9 0.0 0.0 0.0 1.0297897581229816E-4 0.0 10-11 0.0 0.0 0.0 1.0297897581229816E-4 0.0 12-13 0.0 0.0 0.0 1.0297897581229816E-4 0.0 14-15 0.0 0.0 0.0 1.0297897581229816E-4 0.0 16-17 0.0 0.0 0.0 3.089369274368945E-4 0.0 18-19 0.0 0.0 0.0 3.6042641534304355E-4 0.0 20-21 0.0 0.0 0.0 4.119159032491926E-4 0.0 22-23 0.0 0.0 0.0 7.208528306860871E-4 0.0 24-25 0.0 0.0 0.0 9.783002702168325E-4 0.0 26-27 0.0 0.0 0.0 0.0015961741250906216 0.0 28-29 0.0 0.0 0.0 0.0030378797864627958 0.0 30-31 0.0 0.0 0.0 0.005509375205957952 0.0 32-33 0.0 0.0 0.0 0.00895917089566994 0.0 34-35 0.0 0.0 0.0 0.01235747709747578 0.0 36-37 0.0 0.0 0.0 0.017197488960653793 0.0 38-39 0.0 0.0 0.0 0.022603885190799446 0.0 40-41 0.0 0.0 0.0 0.02795879193303895 0.0 42-43 0.0 0.0 0.0 0.04031626903051473 0.0 44-45 0.0 0.0 0.0 0.05447587820470573 0.0 46-47 0.0 0.0 0.0 0.06853250840308442 0.0 48-49 0.0 0.0 0.0 0.08881936663810716 0.0 50-51 0.0 0.0 0.0 0.11080537797403282 0.0 52-53 0.0 0.0 0.0 0.16450891386014632 0.0 54-55 0.0 0.0 0.0 0.20817199960456073 0.0 56-57 0.0 0.0 0.0 0.2927692282343637 0.0 58-59 0.0 0.0 0.0 0.39137159757463913 0.0 60-61 0.0 0.0 0.0 0.4999629275687075 0.0 62-63 0.0 0.0 0.0 0.6482526527384169 0.0 64-65 0.0 0.0 0.0 0.7243026263757991 0.0 66-67 0.0 0.0 0.0 0.8956596421274633 0.0 68-69 0.0 0.0 0.0 1.129010001318131 0.0 70-71 0.0 0.0 0.0 1.302787023001384 0.0 72-73 0.0 0.0 0.0 1.5854128221182364 0.0 74-75 0.0 0.0 0.0 1.8719003328280497 0.0 76-77 0.0 0.0 0.0 2.0580863210966847 0.0 78-79 0.0 0.0 0.0 2.2660008732617145 0.0 80-81 0.0 0.0 0.0 2.629156231463784 0.0 82-83 0.0 0.0 0.0 2.9344888947472487 0.0 84-85 0.0 0.0 0.0 3.368493788308179 0.0 86-87 0.0 0.0 0.0 3.937298161207408 0.0 88-89 0.0 0.0 0.0 4.672619537995123 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 95 1.9261279E-8 40.004066 9 GTATCAA 11345 0.0 29.886244 1 AACCGAT 70 0.008384663 27.147017 7 GGTATCA 7780 0.0 25.794027 1 GTACATG 7635 0.0 25.349634 1 TACATGG 7650 0.0 24.976213 2 ATCAACG 13650 0.0 24.65259 3 TATCAAC 13740 0.0 24.594887 2 TCAACGC 13685 0.0 24.521345 4 CAACGCA 13765 0.0 24.3443 5 AACGCAG 14090 0.0 23.771725 6 GAGTACT 6860 0.0 23.644878 12-13 ACATGGG 7725 0.0 23.56472 3 AGTACTT 7135 0.0 23.532383 12-13 GTACTTT 7425 0.0 21.941593 14-15 CATGGGA 5700 0.0 21.681181 4 ACGCAGA 15430 0.0 21.521444 7 CGCAGAG 15440 0.0 21.476736 8 GGACCGA 155 3.8994393E-5 21.456009 6 GTGTAGG 410 0.0 20.87733 1 >>END_MODULE