FastQCFastQC Report
Thu 26 May 2016
SRR937592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971072
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153141.5770200355895339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124481.2818822909114875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90530.9322686680287353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.2925632702827391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.25425509128056417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23000.23685164436828576No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16230.16713487774335992No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15420.15879358070256377No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.1232658340473209No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.11636624266789693No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.10905473538522376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85000.031.3032461
GTATCAA125700.030.9577161
CACGTCA700.00838743427.1451849
GTGTAAG4850.025.4713441
GTACATG61750.025.392021
TCAACGC156800.024.841414
TATCAAC156850.024.7729232
ATCAACG157300.024.7020513
TACATGG62000.024.6701972
CAACGCA159150.024.4746025
AACGCAG161750.024.0787126
GAGTACT102100.023.82182312-13
ATCGCGT500.00165471823.74836524-25
TCGCGAT500.00165471823.74836550-51
AGTACTT105050.023.24330512-13
ACATGGG63200.023.1495253
CGGGTCG1050.002196856822.617496
GTACTTT109300.022.14051414-15
CGCAGAG176100.022.0121068
ACGCAGA176750.022.0117867
Produced by FastQC (version 0.11.5)