##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 971072 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27329281453898 33.0 31.0 34.0 31.0 34.0 2 32.4620048770843 34.0 31.0 34.0 31.0 34.0 3 32.54133370131154 34.0 31.0 34.0 31.0 34.0 4 36.174256903710535 37.0 35.0 37.0 35.0 37.0 5 36.04657636097014 37.0 35.0 37.0 35.0 37.0 6 36.16335348975153 37.0 36.0 37.0 35.0 37.0 7 36.12707399657286 37.0 35.0 37.0 35.0 37.0 8 36.13691363771172 37.0 36.0 37.0 35.0 37.0 9 37.77292620938509 39.0 38.0 39.0 35.0 39.0 10-11 37.758748063995256 39.0 37.0 39.0 35.0 39.0 12-13 37.67617488713505 39.0 37.0 39.0 35.0 39.0 14-15 38.82324946039017 40.0 38.0 41.0 35.0 41.0 16-17 38.822058508534894 40.0 38.0 41.0 35.5 41.0 18-19 38.92953045706189 40.0 38.0 41.0 35.0 41.0 20-21 38.916172024319515 40.0 38.0 41.0 35.0 41.0 22-23 38.78413083684835 40.0 38.0 41.0 35.0 41.0 24-25 38.71825055196731 40.0 38.0 41.0 35.0 41.0 26-27 38.60881067521255 40.0 38.0 41.0 34.5 41.0 28-29 38.48161979750214 40.0 38.0 41.0 34.0 41.0 30-31 38.38908494941673 40.0 37.5 41.0 34.5 41.0 32-33 38.16635790137086 40.0 37.0 41.0 34.0 41.0 34-35 37.97046717442167 40.0 37.0 41.0 34.0 41.0 36-37 37.76859336897779 40.0 36.0 41.0 33.0 41.0 38-39 37.59408107740724 40.0 36.0 41.0 33.0 41.0 40-41 37.35897698625848 40.0 36.0 41.0 33.0 41.0 42-43 37.06122666496408 40.0 36.0 41.0 33.0 41.0 44-45 36.555196731035394 40.0 35.0 41.0 31.0 41.0 46-47 35.896052506920185 39.5 35.0 41.0 29.0 41.0 48-49 35.29828787154814 39.0 35.0 41.0 25.0 41.0 50-51 34.955888440815926 39.0 35.0 41.0 23.5 41.0 52-53 34.715585970968164 39.0 35.0 40.5 23.0 41.0 54-55 34.5152192628353 39.0 35.0 40.0 22.0 41.0 56-57 34.243149838528964 38.0 34.5 40.0 20.0 41.0 58-59 34.002120852006854 38.0 34.0 40.0 18.5 41.0 60-61 33.66226345729255 38.0 34.0 40.0 15.5 41.0 62-63 33.321877265537466 37.5 33.5 40.0 11.5 41.0 64-65 32.94351706155672 37.0 33.0 40.0 8.0 41.0 66-67 32.54931405704211 36.0 33.0 39.0 6.5 41.0 68-69 32.10470438855204 36.0 32.0 39.0 2.0 40.0 70-71 31.69070007167337 35.0 32.0 38.5 2.0 40.0 72-73 31.244790293613654 35.0 31.0 38.0 2.0 40.0 74-75 30.765908707078363 35.0 31.0 37.0 2.0 39.0 76-77 29.52988295409609 34.0 29.5 36.0 2.0 38.5 78-79 30.061756491794632 34.0 30.5 36.0 2.0 39.0 80-81 29.840284242569037 34.0 30.0 36.0 2.0 37.5 82-83 29.494281577473142 34.0 30.0 35.5 2.0 37.0 84-85 29.109182429315233 34.0 29.5 35.0 2.0 37.0 86-87 28.718234075331182 34.0 29.0 35.0 2.0 36.0 88-89 28.287743854214725 34.0 29.0 35.0 2.0 36.0 90-91 27.80056061754432 34.0 27.5 35.0 2.0 35.5 92-93 27.403877879292164 34.0 27.0 35.0 2.0 35.0 94-95 26.976780815428725 33.0 26.0 35.0 2.0 35.0 96-97 26.479717260924012 33.0 25.0 35.0 2.0 35.0 98-99 25.755856929249326 33.0 23.5 35.0 2.0 35.0 100-101 23.505124748731298 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13007478299776665 1101 2 0.11775714049152697 1101 3 0.15791003824696048 1101 4 0.06694681037083683 1101 5 -0.028562447478840625 1101 6 2.6786785390697787E-4 1101 7 0.035910081616144396 1101 8 0.11483063719906994 1101 9 0.20664448356005494 1101 10-11 0.21083007749935945 1101 12-13 0.22976478313810134 1101 14-15 0.32281652363438695 1101 16-17 0.10372780512063429 1101 18-19 0.1405842592901152 1101 20-21 0.17465786227927538 1101 22-23 0.03967837424566767 1101 24-25 0.013433698707679298 1101 26-27 0.0737345873112929 1101 28-29 0.04011617811704582 1101 30-31 0.1890744940549851 1101 32-33 0.19472566333476493 1101 34-35 0.14661261815359694 1101 36-37 0.1897545602260422 1101 38-39 0.2187534743520132 1101 40-41 0.12138118041811907 1101 42-43 0.02542270357061227 1101 44-45 0.29872902439389293 1101 46-47 0.2590577168015429 1101 48-49 0.3231909760377789 1101 50-51 0.4410838896774649 1101 52-53 0.3765722498260544 1101 54-55 0.4012181929108394 1101 56-57 0.390725566882395 1101 58-59 0.2949452326647233 1101 60-61 0.35531366901449246 1101 62-63 0.3403071073994255 1101 64-65 0.2589568173176531 1101 66-67 0.560717728428969 1101 68-69 0.45619579881127237 1101 70-71 0.38126026599831775 1101 72-73 0.41080755990402196 1101 74-75 0.25183056740831233 1101 76-77 0.2795651821220382 1101 78-79 0.25849588948076274 1101 80-81 0.26798881771618355 1101 82-83 0.2135641291000745 1101 84-85 0.4456853729562944 1101 86-87 0.5236749615912011 1101 88-89 0.6241525970360726 1101 90-91 0.49305702600444334 1101 92-93 0.4688219868119923 1101 94-95 0.5270415669130379 1101 96-97 0.548406656292979 1101 98-99 0.9460151831623556 1101 100-101 1.6278220520559579 1102 1 0.13826654050210863 1102 2 0.2026658118064688 1102 3 0.09783851381080666 1102 4 0.09615398572846345 1102 5 0.05757375992565983 1102 6 -0.008421723731196096 1102 7 0.0942112262253545 1102 8 0.10153778527811141 1102 9 0.2010864729323245 1102 10-11 0.22939110513802063 1102 12-13 0.20702672297247204 1102 14-15 0.1995923591772808 1102 16-17 0.21505100718943737 1102 18-19 0.26320693106334403 1102 20-21 0.23323656643761836 1102 22-23 0.2516868142382549 1102 24-25 0.23880114117715578 1102 26-27 0.11979787907207395 1102 28-29 0.16048495723692469 1102 30-31 0.17160529157154514 1102 32-33 0.14643302964244498 1102 34-35 0.1491577096459551 1102 36-37 0.17530850634538808 1102 38-39 0.17535461787008444 1102 40-41 0.12491716632055017 1102 42-43 -0.04041551262849907 1102 44-45 0.01570216590425133 1102 46-47 -0.03647432461825417 1102 48-49 -0.10027487663606394 1102 50-51 -0.1635976601823259 1102 52-53 -0.38672196602389164 1102 54-55 -0.24841436461969124 1102 56-57 -0.044559412440449364 1102 58-59 -0.09863625390876507 1102 60-61 0.0702300056103482 1102 62-63 0.12689859430025763 1102 64-65 0.01767232355353343 1102 66-67 0.17588107256461427 1102 68-69 0.2322311573063729 1102 70-71 0.20602062367505525 1102 72-73 0.054749123730445604 1102 74-75 0.1141194312009084 1102 76-77 -0.0559054279361213 1102 78-79 0.029060528803380947 1102 80-81 0.06484523418371779 1102 82-83 -0.054983941213190946 1102 84-85 0.09479827354224213 1102 86-87 0.1714137037213277 1102 88-89 0.21937734525478447 1102 90-91 0.19782163913962592 1102 92-93 0.34404498920159554 1102 94-95 0.31902671099849655 1102 96-97 0.497807618681275 1102 98-99 0.6454195553731203 1102 100-101 1.368502325278211 1103 1 0.16358270671520359 1103 2 0.18428614935535137 1103 3 0.10983053247204566 1103 4 0.00778753022042622 1103 5 0.055694200385786985 1103 6 0.06681926010030281 1103 7 0.07296577147199912 1103 8 0.047266396891075146 1103 9 0.1806014179319817 1103 10-11 0.1207425584734878 1103 12-13 0.07573913178167402 1103 14-15 0.07519196011034524 1103 16-17 -0.07743489968731865 1103 18-19 0.09565241669006497 1103 20-21 0.08363863495363688 1103 22-23 0.03948811549376785 1103 24-25 0.06600387061205737 1103 26-27 0.032714240816531515 1103 28-29 0.01908419226334246 1103 30-31 -0.05074946064104324 1103 32-33 0.07068535086274608 1103 34-35 0.0374432654810235 1103 36-37 -0.0536647252963931 1103 38-39 0.012630500048139481 1103 40-41 -0.16262757148446383 1103 42-43 -0.061114251394016605 1103 44-45 -0.15025365086798814 1103 46-47 0.10825247461334442 1103 48-49 0.14143790951436586 1103 50-51 0.06701000213157471 1103 52-53 0.1533147961596555 1103 54-55 0.046254118239872355 1103 56-57 0.3345348828462136 1103 58-59 0.25682633213646966 1103 60-61 0.21300292051418523 1103 62-63 0.1198727578605272 1103 64-65 0.18953195833296377 1103 66-67 0.17258148077921476 1103 68-69 0.08589351122957822 1103 70-71 0.2504489802968237 1103 72-73 0.2967978007492178 1103 74-75 0.1387916874727786 1103 76-77 0.06015487233602457 1103 78-79 0.015300437784102172 1103 80-81 -0.026811522511227537 1103 82-83 0.13504881886639453 1103 84-85 0.37665502108952253 1103 86-87 0.3150506442843657 1103 88-89 0.4068659106720318 1103 90-91 0.5638304837893813 1103 92-93 0.5659294941985067 1103 94-95 0.5497858815338681 1103 96-97 0.714351458871544 1103 98-99 1.015319731465695 1103 100-101 1.7863691466928984 1104 1 0.20724783026334848 1104 2 0.21709493092317445 1104 3 0.11596798052499224 1104 4 0.07596692662823301 1104 5 -0.02986525900967507 1104 6 0.041971253327943714 1104 7 -0.045414309275329856 1104 8 0.07045313396498898 1104 9 0.13430490238042125 1104 10-11 0.21233660779802932 1104 12-13 0.13593481381243322 1104 14-15 0.08660514193390867 1104 16-17 0.021617843590640007 1104 18-19 0.06505508478540634 1104 20-21 0.14968872020008206 1104 22-23 0.1761244032478828 1104 24-25 0.13092399646895814 1104 26-27 0.14769297360444256 1104 28-29 0.16113607123706686 1104 30-31 0.1718321894696544 1104 32-33 0.09513931482243976 1104 34-35 0.1229035666851317 1104 36-37 0.20067381556862784 1104 38-39 0.1173156505909958 1104 40-41 0.12634492765715066 1104 42-43 0.2561064924996117 1104 44-45 0.16428205200079304 1104 46-47 0.29545241008651857 1104 48-49 0.31077790075292455 1104 50-51 0.32520498265513 1104 52-53 0.4055073761670016 1104 54-55 0.3954370991422991 1104 56-57 0.5326681188959981 1104 58-59 0.4822595202351181 1104 60-61 0.38283759272975715 1104 62-63 0.5576979819284418 1104 64-65 0.47318987441257576 1104 66-67 0.512988221760267 1104 68-69 0.48452683015784004 1104 70-71 0.5078381155189966 1104 72-73 0.5353376297827381 1104 74-75 0.6647678036684184 1104 76-77 0.35650873684153694 1104 78-79 0.4742940857399631 1104 80-81 0.5521613170241757 1104 82-83 0.6978477734134607 1104 84-85 0.628176433338961 1104 86-87 0.7805028944353261 1104 88-89 0.8860138310139654 1104 90-91 0.7623255732389502 1104 92-93 0.962976282065096 1104 94-95 0.9643622096616404 1104 96-97 1.324106996930528 1104 98-99 1.686986240268574 1104 100-101 2.1663647955722602 1105 1 0.10394655392836682 1105 2 0.10713622545885215 1105 3 -0.0013258783419018982 1105 4 0.08417370112714195 1105 5 -0.0994074521473749 1105 6 -0.04150773127463481 1105 7 -0.01778584119742277 1105 8 -0.05216282685321261 1105 9 0.038320487294882355 1105 10-11 0.1298115589808404 1105 12-13 0.14203524687915348 1105 14-15 0.08709445702996987 1105 16-17 -0.05120138877914826 1105 18-19 0.05703306103304584 1105 20-21 -0.061464920167040304 1105 22-23 -0.0491443770770843 1105 24-25 0.007380166183558856 1105 26-27 -0.07889924399901105 1105 28-29 -0.2337828259290191 1105 30-31 -0.15180630270815954 1105 32-33 -0.04361078235461946 1105 34-35 -0.017035063141136675 1105 36-37 0.10296262122822242 1105 38-39 -0.0971895695322047 1105 40-41 -0.08344686190991979 1105 42-43 -0.025814457960663617 1105 44-45 -0.07414290215449881 1105 46-47 -0.23598585618320556 1105 48-49 -0.17630326610352398 1105 50-51 -0.31335121769881624 1105 52-53 -0.28781461603140457 1105 54-55 -0.28750729728523794 1105 56-57 -0.265075086712379 1105 58-59 -0.30385261980961076 1105 60-61 -0.19342193633875837 1105 62-63 -0.10511772920187923 1105 64-65 -0.024189774652469964 1105 66-67 -0.07872360365942654 1105 68-69 -0.05236707078100267 1105 70-71 -0.00917312958213401 1105 72-73 0.06167512915394724 1105 74-75 -0.01444316430659498 1105 76-77 -0.5417155523939883 1105 78-79 -0.42780585343862754 1105 80-81 -0.17141360992153665 1105 82-83 0.004872874820584627 1105 84-85 0.05715440501356284 1105 86-87 0.18708532573685588 1105 88-89 0.30262676448082715 1105 90-91 0.18623707580213988 1105 92-93 0.44528868460221815 1105 94-95 0.432157502176036 1105 96-97 0.3754997157389788 1105 98-99 0.5598457153752427 1105 100-101 1.0027286813743501 1106 1 -0.20993658386781533 1106 2 0.005686950096531973 1106 3 -0.1214662050039621 1106 4 -0.0052327861054308755 1106 5 -0.1361569345490281 1106 6 -0.23321465559025256 1106 7 -0.18442950349326281 1106 8 -0.022211136031963008 1106 9 -0.09532340504465964 1106 10-11 -0.08113852153444867 1106 12-13 -0.19805562038562385 1106 14-15 -0.23401780429968966 1106 16-17 -0.06476821647641628 1106 18-19 0.04861921240179612 1106 20-21 -0.018987349428599032 1106 22-23 -0.12729022478814045 1106 24-25 0.004659899332857265 1106 26-27 -0.20605015267165783 1106 28-29 -0.22308004240014867 1106 30-31 -0.21015113822691234 1106 32-33 -0.1353752175973213 1106 34-35 -0.4357261144408824 1106 36-37 -0.33669116299078183 1106 38-39 -0.3350260084555998 1106 40-41 -0.3463120562968598 1106 42-43 -0.1833368872222252 1106 44-45 -0.16040354167601123 1106 46-47 -0.1594560655229813 1106 48-49 -0.431853070566369 1106 50-51 -0.5909564626953667 1106 52-53 -0.37374744326857723 1106 54-55 -0.2585217900388628 1106 56-57 -0.4421282268090039 1106 58-59 -0.5367718744679948 1106 60-61 -0.3864631141625452 1106 62-63 -0.14207425094100756 1106 64-65 -0.34199515767524247 1106 66-67 -0.5324905693829152 1106 68-69 -0.5939072134081016 1106 70-71 -0.6198586598328824 1106 72-73 -0.24703202520538525 1106 74-75 -0.09754070283891636 1106 76-77 -0.3972250992078834 1106 78-79 -0.09861905794748438 1106 80-81 -0.036216922550938335 1106 82-83 -0.05298886891072385 1106 84-85 0.12045093135356666 1106 86-87 0.003907271906630427 1106 88-89 -0.04068471470021251 1106 90-91 0.040993229471336434 1106 92-93 0.28988265745706343 1106 94-95 0.3524358550630815 1106 96-97 0.3815268608965603 1106 98-99 0.7320161777630965 1106 100-101 1.5225009381031285 1107 1 -0.01775507225087125 1107 2 0.10267137024772666 1107 3 0.011928970733848132 1107 4 -0.050930995171583504 1107 5 -0.27931378130743667 1107 6 -0.15272940070525465 1107 7 -0.0981884678687095 1107 8 -0.29219344300323513 1107 9 -0.020611956712187407 1107 10-11 -0.09073102723338167 1107 12-13 -0.08160540213462752 1107 14-15 -0.10788911108793542 1107 16-17 0.06471050474468143 1107 18-19 -0.007481970646175284 1107 20-21 -0.0032286290949343766 1107 22-23 -0.09899524483839173 1107 24-25 -0.24508704598515152 1107 26-27 -0.5834503589978866 1107 28-29 -0.4957738476215354 1107 30-31 -0.38630839541921347 1107 32-33 -0.520683902711319 1107 34-35 -0.38196620717675955 1107 36-37 -0.42647565746379 1107 38-39 -0.5507915231341585 1107 40-41 -0.5062419220184111 1107 42-43 -0.33762536678649724 1107 44-45 -0.31124007805764364 1107 46-47 -0.4038729166075825 1107 48-49 -0.3855288111499604 1107 50-51 -0.48264212579569943 1107 52-53 -0.33951642444963426 1107 54-55 -0.5557813118279142 1107 56-57 -0.46456481754539425 1107 58-59 -0.3076887505114101 1107 60-61 -0.18944791146921602 1107 62-63 -0.24095138145344208 1107 64-65 -0.1506636324696089 1107 66-67 -0.09955837971808279 1107 68-69 -0.42799139118144325 1107 70-71 -0.46300774162570235 1107 72-73 -0.4956870222380587 1107 74-75 -0.6609007421074544 1107 76-77 -0.6633831606287615 1107 78-79 -0.48641877175303705 1107 80-81 -0.6123080388756676 1107 82-83 -0.6430492969821842 1107 84-85 -0.600638437853906 1107 86-87 -0.3201240486366963 1107 88-89 -0.22849358007234954 1107 90-91 0.019385132830791463 1107 92-93 0.061732715971622554 1107 94-95 0.10541150080316797 1107 96-97 0.08077310459445997 1107 98-99 0.5247227951776914 1107 100-101 1.5965690035728812 1108 1 0.03958032434474035 1108 2 0.09319557673559586 1108 3 0.009624202173398544 1108 4 -0.03797950798259109 1108 5 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#Quality Count 2 1.0 3 3.0 4 5.0 5 11.0 6 41.0 7 80.0 8 258.0 9 498.0 10 879.0 11 1607.0 12 2733.0 13 4148.0 14 5575.0 15 8430.0 16 12639.0 17 19957.0 18 18213.0 19 8757.0 20 6255.0 21 5703.0 22 5684.0 23 5752.0 24 6350.0 25 7162.0 26 8387.0 27 10186.0 28 12410.0 29 15175.0 30 19079.0 31 23533.0 32 30408.0 33 40393.0 34 56895.0 35 87183.0 36 132111.0 37 212516.0 38 184248.0 39 17806.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.487724572180916 19.887315992207515 16.00601228624499 19.618947149366576 2 15.45570256376458 21.351454886970274 41.32937619455612 21.863466354709022 3 19.26170253081131 26.88492717326831 31.80083454161998 22.0525357543004 4 11.755886026871362 17.97458682217881 38.68429805853537 31.58522909241446 5 11.603870943820642 39.38868667643054 34.148328502893115 14.859113876855703 6 28.59468710867989 38.54276510907533 17.24846355368088 15.614084228563897 7 25.78686235418177 36.49657285968497 20.360076286825283 17.356488499307982 8 26.505655605351613 34.30754877084294 19.24697653727015 19.939819086535294 9 26.98234528438674 16.681461312858367 21.15445610624135 35.181737296513546 10-11 24.33733029064786 28.272414403875306 26.178233951756408 21.212021353720427 12-13 26.535487116588424 26.21505598919916 27.776945333100596 19.472511561111823 14-15 22.99731636789033 26.338057322217097 28.871958001054505 21.792668308838067 16-17 19.45128682528175 29.985212219073354 29.29973266657879 21.263768289066103 18-19 19.16299718249522 26.486501515850524 35.08627578593554 19.26422551571871 20-21 20.38263917744471 25.182079795360075 35.49007462375325 18.945206403441972 22-23 19.854686142767182 25.471631565370384 35.545565435259334 19.1281168566031 24-25 19.685758199095325 25.449518184884163 35.87026648507957 18.994457130940948 26-27 19.515115003784544 25.672593210477263 36.00362492340805 18.808666862330146 28-29 19.10866725400249 26.091458588962585 35.82739947366334 18.972474683371583 30-31 19.365349236074245 25.84073539535533 35.69399512599253 19.0999202425779 32-33 19.116038769524817 25.892158356949846 35.681854692545976 19.309948180979372 34-35 19.645762621103273 25.665501631186977 35.646532903842356 19.042202843867393 36-37 19.611573601133593 25.42828956040335 35.76722426349436 19.192912574968695 38-39 19.483673713174717 26.063000477822445 35.59468298952086 18.858642819481975 40-41 19.311492865616557 26.19270249785804 35.44999752850458 19.045807108020828 42-43 19.302072423065557 26.19236675183756 34.909664270141064 19.595896554955825 44-45 19.535987032887366 26.45483548078824 34.3473501449944 19.661827341329992 46-47 19.894765784617412 27.04449309628946 33.52629877084294 19.53444234825018 48-49 20.012522243458776 27.345603621564624 32.752514746589334 19.889359388387266 50-51 20.088881154023593 27.764470605681147 32.402695165754956 19.7439530745403 52-53 20.194239044189846 28.242925469442774 31.629442322218292 19.933393164149088 54-55 19.817428573782376 27.91584970012522 31.88496836485863 20.38175336123377 56-57 20.331036215646215 28.064499851710274 31.39679653002043 20.20766740262308 58-59 20.415118549396954 27.722661141501355 31.785130247808606 20.077090061293088 60-61 20.2873731299018 27.57256928425493 32.405166661174455 19.734890924668818 62-63 20.07559686614381 27.75782846174125 32.35352270480459 19.813051967310354 64-65 20.02920483754037 27.92589015026692 32.09365525934225 19.951249752850458 66-67 19.93889227575298 27.94056465432017 31.774523413299942 20.346019656626904 68-69 20.183673301258814 27.890465382587493 31.728955216502996 20.196906099650697 70-71 20.403893841033415 28.090862469518225 31.561511401832202 19.94373228761616 72-73 20.266334799412196 27.80986148283699 31.62837732771342 20.29542639003739 74-75 20.101001883487406 28.24577193634657 31.17202552645481 20.481200653711213 76-77 19.942342071442695 27.96692727212812 31.52845515059645 20.56227550583273 78-79 19.984563451525737 27.96399237131747 31.471713734923878 20.579730442232915 80-81 19.95469954854017 28.28410251763 31.26632216766625 20.49487576616358 82-83 19.906361169079723 28.317945691949564 31.287294498911773 20.488398640058943 84-85 19.694975645422062 28.156312109322705 31.232712368830263 20.915999876424973 86-87 19.49958396493772 28.200329120806693 31.159327011797274 21.140759902458313 88-89 19.56157730837672 27.96733918803137 31.003571310881174 21.467512192710736 90-91 19.41788267231853 27.629563947530762 31.413934194089226 21.53861918606148 92-93 19.564315181773058 27.473363774733638 31.755601691371982 21.20671935212132 94-95 19.26314423647268 27.582249307981282 31.652802263889807 21.50180419165623 96-97 19.088337425031305 27.455379209780535 31.799701772886046 21.656581592302114 98-99 19.107263581789375 27.85235469515344 31.23496182820918 21.805419894848004 100-101 18.787543288232037 27.752101651711573 31.580982787697774 21.879372272358612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 820.0 1 602.0 2 608.5 3 892.5 4 695.0 5 558.0 6 1346.5 7 3402.5 8 5673.0 9 6316.0 10 4969.5 11 3594.0 12 3391.5 13 3564.0 14 3780.0 15 3885.5 16 3913.0 17 4016.5 18 4206.5 19 4288.0 20 4479.0 21 4962.0 22 5502.0 23 6184.5 24 7011.0 25 8293.0 26 9784.5 27 11197.5 28 12311.5 29 13729.5 30 15913.5 31 17901.0 32 20113.5 33 21989.5 34 24234.0 35 26223.5 36 28244.5 37 30631.0 38 31090.0 39 31029.5 40 31118.5 41 31263.5 42 31484.5 43 32150.0 44 33128.5 45 33584.0 46 33491.0 47 34880.0 48 39942.5 49 37901.5 50 30526.5 51 26815.5 52 24109.5 53 22832.0 54 21515.5 55 19513.5 56 17397.0 57 15269.5 58 13648.0 59 12018.5 60 9727.0 61 7576.0 62 5881.0 63 4212.0 64 2749.5 65 1910.5 66 1391.0 67 1074.0 68 754.5 69 490.5 70 372.5 71 264.5 72 202.0 73 221.5 74 201.5 75 169.0 76 144.5 77 88.0 78 49.5 79 28.0 80 16.5 81 11.0 82 7.5 83 2.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040470737494233175 2 0.0 3 0.0 4 1.0297897581229816E-4 5 0.015034930468595532 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003758732617148883 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.148948790614908E-5 22-23 1.5446846371844724E-4 24-25 4.634053911553417E-4 26-27 0.0017506425888090687 28-29 0.002111069004152112 30-31 2.574474395307454E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008547254992420747 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.148948790614908E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0297897581229816E-4 74-75 1.0297897581229816E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.148948790614908E-5 84-85 2.059579516245963E-4 86-87 0.0 88-89 0.0 90-91 0.00669363342779938 92-93 0.0026774533711197523 94-95 0.0 96-97 0.0 98-99 1.5446846371844724E-4 100-101 0.01127619785144665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 971072.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.063817472186415 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28761453157131 39.71824438360244 2 13.1121013369913 13.653321013718529 3 4.460375427542261 6.9667251635095715 4 2.017838116036529 4.202254214469854 5 1.0738570608405573 2.795454900341068 6 0.6654496242685984 2.07875086649132 7 0.45760466452603427 1.6677252009813182 8 0.30652928900073934 1.2767267961930857 9 0.221023719465134 1.0356604728530827 >10 1.2515079226459227 11.99718017536158 >50 0.09199081086353905 3.296904039920892 >100 0.0484963485652969 4.7380573101829295 >500 0.0034067682360059857 1.2544187121085384 >1k 0.0016031850522381108 1.477486765546786 >5k 2.0039813152976385E-4 0.9445440073790732 >10k+ 4.007962630595277E-4 2.8965459773398683 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15314 1.5770200355895339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12448 1.2818822909114875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9053 0.9322686680287353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2841 0.2925632702827391 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 0.25425509128056417 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2300 0.23685164436828576 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1623 0.16713487774335992 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1542 0.15879358070256377 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1197 0.1232658340473209 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1130 0.11636624266789693 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 0.10905473538522376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.574474395307454E-4 0.0 18-19 0.0 0.0 0.0 3.6042641534304355E-4 0.0 20-21 0.0 0.0 0.0 4.119159032491926E-4 0.0 22-23 0.0 0.0 0.0 5.663843669676399E-4 0.0 24-25 0.0 0.0 0.0 7.723423185922362E-4 0.0 26-27 0.0 0.0 0.0 0.001390216173466025 0.0 28-29 0.0 0.0 0.0 0.0026774533711197523 0.0 30-31 0.0 0.0 0.0 0.0050974593027087595 0.0 32-33 0.0 0.0 0.0 0.00844427601660845 0.0 34-35 0.0 0.0 0.0 0.011739603242601992 0.0 36-37 0.0 0.0 0.0 0.016528125617873854 0.0 38-39 0.0 0.0 0.0 0.02167707440848876 0.0 40-41 0.0 0.0 0.0 0.02698049166282212 0.0 42-43 0.0 0.0 0.0 0.039132010808673304 0.0 44-45 0.0 0.0 0.0 0.05334310947077045 0.0 46-47 0.0 0.0 0.0 0.0670393132538061 0.0 48-49 0.0 0.0 0.0 0.08784106636789032 0.0 50-51 0.0 0.0 0.0 0.10972409872800369 0.0 52-53 0.0 0.0 0.0 0.16327316615039872 0.0 54-55 0.0 0.0 0.0 0.20812051011665458 0.0 56-57 0.0 0.0 0.0 0.29369603901667435 0.0 58-59 0.0 0.0 0.0 0.39219542938113755 0.0 60-61 0.0 0.0 0.0 0.5024344229882027 0.0 62-63 0.0 0.0 0.0 0.6523203222830027 0.0 64-65 0.0 0.0 0.0 0.7292456172147894 0.0 66-67 0.0 0.0 0.0 0.9003451855269229 0.0 68-69 0.0 0.0 0.0 1.1346223554999013 0.0 70-71 0.0 0.0 0.0 1.3103559777235878 0.0 72-73 0.0 0.0 0.0 1.5956077407236537 0.0 74-75 0.0 0.0 0.0 1.8852875996836487 0.0 76-77 0.0 0.0 0.0 2.0742025308113092 0.0 78-79 0.0 0.0 0.0 2.284640067883741 0.0 80-81 0.0 0.0 0.0 2.653665227707111 0.0 82-83 0.0 0.0 0.0 2.962705134119818 0.0 84-85 5.148948790614908E-5 0.0 0.0 3.4036096190601723 0.0 86-87 1.0297897581229816E-4 0.0 0.0 3.9796225202662625 0.0 88-89 1.0297897581229816E-4 0.0 0.0 4.726940947736111 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8500 0.0 31.303246 1 GTATCAA 12570 0.0 30.957716 1 CACGTCA 70 0.008387434 27.145184 9 GTGTAAG 485 0.0 25.471344 1 GTACATG 6175 0.0 25.39202 1 TCAACGC 15680 0.0 24.84141 4 TATCAAC 15685 0.0 24.772923 2 ATCAACG 15730 0.0 24.702051 3 TACATGG 6200 0.0 24.670197 2 CAACGCA 15915 0.0 24.474602 5 AACGCAG 16175 0.0 24.078712 6 GAGTACT 10210 0.0 23.821823 12-13 ATCGCGT 50 0.001654718 23.748365 24-25 TCGCGAT 50 0.001654718 23.748365 50-51 AGTACTT 10505 0.0 23.243305 12-13 ACATGGG 6320 0.0 23.149525 3 CGGGTCG 105 0.0021968568 22.61749 6 GTACTTT 10930 0.0 22.140514 14-15 CGCAGAG 17610 0.0 22.012106 8 ACGCAGA 17675 0.0 22.011786 7 >>END_MODULE