FastQCFastQC Report
Thu 26 May 2016
SRR937591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642903
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115070.3158744550705852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88920.24409104497155154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57650.15825290983591933No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49280.13527672847726113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67850.047.5221331
GTATCAA117400.041.5213851
ATCAACG148800.032.074623
TCAACGC151300.031.4843984
TATCAAC152650.031.4840852
CAACGCA153950.030.8823835
AACGCAG160000.029.7189946
CGCAGAG183100.025.7109018
ACGCAGA185250.025.5410677
GTACATG133100.023.1533071
TACATGG129600.023.0718482
GCAGAGT201900.022.7056989
ACATGGG127850.022.309063
GAGTACT113150.021.45321312-13
AGTACTT118800.020.19300512-13
GTACTTT119950.020.09620314-15
AGAGTAC183600.020.05256710-11
CAGAGTA191650.019.65645810-11
GGACCGA9650.018.70586
TGGTATC37250.018.376932
Produced by FastQC (version 0.11.5)