##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3642903 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6445008280484 33.0 31.0 34.0 30.0 34.0 2 31.794180355611996 33.0 31.0 34.0 30.0 34.0 3 31.817461513523693 33.0 31.0 34.0 30.0 34.0 4 35.4129223314483 37.0 35.0 37.0 33.0 37.0 5 35.40264014715736 37.0 35.0 37.0 33.0 37.0 6 35.45564238191354 37.0 35.0 37.0 33.0 37.0 7 35.39461165998655 37.0 35.0 37.0 33.0 37.0 8 35.37602812921453 37.0 35.0 37.0 33.0 37.0 9 36.996922234822065 39.0 37.0 39.0 33.0 39.0 10-11 36.871983552677634 39.0 37.0 39.0 33.0 39.0 12-13 36.7532278515239 39.0 37.0 39.0 33.0 39.0 14-15 37.898607923406146 40.0 38.0 41.0 33.0 41.0 16-17 37.80034810150037 40.0 38.0 41.0 33.0 41.0 18-19 37.72495081532503 40.0 37.5 41.0 32.0 41.0 20-21 37.60782815243777 40.0 37.0 41.0 32.0 41.0 22-23 37.52249442271727 40.0 37.0 41.0 32.0 41.0 24-25 37.5670233327651 40.0 37.0 41.0 32.0 41.0 26-27 37.48230175220147 40.0 37.0 41.0 32.0 41.0 28-29 37.358576937129534 40.0 37.0 41.0 32.0 41.0 30-31 37.131557853722704 39.5 36.5 41.0 31.0 41.0 32-33 37.00417606507777 39.0 36.0 41.0 31.0 41.0 34-35 37.00995044336893 40.0 36.0 41.0 31.0 41.0 36-37 36.876231950178195 39.5 36.0 41.0 30.5 41.0 38-39 36.72739103950887 39.0 36.0 41.0 30.0 41.0 40-41 36.50761686490142 39.0 36.0 41.0 30.0 41.0 42-43 36.25132991463127 39.0 35.0 41.0 30.0 41.0 44-45 36.03810985359753 39.0 35.0 41.0 29.0 41.0 46-47 35.72779195603067 39.0 35.0 40.0 27.5 41.0 48-49 35.43019893749573 39.0 35.0 40.0 26.5 41.0 50-51 34.5532696039395 38.0 34.0 39.5 25.0 40.5 52-53 34.650732259409594 38.0 34.0 39.5 26.0 40.5 54-55 34.9393787042916 38.0 34.0 40.0 26.0 41.0 56-57 34.70743223193151 38.0 34.0 40.0 25.0 41.0 58-59 34.45032011557815 38.0 34.0 40.0 24.0 41.0 60-61 34.123571777782715 38.0 34.0 40.0 23.0 41.0 62-63 33.737751458109095 37.5 33.0 40.0 22.5 41.0 64-65 33.333769935680415 37.0 33.0 39.5 21.5 41.0 66-67 32.88661556456485 36.0 32.0 39.0 19.5 41.0 68-69 32.36224132237394 36.0 31.0 39.0 18.0 40.0 70-71 31.89724499938648 35.0 31.0 38.5 15.5 40.0 72-73 31.42249340704378 35.0 31.0 37.5 11.5 40.0 74-75 30.86789148654246 35.0 30.0 37.0 7.5 39.0 76-77 30.36308295883805 34.0 30.0 36.5 6.5 39.0 78-79 29.79039271701717 34.0 29.0 36.0 2.0 38.5 80-81 29.228281675356165 34.0 29.0 35.5 2.0 37.0 82-83 28.702132749623033 34.0 28.0 35.0 2.0 37.0 84-85 28.171628368913474 33.5 27.0 35.0 2.0 36.0 86-87 27.612607033456563 33.0 26.0 35.0 2.0 36.0 88-89 27.114848652297358 33.0 25.5 35.0 2.0 35.5 90-91 26.536108976824252 32.0 25.0 35.0 2.0 35.0 92-93 25.876811433079606 32.0 23.5 34.0 2.0 35.0 94-95 25.113118164277225 31.5 19.5 34.0 2.0 35.0 96-97 24.254583775631687 31.0 12.0 34.0 2.0 35.0 98-99 23.16696093747212 31.0 2.0 34.0 2.0 35.0 100-101 20.341656365815943 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1621922697185063 1101 2 0.2912466829877225 1101 3 0.2672090561875251 1101 4 0.09128788398075471 1101 5 0.15033610971951816 1101 6 0.26679469210775153 1101 7 0.2594219019325834 1101 8 0.21625152113766433 1101 9 0.30474084769915066 1101 10-11 0.3474092195938354 1101 12-13 0.2799375566527402 1101 14-15 0.4307960836999527 1101 16-17 0.37112030815777786 1101 18-19 0.4448968542412288 1101 20-21 0.5489674014223667 1101 22-23 0.3768837213937317 1101 24-25 0.2987789298245147 1101 26-27 0.30074202373243963 1101 28-29 0.3609626436808355 1101 30-31 0.45440792989649736 1101 32-33 0.3856139062686026 1101 34-35 0.40317726285415745 1101 36-37 0.3040147898116672 1101 38-39 0.18633773482337546 1101 40-41 0.18646139163423925 1101 42-43 0.3431092869578407 1101 44-45 0.3273276596159107 1101 46-47 0.309666044634028 1101 48-49 0.5138870206997268 1101 50-51 0.3705167370224629 1101 52-53 0.35036343473632314 1101 54-55 0.4999811368550411 1101 56-57 0.2936784211925598 1101 58-59 0.21625715284582014 1101 60-61 0.3439366925399483 1101 62-63 0.39877315547799697 1101 64-65 0.35836583421176016 1101 66-67 0.4234167656776435 1101 68-69 0.4215209967724505 1101 70-71 0.46950928605215836 1101 72-73 0.42181316658444246 1101 74-75 0.37442699703253624 1101 76-77 0.43747835167787485 1101 78-79 0.5963383056793816 1101 80-81 0.6232033047376113 1101 82-83 0.5767892280696394 1101 84-85 0.5499348585485748 1101 86-87 0.669520085176007 1101 88-89 0.6369577733049887 1101 90-91 0.8696619075308547 1101 92-93 0.7749220401321537 1101 94-95 0.829340437007076 1101 96-97 1.137966929259342 1101 98-99 1.4243674657936474 1101 100-101 1.7489544136533013 1102 1 0.14423513311698954 1102 2 0.27585857045921713 1102 3 0.3209038208916013 1102 4 0.17460820135931954 1102 5 0.15308193384483815 1102 6 0.085588429202204 1102 7 0.15528806568755016 1102 8 0.1320652553871966 1102 9 0.22486774396106313 1102 10-11 0.22903631620848586 1102 12-13 0.3270048761005313 1102 14-15 0.37779376004211684 1102 16-17 0.4636413173033418 1102 18-19 0.3599097586306712 1102 20-21 0.46649769218330306 1102 22-23 0.47546133320776107 1102 24-25 0.521610371040893 1102 26-27 0.41688005669317363 1102 28-29 0.4431859407523433 1102 30-31 0.4916180653584874 1102 32-33 0.47439472657745796 1102 34-35 0.5808149848265458 1102 36-37 0.7981298350467583 1102 38-39 0.7134753032384751 1102 40-41 0.7251251303308877 1102 42-43 0.6883760427528074 1102 44-45 0.6698989506343764 1102 46-47 0.708969272683774 1102 48-49 0.7781022252563048 1102 50-51 0.8012594614911208 1102 52-53 0.8861028835230371 1102 54-55 0.8351083089312326 1102 56-57 0.8073931700357662 1102 58-59 0.8762354394441232 1102 60-61 0.9416815544143304 1102 62-63 0.9099205072425391 1102 64-65 0.8056859266002547 1102 66-67 0.7950940558426325 1102 68-69 0.8615549823600688 1102 70-71 0.6970977100958464 1102 72-73 0.7986086323216455 1102 74-75 0.7794297412296629 1102 76-77 0.891315253707095 1102 78-79 1.0319950954057013 1102 80-81 0.9701710056458488 1102 82-83 0.9989158940731144 1102 84-85 1.0214130665863017 1102 86-87 0.994160077812694 1102 88-89 1.1283844428103755 1102 90-91 1.2570599918302356 1102 92-93 1.3064887520137667 1102 94-95 1.493255817495971 1102 96-97 1.4806826353950306 1102 98-99 1.6968460971598063 1102 100-101 1.7956851776991414 1103 1 0.3367337394412324 1103 2 0.4193608541810683 1103 3 0.3263337573894347 1103 4 0.22428561177795103 1103 5 0.2873744149659885 1103 6 0.23844222885455224 1103 7 0.318279916314701 1103 8 0.3296119243494715 1103 9 0.2754203812662368 1103 10-11 0.3364448924936667 1103 12-13 0.3818526634736372 1103 14-15 0.4499901483554041 1103 16-17 0.4836990779586898 1103 18-19 0.34735502250863703 1103 20-21 0.45418535064271026 1103 22-23 0.42831361370513577 1103 24-25 0.3934578824341912 1103 26-27 0.42542440983773133 1103 28-29 0.38561127018928687 1103 30-31 0.4618498139401268 1103 32-33 0.4519287329361674 1103 34-35 0.4891670457166555 1103 36-37 0.42614530183170274 1103 38-39 0.4311380415987358 1103 40-41 0.4777288055838156 1103 42-43 0.5819536140082491 1103 44-45 0.3979316345090993 1103 46-47 0.39038631364094556 1103 48-49 0.528551530235454 1103 50-51 0.5023796074372342 1103 52-53 0.651978686050164 1103 54-55 0.6054058577746773 1103 56-57 0.562673909131469 1103 58-59 0.5128167062674649 1103 60-61 0.747322355580927 1103 62-63 0.7359403256618151 1103 64-65 0.781751453712026 1103 66-67 0.7856598055404049 1103 68-69 0.7031252509481547 1103 70-71 0.7738448984304114 1103 72-73 0.710386499395284 1103 74-75 0.627996090844718 1103 76-77 0.6415331109568676 1103 78-79 0.7813813270062369 1103 80-81 0.9025512951163606 1103 82-83 0.9820918484114856 1103 84-85 0.9858280898971366 1103 86-87 1.0020462049340928 1103 88-89 1.1793427838573933 1103 90-91 1.1401281382928588 1103 92-93 0.9767812593477849 1103 94-95 0.9197470356631605 1103 96-97 1.2837790753744862 1103 98-99 1.3972350753031897 1103 100-101 1.5086722325625317 1104 1 0.1731475112763512 1104 2 0.2305961675151984 1104 3 0.21242959971935704 1104 4 0.14481133928806145 1104 5 0.27047589263969485 1104 6 0.20734665194294166 1104 7 0.23107423883111267 1104 8 0.21662929608960724 1104 9 0.21851789179115144 1104 10-11 0.23035630047017008 1104 12-13 0.3740478409492738 1104 14-15 0.3366844457871849 1104 16-17 0.35293803220756104 1104 18-19 0.33353063159162133 1104 20-21 0.3830210132881504 1104 22-23 0.384894151509549 1104 24-25 0.2378023088652128 1104 26-27 0.31858647574681953 1104 28-29 0.42066860574925613 1104 30-31 0.4030289894422978 1104 32-33 0.5001176953111468 1104 34-35 0.41557762509732754 1104 36-37 0.4615640003362742 1104 38-39 0.5843930291954464 1104 40-41 0.5621129451370379 1104 42-43 0.47621017731245274 1104 44-45 0.5081583731217236 1104 46-47 0.5604526890364099 1104 48-49 0.4923475057800957 1104 50-51 0.5217739912452046 1104 52-53 0.45021363196207176 1104 54-55 0.6299872737642787 1104 56-57 0.6504319838331227 1104 58-59 0.6133500260795799 1104 60-61 0.6430747406655755 1104 62-63 0.6028875377509735 1104 64-65 0.6758521116371909 1104 66-67 0.8387032465631847 1104 68-69 0.6784252517064928 1104 70-71 0.6171214064710995 1104 72-73 0.72143031750538 1104 74-75 0.7208361084322092 1104 76-77 0.5523101799446621 1104 78-79 0.6349421255689052 1104 80-81 0.8218920450948382 1104 82-83 0.7248788269444226 1104 84-85 0.7344738617239273 1104 86-87 0.813136987959755 1104 88-89 0.9832005832823363 1104 90-91 1.050504068762482 1104 92-93 0.8624064116659298 1104 94-95 1.0116104699858077 1104 96-97 1.1771040352680693 1104 98-99 1.2005565364332327 1104 100-101 1.1810507583325673 1105 1 0.27656291076317174 1105 2 0.33818335756852136 1105 3 0.26457513424065127 1105 4 0.272612388110943 1105 5 0.28949127616360215 1105 6 0.1663105698344225 1105 7 0.25420073904985685 1105 8 0.22782964845625742 1105 9 0.31453551307977534 1105 10-11 0.3442619610985176 1105 12-13 0.3261123414872884 1105 14-15 0.3965469060276874 1105 16-17 0.4048549126985108 1105 18-19 0.4311749555915725 1105 20-21 0.373110294779444 1105 22-23 0.46961261292067036 1105 24-25 0.4798051082881898 1105 26-27 0.3571957507938208 1105 28-29 0.36650269042367256 1105 30-31 0.49090799790855755 1105 32-33 0.41518957278687907 1105 34-35 0.48220991483022146 1105 36-37 0.4357442748984752 1105 38-39 0.53318908517209 1105 40-41 0.5260507533100593 1105 42-43 0.46855244741686164 1105 44-45 0.4157331475715935 1105 46-47 0.4379385071655548 1105 48-49 0.4452679439326559 1105 50-51 0.4378757356374834 1105 52-53 0.41126669684246764 1105 54-55 0.5318216052727109 1105 56-57 0.4717357182692794 1105 58-59 0.6443891086256031 1105 60-61 0.69692180407975 1105 62-63 0.6375182400602739 1105 64-65 0.6554553813750417 1105 66-67 0.7621528850326413 1105 68-69 0.7139864961046882 1105 70-71 0.7819185517691309 1105 72-73 0.8616719487974365 1105 74-75 0.7808093114214145 1105 76-77 0.8495265351373398 1105 78-79 0.7398902978147106 1105 80-81 0.6986132320265277 1105 82-83 0.9139426756632503 1105 84-85 0.7868714403856423 1105 86-87 0.894585933224679 1105 88-89 1.000663905520451 1105 90-91 0.8841634718084386 1105 92-93 0.9254191465277586 1105 94-95 1.259080569074147 1105 96-97 1.22840335034061 1105 98-99 1.2117142840654012 1105 100-101 1.268574813564264 1106 1 0.14532546222416087 1106 2 0.20479333346971984 1106 3 0.11295348548073392 1106 4 0.2591710285366915 1106 5 0.0882185580192072 1106 6 0.17007977356973214 1106 7 0.23872667036826556 1106 8 0.3161015633071429 1106 9 0.3292326463260835 1106 10-11 0.1508900738608432 1106 12-13 0.04742754632712831 1106 14-15 0.19425846090268095 1106 16-17 0.0697848755461905 1106 18-19 0.22599339870835422 1106 20-21 0.07671978117365796 1106 22-23 0.1912626279824181 1106 24-25 0.08740062490632283 1106 26-27 0.23765940176279088 1106 28-29 0.26217275016863084 1106 30-31 0.26488599519745293 1106 32-33 0.2628255051094044 1106 34-35 0.4325566489400643 1106 36-37 0.37775275968611055 1106 38-39 0.45869051603863653 1106 40-41 0.43085702150967364 1106 42-43 0.5278301735941895 1106 44-45 0.4907977875956888 1106 46-47 0.5248018343068424 1106 48-49 0.6334987527487996 1106 50-51 0.6733144719563953 1106 52-53 0.6295020869418764 1106 54-55 0.5790998209570191 1106 56-57 0.6881803286719261 1106 58-59 0.6197323910836801 1106 60-61 0.61185051178132 1106 62-63 0.7467068508645696 1106 64-65 0.8133398953824766 1106 66-67 0.8853482987287933 1106 68-69 0.7000932839347982 1106 70-71 0.5876184839912355 1106 72-73 0.8228063498417129 1106 74-75 0.7689601046737522 1106 76-77 0.8012451439334036 1106 78-79 0.8691053922016962 1106 80-81 0.7489383148661695 1106 82-83 0.6215005378984166 1106 84-85 0.9813158499649184 1106 86-87 1.0765664282542993 1106 88-89 1.2211287488782183 1106 90-91 1.2222624902841908 1106 92-93 1.2238477036118027 1106 94-95 1.3877688158860764 1106 96-97 1.4643485381104675 1106 98-99 1.5266834702286722 1106 100-101 1.4709511189162114 1107 1 0.24173206874289477 1107 2 0.30388827647717065 1107 3 0.23469125040430328 1107 4 0.29977056967008053 1107 5 0.2858000169324839 1107 6 0.25487912348768305 1107 7 0.2798105566833726 1107 8 0.33020480577109623 1107 9 0.33656152048840937 1107 10-11 0.3850902831130938 1107 12-13 0.41200884316135244 1107 14-15 0.37683483671417406 1107 16-17 0.48994769877406696 1107 18-19 0.5862188998605617 1107 20-21 0.544369243916293 1107 22-23 0.4857079991434503 1107 24-25 0.500404299749249 1107 26-27 0.43398498452212664 1107 28-29 0.4257622272650252 1107 30-31 0.4603818688035304 1107 32-33 0.5187352415827391 1107 34-35 0.5430942468654507 1107 36-37 0.5319034181559061 1107 38-39 0.493637313747584 1107 40-41 0.5581654655129356 1107 42-43 0.4850584105398852 1107 44-45 0.5393228051595074 1107 46-47 0.5770449736960828 1107 48-49 0.6203551349425496 1107 50-51 0.4920710702135054 1107 52-53 0.3866209520605679 1107 54-55 0.46334289917245286 1107 56-57 0.5200418381135066 1107 58-59 0.4831973447997058 1107 60-61 0.5673549244938769 1107 62-63 0.6160757074693919 1107 64-65 0.6768897514033014 1107 66-67 0.7091365213207155 1107 68-69 0.8032677156805974 1107 70-71 0.7620510969451999 1107 72-73 0.8828322793366965 1107 74-75 0.7672569344831786 1107 76-77 0.859033026600784 1107 78-79 0.9891075080585807 1107 80-81 1.0951935635156183 1107 82-83 1.0717453434855386 1107 84-85 0.9343331060233346 1107 86-87 1.0318260669126111 1107 88-89 1.1363067741121 1107 90-91 1.269965287756012 1107 92-93 1.2460944724366243 1107 94-95 1.2844259196784762 1107 96-97 1.373470120102919 1107 98-99 1.2983712397560403 1107 100-101 1.199635516316384 1108 1 -0.065282691599311 1108 2 -0.06365091862398486 1108 3 0.07587284054298138 1108 4 -0.011434019536295636 1108 5 0.03818431916746334 1108 6 0.10897791251332478 1108 7 0.1800503500463293 1108 8 0.004828035514769624 1108 9 0.10378854920492131 1108 10-11 0.14598631117797112 1108 12-13 0.14845491661952082 1108 14-15 0.19691063322710534 1108 16-17 0.25477515511413173 1108 18-19 0.3401098063944872 1108 20-21 0.2831561183283995 1108 22-23 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-1.784596268394548 2316 98-99 -1.8946410561747804 2316 100-101 -1.8808212474493615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14558.0 3 2959.0 4 2803.0 5 3216.0 6 3681.0 7 4413.0 8 5346.0 9 6671.0 10 8545.0 11 10933.0 12 14094.0 13 17576.0 14 20207.0 15 23657.0 16 28429.0 17 35384.0 18 32251.0 19 25186.0 20 25488.0 21 27606.0 22 30386.0 23 33691.0 24 38001.0 25 43489.0 26 50353.0 27 58668.0 28 69391.0 29 82838.0 30 100861.0 31 124226.0 32 155502.0 33 199830.0 34 266671.0 35 367482.0 36 521507.0 37 678384.0 38 464574.0 39 44045.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13483259934216 18.896325491120507 14.857978766762647 23.110863142774683 2 14.935771371187078 19.615745869129075 41.62208945100103 23.82639330868281 3 19.214895200164506 23.97522825390682 29.61372669872191 27.19614984720676 4 12.39109022368038 16.35660357309405 37.506999130648786 33.745307072576786 5 13.618301998838739 37.518855345943116 34.44470782507525 14.418134830142895 6 32.26429485185371 36.16805800923454 17.450189055908673 14.117458083003068 7 28.729370209486422 32.75302341969265 20.35978756228854 18.157818808532387 8 25.996522211745702 35.89490610191757 19.501551857112524 18.607019829224203 9 25.919686447087948 17.370281234958398 20.35615760159527 36.35387471635838 10-11 24.709275578268354 27.065658683553 27.287048392569325 20.93801734560932 12-13 25.45410463391497 24.09361289912676 28.007036177371518 22.44524628958675 14-15 22.528110199435574 25.598740612456545 26.252391022394228 25.620758165713646 16-17 21.911982551150235 29.008616462944094 26.819162102581444 22.260238883324227 18-19 21.802118698086144 27.67688444581417 29.094699459165536 21.426297396934157 20-21 22.526001684514497 26.72696082644344 29.350183062034823 21.396854427007238 22-23 22.1995220917404 26.574017681533014 29.223786173663285 22.002674053063302 24-25 21.95213283111861 26.725661769660565 29.410227743100393 21.91197765612043 26-27 21.776741375713733 27.098923883348665 29.205737436997804 21.9185973039398 28-29 21.780525312714627 27.23142931564339 29.260674050372653 21.727371321269327 30-31 21.89777407907261 26.961979025481043 29.17539825606143 21.96484863938491 32-33 21.733450220139265 26.915470153979204 29.219863091698194 22.13121653418334 34-35 22.13145857204178 27.031829213966525 29.080502974941613 21.75620923905008 36-37 22.04868993681721 26.841080501337224 29.083498910285936 22.02673065155963 38-39 21.858909534884198 27.119942088246678 29.122489820414444 21.898658556454677 40-41 22.225555849959804 26.976562045603202 28.929965538203124 21.867916566233873 42-43 21.967718442863713 26.946502867980488 28.896031031834912 22.189747657320886 44-45 22.02548833901133 27.187199474561375 28.785394874525537 22.00191731190176 46-47 22.073567924557626 27.3842780064573 28.335339121169252 22.206814947815822 48-49 22.18628036118499 27.35914417970136 28.312343054230006 22.142232404883643 50-51 22.28279450437647 27.572473803481206 28.094300041285113 22.05043165085721 52-53 22.28267680110023 27.57321207080806 27.99721640549032 22.14689472260138 54-55 22.226310851418983 27.537687695925893 28.076341674274403 22.159659778380714 56-57 22.1839216529017 27.812262955934088 27.997931576768487 22.005883814395723 58-59 22.24239507785378 27.543945278933073 28.041618258687656 22.172041384525485 60-61 22.20039300833957 27.565150694212537 28.147899049677623 22.08655724777027 62-63 22.13555408834934 27.531794636716594 28.273914689811004 22.05873658512306 64-65 22.146534859289563 27.452514600924115 28.184585323306028 22.216365216480295 66-67 22.045144451355423 27.620832584033 28.020244032682147 22.313778931929434 68-69 22.13052349400067 27.591183447093933 28.14108706649182 22.137205992413577 70-71 22.152480116923286 27.441952552850246 28.007553816127682 22.398013514098782 72-73 22.150461469379437 27.444617693768127 27.98851760738483 22.41640322946761 74-75 22.1026923372532 27.71070969100894 27.78395195571421 22.402646016023652 76-77 22.239895281623134 27.644922753697315 27.785308077724796 22.32987388695475 78-79 22.251920997743188 27.621158331079453 27.82295539193194 22.303965279245414 80-81 22.135442446240994 27.70911015783974 27.733061101028433 22.42238629489083 82-83 22.2632718949385 27.683011100047818 27.77666869875608 22.277048306257598 84-85 22.277889809522815 27.647609232784493 27.681759514923016 22.392741442769683 86-87 22.267891968641024 27.630677751684708 27.74437094942206 22.35705933025221 88-89 22.405830025505743 27.522046684638372 27.691535040975268 22.380588248880617 90-91 22.31368892235172 27.482257168148067 27.84713707181471 22.356916837685503 92-93 22.273723943837016 27.379515873429405 27.824746461905047 22.52201372082853 94-95 22.13100374241412 27.56792577866137 27.75189801715296 22.549172461771555 96-97 22.224583920282516 27.471861498049215 27.71154024356409 22.59201433810418 98-99 22.097701765761485 27.353587866528738 27.704848295861705 22.843862071848076 100-101 22.52484550001526 26.844755523139053 28.12507302648418 22.50532595036151 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4249.0 1 2394.5 2 485.5 3 411.0 4 375.5 5 372.5 6 527.0 7 1128.0 8 1973.0 9 2465.0 10 2456.5 11 2454.0 12 2713.0 13 3059.5 14 3349.0 15 3603.5 16 3886.0 17 4212.0 18 4454.0 19 4930.5 20 5694.5 21 6578.5 22 8049.5 23 10319.5 24 13079.5 25 16555.0 26 21762.5 27 27839.5 28 32569.0 29 37885.5 30 44707.0 31 51977.0 32 60174.5 33 67807.0 34 75353.5 35 82454.5 36 91414.0 37 100297.5 38 105576.0 39 109396.0 40 113841.5 41 120573.0 42 125273.0 43 128800.0 44 133248.5 45 135634.0 46 137865.5 47 141754.5 48 144503.5 49 144012.0 50 140541.5 51 133709.0 52 126390.5 53 122101.0 54 119040.0 55 109425.0 56 95842.5 57 84874.5 58 76489.5 59 67064.0 60 54470.0 61 43305.5 62 33589.5 63 25153.0 64 18751.5 65 13946.0 66 9901.0 67 7090.5 68 5332.5 69 3918.0 70 2846.0 71 1918.0 72 1361.5 73 1058.5 74 762.5 75 494.5 76 333.0 77 243.0 78 162.5 79 102.5 80 77.5 81 59.0 82 43.0 83 33.0 84 28.0 85 15.5 86 10.5 87 7.5 88 5.5 89 5.0 90 6.0 91 5.0 92 4.5 93 5.0 94 4.0 95 3.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3188391236329927 2 0.28150626025452774 3 0.2804356854958806 4 0.28298859453573155 5 0.2921845572061622 6 0.19780927463618989 7 0.19769947209684144 8 0.1992641582825565 9 0.1963543909898232 10-11 0.20474055993255927 12-13 0.19550342130987292 14-15 0.19675242519496128 16-17 0.20210529898819704 18-19 0.19579165297566253 20-21 0.18777606760322743 22-23 0.18802312331676138 24-25 0.19237404893844276 26-27 0.1871996042716482 28-29 0.19874259622065152 30-31 0.20303862057265867 32-33 0.20524839667704575 34-35 0.20460330675837374 36-37 0.1819428077003423 38-39 0.1739134970104886 40-41 0.18000753794432628 42-43 0.18927212720184974 44-45 0.19444657186864434 46-47 0.19694457963882103 48-49 0.19470735289959681 50-51 0.1979190771755383 52-53 0.22055212559873266 54-55 0.18673294347941738 56-57 0.1733919349485836 58-59 0.172335085507355 60-61 0.16702338766637487 62-63 0.17464093883367193 64-65 0.17108608162226663 66-67 0.16956257138880723 68-69 0.17948597588242124 70-71 0.17377624383630308 72-73 0.17031746384682767 74-75 0.17407820081951125 76-77 0.17796246564896184 78-79 0.1805291000062313 80-81 0.17302135137828267 82-83 0.17162136900159022 84-85 0.1657194825116123 86-87 0.16256265950534504 88-89 0.16676260663542236 90-91 0.17469584010334616 92-93 0.16534889894131136 94-95 0.17055079424294306 96-97 0.16909591059657642 98-99 0.17252723995121472 100-101 0.15163730684017662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3642903.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.887531497246066 #Duplication Level Percentage of deduplicated Percentage of total 1 70.47170519781395 35.15659412720583 2 15.291098014119742 15.256702676137508 3 5.938888899609458 8.888295211137356 4 2.794985135181739 5.577396358628542 5 1.5823298014279528 3.9469263903884055 6 0.9658382051768619 2.890997032920258 7 0.6223955719904175 2.1734845090992905 8 0.414921023565085 1.6559508505578202 9 0.2990033314321723 1.3424884303143596 >10 1.4567529296425255 12.893356090720825 >50 0.10479029116860737 3.5865363883826986 >100 0.05480904809321744 4.904495959651733 >500 0.0020412084216853846 0.7078620109547218 >1k 2.7583897289347155E-4 0.2988322570372601 >5k 1.1033558915738846E-4 0.40338776859413505 >10k+ 5.516779457869423E-5 0.3166939382692715 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11507 0.3158744550705852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8892 0.24409104497155154 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5765 0.15825290983591933 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4928 0.13527672847726113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 2.745063483710656E-5 0.0 0.0 0.0 16-17 0.0 2.745063483710656E-5 0.0 1.372531741855328E-5 0.0 18-19 0.0 2.745063483710656E-5 0.0 2.745063483710656E-5 0.0 20-21 2.745063483710656E-5 2.745063483710656E-5 0.0 5.490126967421312E-5 0.0 22-23 2.745063483710656E-5 2.745063483710656E-5 0.0 1.0980253934842623E-4 0.0 24-25 5.490126967421312E-5 2.745063483710656E-5 0.0 1.5097849160408607E-4 0.0 26-27 5.490126967421312E-5 2.745063483710656E-5 0.0 2.4705571353395906E-4 0.0 28-29 8.235190451131968E-5 2.745063483710656E-5 0.0 9.607722192987295E-4 0.0 30-31 8.235190451131968E-5 2.745063483710656E-5 0.0 0.0021274241998757585 0.0 32-33 8.235190451131968E-5 2.745063483710656E-5 0.0 0.003294076180452787 0.0 34-35 8.235190451131968E-5 2.745063483710656E-5 0.0 0.004748959826819435 0.0 36-37 8.235190451131968E-5 2.745063483710656E-5 0.0 0.006684229582835448 0.0 38-39 8.235190451131968E-5 2.745063483710656E-5 0.0 0.008921456322059632 0.0 40-41 8.235190451131968E-5 2.745063483710656E-5 0.0 0.011899850201885694 0.0 42-43 8.235190451131968E-5 2.745063483710656E-5 0.0 0.01618214923647432 0.0 44-45 8.235190451131968E-5 2.745063483710656E-5 0.0 0.02234481675740474 0.0 46-47 8.235190451131968E-5 2.745063483710656E-5 0.0 0.03517798854375206 0.0 48-49 8.235190451131968E-5 2.745063483710656E-5 0.0 0.0590188648997791 0.0 50-51 8.235190451131968E-5 2.745063483710656E-5 0.0 0.07745196619289615 1.372531741855328E-5 52-53 8.235190451131968E-5 2.745063483710656E-5 0.0 0.09625565105631416 2.745063483710656E-5 54-55 8.235190451131968E-5 2.745063483710656E-5 0.0 0.1238572643850248 2.745063483710656E-5 56-57 8.235190451131968E-5 2.745063483710656E-5 0.0 0.14640796090370783 2.745063483710656E-5 58-59 8.235190451131968E-5 2.745063483710656E-5 0.0 0.17222528296800654 2.745063483710656E-5 60-61 8.235190451131968E-5 2.745063483710656E-5 0.0 0.21333260863657363 2.745063483710656E-5 62-63 8.235190451131968E-5 2.745063483710656E-5 0.0 0.24554592861791819 2.745063483710656E-5 64-65 8.235190451131968E-5 2.745063483710656E-5 0.0 0.2802435310520209 2.745063483710656E-5 66-67 8.235190451131968E-5 2.745063483710656E-5 0.0 0.3403604213452842 2.745063483710656E-5 68-69 8.235190451131968E-5 2.745063483710656E-5 0.0 0.40898700843805064 2.745063483710656E-5 70-71 8.235190451131968E-5 2.745063483710656E-5 0.0 0.46907644809647686 2.745063483710656E-5 72-73 8.235190451131968E-5 2.745063483710656E-5 0.0 0.5401873176420015 2.745063483710656E-5 74-75 8.235190451131968E-5 2.745063483710656E-5 0.0 0.6097060503669738 2.745063483710656E-5 76-77 8.235190451131968E-5 2.745063483710656E-5 0.0 0.6743660207257782 2.745063483710656E-5 78-79 8.235190451131968E-5 2.745063483710656E-5 0.0 0.7554689213520096 2.745063483710656E-5 80-81 8.235190451131968E-5 2.745063483710656E-5 0.0 0.8760046589217445 2.745063483710656E-5 82-83 8.235190451131968E-5 2.745063483710656E-5 0.0 0.9834464436741797 2.745063483710656E-5 84-85 8.235190451131968E-5 2.745063483710656E-5 0.0 1.10620568266572 2.745063483710656E-5 86-87 8.235190451131968E-5 2.745063483710656E-5 0.0 1.3001581431072964 2.745063483710656E-5 88-89 8.235190451131968E-5 2.745063483710656E-5 0.0 1.5493275555237127 2.745063483710656E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6785 0.0 47.522133 1 GTATCAA 11740 0.0 41.521385 1 ATCAACG 14880 0.0 32.07462 3 TCAACGC 15130 0.0 31.484398 4 TATCAAC 15265 0.0 31.484085 2 CAACGCA 15395 0.0 30.882383 5 AACGCAG 16000 0.0 29.718994 6 CGCAGAG 18310 0.0 25.710901 8 ACGCAGA 18525 0.0 25.541067 7 GTACATG 13310 0.0 23.153307 1 TACATGG 12960 0.0 23.071848 2 GCAGAGT 20190 0.0 22.705698 9 ACATGGG 12785 0.0 22.30906 3 GAGTACT 11315 0.0 21.453213 12-13 AGTACTT 11880 0.0 20.193005 12-13 GTACTTT 11995 0.0 20.096203 14-15 AGAGTAC 18360 0.0 20.052567 10-11 CAGAGTA 19165 0.0 19.656458 10-11 GGACCGA 965 0.0 18.7058 6 TGGTATC 3725 0.0 18.37693 2 >>END_MODULE