FastQCFastQC Report
Thu 26 May 2016
SRR937591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642903
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146510.40217925099844826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122190.33541930707460504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81310.22320111186051342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79200.049.6221
GTATCAA135300.040.040641
ATCAACG172500.031.1557083
TCAACGC175450.030.631864
TATCAAC176800.030.5054722
CAACGCA177850.030.2184985
AACGCAG183650.029.3284766
CGCAGAG210050.025.39648
ACGCAGA211150.025.3540827
GCAGAGT231500.022.6533879
GAGTACT139950.022.28316512-13
GTACATG129250.021.2883471
TACATGG125100.021.1573872
AGTACTT147350.020.69664612-13
GTACTTT149450.020.62605314-15
TGGTATC40650.020.5738832
ACATGGG126450.020.5557173
AGAGTAC214800.020.3123410-11
CAGAGTA221800.020.12103310-11
GGACCGA9650.019.1957326
Produced by FastQC (version 0.11.5)