##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937591_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3642903 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.181741594546985 33.0 31.0 34.0 31.0 34.0 2 32.46470164042249 34.0 31.0 34.0 31.0 34.0 3 32.5638725488985 34.0 31.0 34.0 31.0 34.0 4 36.185822954934565 37.0 35.0 37.0 35.0 37.0 5 36.099505806221025 37.0 35.0 37.0 35.0 37.0 6 36.13584660365648 37.0 35.0 37.0 35.0 37.0 7 36.08189183187145 37.0 35.0 37.0 35.0 37.0 8 36.053813950028314 37.0 35.0 37.0 35.0 37.0 9 37.766256746336644 39.0 37.0 39.0 35.0 39.0 10-11 37.67996059735876 39.0 37.0 39.0 35.0 39.0 12-13 37.57961809578789 39.0 37.0 39.0 35.0 39.0 14-15 38.83216681860593 40.0 38.0 41.0 35.0 41.0 16-17 38.78711442495175 40.0 38.0 41.0 35.0 41.0 18-19 38.75182649661547 40.0 38.0 41.0 35.0 41.0 20-21 38.64537375823622 40.0 38.0 41.0 34.0 41.0 22-23 38.59373403574018 40.0 38.0 41.0 34.0 41.0 24-25 38.62962546628334 40.0 38.0 41.0 34.0 41.0 26-27 38.50486782107566 40.0 38.0 41.0 34.0 41.0 28-29 38.36389234080622 40.0 38.0 41.0 34.0 41.0 30-31 38.42483741675252 40.0 38.0 41.0 34.0 41.0 32-33 38.36373697021304 40.0 38.0 41.0 34.0 41.0 34-35 38.232267507534516 40.0 38.0 41.0 34.0 41.0 36-37 38.09699626918422 40.0 38.0 41.0 34.0 41.0 38-39 37.96934079770996 40.0 38.0 41.0 33.0 41.0 40-41 37.80296057841782 40.0 37.0 41.0 33.0 41.0 42-43 37.60059655719628 40.0 37.0 41.0 33.0 41.0 44-45 37.33507507611375 40.0 37.0 41.0 32.5 41.0 46-47 37.009324157135126 40.0 37.0 41.0 31.5 41.0 48-49 36.71507037107494 40.0 36.0 41.0 31.0 41.0 50-51 36.47245383695366 39.0 36.0 41.0 30.0 41.0 52-53 36.24690816637171 39.0 36.0 41.0 30.0 41.0 54-55 36.026594998549236 39.0 35.0 40.0 29.5 41.0 56-57 35.756957294772874 39.0 35.0 40.0 29.0 41.0 58-59 35.496520220274874 38.0 35.0 40.0 28.0 41.0 60-61 35.173408268076315 38.0 34.0 40.0 28.0 41.0 62-63 34.804148092880865 38.0 34.0 40.0 27.0 41.0 64-65 34.38992487584764 37.0 34.0 40.0 26.0 41.0 66-67 33.96157624839311 36.5 33.0 39.0 26.0 40.5 68-69 33.5074282790401 36.0 33.0 39.0 25.5 40.0 70-71 33.11052572632321 35.5 32.5 38.5 25.0 40.0 72-73 32.61482380947283 35.0 32.0 38.0 23.5 40.0 74-75 32.13330275332612 35.0 31.0 37.0 22.5 39.0 76-77 30.899133054050573 34.0 30.0 36.0 21.5 38.5 78-79 31.49136059895089 34.0 31.0 36.0 22.0 38.5 80-81 31.30263707268626 34.0 31.0 36.0 21.5 37.5 82-83 30.92071940976743 34.0 31.0 35.0 20.0 37.0 84-85 30.532665294683937 34.0 31.0 35.0 18.5 36.5 86-87 30.146854170972986 34.0 30.0 35.0 17.5 36.0 88-89 29.698408796501035 34.0 30.0 35.0 10.5 36.0 90-91 29.14405516699182 34.0 29.0 35.0 6.0 35.0 92-93 28.68793047742419 34.0 29.0 35.0 2.0 35.0 94-95 28.206370578629183 33.0 29.0 35.0 2.0 35.0 96-97 27.62343177954505 33.0 27.0 35.0 2.0 35.0 98-99 26.69984871955141 33.0 25.5 34.5 2.0 35.0 100-101 23.823877962163692 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28313878025326744 1101 2 0.249622972106728 1101 3 0.2085296952498723 1101 4 0.10153864336146512 1101 5 0.05548023610609931 1101 6 0.060732953710669335 1101 7 0.10231652591441787 1101 8 0.04558392878716688 1101 9 0.14816046338506794 1101 10-11 0.2037031160696543 1101 12-13 0.24303798021375655 1101 14-15 0.2933327401261039 1101 16-17 0.23976461920731396 1101 18-19 0.3108621322698042 1101 20-21 0.22054301346838656 1101 22-23 0.23738992770627476 1101 24-25 0.15600107388134887 1101 26-27 0.15867387762039442 1101 28-29 0.005205026944679503 1101 30-31 0.1361565628968222 1101 32-33 0.11143273897649664 1101 34-35 0.17441629658256375 1101 36-37 0.07491668519327987 1101 38-39 0.10339607472875656 1101 40-41 0.1548414218996612 1101 42-43 0.134276638143092 1101 44-45 0.08968890102330818 1101 46-47 0.02167912894472579 1101 48-49 0.07558877455370094 1101 50-51 0.08037160178434277 1101 52-53 0.03852473994330552 1101 54-55 -0.07925352107729822 1101 56-57 -0.09928244235538841 1101 58-59 -0.10562748421148171 1101 60-61 -0.013815801267753614 1101 62-63 -0.13577731315453434 1101 64-65 -0.06095468692444683 1101 66-67 -0.18238019938108607 1101 68-69 -0.0823407255114148 1101 70-71 -0.11215245413422537 1101 72-73 -0.13489542036422364 1101 74-75 -0.33242531678573783 1101 76-77 -0.20805067338440608 1101 78-79 -0.19068204077067108 1101 80-81 -0.20827763320304982 1101 82-83 -0.19802649594887356 1101 84-85 -0.27050380511084526 1101 86-87 -0.43697402383646633 1101 88-89 -0.2374364185097484 1101 90-91 -0.21898341582992487 1101 92-93 -0.31151427196419235 1101 94-95 -0.30652979021441595 1101 96-97 -0.4390876603613769 1101 98-99 -0.35051428456990763 1101 100-101 -0.2783183043782742 1102 1 0.29881727253465584 1102 2 0.21976537904807003 1102 3 0.1574225488399037 1102 4 0.09013093376353254 1102 5 0.07983355233894684 1102 6 0.08906551073527424 1102 7 0.14111615737695615 1102 8 0.1155591736808006 1102 9 0.14270987737269536 1102 10-11 0.1652362049509648 1102 12-13 0.24439253048307563 1102 14-15 0.31189066375901575 1102 16-17 0.26177064745379397 1102 18-19 0.2747807047666555 1102 20-21 0.24782154932223932 1102 22-23 0.23899317568283607 1102 24-25 0.14106631170609063 1102 26-27 0.14690788062460314 1102 28-29 0.10890747726014638 1102 30-31 0.25105563966275923 1102 32-33 0.24199643983391184 1102 34-35 0.1297548369176127 1102 36-37 0.12407151586345577 1102 38-39 0.1783542609740536 1102 40-41 0.26311940643485343 1102 42-43 0.3336971027569007 1102 44-45 0.3215716216925486 1102 46-47 0.2863300580824557 1102 48-49 0.2624523292295464 1102 50-51 0.28575128183357634 1102 52-53 0.3817072385045819 1102 54-55 0.2782051943285353 1102 56-57 0.352188848632089 1102 58-59 0.18848691797346362 1102 60-61 0.30737337509812335 1102 62-63 0.24302179612029562 1102 64-65 0.21148582628845247 1102 66-67 0.2535519010314289 1102 68-69 0.27731652411196706 1102 70-71 0.10282749974590644 1102 72-73 0.013629019725037494 1102 74-75 0.056827812234296005 1102 76-77 0.03601711153871179 1102 78-79 0.16536417237674428 1102 80-81 0.12835334650477037 1102 82-83 0.15843026210529132 1102 84-85 0.2592662401567871 1102 86-87 0.08819929754698208 1102 88-89 0.15211491533120736 1102 90-91 0.11421290133666417 1102 92-93 0.15318181014044896 1102 94-95 0.08730991334411442 1102 96-97 0.02820460112667078 1102 98-99 0.07608045655049622 1102 100-101 0.198108174536344 1103 1 0.3374623411863098 1103 2 0.2145408263461306 1103 3 0.23245033671642545 1103 4 0.1159963345471482 1103 5 0.12285698947474799 1103 6 0.14701181575567546 1103 7 0.11375155913908941 1103 8 0.14868598633429286 1103 9 0.15444400916594248 1103 10-11 0.24410663577587144 1103 12-13 0.26046615826538044 1103 14-15 0.32323346420803034 1103 16-17 0.27142275500579416 1103 18-19 0.26205510094351325 1103 20-21 0.22641844288860113 1103 22-23 0.24129871677084225 1103 24-25 0.2094504774047934 1103 26-27 0.14078043603242207 1103 28-29 0.15614105880652573 1103 30-31 0.24226658341694218 1103 32-33 0.28558926221386827 1103 34-35 0.2533741000410643 1103 36-37 0.3128221657862582 1103 38-39 0.2586031387727772 1103 40-41 0.25148662610867945 1103 42-43 0.33881780930098415 1103 44-45 0.3753090854990617 1103 46-47 0.41096071352783525 1103 48-49 0.3239037492596637 1103 50-51 0.2622447042648588 1103 52-53 0.2789079199200515 1103 54-55 0.175735621736834 1103 56-57 0.2662413896496503 1103 58-59 0.2148694271974847 1103 60-61 0.2504589412045277 1103 62-63 0.1831915527531507 1103 64-65 0.25209816340562696 1103 66-67 0.3603040757688021 1103 68-69 0.3628538112496926 1103 70-71 0.3862667072593453 1103 72-73 0.2576699109090086 1103 74-75 0.2707672432273469 1103 76-77 0.2992371854143272 1103 78-79 0.31267424784449105 1103 80-81 0.4334465071945779 1103 82-83 0.45504761779562486 1103 84-85 0.41887447093847285 1103 86-87 0.33724844801913534 1103 88-89 0.3867881681801073 1103 90-91 0.2711923237867069 1103 92-93 0.3176612039201636 1103 94-95 0.16212494505498398 1103 96-97 0.1388908659847914 1103 98-99 0.20590762735222157 1103 100-101 0.40514092379430267 1104 1 0.31270438856221716 1104 2 0.19036575315535487 1104 3 0.16493892112681152 1104 4 0.0877733718631788 1104 5 0.05777798031625281 1104 6 0.057961443820609304 1104 7 0.09778229829712615 1104 8 0.09244109489335273 1104 9 0.11564892206635591 1104 10-11 0.16358251118420242 1104 12-13 0.16131301368288575 1104 14-15 0.2023123749312674 1104 16-17 0.14187055213044886 1104 18-19 0.16906746765259584 1104 20-21 0.14314288946167153 1104 22-23 0.07430829428287211 1104 24-25 0.03930284088423974 1104 26-27 0.151269403477869 1104 28-29 0.18943544429473036 1104 30-31 0.12470686466816261 1104 32-33 0.13737989905871473 1104 34-35 0.22475921785036945 1104 36-37 0.22133518682210962 1104 38-39 0.27001965772461034 1104 40-41 0.22669373045733465 1104 42-43 0.26362766347258315 1104 44-45 0.24040323911425077 1104 46-47 0.26177212779407455 1104 48-49 0.19435258724044502 1104 50-51 0.21582434232349357 1104 52-53 0.19326430727496557 1104 54-55 -0.0027093852986084244 1104 56-57 0.144617350750174 1104 58-59 0.1408510251584829 1104 60-61 0.207451992597683 1104 62-63 0.1250463182311634 1104 64-65 0.06783068152061844 1104 66-67 0.054779977198535335 1104 68-69 0.012753599108343394 1104 70-71 -0.0018235269377413488 1104 72-73 0.07583044898999702 1104 74-75 -0.04765834143268677 1104 76-77 0.06288570881864786 1104 78-79 0.0837516216693075 1104 80-81 -0.009335921310128725 1104 82-83 -0.007521448584675738 1104 84-85 0.06576440025393282 1104 86-87 0.09360439880924076 1104 88-89 0.10186264961442859 1104 90-91 0.21831356107320943 1104 92-93 0.24370306001645403 1104 94-95 0.11860548632396473 1104 96-97 0.13573703473463183 1104 98-99 0.157450161268887 1104 100-101 0.12490981507286492 1105 1 0.24463096504824478 1105 2 0.19472362996891945 1105 3 0.16325313344545123 1105 4 0.03564638684467525 1105 5 -0.004853270348867511 1105 6 0.09040940174944723 1105 7 0.10578792374792556 1105 8 0.11677989010684797 1105 9 0.18992327438571266 1105 10-11 0.15501312126330902 1105 12-13 0.21083952099626657 1105 14-15 0.250646356142326 1105 16-17 0.1726241866505589 1105 18-19 0.20812000930398256 1105 20-21 0.1159994981100283 1105 22-23 0.20650147368243665 1105 24-25 0.18345043580448106 1105 26-27 0.1238252663031858 1105 28-29 0.08435639089581315 1105 30-31 0.18444513192289236 1105 32-33 0.1839767304193458 1105 34-35 0.20536534317701438 1105 36-37 0.18058178139944658 1105 38-39 0.24128374352233806 1105 40-41 0.30220749995278595 1105 42-43 0.30843406135128504 1105 44-45 0.275257976553803 1105 46-47 0.2411672144668131 1105 48-49 0.2937419686477867 1105 50-51 0.4080523593151355 1105 52-53 0.4612297963315015 1105 54-55 0.3691333563031236 1105 56-57 0.38694140391260134 1105 58-59 0.3980695900329607 1105 60-61 0.3053716399945472 1105 62-63 0.2505099079959834 1105 64-65 0.3340184483082851 1105 66-67 0.22228110051504757 1105 68-69 0.46123043366155514 1105 70-71 0.20922621233458472 1105 72-73 0.1263523847008159 1105 74-75 0.1923459153456335 1105 76-77 0.20108334748911005 1105 78-79 0.2400560138368384 1105 80-81 0.18808835828926007 1105 82-83 0.20474638702090786 1105 84-85 0.1960843385306461 1105 86-87 0.11715631820241512 1105 88-89 0.05240937958096836 1105 90-91 0.18878278959125794 1105 92-93 0.33015734103476646 1105 94-95 0.28557369942301847 1105 96-97 0.33753751894316864 1105 98-99 0.33376920828263934 1105 100-101 0.196959926813296 1106 1 0.13696076723721973 1106 2 0.1350464535455842 1106 3 0.11974914208085607 1106 4 0.02563094854765069 1106 5 0.08644229145453153 1106 6 0.01783371643967513 1106 7 0.05427484512557612 1106 8 -0.03291699211024479 1106 9 0.04942410278214027 1106 10-11 -0.04325634605269357 1106 12-13 -0.0671182307860434 1106 14-15 0.076532175501697 1106 16-17 0.06196015160687551 1106 18-19 0.0987302613373231 1106 20-21 0.22643541035021997 1106 22-23 0.21809180654717863 1106 24-25 0.12709625867827157 1106 26-27 0.18695189799311862 1106 28-29 0.1506233981254539 1106 30-31 0.15388860014259365 1106 32-33 0.18116739966324502 1106 34-35 -0.1975180411989257 1106 36-37 0.17559320322894933 1106 38-39 0.19860501071784853 1106 40-41 0.07853042394113174 1106 42-43 0.03541876112034004 1106 44-45 0.12612904794651314 1106 46-47 0.16236443557335178 1106 48-49 0.15799070095022927 1106 50-51 0.3322110976422792 1106 52-53 0.3826341165443665 1106 54-55 0.23774340751307932 1106 56-57 0.26040008450302565 1106 58-59 0.3563340995218738 1106 60-61 0.4394250088066798 1106 62-63 0.280321510566516 1106 64-65 0.04862175938369262 1106 66-67 0.02340194598145473 1106 68-69 0.3780748080967413 1106 70-71 0.2953543376483765 1106 72-73 0.3258675845200756 1106 74-75 0.25811180549525403 1106 76-77 0.26156381741581214 1106 78-79 0.20565936256663164 1106 80-81 0.022126037775151985 1106 82-83 -0.10865888710300098 1106 84-85 -0.1595512674538888 1106 86-87 0.04997117160134934 1106 88-89 0.10565895283129478 1106 90-91 -0.17955018811734647 1106 92-93 -0.13440737429809602 1106 94-95 -0.02875442087018598 1106 96-97 0.03341875701928032 1106 98-99 0.0663298945965245 1106 100-101 -0.10351200811992811 1107 1 0.20662874483120675 1107 2 0.2056074425683576 1107 3 0.15238252935645136 1107 4 0.10501436704080902 1107 5 0.12288311746487324 1107 6 0.12458011506837607 1107 7 0.13838756637609606 1107 8 0.09904026695009804 1107 9 0.17773300653492896 1107 10-11 0.18315563367040255 1107 12-13 0.1967341675424592 1107 14-15 0.16576260587518732 1107 16-17 0.14163383034728128 1107 18-19 0.19949707814258488 1107 20-21 0.19623912126677823 1107 22-23 0.2220815053828602 1107 24-25 0.1614176015252866 1107 26-27 0.16955780435556989 1107 28-29 0.23270540129639983 1107 30-31 0.29601103793766725 1107 32-33 0.18055802657492848 1107 34-35 0.18061593384972952 1107 36-37 0.22989953957388565 1107 38-39 0.26858350844053547 1107 40-41 0.29429248558668064 1107 42-43 0.3048789685058466 1107 44-45 0.21355256492318375 1107 46-47 0.022594193665632645 1107 48-49 -0.019946871496593133 1107 50-51 0.1291243115626557 1107 52-53 0.12170095428746919 1107 54-55 0.07145597639667756 1107 56-57 0.15792631356045916 1107 58-59 0.22732792475145658 1107 60-61 0.27798134483628445 1107 62-63 0.24314297260785622 1107 64-65 0.28687630989496427 1107 66-67 0.16932922104788872 1107 68-69 0.24091477994417687 1107 70-71 0.1512853745885181 1107 72-73 0.2507065775866266 1107 74-75 0.17938198496783997 1107 76-77 0.14881237415546167 1107 78-79 0.05057280793841912 1107 80-81 0.10245906923362114 1107 82-83 0.12567675590937455 1107 84-85 0.20820381470189986 1107 86-87 0.15849917848308692 1107 88-89 0.05651589063456086 1107 90-91 0.19492912504586712 1107 92-93 0.23366516522207448 1107 94-95 0.13861774164276142 1107 96-97 0.2122993205246253 1107 98-99 0.09607100642098132 1107 100-101 0.2860998598506015 1108 1 0.17864475806990043 1108 2 0.07394396251950042 1108 3 0.07194389342552654 1108 4 0.026407701154781194 1108 5 0.027710061324086155 1108 6 0.032093709963589845 1108 7 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0.1083830545273905 2316 100-101 0.24539997678572334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 17.0 5 33.0 6 104.0 7 279.0 8 738.0 9 1432.0 10 2544.0 11 4139.0 12 6589.0 13 9359.0 14 12500.0 15 17071.0 16 23090.0 17 31685.0 18 29151.0 19 18346.0 20 15849.0 21 16217.0 22 16884.0 23 18326.0 24 21724.0 25 26429.0 26 31916.0 27 40428.0 28 50264.0 29 64062.0 30 80115.0 31 102785.0 32 132515.0 33 175873.0 34 243192.0 35 353695.0 36 554527.0 37 848970.0 38 640850.0 39 51194.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.223106287737515 19.542103482888663 15.46549069479862 22.7692995345752 2 15.37542997988143 20.15848349516855 40.70522876947314 23.760857755476884 3 19.340866336545332 23.917738133570946 30.12613841213999 26.615257117743734 4 12.901276811378178 16.914779229641855 37.1433167449147 33.04062721406527 5 13.63719121126934 37.470297487839574 34.514312265156896 14.378199035734184 6 32.40218034902384 36.45070977733967 17.09913769320786 14.04797218042863 7 28.681658556376604 32.902330915755925 20.281462339238786 18.134548188628685 8 26.2139013857904 35.92426150243364 19.29087873050696 18.570958381269005 9 25.90648172624964 17.127384396455245 20.28827009667839 36.67786378061672 10-11 24.798574104224024 27.142432834472945 27.13301726672382 20.925975794579212 12-13 25.572998649927005 24.088028163214435 28.04543305828593 22.293540128572626 14-15 22.518167516401068 25.57863879439008 26.366581816754387 25.53661187245447 16-17 21.841042706874163 28.99607812780082 27.02855387585121 22.134325289473807 18-19 21.71058356481081 27.460983726440148 29.555343636654612 21.273089072094425 20-21 22.346189289146594 26.476356905467973 29.95650721416409 21.22094659122134 22-23 22.12821390542266 26.327349150301643 29.794426444344463 21.750010499931236 24-25 21.962247328326416 26.464477628984312 29.974708298579607 21.59856674410966 26-27 21.729530376825398 26.815304152566625 29.82337403158364 21.63179143902434 28-29 21.668332709053548 26.99105333179425 29.836176997524888 21.504436961627313 30-31 21.818450834403222 26.70831476984152 29.772121299963246 21.70111309579201 32-33 21.739502808611704 26.60673369562681 29.754758773428776 21.89900472233271 34-35 22.10525506718131 26.720804808692407 29.686269439510195 21.487670684616088 36-37 22.069939825463376 26.59432600868044 29.621980052721693 21.7137541131345 38-39 21.834166322847466 26.839954289202872 29.72695128033879 21.59892810761088 40-41 22.234276162200587 26.683881962219775 29.473361013779055 21.608480861800587 42-43 21.986645136068837 26.65489017638106 29.42669841716064 21.93176627038946 44-45 22.0628575616754 26.94014910635831 29.27095505974219 21.726038272224102 46-47 22.09929827942166 27.168785443916573 28.80942753622592 21.922488740435856 48-49 22.226957456731622 27.203579123572602 28.69033295698513 21.87913046271065 50-51 22.32536798262265 27.428935110267826 28.446955628519344 21.798741278590178 52-53 22.383028041097994 27.40652166692333 28.263393233363608 21.947057058615066 54-55 22.29326603897087 27.443640033539207 28.35678602805649 21.906307899433433 56-57 22.20934787448362 27.647332361031847 28.35583324617757 21.787486518306963 58-59 22.294760524779274 27.3906689253049 28.36434294297707 21.950227606938753 60-61 22.237004389082003 27.44758781663964 28.41773442773524 21.89767336654311 62-63 22.16320335732244 27.396708064969065 28.61170061349424 21.828387964214254 64-65 22.22101744419457 27.287622987439274 28.49266484623182 21.998694722134342 66-67 22.126419506640723 27.405135409864055 28.368515439472308 22.09992964402291 68-69 22.224390822374353 27.368310383230078 28.42410297501745 21.983195819378118 70-71 22.25676884616472 27.228119991116973 28.350068612861772 22.16504254985653 72-73 22.26795497986084 27.22456513390557 28.251218876813354 22.256261009420232 74-75 22.170052290714303 27.552229636638693 27.93578363190016 22.341934440746844 76-77 22.29530953747602 27.43393112580818 28.151120136879847 22.119639199835955 78-79 22.313728913451715 27.44285258213024 28.09028953008082 22.153128974337225 80-81 22.20829102504239 27.544090523409494 28.01585713372 22.231761317828116 82-83 22.34953114815345 27.52501208862143 28.01865881977996 22.10679794344515 84-85 22.284562613937293 27.46431897857286 27.9472030959924 22.30391531149745 86-87 22.287019445755213 27.44655841783325 28.013057168966622 22.25336496744492 88-89 22.34187953947717 27.363039861341353 28.034633367948587 22.260447231232895 90-91 22.345256314186987 27.332666855454786 28.059620074665105 22.262456755693123 92-93 22.226521360962444 27.228074403862802 28.086921539558947 22.458482695615807 94-95 22.05099888742577 27.401937410905536 28.095916910222424 22.451146791446273 96-97 22.147460418243362 27.253223596675507 28.07082702998131 22.52848895509982 98-99 21.982691354035833 27.108195027453007 28.038425287373673 22.870688331137487 100-101 22.08287259825755 27.139804696374725 28.20622536311415 22.571097342253577 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 180.0 2 186.5 3 315.0 4 300.5 5 275.5 6 518.5 7 1285.5 8 2280.5 9 2937.5 10 2982.5 11 2907.5 12 3178.0 13 3479.5 14 3912.5 15 4337.0 16 4635.5 17 4933.5 18 5269.0 19 5798.0 20 6522.0 21 7486.0 22 8810.0 23 10902.0 24 13568.0 25 16963.5 26 22122.5 27 28099.0 28 32855.5 29 38047.5 30 45047.5 31 52518.0 32 59994.5 33 67023.5 34 75042.5 35 82518.0 36 91183.5 37 100290.5 38 105913.5 39 109897.5 40 114149.0 41 120401.0 42 124913.5 43 128469.0 44 133306.5 45 135557.5 46 137321.0 47 142347.0 48 145963.0 49 144033.5 50 140238.0 51 134441.5 52 126780.0 53 122606.0 54 120636.0 55 110637.5 56 95518.5 57 84031.0 58 75566.0 59 65786.5 60 53001.0 61 41784.5 62 32671.0 63 24619.0 64 17756.5 65 12502.5 66 8856.0 67 6723.0 68 5157.0 69 3806.0 70 2724.0 71 1777.5 72 1242.0 73 932.0 74 662.5 75 455.5 76 324.0 77 196.5 78 120.5 79 83.5 80 47.0 81 35.5 82 25.0 83 19.5 84 16.0 85 8.0 86 5.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017540955660911094 2 0.0 3 0.0 4 0.0 5 0.037772073535858625 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003760736972683599 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.039139664163443E-4 24-25 2.3333039611540577E-4 26-27 9.744975367172829E-4 28-29 0.0010568494412286026 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.078367444864714E-4 42-43 0.009195962670430697 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 6.86265870927664E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.3725317418553281E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.980342051380452E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010197910841985087 92-93 0.0014960595986223074 94-95 0.0 96-97 0.0 98-99 0.0011392013457399222 100-101 0.017170372090610155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3642903.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66330064851059 #Duplication Level Percentage of deduplicated Percentage of total 1 68.7433046013228 32.76532794784991 2 15.873027498307602 15.131217637078223 3 6.331188866242939 9.052960751827202 4 3.0368147550350897 5.789784587322821 5 1.695972127871752 4.041781471112279 6 1.0379523356758047 2.9683340540483805 7 0.6686627663513965 2.230947212554999 8 0.4754670154767764 1.8129861845695705 9 0.33993717367120757 1.4582274939266155 >10 1.6202157092840466 13.665369399966302 >50 0.11350814548717744 3.723706233978504 >100 0.0606073538122435 5.194821501816253 >500 0.002707889984327877 0.861425938217061 >1k 4.6091743771014724E-4 0.34194509433616005 >5k 5.7614679713768324E-5 0.2232858626764628 >10k+ 1.1522935942753665E-4 0.7378786287192911 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14651 0.40217925099844826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12219 0.33541930707460504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8131 0.22320111186051342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.745063483710656E-5 0.0 10-11 0.0 0.0 0.0 2.745063483710656E-5 0.0 12-13 0.0 0.0 0.0 2.745063483710656E-5 0.0 14-15 0.0 0.0 0.0 2.745063483710656E-5 0.0 16-17 0.0 0.0 0.0 4.1175952255659835E-5 0.0 18-19 0.0 0.0 0.0 5.490126967421312E-5 0.0 20-21 0.0 0.0 0.0 8.235190451131967E-5 0.0 22-23 0.0 0.0 0.0 1.372531741855328E-4 0.0 24-25 0.0 0.0 0.0 1.7842912644119266E-4 0.0 26-27 0.0 0.0 0.0 3.0195698320817214E-4 0.0 28-29 0.0 0.0 0.0 0.0010156734889729428 0.0 30-31 0.0 0.0 0.0 0.002154874834712865 0.0 32-33 0.0 0.0 0.0 0.0033078014978713405 0.0 34-35 0.0 0.0 0.0 0.004776410461656541 0.0 36-37 0.0 0.0 0.0 0.006684229582835448 0.0 38-39 1.372531741855328E-5 0.0 0.0 0.00896263227431529 0.0 40-41 2.745063483710656E-5 0.0 0.0 0.011913575519304248 0.0 42-43 2.745063483710656E-5 0.0 0.0 0.016195874553892868 0.0 44-45 2.745063483710656E-5 0.0 0.0 0.022413443344497505 0.0 46-47 2.745063483710656E-5 0.0 0.0 0.03538386830503036 0.0 48-49 2.745063483710656E-5 0.0 0.0 0.05944434973975425 0.0 50-51 2.745063483710656E-5 0.0 0.0 0.07802842952447539 0.0 52-53 2.745063483710656E-5 0.0 0.0 0.09692819160982327 0.0 54-55 2.745063483710656E-5 0.0 0.0 0.12477686065206788 0.0 56-57 2.745063483710656E-5 0.0 0.0 0.14738245844042513 0.0 58-59 5.490126967421312E-5 0.0 0.0 0.17326840709181662 0.0 60-61 5.490126967421312E-5 0.0 0.0 0.21482866823519595 0.0 62-63 6.86265870927664E-5 0.0 0.0 0.24728904393007445 0.0 64-65 8.235190451131968E-5 0.0 0.0 0.2821925261254554 0.0 66-67 8.235190451131968E-5 0.0 0.0 0.3427486265761125 0.0 68-69 8.235190451131968E-5 0.0 0.0 0.41171834660434276 0.0 70-71 8.235190451131968E-5 0.0 0.0 0.4721920951504885 0.0 72-73 1.0980253934842623E-4 0.0 0.0 0.5435088444572913 0.0 74-75 1.0980253934842623E-4 0.0 0.0 0.6136589417835172 0.0 76-77 1.0980253934842623E-4 0.0 0.0 0.6784836159513443 0.0 78-79 1.0980253934842623E-4 0.0 0.0 0.76068454197106 0.0 80-81 1.0980253934842623E-4 0.0 0.0 0.8825928112826502 0.0 82-83 1.0980253934842623E-4 0.0 0.0 0.9909404669847097 0.0 84-85 1.0980253934842623E-4 0.0 0.0 1.114756555417479 0.0 86-87 1.0980253934842623E-4 0.0 0.0 1.3115776071995329 0.0 88-89 1.0980253934842623E-4 0.0 0.0 1.5658253870608139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7920 0.0 49.622 1 GTATCAA 13530 0.0 40.04064 1 ATCAACG 17250 0.0 31.155708 3 TCAACGC 17545 0.0 30.63186 4 TATCAAC 17680 0.0 30.505472 2 CAACGCA 17785 0.0 30.218498 5 AACGCAG 18365 0.0 29.328476 6 CGCAGAG 21005 0.0 25.3964 8 ACGCAGA 21115 0.0 25.354082 7 GCAGAGT 23150 0.0 22.653387 9 GAGTACT 13995 0.0 22.283165 12-13 GTACATG 12925 0.0 21.288347 1 TACATGG 12510 0.0 21.157387 2 AGTACTT 14735 0.0 20.696646 12-13 GTACTTT 14945 0.0 20.626053 14-15 TGGTATC 4065 0.0 20.573883 2 ACATGGG 12645 0.0 20.555717 3 AGAGTAC 21480 0.0 20.31234 10-11 CAGAGTA 22180 0.0 20.121033 10-11 GGACCGA 965 0.0 19.195732 6 >>END_MODULE