FastQCFastQC Report
Thu 26 May 2016
SRR937590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639543
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115870.3183641462678144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88250.24247549761055165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57560.15815172399391902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48050.13202206980381878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65500.049.7948531
GTATCAA117000.042.2212751
ATCAACG147250.033.049643
TCAACGC149800.032.4602434
TATCAAC152450.031.9552692
CAACGCA153050.031.739465
AACGCAG158400.030.8317746
ACGCAGA182150.026.5519967
CGCAGAG181550.026.3780568
GTACATG132400.024.4546131
TACATGG128200.024.2086852
GCAGAGT198400.023.4912349
ACATGGG129700.022.8647633
GAGTACT112350.022.21945212-13
AGAGTAC181550.020.5142910-11
GTACTTT121050.020.48403414-15
AGTACTT121100.020.30017912-13
CAGAGTA189650.020.20171410-11
GGACCGA11150.019.5994366
TGGTATC33850.018.9548852
Produced by FastQC (version 0.11.5)