FastQCFastQC Report
Thu 26 May 2016
SRR937590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639543
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143910.39540678596186385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118090.32446381317654444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81270.22329726561823834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73600.051.5810361
GTATCAA126450.039.717071
ATCAACG162250.030.6565553
TCAACGC166350.029.929534
TATCAAC167250.029.8252812
CAACGCA169300.029.4080145
AACGCAG175100.028.4819076
ACGCAGA203400.024.3803167
CGCAGAG203000.024.3113638
GGACCGA12850.023.2864516
TACATGG121250.023.0386472
GTACATG127400.022.8618891
AGGACCG13150.022.3989335
ACATGGG122450.021.9205283
TGGTATC36650.021.776932
GAGTACT138100.021.65169112-13
GCAGAGT225050.021.6127859
GTACCGT2901.05501385E-1021.2917466
ACCGAGT13000.020.826748
AGTACTT144600.020.3006512-13
Produced by FastQC (version 0.11.5)