##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3639543 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23286440083274 33.0 31.0 34.0 31.0 34.0 2 32.4417428232061 34.0 31.0 34.0 31.0 34.0 3 32.54017661008539 34.0 31.0 34.0 31.0 34.0 4 36.15630478881552 37.0 35.0 37.0 35.0 37.0 5 36.0664962606569 37.0 35.0 37.0 35.0 37.0 6 36.096876173739396 37.0 35.0 37.0 35.0 37.0 7 36.04700727536397 37.0 35.0 37.0 35.0 37.0 8 36.02377111631873 37.0 35.0 37.0 35.0 37.0 9 37.71181299410393 39.0 37.0 39.0 35.0 39.0 10-11 37.64381352274172 39.0 37.0 39.0 35.0 39.0 12-13 37.54664720268451 39.0 37.0 39.0 35.0 39.0 14-15 38.79040321820624 40.0 38.0 41.0 35.0 41.0 16-17 38.75229939583074 40.0 38.0 41.0 35.0 41.0 18-19 38.73030776116672 40.0 38.0 41.0 35.0 41.0 20-21 38.61268598832326 40.0 38.0 41.0 34.0 41.0 22-23 38.45552697138075 40.0 38.0 41.0 34.0 41.0 24-25 38.54365644258084 40.0 38.0 41.0 34.0 41.0 26-27 38.43073457299447 40.0 38.0 41.0 34.0 41.0 28-29 38.29712947477197 40.0 38.0 41.0 34.0 41.0 30-31 38.357401327584256 40.0 38.0 41.0 34.0 41.0 32-33 38.30015087059007 40.0 38.0 41.0 34.0 41.0 34-35 38.1815738954039 40.0 38.0 41.0 34.0 41.0 36-37 38.02738461944261 40.0 38.0 41.0 33.0 41.0 38-39 37.89339980321705 40.0 37.0 41.0 33.0 41.0 40-41 37.72899592064168 40.0 37.0 41.0 33.0 41.0 42-43 37.526752809349965 40.0 37.0 41.0 33.0 41.0 44-45 37.2448071090244 40.0 37.0 41.0 32.0 41.0 46-47 36.93783862424486 40.0 36.5 41.0 31.0 41.0 48-49 36.642490829205755 39.5 36.0 41.0 31.0 41.0 50-51 36.40775050603881 39.0 36.0 41.0 30.0 41.0 52-53 36.16970592186986 39.0 35.5 40.5 30.0 41.0 54-55 35.95228769106451 39.0 35.0 40.0 29.0 41.0 56-57 35.693643267849836 39.0 35.0 40.0 28.5 41.0 58-59 35.427771426247745 38.0 35.0 40.0 28.0 41.0 60-61 35.08507620324859 38.0 34.0 40.0 27.5 41.0 62-63 34.73771885646083 38.0 34.0 40.0 27.0 41.0 64-65 34.34248626819356 37.0 34.0 40.0 26.0 41.0 66-67 33.90478694715243 36.5 33.0 39.0 26.0 40.5 68-69 33.46107766826769 36.0 33.0 39.0 25.5 40.0 70-71 33.04536009053884 35.5 32.0 38.5 24.5 40.0 72-73 32.539044325070485 35.0 32.0 38.0 23.5 40.0 74-75 32.0243023643353 35.0 31.0 37.0 22.0 39.0 76-77 30.775957860643494 33.5 29.5 36.0 21.0 38.5 78-79 31.385715596710906 34.0 31.0 36.0 22.0 38.5 80-81 31.189637682533217 34.0 31.0 36.0 20.5 37.5 82-83 30.82478541948811 34.0 31.0 35.0 20.0 37.0 84-85 30.42723042425931 34.0 30.5 35.0 18.0 36.5 86-87 29.996064890564558 34.0 30.0 35.0 16.0 36.0 88-89 29.60222863145181 34.0 29.5 35.0 9.0 36.0 90-91 29.05363242033409 34.0 29.0 35.0 4.5 35.0 92-93 28.554867053363566 33.5 29.0 35.0 2.0 35.0 94-95 28.069346893277533 33.0 28.5 35.0 2.0 35.0 96-97 27.496346794089256 33.0 27.0 35.0 2.0 35.0 98-99 26.57565537761197 32.5 25.5 34.5 2.0 35.0 100-101 23.837412966408145 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3123319620652012 1101 2 0.24241223342494322 1101 3 0.15541782743653698 1101 4 0.07739313831820027 1101 5 0.12221679136600017 1101 6 0.11393884507044305 1101 7 0.15591027937715296 1101 8 0.12335030269390046 1101 9 0.22494716517770996 1101 10-11 0.2087190154494607 1101 12-13 0.2432700233820171 1101 14-15 0.30553543152154106 1101 16-17 0.20602911565823234 1101 18-19 0.272598059694225 1101 20-21 0.31900347611199464 1101 22-23 0.2812742052167607 1101 24-25 0.29919147240189403 1101 26-27 0.15409691173958606 1101 28-29 0.22933902387588745 1101 30-31 0.24517094451120158 1101 32-33 0.3037147136792768 1101 34-35 0.23178813824175393 1101 36-37 0.191617941020084 1101 38-39 0.17748734877323358 1101 40-41 0.1406073393333358 1101 42-43 0.28598297405699213 1101 44-45 0.2799696504104432 1101 46-47 0.2127390041592747 1101 48-49 0.20443352253550273 1101 50-51 0.16609191411371427 1101 52-53 0.16860167709014462 1101 54-55 0.23202371516113374 1101 56-57 0.20706129424924313 1101 58-59 0.1581467721480081 1101 60-61 0.17033762613532843 1101 62-63 0.16998988327092945 1101 64-65 0.2497698727875175 1101 66-67 0.08068574508007487 1101 68-69 0.10893742793205519 1101 70-71 0.130108407306885 1101 72-73 0.08648704108892957 1101 74-75 0.035012427240566524 1101 76-77 -0.0330254704975772 1101 78-79 0.1237245029591314 1101 80-81 0.09873312857839878 1101 82-83 0.16193587749372185 1101 84-85 0.06664573622922276 1101 86-87 0.06392526660987485 1101 88-89 0.26711896110796474 1101 90-91 0.17038296427669763 1101 92-93 0.3758335853156858 1101 94-95 0.19510982878212602 1101 96-97 0.6021150222867462 1101 98-99 0.5637819586744968 1101 100-101 0.9634832356261178 1102 1 0.27785188928923077 1102 2 0.23090218029864928 1102 3 0.1877575603107502 1102 4 0.09383127163604144 1102 5 0.1325959635075833 1102 6 0.10356243252130781 1102 7 0.11691227083704092 1102 8 0.13755530481395795 1102 9 0.2124086119464792 1102 10-11 0.21148585182645974 1102 12-13 0.15735932268975716 1102 14-15 0.21691249988782602 1102 16-17 0.25400780387767696 1102 18-19 0.22916135018810024 1102 20-21 0.20630499759948862 1102 22-23 0.341776027444638 1102 24-25 0.16181282030550648 1102 26-27 0.14418304137944205 1102 28-29 0.18427736285236307 1102 30-31 0.22779299749068116 1102 32-33 0.22927105096604805 1102 34-35 0.23978598907115867 1102 36-37 0.19732125971975734 1102 38-39 0.20519786531788498 1102 40-41 0.19131029721629744 1102 42-43 0.31487304389813175 1102 44-45 0.2757076323508372 1102 46-47 0.1005568798884724 1102 48-49 0.2062526803713567 1102 50-51 0.18931585076340696 1102 52-53 0.216360356801772 1102 54-55 0.3626037663157007 1102 56-57 0.14789652905675865 1102 58-59 0.18669910236857135 1102 60-61 0.16212387169186826 1102 62-63 0.21237080720180046 1102 64-65 0.32109957688883384 1102 66-67 0.35376694181045565 1102 68-69 0.3768707084375862 1102 70-71 0.29262684310917564 1102 72-73 0.3743670007492099 1102 74-75 0.23354768386265334 1102 76-77 0.2149976954531354 1102 78-79 0.4684982901975907 1102 80-81 0.2589239516365005 1102 82-83 0.3563571681909856 1102 84-85 0.37985609844366763 1102 86-87 0.3741925579746841 1102 88-89 0.49021376262910366 1102 90-91 0.5361502475696973 1102 92-93 0.6258597324541171 1102 94-95 0.6269325796073453 1102 96-97 0.8254138611744288 1102 98-99 0.857199109569148 1102 100-101 1.2470597143502786 1103 1 0.2575072257362123 1103 2 0.23140355333099905 1103 3 0.2161014886968431 1103 4 0.14417980022106036 1103 5 0.11685474226558057 1103 6 0.17197741803419575 1103 7 0.16253951417729695 1103 8 0.15531418087346083 1103 9 0.21131991621909663 1103 10-11 0.16743151434160097 1103 12-13 0.1764286608232979 1103 14-15 0.28611711414141183 1103 16-17 0.33181562799980924 1103 18-19 0.19847171225781324 1103 20-21 0.23903776442183755 1103 22-23 0.2519080196389041 1103 24-25 0.18631448794518946 1103 26-27 0.19755447095477763 1103 28-29 0.2176027904384128 1103 30-31 0.2999172650265791 1103 32-33 0.2967378873697797 1103 34-35 0.3021708489569832 1103 36-37 0.2936017745528332 1103 38-39 0.21848452095704118 1103 40-41 0.19876237622511184 1103 42-43 0.3832501223663911 1103 44-45 0.3769912689405288 1103 46-47 0.4193428273889097 1103 48-49 0.23063164000204495 1103 50-51 0.304570294198804 1103 52-53 0.2020635223440692 1103 54-55 0.2875055863944098 1103 56-57 0.1955577117024916 1103 58-59 0.23872952800859792 1103 60-61 0.2115301973708128 1103 62-63 0.329437829018957 1103 64-65 0.3904787932873788 1103 66-67 0.33446441791753045 1103 68-69 0.3707865939894006 1103 70-71 0.3766847348017919 1103 72-73 0.2516490588922622 1103 74-75 0.24861495704203662 1103 76-77 0.2747117641696164 1103 78-79 0.3055916509915697 1103 80-81 0.28058421262076294 1103 82-83 0.3742935519264954 1103 84-85 0.3472304308472296 1103 86-87 0.5158933477385652 1103 88-89 0.4651736257583252 1103 90-91 0.5044463090210023 1103 92-93 0.470692346837442 1103 94-95 0.5822901761520605 1103 96-97 0.6015645190407142 1103 98-99 0.7220146151045235 1103 100-101 1.0269560071629442 1104 1 0.2159395511501785 1104 2 0.1670868236693721 1104 3 0.11363118632102953 1104 4 0.1021379760669987 1104 5 0.09678634773714379 1104 6 0.14677887141792212 1104 7 0.1870158685689418 1104 8 0.16631017619383925 1104 9 0.2211426304019639 1104 10-11 0.23005075480236314 1104 12-13 0.2705369446605914 1104 14-15 0.22400633300390638 1104 16-17 0.21936006194508906 1104 18-19 0.23656200878409805 1104 20-21 0.15877093576813195 1104 22-23 0.23482654240707745 1104 24-25 0.0802014457385738 1104 26-27 0.13652613447169415 1104 28-29 0.11214388340098225 1104 30-31 0.26187927681563394 1104 32-33 0.26781813844970515 1104 34-35 0.3136381815703757 1104 36-37 0.2697058737248952 1104 38-39 0.26511597794048924 1104 40-41 0.3438943281689646 1104 42-43 0.3087830484822973 1104 44-45 0.2840070514279276 1104 46-47 0.3629702121981211 1104 48-49 0.24565565683069934 1104 50-51 0.23292815057029514 1104 52-53 0.2903734575492507 1104 54-55 0.27787776287028265 1104 56-57 0.43891089969746133 1104 58-59 0.4508445275586368 1104 60-61 0.35245948552776696 1104 62-63 0.39560137968489784 1104 64-65 0.33177198983165823 1104 66-67 0.3169678488930501 1104 68-69 0.24962506816147112 1104 70-71 0.35451641451741267 1104 72-73 0.3911724907677083 1104 74-75 0.3897253557101408 1104 76-77 0.3507641174813543 1104 78-79 0.4412068642745055 1104 80-81 0.5967114849389752 1104 82-83 0.7183858057961849 1104 84-85 0.5605220862647968 1104 86-87 0.3420584732785201 1104 88-89 0.5556619491924479 1104 90-91 0.5787977984018511 1104 92-93 0.630711357816562 1104 94-95 0.5795316155869052 1104 96-97 0.7547331093034941 1104 98-99 0.8805470575091334 1104 100-101 1.1409054537072194 1105 1 0.19326729899628958 1105 2 0.23294315955810418 1105 3 0.17276357660892927 1105 4 0.14274035425037113 1105 5 0.09194631735715575 1105 6 0.11770486766789645 1105 7 0.1635497019062342 1105 8 0.10473611310789721 1105 9 0.26431900046818413 1105 10-11 0.22985138613646683 1105 12-13 0.2572694346328248 1105 14-15 0.2647457771450732 1105 16-17 0.23839472598515243 1105 18-19 0.24290709458382054 1105 20-21 0.3222680757771528 1105 22-23 0.20282520141545746 1105 24-25 0.17844332265215002 1105 26-27 0.11505246774964206 1105 28-29 0.22380843375344028 1105 30-31 0.25969719863463325 1105 32-33 0.29825746154561017 1105 34-35 0.2754881566492955 1105 36-37 0.23661631209861866 1105 38-39 0.2927261647299346 1105 40-41 0.32533346138995256 1105 42-43 0.3965556970000961 1105 44-45 0.3657947831346675 1105 46-47 0.13836182922041473 1105 48-49 0.1664303465038799 1105 50-51 0.24260257441857647 1105 52-53 0.20728330256385163 1105 54-55 0.12140124802444063 1105 56-57 0.18143368468784615 1105 58-59 0.2400845531917497 1105 60-61 0.2772954217297894 1105 62-63 0.2135481197698752 1105 64-65 0.2953551669137582 1105 66-67 0.32770646233144873 1105 68-69 0.13810144060112606 1105 70-71 0.35295677005819925 1105 72-73 0.36793654744537463 1105 74-75 0.27196374367207454 1105 76-77 0.23957840794250984 1105 78-79 0.35187427461794485 1105 80-81 0.30546115859145573 1105 82-83 0.3303446622744808 1105 84-85 0.4041600897300164 1105 86-87 0.4468385743869163 1105 88-89 0.4374864691649165 1105 90-91 0.5212360297169703 1105 92-93 0.6246558312696742 1105 94-95 0.7053599449249539 1105 96-97 0.8743652859821083 1105 98-99 0.9762098989873103 1105 100-101 1.0592963230970085 1106 1 0.08052738664930104 1106 2 0.056194956876694846 1106 3 0.10202802532327837 1106 4 0.028265732604822347 1106 5 0.03719340818381767 1106 6 0.05792756245836728 1106 7 0.08292619967172499 1106 8 0.01956642032523348 1106 9 7.646500757374497E-5 1106 10-11 -0.049411443371944586 1106 12-13 -0.09378655750862919 1106 14-15 0.08997300571218858 1106 16-17 0.16545030792776316 1106 18-19 0.11615808229944236 1106 20-21 0.042586681532888804 1106 22-23 0.09723678197634911 1106 24-25 0.1638504989728844 1106 26-27 0.1836636208401714 1106 28-29 0.19353252106102303 1106 30-31 0.20222348374441879 1106 32-33 0.2531301919586113 1106 34-35 0.09342733927413605 1106 36-37 -0.024969376279798894 1106 38-39 0.10632533569649638 1106 40-41 0.07882986675276271 1106 42-43 0.022829472340667678 1106 44-45 0.09160087782583304 1106 46-47 0.21741605103003536 1106 48-49 0.23163307108774234 1106 50-51 0.435425174215645 1106 52-53 0.21271465902664488 1106 54-55 0.25853395814339564 1106 56-57 0.2919968445142658 1106 58-59 0.21250758693600602 1106 60-61 0.30061279688900555 1106 62-63 0.2788852384468683 1106 64-65 0.3426626973008524 1106 66-67 0.32492061850926035 1106 68-69 0.2687829643818631 1106 70-71 0.39419956459175864 1106 72-73 0.25025535830052803 1106 74-75 0.1528026002967806 1106 76-77 0.16107822443250797 1106 78-79 0.28372432535305947 1106 80-81 0.19798096687747702 1106 82-83 -0.004187063367261601 1106 84-85 0.06485818338488514 1106 86-87 0.1953130125224547 1106 88-89 0.04479556478442248 1106 90-91 0.18648004405234175 1106 92-93 0.29179647549774756 1106 94-95 0.4425031798825323 1106 96-97 0.547343437496604 1106 98-99 0.8689456013988455 1106 100-101 1.1054517884567971 1107 1 0.2613820636091049 1107 2 0.2862890354720733 1107 3 0.22654559225864546 1107 4 0.10679944690868126 1107 5 0.13007433719724304 1107 6 0.16205749947189219 1107 7 0.18363778502933314 1107 8 0.21040699864259693 1107 9 0.29314484388546447 1107 10-11 0.27689568425080324 1107 12-13 0.2640333419317429 1107 14-15 0.38024156540351584 1107 16-17 0.27945332691729874 1107 18-19 0.31563820588497293 1107 20-21 0.34029892624963054 1107 22-23 0.2677437459059888 1107 24-25 0.3281621835577866 1107 26-27 0.3247202318921225 1107 28-29 0.4385623568731134 1107 30-31 0.3919875736292937 1107 32-33 0.36550636050657204 1107 34-35 0.38440015904378555 1107 36-37 0.34744551208151364 1107 38-39 0.4244147096480546 1107 40-41 0.45763345711407766 1107 42-43 0.4309711842365971 1107 44-45 0.42488583799420354 1107 46-47 0.39135547953896577 1107 48-49 0.3959007787953226 1107 50-51 0.44910832547686397 1107 52-53 0.4421563089349121 1107 54-55 0.37759272192019466 1107 56-57 0.2580692086969094 1107 58-59 0.19553469643371812 1107 60-61 0.34036411373816833 1107 62-63 0.3691281428596298 1107 64-65 0.4349022443499351 1107 66-67 0.4024921338583667 1107 68-69 0.3977964634770714 1107 70-71 0.43322465392530773 1107 72-73 0.5055183236458234 1107 74-75 0.4249750319946486 1107 76-77 0.3201295688438677 1107 78-79 0.3915635250562204 1107 80-81 0.5099818324701069 1107 82-83 0.50168878507775 1107 84-85 0.5458360208028346 1107 86-87 0.6640805250432642 1107 88-89 0.6244001391525131 1107 90-91 0.708570339376184 1107 92-93 0.7582658496559596 1107 94-95 0.9535143683225691 1107 96-97 1.0101576065020552 1107 98-99 1.20940034667192 1107 100-101 1.3664915597669562 1108 1 0.1460744373620102 1108 2 0.16313748839003495 1108 3 0.06114576662599802 1108 4 0.042298697814160846 1108 5 -0.0250162840741055 1108 6 0.011100232521023656 1108 7 0.10029851167956139 1108 8 0.007538011481997842 1108 9 0.1095431223953014 1108 10-11 0.06203017465040972 1108 12-13 0.08020150937007031 1108 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0.18012158090224517 2316 70-71 0.2376211294678896 2316 72-73 0.14064545992508926 2316 74-75 0.25614581796450864 2316 76-77 0.1812610885895367 2316 78-79 0.24291258689988737 2316 80-81 0.19082888933270326 2316 82-83 0.1786837171075959 2316 84-85 0.2804782113367601 2316 86-87 0.2756019375093608 2316 88-89 0.3889864971505119 2316 90-91 0.5558810324222456 2316 92-93 0.43384979944072555 2316 94-95 0.42796367820356096 2316 96-97 0.5912382686870075 2316 98-99 0.7590645231407436 2316 100-101 1.280841034752079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 6.0 4 26.0 5 58.0 6 98.0 7 306.0 8 723.0 9 1460.0 10 2622.0 11 4257.0 12 6810.0 13 9739.0 14 12869.0 15 17288.0 16 23032.0 17 31985.0 18 29250.0 19 18582.0 20 16245.0 21 16655.0 22 17741.0 23 19503.0 24 22451.0 25 27062.0 26 33177.0 27 41476.0 28 52501.0 29 65945.0 30 82865.0 31 105678.0 32 135665.0 33 180478.0 34 248443.0 35 356425.0 36 548929.0 37 822910.0 38 631792.0 39 54483.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20023765972228 19.59497265044454 15.471708379868618 22.733081309964557 2 15.342832877644252 20.193112157213143 40.74923692342692 23.714818041715677 3 19.342703191032502 23.97987879247477 30.095838955605142 26.581579060887588 4 12.920722695526715 16.931118511922243 37.12424176565122 33.02391702689982 5 13.635910175093763 37.503940303610186 34.49402928920083 14.36612023209522 6 32.40876670505061 36.43265102239484 17.10030627471636 14.05827599783819 7 28.65483386238327 32.88209536197264 20.263065994824075 18.20000478082001 8 26.192958841261117 35.942012499921006 19.30346749578175 18.561561163036128 9 25.92545272854312 17.13877264260925 20.306148326864115 36.62962630198352 10-11 24.794108709282654 27.149982058182047 27.129278903774157 20.92663032876115 12-13 25.597489158635963 24.12090671103274 28.03087307240788 22.250731057923417 14-15 22.478330383787196 25.60131862709137 26.389494505216724 25.530856483904714 16-17 21.813205668953493 29.004767906300323 27.02564580223396 22.156380622512224 18-19 21.681307241046472 27.523318174836785 29.54614082042718 21.249233763689563 20-21 22.335568275408242 26.480869504889693 29.97604781370186 21.207514406000204 22-23 22.143893585645756 26.347880991639254 29.76156948643347 21.746655936281513 24-25 21.944543854069572 26.520682201128302 29.922153677282243 21.612620267519887 26-27 21.762589087959654 26.87636642294412 29.77046979533845 21.590574693757777 28-29 21.6883356498174 26.96054988940088 29.82178436544296 21.529330095338764 30-31 21.815843362751863 26.75054258185712 29.72242668928489 21.71118736610613 32-33 21.72052919830869 26.621542869530597 29.788121750450536 21.869806181710178 34-35 22.12250274278941 26.709424782177322 29.66303736485597 21.505035110177296 36-37 22.06108019605758 26.575506869955927 29.62064193224259 21.7427710017439 38-39 21.861170993493136 26.857445249979257 29.681619280667732 21.599764475859875 40-41 22.21337948226971 26.69813235012689 29.45486912355625 21.633619044047148 42-43 21.97293187958036 26.68470896590613 29.43113309248233 21.91122606203118 44-45 22.04525706184225 26.957756366356485 29.275671324074388 21.721315247726878 46-47 22.109053252015432 27.16299821158865 28.808039910505244 21.91990862589067 48-49 22.221993255746668 27.19728823096746 28.667733833615923 21.912984679669947 50-51 22.34446742351993 27.442978417894775 28.390734770821503 21.821819387763792 52-53 22.385008227681332 27.44652281893633 28.240138940520833 21.928330012861505 54-55 22.31023235609526 27.403316844999498 28.32730647776383 21.95914432114142 56-57 22.235676292325714 27.63948660587332 28.324078050458535 21.800759051342435 58-59 22.254071458971637 27.42452829929472 28.35571663805044 21.96568360368321 60-61 22.276945209879372 27.46307709511881 28.38567919104129 21.874298503960524 62-63 22.188953393324383 27.4146232095623 28.577873650620422 21.818549746492895 64-65 22.199229408747197 27.31796272224287 28.475553112025327 22.007254756984597 66-67 22.161408726315365 27.408070189031974 28.340055331122617 22.090465753530044 68-69 22.231107650358204 27.354032457930145 28.44344150994823 21.97141838176342 70-71 22.247315424946954 27.264525692446234 28.314080684591538 22.174078198015273 72-73 22.273126049067148 27.242444999276007 28.216523338232303 22.267905613424542 74-75 22.18847256372572 27.531354898128697 27.93210576162996 22.348066776515623 76-77 22.288814831972037 27.407767953284246 28.141197947104896 22.16221926763882 78-79 22.342022061561025 27.415900842495883 28.064292687296177 22.17778440864691 80-81 22.186572127285814 27.535309049230516 28.005904040385303 22.272214783098367 82-83 22.27726825735222 27.492437791233304 28.064085546310334 22.166208405104136 84-85 22.264541518719327 27.524071122428822 27.922804254508897 22.288583104342955 86-87 22.293691817901312 27.433938821439945 27.994682299398576 22.277687061260163 88-89 22.352270601006772 27.378560989662716 28.00134522383717 22.267823185493345 90-91 22.287548616961818 27.33372408263221 28.0889803751523 22.28974692525367 92-93 22.182446845560154 27.217966484364258 28.131933109408514 22.46765356066708 94-95 22.071177617629466 27.363243132448222 28.111111752217244 22.454467497705068 96-97 22.13681772684098 27.259562532988348 28.071876056966495 22.531743683204184 98-99 21.98245549200416 27.08959079804253 28.045398712153485 22.88255499779983 100-101 22.039611992421882 27.126481698580175 28.22379192821563 22.610114380782314 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 182.0 2 203.5 3 294.5 4 277.0 5 297.0 6 556.5 7 1302.0 8 2285.0 9 2931.5 10 2949.0 11 2870.5 12 3160.5 13 3578.5 14 3948.5 15 4236.5 16 4547.5 17 4946.5 18 5349.0 19 5798.0 20 6490.0 21 7513.0 22 8971.0 23 11035.5 24 13694.0 25 17044.5 26 22040.5 27 28113.5 28 32861.0 29 37982.5 30 44755.0 31 51825.0 32 59552.0 33 67202.0 34 75208.0 35 82647.5 36 91357.5 37 100108.0 38 105986.5 39 109996.5 40 113774.0 41 120310.5 42 124465.0 43 127966.5 44 133310.0 45 135364.5 46 137537.5 47 142514.5 48 145448.5 49 143894.0 50 140061.0 51 133759.0 52 126618.0 53 122740.5 54 120412.0 55 110236.5 56 95834.0 57 84455.5 58 75488.0 59 65700.0 60 52730.5 61 41436.5 62 32485.5 63 24564.5 64 17801.5 65 12753.0 66 9093.5 67 6787.0 68 5170.5 69 3828.5 70 2827.0 71 1867.5 72 1256.5 73 931.0 74 631.0 75 425.0 76 300.0 77 179.5 78 120.0 79 86.5 80 45.5 81 30.0 82 30.0 83 22.5 84 14.5 85 7.5 86 5.5 87 4.0 88 1.0 89 1.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019975035327237513 2 0.0 3 0.0 4 5.4951954132702916E-5 5 0.023739244185327663 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.7475977066351458E-5 12-13 0.0034482351218271087 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.3737988533175729E-5 22-23 9.341832202559497E-4 24-25 1.3737988533175729E-5 26-27 6.044714954597321E-4 28-29 0.0013051089106516944 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.7475977066351458E-5 40-41 6.182094839929079E-4 42-43 0.013792940487308435 44-45 1.3737988533175729E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.7475977066351458E-5 70-71 1.3737988533175729E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.121396559952719E-5 82-83 0.0017584625322464933 84-85 4.3961563306162333E-4 86-87 0.0 88-89 0.0 90-91 0.010468347262279907 92-93 0.0021293882226422383 94-95 0.0 96-97 0.0 98-99 0.0015523927042488575 100-101 0.017722005207796692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3639543.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.65278554026061 #Duplication Level Percentage of deduplicated Percentage of total 1 68.52139173501612 32.652351852689094 2 16.190715761988912 15.430654118987498 3 6.259480426890711 8.94845035128246 4 2.9962200904203633 5.711129336008873 5 1.6821099647571052 4.007861270285284 6 1.063074525301977 3.039507742051768 7 0.6960546522341492 2.3218260147050214 8 0.47303651714943606 1.8033206163547109 9 0.3281336589038883 1.4072834588659213 >10 1.6107556751549883 13.597896270612994 >50 0.11503324380438178 3.755584893915425 >100 0.06081975297092255 5.211147963520919 >500 0.0025391963312276565 0.8267584825389448 >1k 4.616720551042994E-4 0.3422368689111962 >5k 5.770900688803733E-5 0.22349208787796607 >10k+ 1.1541801377607465E-4 0.720498671391991 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14391 0.39540678596186385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11809 0.32446381317654444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8127 0.22329726561823834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.747597706635146E-5 0.0 12-13 0.0 0.0 0.0 2.747597706635146E-5 0.0 14-15 0.0 0.0 0.0 2.747597706635146E-5 0.0 16-17 0.0 0.0 0.0 4.121396559952719E-5 0.0 18-19 0.0 0.0 0.0 5.495195413270292E-5 0.0 20-21 0.0 0.0 0.0 8.242793119905439E-5 0.0 22-23 0.0 0.0 0.0 1.236418967985816E-4 0.0 24-25 2.747597706635146E-5 0.0 0.0 1.3737988533175731E-4 0.0 26-27 2.747597706635146E-5 0.0 0.0 2.8849775919669035E-4 0.0 28-29 2.747597706635146E-5 0.0 0.0 7.281133922583138E-4 0.0 30-31 2.747597706635146E-5 0.0 0.0 0.0016897725895806148 0.0 32-33 2.747597706635146E-5 0.0 0.0 0.0030360954658318366 0.0 34-35 2.747597706635146E-5 0.0 0.0 0.004918199894876912 0.0 36-37 2.747597706635146E-5 0.0 0.0 0.006923946220720569 0.0 38-39 2.747597706635146E-5 0.0 0.0 0.009534164042023957 0.0 40-41 2.747597706635146E-5 0.0 0.0 0.012680163416121201 0.0 42-43 2.747597706635146E-5 0.0 0.0 0.01740603147153365 0.0 44-45 2.747597706635146E-5 0.0 0.0 0.025154257004244764 0.0 46-47 2.747597706635146E-5 0.0 0.0 0.04026604439073807 0.0 48-49 2.747597706635146E-5 0.0 0.0 0.06704138404189756 0.0 50-51 2.747597706635146E-5 0.0 0.0 0.08788191264672515 0.0 52-53 2.747597706635146E-5 0.0 0.0 0.10928569878141295 0.0 54-55 2.747597706635146E-5 0.0 0.0 0.1409655003389162 0.0 56-57 2.747597706635146E-5 1.373798853317573E-5 0.0 0.16463605458157796 0.0 58-59 5.495195413270292E-5 2.747597706635146E-5 0.0 0.19167241601486779 0.0 60-61 6.868994266587866E-5 5.495195413270292E-5 0.0 0.23460363018104197 0.0 62-63 8.242793119905439E-5 5.495195413270292E-5 0.0 0.26791825237399314 0.0 64-65 8.242793119905439E-5 5.495195413270292E-5 0.0 0.30492839348236855 0.0 66-67 8.242793119905439E-5 5.495195413270292E-5 0.0 0.36878256418456934 0.0 68-69 8.242793119905439E-5 5.495195413270292E-5 0.0 0.4405360782933462 0.0 70-71 8.242793119905439E-5 5.495195413270292E-5 0.0 0.5039094193968858 0.0 72-73 8.242793119905439E-5 5.495195413270292E-5 0.0 0.5795782602376178 0.0 74-75 8.242793119905439E-5 5.495195413270292E-5 0.0 0.6536672323970345 0.0 76-77 8.242793119905439E-5 5.495195413270292E-5 0.0 0.7220137253495837 0.0 78-79 8.242793119905439E-5 5.495195413270292E-5 0.0 0.8049362241358324 0.0 80-81 8.242793119905439E-5 5.495195413270292E-5 0.0 0.9293474482922719 0.0 82-83 8.242793119905439E-5 5.495195413270292E-5 0.0 1.0398695660416706 0.0 84-85 8.242793119905439E-5 5.495195413270292E-5 0.0 1.1622887818607994 0.0 86-87 8.242793119905439E-5 5.495195413270292E-5 0.0 1.363385458009426 0.0 88-89 8.242793119905439E-5 5.495195413270292E-5 0.0 1.6265778423280066 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7360 0.0 51.581036 1 GTATCAA 12645 0.0 39.71707 1 ATCAACG 16225 0.0 30.656555 3 TCAACGC 16635 0.0 29.92953 4 TATCAAC 16725 0.0 29.825281 2 CAACGCA 16930 0.0 29.408014 5 AACGCAG 17510 0.0 28.481907 6 ACGCAGA 20340 0.0 24.380316 7 CGCAGAG 20300 0.0 24.311363 8 GGACCGA 1285 0.0 23.286451 6 TACATGG 12125 0.0 23.038647 2 GTACATG 12740 0.0 22.861889 1 AGGACCG 1315 0.0 22.398933 5 ACATGGG 12245 0.0 21.920528 3 TGGTATC 3665 0.0 21.77693 2 GAGTACT 13810 0.0 21.651691 12-13 GCAGAGT 22505 0.0 21.612785 9 GTACCGT 290 1.05501385E-10 21.291746 6 ACCGAGT 1300 0.0 20.82674 8 AGTACTT 14460 0.0 20.30065 12-13 >>END_MODULE