FastQCFastQC Report
Thu 26 May 2016
SRR937589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485880
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113870.3266606997372256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86300.2475701974824148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57810.16584047643636612No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48030.13778443319907743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62550.048.3418771
GTATCAA112250.042.312911
ATCAACG139650.033.6564183
TCAACGC142400.032.9373364
CAACGCA144950.032.3588265
TATCAAC146500.032.280612
AACGCAG149650.031.4265126
ACGCAGA175050.026.5954847
CGCAGAG174450.026.578038
GTACATG126750.023.781271
GCAGAGT193100.023.4192879
TACATGG125000.023.1940252
ACATGGG125550.021.8409563
GAGTACT109150.021.60628712-13
TGGTATC33250.021.584482
AGTACTT115250.020.68937712-13
AGAGTAC178500.020.47705710-11
CAGAGTA184650.019.80790510-11
GTACTTT118550.019.67145314-15
GTGGTAT41000.018.9015541
Produced by FastQC (version 0.11.5)