##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937589_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3485880 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.708807532100934 33.0 31.0 34.0 30.0 34.0 2 31.84832983349972 33.0 31.0 34.0 30.0 34.0 3 31.877892526420876 33.0 31.0 34.0 30.0 34.0 4 35.47264277599918 37.0 35.0 37.0 33.0 37.0 5 35.462794760576955 37.0 35.0 37.0 33.0 37.0 6 35.508425992862634 37.0 35.0 37.0 33.0 37.0 7 35.44756704189473 37.0 35.0 37.0 33.0 37.0 8 35.42239405831526 37.0 35.0 37.0 33.0 37.0 9 37.047005634158374 39.0 37.0 39.0 33.0 39.0 10-11 36.93362163929912 39.0 37.0 39.0 33.0 39.0 12-13 36.808756038647346 39.0 37.0 39.0 33.0 39.0 14-15 37.96618113647057 40.0 38.0 41.0 33.0 41.0 16-17 37.89296633848555 40.0 38.0 41.0 33.0 41.0 18-19 37.816569704063255 40.0 38.0 41.0 33.0 41.0 20-21 37.722418872709326 40.0 37.5 41.0 32.0 41.0 22-23 37.52403568109057 40.0 37.0 41.0 32.0 41.0 24-25 37.35907833889864 40.0 37.0 41.0 32.0 41.0 26-27 37.307164905274995 39.5 37.0 41.0 32.0 41.0 28-29 37.35921302511848 40.0 37.0 41.0 32.0 41.0 30-31 37.19729006735746 39.5 37.0 41.0 31.0 41.0 32-33 36.97789109206283 39.0 36.0 41.0 31.0 41.0 34-35 37.01939065601799 39.0 36.0 41.0 31.0 41.0 36-37 36.941010017556536 40.0 36.0 41.0 31.0 41.0 38-39 36.81319336867591 39.5 36.0 41.0 30.0 41.0 40-41 36.59245513328055 39.0 36.0 41.0 30.0 41.0 42-43 36.35636897999932 39.0 35.5 41.0 30.0 41.0 44-45 36.150418975983115 39.0 35.0 41.0 29.5 41.0 46-47 35.85158840809207 39.0 35.0 40.5 28.5 41.0 48-49 35.53163964336122 39.0 35.0 40.0 27.0 41.0 50-51 34.614796694091595 38.0 34.0 39.5 25.0 40.5 52-53 34.71995550621364 38.0 34.0 39.5 26.0 40.5 54-55 35.08049975902785 38.5 35.0 40.0 26.0 41.0 56-57 34.88256437398878 38.0 34.0 40.0 26.0 41.0 58-59 34.63778701504354 38.0 34.0 40.0 25.0 41.0 60-61 34.31844842622236 38.0 34.0 40.0 24.0 41.0 62-63 33.95343299252987 37.5 33.5 40.0 23.0 41.0 64-65 33.57437418958771 37.0 33.0 40.0 22.0 41.0 66-67 33.16572630153648 36.5 32.5 39.0 21.5 41.0 68-69 32.6706098603509 36.0 32.0 39.0 19.5 40.0 70-71 32.2210988330063 35.0 31.5 38.5 18.0 40.0 72-73 31.755768701160108 35.0 31.0 38.0 16.5 40.0 74-75 31.286249670097654 35.0 31.0 37.0 12.0 39.0 76-77 30.780857487922702 35.0 30.0 37.0 7.5 39.0 78-79 30.242796223622157 34.0 30.0 36.0 7.0 38.5 80-81 29.709937806235445 34.0 29.0 36.0 2.0 37.5 82-83 29.172451002329396 34.0 29.0 35.0 2.0 37.0 84-85 28.69781188681194 34.0 28.5 35.0 2.0 36.5 86-87 28.20870856139626 34.0 27.5 35.0 2.0 36.0 88-89 27.71065455494739 33.0 26.5 35.0 2.0 36.0 90-91 27.19628702078098 33.0 26.0 35.0 2.0 35.0 92-93 26.601068022995626 32.5 25.0 35.0 2.0 35.0 94-95 25.944429957428255 32.0 23.5 34.5 2.0 35.0 96-97 25.19489770158468 32.0 19.5 34.0 2.0 35.0 98-99 24.23131605218768 31.5 9.0 34.0 2.0 35.0 100-101 21.83945546031418 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06386068565786474 1101 2 0.14323107568048954 1101 3 0.09902018592995532 1101 4 0.08193266214052386 1101 5 0.0716300286379905 1101 6 0.022263282039354237 1101 7 0.0483062832970802 1101 8 0.016447848910907226 1101 9 0.17249381124985774 1101 10-11 0.15740199207635186 1101 12-13 0.27036027784242833 1101 14-15 0.350762953019192 1101 16-17 0.30898557182668895 1101 18-19 0.32956034583450844 1101 20-21 0.34617298876503355 1101 22-23 0.41510067173953047 1101 24-25 0.25440820808899645 1101 26-27 0.31438633856785003 1101 28-29 0.16523824951519828 1101 30-31 0.314697440084295 1101 32-33 0.2187298942118474 1101 34-35 0.34347086333821153 1101 36-37 0.337100378654668 1101 38-39 0.39026534422161063 1101 40-41 0.3845823357627509 1101 42-43 0.35790633592592513 1101 44-45 0.41023070345266177 1101 46-47 0.45226242964628227 1101 48-49 0.5091592070509634 1101 50-51 0.3977462003004675 1101 52-53 0.33236157284203216 1101 54-55 0.39973629782866027 1101 56-57 0.3926530515935909 1101 58-59 0.49918378468755265 1101 60-61 0.4793555332550028 1101 62-63 0.5733826345791684 1101 64-65 0.41152074293857055 1101 66-67 0.6316445471325238 1101 68-69 0.5523576370943815 1101 70-71 0.5720456707321304 1101 72-73 0.5718040739720358 1101 74-75 0.5457809803083116 1101 76-77 0.7061592146597881 1101 78-79 0.779993339621555 1101 80-81 0.914468723426193 1101 82-83 0.9406028336881 1101 84-85 1.1022965627793297 1101 86-87 1.1262733446354574 1101 88-89 1.173297232106595 1101 90-91 1.3325210955853066 1101 92-93 1.3214894751840234 1101 94-95 1.3152417808134231 1101 96-97 1.5149785230855457 1101 98-99 1.8400667064093454 1101 100-101 2.2912244754563353 1102 1 0.22711053112969282 1102 2 0.18150786051642953 1102 3 0.282152703718463 1102 4 0.12397342345974494 1102 5 0.15126179383022986 1102 6 0.09101353437377213 1102 7 0.022713887391070386 1102 8 0.12028623206003175 1102 9 0.10673802205869976 1102 10-11 0.15512721511154126 1102 12-13 0.18776269479384666 1102 14-15 0.36191506788011196 1102 16-17 0.3733036235036451 1102 18-19 0.29026612021257137 1102 20-21 0.40007595530791207 1102 22-23 0.5114858071244086 1102 24-25 0.4922947346539175 1102 26-27 0.3674003771751586 1102 28-29 0.2801018695174875 1102 30-31 0.47872724767326247 1102 32-33 0.4566965280679369 1102 34-35 0.5517214594160649 1102 36-37 0.6088765541918448 1102 38-39 0.564591604230408 1102 40-41 0.5877619341771521 1102 42-43 0.5551209620530457 1102 44-45 0.6617709384947048 1102 46-47 0.6701080595554316 1102 48-49 0.7062121259132894 1102 50-51 0.6509505630274148 1102 52-53 0.6366754535377552 1102 54-55 0.7028620032988115 1102 56-57 0.6759781112013812 1102 58-59 0.6321946559648737 1102 60-61 0.8035581991574148 1102 62-63 0.8086617292345721 1102 64-65 0.7028424578003936 1102 66-67 0.8623220572131061 1102 68-69 0.7388474109401955 1102 70-71 0.74171859984137 1102 72-73 0.6165115480178436 1102 74-75 0.6991908498677724 1102 76-77 0.7534370167943507 1102 78-79 0.769704563854301 1102 80-81 0.8589791065252719 1102 82-83 0.724053207541079 1102 84-85 0.9628911109912686 1102 86-87 1.1525949514555052 1102 88-89 1.1339336322910576 1102 90-91 1.180160652864224 1102 92-93 1.2433641819785421 1102 94-95 1.4700550561908727 1102 96-97 1.5346882574790506 1102 98-99 1.7533795288642935 1102 100-101 2.112863068320234 1103 1 0.2982301426490608 1103 2 0.25695894485723514 1103 3 0.33984926069394916 1103 4 0.29987799859497244 1103 5 0.31032773065902575 1103 6 0.2707883334169381 1103 7 0.2180868158655187 1103 8 0.2764984022501764 1103 9 0.3618677191328814 1103 10-11 0.29487784186699884 1103 12-13 0.3814719018135051 1103 14-15 0.44511108968904267 1103 16-17 0.41375176263257885 1103 18-19 0.48878819852647837 1103 20-21 0.30399908527795105 1103 22-23 0.44538135267102774 1103 24-25 0.4059362377176541 1103 26-27 0.4790898871924725 1103 28-29 0.34985662675300233 1103 30-31 0.3210351803500373 1103 32-33 0.3960445228096532 1103 34-35 0.40346800597556154 1103 36-37 0.5019963596641901 1103 38-39 0.5549369123642194 1103 40-41 0.6073893648748765 1103 42-43 0.5480629413790936 1103 44-45 0.4337992197121494 1103 46-47 0.5167916791489944 1103 48-49 0.4268480402579158 1103 50-51 0.5247791588796957 1103 52-53 0.3985211675361242 1103 54-55 0.5327263080492273 1103 56-57 0.4817956230001883 1103 58-59 0.5136075315691784 1103 60-61 0.7302964847567637 1103 62-63 0.7559980005963354 1103 64-65 0.8111510441705363 1103 66-67 0.7463772188765034 1103 68-69 0.7380498331973797 1103 70-71 0.8022217282310606 1103 72-73 0.9036160814891012 1103 74-75 0.7875660626772891 1103 76-77 0.8153761873939551 1103 78-79 0.9199822398666306 1103 80-81 0.8357009061215663 1103 82-83 0.9772798308197466 1103 84-85 0.9111535078190585 1103 86-87 1.0006986300969736 1103 88-89 1.0175214031966782 1103 90-91 1.1517893909486858 1103 92-93 1.285983337349247 1103 94-95 1.4098625758196306 1103 96-97 1.834306493368974 1103 98-99 1.978638025082212 1103 100-101 2.4861249623069703 1104 1 0.1526310313497099 1104 2 0.23596725599163548 1104 3 0.21682351735455896 1104 4 0.24029753068962378 1104 5 0.15435861226344372 1104 6 0.12032611248268665 1104 7 0.1565143507243576 1104 8 0.18691418188808484 1104 9 0.39139026545343114 1104 10-11 0.3140918693676795 1104 12-13 0.3855378558849196 1104 14-15 0.5573820690802549 1104 16-17 0.6413688782936973 1104 18-19 0.5084834693163884 1104 20-21 0.5331421876308582 1104 22-23 0.6426289813784365 1104 24-25 0.5452294383403355 1104 26-27 0.6057701147176715 1104 28-29 0.5194518072610421 1104 30-31 0.5952968194677197 1104 32-33 0.5828319368468158 1104 34-35 0.5682986438560533 1104 36-37 0.6289236920389811 1104 38-39 0.6500661863567174 1104 40-41 0.7031480856215069 1104 42-43 0.7256290830733434 1104 44-45 0.7227154284676374 1104 46-47 0.6450150209160626 1104 48-49 0.6767839884219953 1104 50-51 0.7350741168410266 1104 52-53 0.6905489059936087 1104 54-55 0.6380808409055092 1104 56-57 0.7100020229823514 1104 58-59 0.7303897813341891 1104 60-61 0.8374778695990699 1104 62-63 0.9178615969360209 1104 64-65 0.7104070917561458 1104 66-67 0.7941759757358895 1104 68-69 0.8954465941956045 1104 70-71 0.8834044951663458 1104 72-73 0.8311465562677576 1104 74-75 0.887965643591027 1104 76-77 1.1023443138034459 1104 78-79 1.1245534009361897 1104 80-81 1.118921034150933 1104 82-83 1.2261618959341583 1104 84-85 1.1918375323846462 1104 86-87 1.4102758656880994 1104 88-89 1.542002430998295 1104 90-91 1.5081759237158074 1104 92-93 1.4957621653195403 1104 94-95 1.631228098544284 1104 96-97 1.96292328579867 1104 98-99 2.189374769807408 1104 100-101 2.6131830733722587 1105 1 0.24281705407462084 1105 2 0.18690806931110515 1105 3 0.17976535660420367 1105 4 0.20065330019772887 1105 5 0.15695522663186523 1105 6 0.11513210840563204 1105 7 0.14718762825180676 1105 8 0.16640395220439785 1105 9 0.2528839552695814 1105 10-11 0.3308462735326785 1105 12-13 0.23272768180864034 1105 14-15 0.31008854915664585 1105 16-17 0.3652256469051949 1105 18-19 0.40478838394987804 1105 20-21 0.34898384579204134 1105 22-23 0.46398059475286857 1105 24-25 0.39156569222517845 1105 26-27 0.46150062320749186 1105 28-29 0.5111571401440571 1105 30-31 0.4881813982920704 1105 32-33 0.44094630635100884 1105 34-35 0.4490779910257956 1105 36-37 0.4682001631617396 1105 38-39 0.484054992304884 1105 40-41 0.6352203746211984 1105 42-43 0.689139324232066 1105 44-45 0.6078525752962989 1105 46-47 0.6094126389468784 1105 48-49 0.620053663222599 1105 50-51 0.4999204862794784 1105 52-53 0.5291736261345577 1105 54-55 0.6420429069204587 1105 56-57 0.5427935485758653 1105 58-59 0.4852049541838852 1105 60-61 0.5446006254931319 1105 62-63 0.6002387497068824 1105 64-65 0.7110237973603972 1105 66-67 0.754981907770798 1105 68-69 0.7360667299049481 1105 70-71 0.9193550824245236 1105 72-73 0.982130028805674 1105 74-75 0.9494928436111287 1105 76-77 1.0441737166271103 1105 78-79 1.1378404836752232 1105 80-81 1.1305022133565537 1105 82-83 1.033227865911126 1105 84-85 1.0862469728030106 1105 86-87 1.1886357947413657 1105 88-89 1.1821105165251424 1105 90-91 1.3316890305319475 1105 92-93 1.4239543417646416 1105 94-95 1.4312228882580627 1105 96-97 1.6737265816768705 1105 98-99 1.7417282219786863 1105 100-101 2.0214934333751238 1106 1 0.3016846153472308 1106 2 0.2673041738579265 1106 3 0.19307006416685013 1106 4 0.22897084785589072 1106 5 0.23097987030276101 1106 6 0.17300667529914193 1106 7 0.20220822200373334 1106 8 0.10672964626293435 1106 9 0.21465974503414742 1106 10-11 0.1201963328997735 1106 12-13 0.12621258596595908 1106 14-15 0.05253430139796933 1106 16-17 0.031695182424734014 1106 18-19 0.33805784577931774 1106 20-21 0.40423699862510887 1106 22-23 0.3740374211590236 1106 24-25 0.30313984643387215 1106 26-27 0.2810466699055567 1106 28-29 0.3775723190905538 1106 30-31 0.4742860564580482 1106 32-33 0.5039857599105559 1106 34-35 0.2878874707311567 1106 36-37 0.3579572894906704 1106 38-39 0.474638247443707 1106 40-41 0.47475182501045055 1106 42-43 0.4285850896323282 1106 44-45 0.45285738843418955 1106 46-47 0.5466067566951551 1106 48-49 0.660851459807553 1106 50-51 0.495857408361303 1106 52-53 0.5507864372803866 1106 54-55 0.6349879399747209 1106 56-57 0.5375403451314611 1106 58-59 0.557352423465332 1106 60-61 0.5416327768493616 1106 62-63 0.7292154087717293 1106 64-65 0.652582889635994 1106 66-67 0.696102199777954 1106 68-69 0.7210144129686853 1106 70-71 0.8239208321150429 1106 72-73 0.7318767190774658 1106 74-75 1.0027369795288799 1106 76-77 1.1737696053672018 1106 78-79 1.2186963921942287 1106 80-81 1.1366859061821977 1106 82-83 0.7976667734755978 1106 84-85 0.9457815408504544 1106 86-87 1.1131100188949183 1106 88-89 1.2184950047585126 1106 90-91 1.4701661962162476 1106 92-93 1.49244640742576 1106 94-95 1.540557035421653 1106 96-97 1.5991234504548366 1106 98-99 1.9476573904040961 1106 100-101 2.525046349043482 1107 1 0.10284793562073347 1107 2 0.1436474815240203 1107 3 0.16658545093993027 1107 4 0.1212000976545653 1107 5 0.1108505387670391 1107 6 0.08051864178393942 1107 7 0.19709437828134924 1107 8 0.1847719383989883 1107 9 0.1987888835377305 1107 10-11 0.2870937332997059 1107 12-13 0.2584350796396251 1107 14-15 0.5005166630859108 1107 16-17 0.5058462180120173 1107 18-19 0.4466173132518918 1107 20-21 0.42137387952729455 1107 22-23 0.4530690030272737 1107 24-25 0.47860380220537735 1107 26-27 0.510327383883201 1107 28-29 0.43343778171922764 1107 30-31 0.36064969282168846 1107 32-33 0.40304050563594274 1107 34-35 0.4748737865619077 1107 36-37 0.34313302414386015 1107 38-39 0.46308648014395004 1107 40-41 0.47076148868210055 1107 42-43 0.5985725123817716 1107 44-45 0.6316481325429351 1107 46-47 0.5745262924528589 1107 48-49 0.453991127611296 1107 50-51 0.4329621071859222 1107 52-53 0.5599667745437387 1107 54-55 0.6493424435050983 1107 56-57 0.6363629998663285 1107 58-59 0.49540720236812774 1107 60-61 0.7146400969901521 1107 62-63 0.7385902862415605 1107 64-65 0.7823593041242631 1107 66-67 0.8511439580594882 1107 68-69 0.8058708905543313 1107 70-71 1.0427544353917568 1107 72-73 1.0566171059259553 1107 74-75 1.090183556375269 1107 76-77 1.1772502161932898 1107 78-79 1.3035890633066636 1107 80-81 1.1329398761596643 1107 82-83 1.2873342756095063 1107 84-85 1.3323555520885435 1107 86-87 1.586580163759507 1107 88-89 1.5733001478586708 1107 90-91 1.5965328788683877 1107 92-93 1.7013071860088473 1107 94-95 1.9081950458634864 1107 96-97 1.9232651360477035 1107 98-99 2.1881724242902862 1107 100-101 2.7274043573137057 1108 1 0.13506887814325808 1108 2 0.08864361872678472 1108 3 0.18047351039382775 1108 4 0.2430952855097388 1108 5 0.18077190833009382 1108 6 0.22732294726247204 1108 7 0.22448137368865417 1108 8 0.06815906651588222 1108 9 0.1930401881204773 1108 10-11 0.22502189949004503 1108 12-13 0.25900691581910706 1108 14-15 0.27900064551137405 1108 16-17 0.309001862672595 1108 18-19 0.33606310849046395 1108 20-21 0.42752449544201454 1108 22-23 0.44398479404758007 1108 24-25 0.3560682905387793 1108 26-27 0.4748842049195616 1108 28-29 0.4246905603264324 1108 30-31 0.4184461447357819 1108 32-33 0.3845519257708361 1108 34-35 0.449762523782681 1108 36-37 0.4218483004405016 1108 38-39 0.4262934549812698 1108 40-41 0.49058664378644323 1108 42-43 0.537654778113783 1108 44-45 0.5079248699722498 1108 46-47 0.4193807147632356 1108 48-49 0.5555675280530323 1108 50-51 0.5743269780073419 1108 52-53 0.5311021999490961 1108 54-55 0.6096250675530257 1108 56-57 0.6445007050329963 1108 58-59 0.6353042102406476 1108 60-61 0.6693681134082254 1108 62-63 0.7242412979152633 1108 64-65 0.8630998236823402 1108 66-67 0.8387301600529753 1108 68-69 0.8869213818168262 1108 70-71 0.9820016401958469 1108 72-73 0.9590942727656149 1108 74-75 1.1132963547941337 1108 76-77 1.1179212054011565 1108 78-79 1.3173892026517429 1108 80-81 1.3784820185665403 1108 82-83 1.3377440164940175 1108 84-85 1.3111495062184204 1108 86-87 1.4332356449028936 1108 88-89 1.512591431207884 1108 90-91 1.6153788267229956 1108 92-93 1.7299729331875824 1108 94-95 1.9630678579324758 1108 96-97 2.093818298090863 1108 98-99 2.2413591135175857 1108 100-101 2.777325442499045 1109 1 0.04617409810505535 1109 2 0.15747976165413746 1109 3 0.061230030806658675 1109 4 0.030514937899653205 1109 5 0.06210802099469248 1109 6 0.10139575702596204 1109 7 0.10968119259633369 1109 8 0.17172450306215126 1109 9 0.2190305669859356 1109 10-11 0.10297572579152359 1109 12-13 0.17144514953956502 1109 14-15 0.2789056204659133 1109 16-17 0.26147711379292105 1109 18-19 0.2961550104197741 1109 20-21 0.2796300567676724 1109 22-23 0.35227908842912115 1109 24-25 0.3522099105402887 1109 26-27 0.31714831886245776 1109 28-29 0.2852164301384974 1109 30-31 0.25393212823895794 1109 32-33 0.25987591880798533 1109 34-35 0.3313517541161275 1109 36-37 0.19770164955889413 1109 38-39 0.3365479284214601 1109 40-41 0.3505549526311569 1109 42-43 0.36871828389639205 1109 44-45 0.3332579418354058 1109 46-47 0.20509899516095942 1109 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-0.7083363914756227 2316 84-85 -0.7610558678666983 2316 86-87 -0.8440487365916383 2316 88-89 -0.670495109860159 2316 90-91 -0.7363890981889014 2316 92-93 -0.7809666845950147 2316 94-95 -0.7626394814548902 2316 96-97 -0.29633373046249645 2316 98-99 0.01753518878640392 2316 100-101 0.1051147033646842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13698.0 3 2716.0 4 2611.0 5 2970.0 6 3358.0 7 3986.0 8 4822.0 9 6028.0 10 7583.0 11 9885.0 12 12750.0 13 15906.0 14 18160.0 15 21537.0 16 25777.0 17 32880.0 18 29405.0 19 22479.0 20 22786.0 21 24284.0 22 26269.0 23 30265.0 24 33500.0 25 37913.0 26 44192.0 27 52130.0 28 61851.0 29 74047.0 30 91321.0 31 112881.0 32 142917.0 33 184303.0 34 247428.0 35 343607.0 36 495334.0 37 666251.0 38 499281.0 39 58760.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1492363009038 18.897851883202247 14.839703318718318 23.113208497175634 2 14.8884977217985 19.645981956770697 41.615013145074506 23.8505071763563 3 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21.402652223770044 22-23 22.174425821667693 26.550340057163112 29.262735491759003 22.01249862941019 24-25 21.942689360457088 26.697302775974673 29.436919941488842 21.923087922079397 26-27 21.751151901530402 27.05578438605183 29.252585894795267 21.940477817622504 28-29 21.760100619890395 27.22061270326116 29.27775761387117 21.74152906297727 30-31 21.851969288024094 26.920770541846345 29.248359202137152 21.978900967992406 32-33 21.714690735023314 26.882434188596378 29.255049940613453 22.14782513576686 34-35 22.12668323327396 26.96798171837659 29.139851573202456 21.765483475147 36-37 22.032260720867203 26.808868572506 29.12156570023809 22.03730500638871 38-39 21.8257092889886 27.122082482846672 29.129247768590787 21.922960459573936 40-41 22.231890472693618 26.960967891668332 28.945122818715753 21.862018816922298 42-43 21.923067304682416 26.959772756474653 28.965831549010595 22.151328389832337 44-45 22.016737545394747 27.172232650472562 28.797109107922548 22.013920696210146 46-47 22.067606747912727 27.3603710886357 28.363242265657313 22.208779897794262 48-49 22.16156673315119 27.351671409983087 28.337900820537925 22.148861036327798 50-51 22.29347676876427 27.56436950051783 28.09142858785624 22.05072514286166 52-53 22.28161753183928 27.547825411908804 28.046960694534096 22.12359636171782 54-55 22.22333848876003 27.49035351682168 28.13605382340213 22.150254171016154 56-57 22.163689541781647 27.803455874059104 28.03130417539879 22.001550408760455 58-59 22.196381537824873 27.540517666262893 28.09876266468946 22.16433813122278 60-61 22.16498808223495 27.588487267958524 28.15867476349484 22.08784988631169 62-63 22.156534460805307 27.53609337859317 28.31683993596174 21.990532224639786 64-65 22.1220693760911 27.447051295555013 28.203638926126086 22.227240402227803 66-67 22.043166584157884 27.617187022865114 28.072175666830248 22.267470726146758 68-69 22.089403811719123 27.58897699804844 28.186674216143043 22.134944974089393 70-71 22.1329108157844 27.38464377097487 28.119685778918058 22.362759634322675 72-73 22.10108111611339 27.457572950153814 28.00517960857484 22.43616632515796 74-75 22.073073222101254 27.70533251785113 27.840473401216098 22.381120858831522 76-77 22.190797749165824 27.599149498345447 27.888466084564957 22.321586667923775 78-79 22.233743176840793 27.60709469137121 27.837756531885393 22.321405599902608 80-81 22.096098323943984 27.72220594214394 27.75984573863322 22.421849995278855 82-83 22.236632427100076 27.707331613306753 27.788662081462114 22.267373878131057 84-85 22.249101935123765 27.66233824090083 27.72759587346429 22.360963950511117 86-87 22.24560673370461 27.656147181300923 27.77416032398718 22.32408576100729 88-89 22.317581824709194 27.531673130590423 27.754133093114707 22.396611951585676 90-91 22.28110668619315 27.50001077885103 27.8440285883873 22.374853946568518 92-93 22.234567086866313 27.444717928465607 27.839365574555725 22.481349410112355 94-95 22.090648968781032 27.59526434013052 27.800617107413196 22.513469583675253 96-97 22.185249034465247 27.50513464606112 27.746200405023814 22.56341591444982 98-99 22.03503407871358 27.442935327758644 27.759263506603922 22.76276708692386 100-101 22.401259814097237 27.033180335105495 28.043063478410062 22.522496372387206 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4329.0 1 2475.0 2 521.0 3 430.5 4 394.0 5 370.0 6 570.0 7 1148.0 8 2010.5 9 2569.0 10 2559.5 11 2534.5 12 2760.0 13 3102.5 14 3439.0 15 3629.5 16 3819.0 17 4106.0 18 4387.5 19 4798.5 20 5504.0 21 6505.0 22 7844.0 23 9943.5 24 12554.0 25 16001.5 26 21145.5 27 26780.0 28 31287.0 29 36318.0 30 42629.0 31 49397.0 32 57130.0 33 64786.0 34 72419.0 35 79117.5 36 87516.5 37 96014.5 38 101065.0 39 104539.0 40 108596.5 41 115067.5 42 119955.5 43 123479.0 44 127405.0 45 129196.0 46 131426.5 47 135847.5 48 138754.5 49 137703.5 50 133958.5 51 127846.5 52 120802.5 53 116612.5 54 113546.0 55 104209.0 56 91675.0 57 81179.5 58 72916.5 59 64192.0 60 52241.5 61 41249.0 62 32048.5 63 24198.5 64 17779.5 65 13095.5 66 9542.5 67 6907.0 68 5081.0 69 3680.0 70 2732.5 71 1947.0 72 1367.5 73 1025.0 74 711.5 75 470.0 76 350.0 77 226.5 78 149.0 79 106.5 80 67.5 81 44.5 82 39.5 83 33.0 84 21.0 85 16.5 86 19.0 87 13.0 88 11.0 89 10.0 90 7.5 91 5.5 92 5.0 93 5.5 94 6.0 95 5.0 96 2.0 97 3.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2934983418821072 2 0.2774909061700345 3 0.2666471593973401 4 0.2514716513477228 5 0.2500086061482323 6 0.20867040747243165 7 0.2002076950440061 8 0.21262923565928835 9 0.21262923565928835 10-11 0.21522542370936465 12-13 0.211094472557862 14-15 0.20897162266056202 16-17 0.20716433153177963 18-19 0.2078241362295891 20-21 0.19505834968501495 22-23 0.18757100072291646 24-25 0.18771443652678807 26-27 0.19961960824813246 28-29 0.2135041940629052 30-31 0.20760898252378165 32-33 0.21224195898883494 34-35 0.2064184653516472 36-37 0.1920748849644853 38-39 0.19348055584242715 40-41 0.18833121048343604 42-43 0.19992082343626288 44-45 0.19551734425740414 46-47 0.1956177493201143 48-49 0.20441036409744456 50-51 0.21307388665129037 52-53 0.22562451949005702 54-55 0.20142689937691485 56-57 0.17649775666402745 58-59 0.17857757582016592 60-61 0.183899044143803 62-63 0.192490848795713 64-65 0.18100164090559628 66-67 0.1851039048963246 68-69 0.1896221327182806 70-71 0.18414288501038475 72-73 0.18550552514716515 74-75 0.18784352875027252 76-77 0.2005806281340723 78-79 0.2063897781908729 80-81 0.19649270772373115 82-83 0.19690867155495884 84-85 0.18914879456550426 86-87 0.1895217276555705 88-89 0.1958472466063089 90-91 0.1963636155002467 92-93 0.19221832076835693 94-95 0.19349489942281434 96-97 0.1816327584426314 98-99 0.18881889221659953 100-101 0.18127416893295237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3485880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.41843134944165 #Duplication Level Percentage of deduplicated Percentage of total 1 71.03215523490012 35.81329842313695 2 15.094898505967366 15.221222080998102 3 5.759617752567698 8.711726767705796 4 2.740805702812853 5.527484966777122 5 1.5205044641265084 3.8330724970540957 6 0.9451774975075372 2.8592620062672496 7 0.5902970889454646 2.0833297278340512 8 0.4239216713840713 1.709877254897469 9 0.30740844561361313 1.3949146450267669 >10 1.4287175041651907 12.836894588445627 >50 0.10071477791536991 3.487873058460637 >100 0.05344717737936444 4.831366837348975 >500 0.0018787276017168911 0.6426203928342927 >1k 2.846556956854218E-4 0.3065564483884143 >5k 1.1386227827416868E-4 0.4136502788132433 >10k+ 5.693113913708434E-5 0.32685002601113045 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11387 0.3266606997372256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8630 0.2475701974824148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5781 0.16584047643636612 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4803 0.13778443319907743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4343580387161923E-5 0.0 12-13 0.0 0.0 0.0 2.8687160774323845E-5 0.0 14-15 0.0 0.0 0.0 2.8687160774323845E-5 0.0 16-17 0.0 0.0 0.0 5.737432154864769E-5 0.0 18-19 0.0 0.0 0.0 5.737432154864769E-5 0.0 20-21 0.0 0.0 0.0 7.171790193580961E-5 0.0 22-23 0.0 0.0 0.0 1.2909222348445732E-4 0.0 24-25 0.0 0.0 0.0 2.581844469689146E-4 0.0 26-27 0.0 0.0 0.0 3.299023489047242E-4 0.0 28-29 0.0 0.0 0.0 7.745533409067438E-4 0.0 30-31 0.0 0.0 0.0 0.0016925424856851067 0.0 32-33 0.0 0.0 0.0 0.0030838697832398132 0.0 34-35 0.0 0.0 0.0 0.004575602143504652 0.0 36-37 2.8687160774323845E-5 0.0 0.0 0.00694229290738637 0.0 38-39 2.8687160774323845E-5 0.0 0.0 0.008907363420427554 0.0 40-41 2.8687160774323845E-5 0.0 0.0 0.011532238631278186 0.0 42-43 2.8687160774323845E-5 0.0 0.0 0.0164233995433004 0.0 44-45 2.8687160774323845E-5 0.0 0.0 0.023179225905653668 0.0 46-47 2.8687160774323845E-5 0.0 0.0 0.038082205927914906 0.0 48-49 2.8687160774323845E-5 0.0 0.0 0.06741482781966103 0.0 50-51 2.8687160774323845E-5 0.0 0.0 0.08981950038440795 0.0 52-53 2.8687160774323845E-5 0.0 0.0 0.1129270083881258 0.0 54-55 2.8687160774323845E-5 0.0 0.0 0.14534350006311175 0.0 56-57 2.8687160774323845E-5 0.0 0.0 0.16892434621960595 0.0 58-59 2.8687160774323845E-5 0.0 0.0 0.19457066795185146 0.0 60-61 2.8687160774323845E-5 0.0 0.0 0.23837596245424397 0.0 62-63 2.8687160774323845E-5 0.0 0.0 0.26964496769825697 0.0 64-65 2.8687160774323845E-5 0.0 0.0 0.30546088792500026 0.0 66-67 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.3711257989374276 0.0 68-69 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.4438764386611128 0.0 70-71 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.5093692267088942 0.0 72-73 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.5844865571964611 0.0 74-75 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.6596469184251896 0.0 76-77 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.7271478077271736 0.0 78-79 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.811860993493752 0.0 80-81 2.8687160774323845E-5 2.8687160774323845E-5 0.0 0.9346563851882452 0.0 82-83 2.8687160774323845E-5 2.8687160774323845E-5 0.0 1.043509816746417 0.0 84-85 2.8687160774323845E-5 2.8687160774323845E-5 0.0 1.1674096641307217 0.0 86-87 2.8687160774323845E-5 2.8687160774323845E-5 0.0 1.3738568166431433 0.0 88-89 2.8687160774323845E-5 2.8687160774323845E-5 0.0 1.6416371188910692 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6255 0.0 48.341877 1 GTATCAA 11225 0.0 42.31291 1 ATCAACG 13965 0.0 33.656418 3 TCAACGC 14240 0.0 32.937336 4 CAACGCA 14495 0.0 32.358826 5 TATCAAC 14650 0.0 32.28061 2 AACGCAG 14965 0.0 31.426512 6 ACGCAGA 17505 0.0 26.595484 7 CGCAGAG 17445 0.0 26.57803 8 GTACATG 12675 0.0 23.78127 1 GCAGAGT 19310 0.0 23.419287 9 TACATGG 12500 0.0 23.194025 2 ACATGGG 12555 0.0 21.840956 3 GAGTACT 10915 0.0 21.606287 12-13 TGGTATC 3325 0.0 21.58448 2 AGTACTT 11525 0.0 20.689377 12-13 AGAGTAC 17850 0.0 20.477057 10-11 CAGAGTA 18465 0.0 19.807905 10-11 GTACTTT 11855 0.0 19.671453 14-15 GTGGTAT 4100 0.0 18.901554 1 >>END_MODULE