FastQCFastQC Report
Thu 26 May 2016
SRR937589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485880
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142590.40905022548108366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115820.3322546960882188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76640.21985840017441793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.048.8795361
GTATCAA131450.040.670971
ATCAACG163600.032.2003173
TCAACGC166600.031.648994
TATCAAC169550.031.2103982
CAACGCA170450.030.9341265
AACGCAG175050.030.1996086
ACGCAGA200400.026.2632057
CGCAGAG200550.026.1725048
TACATGG122500.023.8867232
GTACATG127950.023.8815941
GCAGAGT219100.023.5663789
GAGTACT130850.022.28950112-13
ACATGGG124100.022.1242223
AGTACTT139450.021.2725512-13
AGAGTAC202100.021.0712210-11
GTACTTT139950.020.61806514-15
CAGAGTA210400.020.30771610-11
TGGTATC38350.020.0660342
AGGACCG11800.019.3228475
Produced by FastQC (version 0.11.5)