##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937589_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3485880 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29521526845445 33.0 31.0 34.0 31.0 34.0 2 32.506786521624385 34.0 31.0 34.0 31.0 34.0 3 32.57762688331211 34.0 31.0 34.0 31.0 34.0 4 36.15976826511527 37.0 35.0 37.0 35.0 37.0 5 36.08264369398832 37.0 35.0 37.0 35.0 37.0 6 36.124375193638336 37.0 35.0 37.0 35.0 37.0 7 36.08212703822277 37.0 35.0 37.0 35.0 37.0 8 36.04756073071936 37.0 35.0 37.0 35.0 37.0 9 37.756778776090975 39.0 37.0 39.0 35.0 39.0 10-11 37.67796338370799 39.0 37.0 39.0 35.0 39.0 12-13 37.57872588844137 39.0 37.0 39.0 35.0 39.0 14-15 38.837916107267034 40.0 38.0 41.0 35.0 41.0 16-17 38.79577108219445 40.0 38.0 41.0 35.0 41.0 18-19 38.7605772430491 40.0 38.0 41.0 35.0 41.0 20-21 38.652962523093166 40.0 38.0 41.0 34.0 41.0 22-23 38.604030115781384 40.0 38.0 41.0 34.0 41.0 24-25 38.62957832742378 40.0 38.0 41.0 34.0 41.0 26-27 38.46145377924656 40.0 38.0 41.0 34.0 41.0 28-29 38.43671167682192 40.0 38.0 41.0 34.0 41.0 30-31 38.460753382216254 40.0 38.0 41.0 34.0 41.0 32-33 38.34647893788656 40.0 38.0 41.0 34.0 41.0 34-35 38.20987211263727 40.0 38.0 41.0 34.0 41.0 36-37 38.04836497527167 40.0 38.0 41.0 33.5 41.0 38-39 37.912898321227345 40.0 37.5 41.0 33.0 41.0 40-41 37.750437909509216 40.0 37.0 41.0 33.0 41.0 42-43 37.53969069503253 40.0 37.0 41.0 33.0 41.0 44-45 37.25739741471307 40.0 37.0 41.0 32.0 41.0 46-47 36.950027539674345 40.0 37.0 41.0 31.0 41.0 48-49 36.636962976350304 40.0 36.0 41.0 31.0 41.0 50-51 36.40568995490378 39.0 36.0 41.0 30.0 41.0 52-53 36.14688772992759 39.0 35.5 40.5 30.0 41.0 54-55 35.92808917690799 39.0 35.0 40.0 29.0 41.0 56-57 35.658671268087254 39.0 35.0 40.0 28.0 41.0 58-59 35.407193592435775 38.0 35.0 40.0 28.0 41.0 60-61 35.06502877322225 38.0 34.0 40.0 27.5 41.0 62-63 34.664830831812914 38.0 34.0 40.0 26.5 41.0 64-65 34.26948245493247 37.0 34.0 40.0 26.0 41.0 66-67 33.85127500086061 36.5 33.0 39.0 26.0 40.5 68-69 33.381836580719934 36.0 33.0 39.0 25.0 40.0 70-71 32.958439332392395 35.5 32.0 38.5 24.0 40.0 72-73 32.48015264438248 35.0 32.0 38.0 22.5 40.0 74-75 31.997340843632024 35.0 31.0 37.0 21.5 39.0 76-77 30.721736261718704 34.0 29.5 36.0 20.5 38.5 78-79 31.326488433336777 34.0 31.0 36.0 21.0 38.5 80-81 31.157748402125144 34.0 31.0 36.0 20.0 37.5 82-83 30.766579601133717 34.0 31.0 35.0 19.0 37.0 84-85 30.360681951185928 34.0 30.5 35.0 18.0 36.5 86-87 30.015976740450043 34.0 30.0 35.0 14.0 36.0 88-89 29.60721826339404 34.0 30.0 35.0 7.0 36.0 90-91 29.107971014492755 34.0 29.0 35.0 3.5 35.0 92-93 28.644122861372164 34.0 29.0 35.0 2.0 35.0 94-95 28.1518227821956 33.0 29.0 35.0 2.0 35.0 96-97 27.63189122976121 33.0 27.0 35.0 2.0 35.0 98-99 26.813365204768957 33.0 26.0 34.5 2.0 35.0 100-101 24.306616837068404 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2156107204355564 1101 2 0.2007219977364727 1101 3 0.23194940872320302 1101 4 0.11165384180205962 1101 5 0.11896170284857988 1101 6 0.12266916794678906 1101 7 0.10718734991012013 1101 8 0.14200140938675077 1101 9 0.2329443161224276 1101 10-11 0.22539351263829133 1101 12-13 0.20641664482742073 1101 14-15 0.304603552387114 1101 16-17 0.30403924799316684 1101 18-19 0.3376983268580105 1101 20-21 0.2490369638184049 1101 22-23 0.2505335035367082 1101 24-25 0.2496385365396705 1101 26-27 0.16190694455003296 1101 28-29 0.2980522105703898 1101 30-31 0.4106374581873311 1101 32-33 0.40528054908687494 1101 34-35 0.4271913573850341 1101 36-37 0.3790544292553406 1101 38-39 0.4721141195606364 1101 40-41 0.3642412723378783 1101 42-43 0.4134634063214051 1101 44-45 0.5646731846993944 1101 46-47 0.6286497252851362 1101 48-49 0.5340652017652374 1101 50-51 0.5176981423886531 1101 52-53 0.5436255059836554 1101 54-55 0.6318839151524642 1101 56-57 0.6314186112861861 1101 58-59 0.6046137578105899 1101 60-61 0.6791947585069806 1101 62-63 0.6960099105323181 1101 64-65 0.8109909381723952 1101 66-67 0.7307582455690422 1101 68-69 0.7905543589578414 1101 70-71 0.7352386091839236 1101 72-73 0.7595862693614492 1101 74-75 0.7924975509674788 1101 76-77 0.6875876400588474 1101 78-79 0.7529110415317462 1101 80-81 0.7945496367372442 1101 82-83 0.7771183595501086 1101 84-85 0.8305043050199146 1101 86-87 0.8242129133404994 1101 88-89 0.8832872994203669 1101 90-91 0.8952908586735298 1101 92-93 1.1381202606634346 1101 94-95 1.0140422714365123 1101 96-97 1.2061243693693982 1101 98-99 1.3705415302469994 1101 100-101 1.8051486146774884 1102 1 0.17077423597850583 1102 2 0.14312791182961604 1102 3 0.1709682131101502 1102 4 0.14876931969642726 1102 5 0.017402006070668108 1102 6 0.09856291511424331 1102 7 0.06353122186582993 1102 8 0.15491837245750162 1102 9 0.18783133001262087 1102 10-11 0.19701697965342646 1102 12-13 0.1712041948891425 1102 14-15 0.24542530392628237 1102 16-17 0.30557393878613226 1102 18-19 0.2552332371463777 1102 20-21 0.23247117405291107 1102 22-23 0.20215275123613452 1102 24-25 0.20032528419216789 1102 26-27 0.24086465699855353 1102 28-29 0.21575060512644484 1102 30-31 0.1973055578291465 1102 32-33 0.3083415574350923 1102 34-35 0.2898584596306577 1102 36-37 0.28317332297578446 1102 38-39 0.38354502485405817 1102 40-41 0.4477668773860515 1102 42-43 0.41498402325132844 1102 44-45 0.4782504962674281 1102 46-47 0.443842844274954 1102 48-49 0.509288876675491 1102 50-51 0.4776323421033126 1102 52-53 0.5171567633226815 1102 54-55 0.48274432574533677 1102 56-57 0.6057803687051049 1102 58-59 0.6634661039377718 1102 60-61 0.5916049345638967 1102 62-63 0.5579834840823281 1102 64-65 0.5688831056553454 1102 66-67 0.5241788141076213 1102 68-69 0.5048759788566386 1102 70-71 0.5486747698568237 1102 72-73 0.5485909145892194 1102 74-75 0.7035917497495667 1102 76-77 0.6241365525614988 1102 78-79 0.5542434667455112 1102 80-81 0.6488402315868349 1102 82-83 0.7011338572900421 1102 84-85 0.6765738446880221 1102 86-87 0.7126030567460546 1102 88-89 0.7834891100868191 1102 90-91 0.8266404028225232 1102 92-93 0.9409536136148802 1102 94-95 1.0701828400433264 1102 96-97 1.0886634819142458 1102 98-99 1.3254275490155614 1102 100-101 1.7574995610950204 1103 1 0.213819154051329 1103 2 0.20071276625713352 1103 3 0.1971447534200479 1103 4 0.09375664091847113 1103 5 0.1342609016880516 1103 6 0.14664881473280644 1103 7 0.1129141640606548 1103 8 0.14659692772332278 1103 9 0.22311762615726138 1103 10-11 0.211999954892093 1103 12-13 0.16627509547746655 1103 14-15 0.2247580697675744 1103 16-17 0.1869672754238465 1103 18-19 0.2709222012219783 1103 20-21 0.34336421976116327 1103 22-23 0.3585750012343354 1103 24-25 0.23865175733687494 1103 26-27 0.17168418016156295 1103 28-29 0.20460150557110524 1103 30-31 0.24485679374061675 1103 32-33 0.33019686133067694 1103 34-35 0.2639516576279064 1103 36-37 0.2091636684275784 1103 38-39 0.29156067039421174 1103 40-41 0.32341978057478116 1103 42-43 0.438287293873195 1103 44-45 0.4802547129488275 1103 46-47 0.5115358813630806 1103 48-49 0.43711082065900086 1103 50-51 0.4276098938420603 1103 52-53 0.36633659364974136 1103 54-55 0.5198529896966662 1103 56-57 0.48830991061691265 1103 58-59 0.4625678861501328 1103 60-61 0.4765612592268127 1103 62-63 0.29610706086256045 1103 64-65 0.422736017464203 1103 66-67 0.5309987036262598 1103 68-69 0.5685442043305642 1103 70-71 0.5059683356464006 1103 72-73 0.5078314090974771 1103 74-75 0.6304355242181678 1103 76-77 0.4717897215376432 1103 78-79 0.6496183706669498 1103 80-81 0.7005633960374134 1103 82-83 0.7462315880402137 1103 84-85 0.6774870399555688 1103 86-87 0.7357218206190801 1103 88-89 0.9230772832652754 1103 90-91 0.9138728375070677 1103 92-93 1.0709160801220747 1103 94-95 1.0896967723321893 1103 96-97 1.0740623426200884 1103 98-99 1.2901638088838183 1103 100-101 1.9968007197844742 1104 1 0.18799957662997713 1104 2 0.1840520812294315 1104 3 0.14305186339674947 1104 4 0.1254815449707536 1104 5 0.12596135904193773 1104 6 0.10278029190040883 1104 7 0.12067580503600084 1104 8 0.11310591690064342 1104 9 0.141332051195441 1104 10-11 0.14848787829739507 1104 12-13 0.21132463905240684 1104 14-15 0.24523448316198682 1104 16-17 0.2575748390391368 1104 18-19 0.2824362617888596 1104 20-21 0.18915262578441627 1104 22-23 0.23780912852806324 1104 24-25 0.15128557328815617 1104 26-27 0.13211679012526645 1104 28-29 0.20095359747286068 1104 30-31 0.1922708309010872 1104 32-33 0.24009483909086526 1104 34-35 0.2749864068634835 1104 36-37 0.31020369339442766 1104 38-39 0.3018851278669885 1104 40-41 0.3412050508816975 1104 42-43 0.38405426259405573 1104 44-45 0.3869058867990347 1104 46-47 0.3633963568476446 1104 48-49 0.3899973515684678 1104 50-51 0.38363685951636484 1104 52-53 0.483581175808105 1104 54-55 0.48803223080872726 1104 56-57 0.4379270857228903 1104 58-59 0.40039253867634983 1104 60-61 0.363682976291571 1104 62-63 0.29508401483366953 1104 64-65 0.5344710642956159 1104 66-67 0.5477102511617105 1104 68-69 0.4595831890123705 1104 70-71 0.5690932404417381 1104 72-73 0.4507571736140932 1104 74-75 0.6682201257704286 1104 76-77 0.6105596587861655 1104 78-79 0.6466028072597183 1104 80-81 0.6262811369841437 1104 82-83 0.5287535733498991 1104 84-85 0.5836579736485454 1104 86-87 0.7149994541192299 1104 88-89 0.7089210844668052 1104 90-91 0.7148935428946253 1104 92-93 0.758373177652512 1104 94-95 0.7768423044937158 1104 96-97 1.1097916010083182 1104 98-99 1.2424191468464159 1104 100-101 1.5903557824430727 1105 1 0.16296632228830532 1105 2 0.11036312725331499 1105 3 0.13713756826319212 1105 4 0.117148498513302 1105 5 0.03507954601119678 1105 6 0.018729381201303852 1105 7 0.07047190606716924 1105 8 0.11358368153899079 1105 9 0.15103636435100753 1105 10-11 0.14425157444962622 1105 12-13 0.13730616548638608 1105 14-15 0.10130652901499104 1105 16-17 0.1825320635196661 1105 18-19 0.2067359202359924 1105 20-21 0.2036359809358217 1105 22-23 0.2331452245536454 1105 24-25 0.13820511871549712 1105 26-27 0.23752188832830967 1105 28-29 0.2034139845661116 1105 30-31 0.08801455428947946 1105 32-33 0.13700676757596142 1105 34-35 0.21740064880871302 1105 36-37 0.17776348995755598 1105 38-39 0.18811527179184395 1105 40-41 0.2071252354995039 1105 42-43 0.2992817427600798 1105 44-45 0.2756170418667594 1105 46-47 0.3580391436560717 1105 48-49 0.35861483623456536 1105 50-51 0.2199814591632432 1105 52-53 0.24473824391802879 1105 54-55 0.3415184395222539 1105 56-57 0.3529128148109564 1105 58-59 0.36823824969589225 1105 60-61 0.39085554934490574 1105 62-63 0.3684283300857558 1105 64-65 0.357313889498883 1105 66-67 0.34766200949375303 1105 68-69 0.3571732300464774 1105 70-71 0.24877182160636835 1105 72-73 0.28495989592661886 1105 74-75 0.3453960638002762 1105 76-77 0.28130252154691604 1105 78-79 0.3777868510062774 1105 80-81 0.44054265179422814 1105 82-83 0.5079079314907098 1105 84-85 0.5107305588281967 1105 86-87 0.6921833626464533 1105 88-89 0.7358427574276831 1105 90-91 0.8156263634489598 1105 92-93 0.8078353861749896 1105 94-95 0.9455046968648624 1105 96-97 1.0215157839625562 1105 98-99 1.0469854199814073 1105 100-101 1.3585617785521045 1106 1 -0.4361271274915879 1106 2 -0.2711673797406604 1106 3 -0.2970332814302594 1106 4 -0.19872544282411297 1106 5 -0.19492265052070934 1106 6 -0.20103110572022587 1106 7 -0.19201871818314942 1106 8 -0.20396802553070614 1106 9 -0.5451103923228544 1106 10-11 -0.5436698281726464 1106 12-13 -0.608790688411851 1106 14-15 -0.518106106205849 1106 16-17 -0.40567741881748276 1106 18-19 -0.4902454173405957 1106 20-21 -0.3779064703330519 1106 22-23 -0.512250662364444 1106 24-25 -0.48772752164745015 1106 26-27 -0.4273559207272726 1106 28-29 -0.5707954410863465 1106 30-31 -0.6198894684005936 1106 32-33 -0.6629281462481131 1106 34-35 -0.9334760407241589 1106 36-37 -0.7476065548132311 1106 38-39 -0.730613047577819 1106 40-41 -0.8337499964269313 1106 42-43 -1.0212474291069071 1106 44-45 -1.0202962953332744 1106 46-47 -0.9582973852769072 1106 48-49 -1.2015751992034893 1106 50-51 -1.1192167155895945 1106 52-53 -1.1411124465724072 1106 54-55 -1.2356210787185873 1106 56-57 -1.4620440491488154 1106 58-59 -1.4592866738556936 1106 60-61 -1.4801169271992762 1106 62-63 -1.6149865887184802 1106 64-65 -2.138265716562735 1106 66-67 -2.1807777645367636 1106 68-69 -2.000716469635993 1106 70-71 -1.825845480789269 1106 72-73 -2.0431263073224244 1106 74-75 -2.0357862372136744 1106 76-77 -1.822562564831447 1106 78-79 -1.732855346092478 1106 80-81 -1.8368854016634906 1106 82-83 -1.9852686484180104 1106 84-85 -1.786428829422487 1106 86-87 -1.6971261064305274 1106 88-89 -1.9246113679527852 1106 90-91 -2.1856429688154613 1106 92-93 -2.143271534317641 1106 94-95 -1.9309274773676925 1106 96-97 -2.1827654794178173 1106 98-99 -2.1730167360614345 1106 100-101 -1.567130913474891 1107 1 0.16587527937794277 1107 2 0.2230461801641468 1107 3 0.16002008290886494 1107 4 0.14212709569937232 1107 5 0.13535232979906198 1107 6 0.12473024019639212 1107 7 0.07823985440447245 1107 8 0.05646098840681901 1107 9 0.1780106358145943 1107 10-11 0.16005674782923052 1107 12-13 0.19827896301433157 1107 14-15 0.2393686111076292 1107 16-17 0.23270400666456936 1107 18-19 0.30071132837876036 1107 20-21 0.20568474187864183 1107 22-23 0.20773208538779642 1107 24-25 0.26369382831077814 1107 26-27 0.2050792099162777 1107 28-29 0.25671299652958623 1107 30-31 0.1987809623928527 1107 32-33 0.2465269602060971 1107 34-35 0.31136788977220675 1107 36-37 0.29485297629963725 1107 38-39 0.2940716917232251 1107 40-41 0.26271706293950103 1107 42-43 0.3813309072576416 1107 44-45 0.3810899167426882 1107 46-47 0.15147004115220852 1107 48-49 0.13442288253958878 1107 50-51 0.1613668145460494 1107 52-53 0.21898976997949404 1107 54-55 0.25372294376965954 1107 56-57 0.32329742169579134 1107 58-59 0.2644028942650536 1107 60-61 0.28955802476889403 1107 62-63 0.43065118646542544 1107 64-65 0.44686749069647647 1107 66-67 0.5229940533736013 1107 68-69 0.5899558552853605 1107 70-71 0.43622732248986296 1107 72-73 0.39417670186771403 1107 74-75 0.514126144290227 1107 76-77 0.4017355069584134 1107 78-79 0.5496781102988457 1107 80-81 0.6362484450146972 1107 82-83 0.6734588173414089 1107 84-85 0.6635132609346535 1107 86-87 0.9033493860276494 1107 88-89 0.9775273678676442 1107 90-91 1.0404527148048537 1107 92-93 0.9104396594353581 1107 94-95 1.0468353705802862 1107 96-97 1.0463372830531164 1107 98-99 1.0954268819641477 1107 100-101 1.6092182756393036 1108 1 0.09955744936945621 1108 2 0.0639060435680534 1108 3 0.0988414320915183 1108 4 0.07937488821739436 1108 5 0.014669064953359623 1108 6 0.08168682993888865 1108 7 0.04864845777814253 1108 8 0.07265535109267063 1108 9 0.10966424480471915 1108 10-11 0.09189591327545088 1108 12-13 0.04470646802648304 1108 14-15 0.09028027865112165 1108 16-17 0.15082882004169562 1108 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60-61 -0.24991284654453239 2316 62-63 -0.26173334949512395 2316 64-65 -0.35603400667447715 2316 66-67 -0.2317395287634767 2316 68-69 -0.23340088015483929 2316 70-71 -0.20823975081399482 2316 72-73 -0.09220191874979378 2316 74-75 -0.14207325348645483 2316 76-77 -0.12583275487227397 2316 78-79 -0.06230915840919593 2316 80-81 -0.04547209880568559 2316 82-83 -0.11175379159551468 2316 84-85 0.020194687530729283 2316 86-87 0.10113670431449506 2316 88-89 0.32629636928677996 2316 90-91 0.1965221956374137 2316 92-93 0.27608450232966675 2316 94-95 0.27497464154438944 2316 96-97 0.47619066168964963 2316 98-99 0.39876787954433723 2316 100-101 0.9128844920030375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 8.0 4 25.0 5 60.0 6 106.0 7 302.0 8 772.0 9 1445.0 10 2622.0 11 4368.0 12 6846.0 13 9886.0 14 13125.0 15 17646.0 16 23367.0 17 32224.0 18 29691.0 19 19330.0 20 16612.0 21 16842.0 22 17071.0 23 18599.0 24 21407.0 25 25352.0 26 30699.0 27 38159.0 28 47448.0 29 59989.0 30 75146.0 31 95574.0 32 123732.0 33 163556.0 34 227213.0 35 328935.0 36 517352.0 37 808342.0 38 637740.0 39 54277.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.338058298065654 19.469938795382234 15.442062527030103 22.74994037952201 2 15.342209140876909 20.170344360677937 40.75386989798845 23.7335766004567 3 19.29931609808714 23.986912917254756 30.146390581431376 26.567380403226732 4 12.959539628443896 16.90838468335112 37.13464032037821 32.997435367826775 5 13.63232880799642 37.46410906712264 34.514798195985726 14.388763928895212 6 32.3632483045888 36.47520855565883 17.089142483390134 14.072400656362237 7 28.672989316901326 32.91120176253916 20.282252974858572 18.13355594570094 8 26.215245504721906 35.90731752097032 19.290853385658714 18.586583588649063 9 25.98173201601891 17.112809391028954 20.285408562543747 36.62005003040839 10-11 24.81158272803424 27.146731958644587 27.130667148610964 20.911018164710203 12-13 25.60152841434614 24.12055068359218 28.031410584490253 22.24651031757143 14-15 22.528572412131226 25.593924633091213 26.37731361951645 25.50018933526111 16-17 21.838947410696868 29.001901958759333 27.040575120199208 22.11857551034459 18-19 21.703271483814703 27.48590026047942 29.58734953584174 21.22347871986414 20-21 22.343951774442502 26.450258680079674 30.020343561341008 21.185445984136813 22-23 22.098682379806245 26.362114618326192 29.82909378827998 21.71010921358758 24-25 21.956521739130437 26.454195210391635 30.007200477354356 21.58208257312357 26-27 21.729536965331604 26.826485606556012 29.847435647155972 21.596541780956414 28-29 21.658608542410185 26.957262130182013 29.891364139766846 21.492765187640956 30-31 21.84167269097043 26.733493407690457 29.769383914535208 21.655449986803905 32-33 21.72371108586641 26.619246789906708 29.81859673884356 21.838445385383316 34-35 22.14536931850781 26.669492352002937 29.71278414632747 21.472354183161784 36-37 22.059162292099906 26.58903705216691 29.635192831400147 21.716607824333042 38-39 21.812368756239458 26.87672840143665 29.71946825478789 21.591434587536 40-41 22.22705282689344 26.719998072220584 29.464042897681956 21.588906203204015 42-43 21.980547591259832 26.682194353364146 29.446677316546026 21.89058073883 44-45 22.030807141955545 26.919472270990397 29.321778144973436 21.72794244208062 46-47 22.095138099991967 27.194912045165065 28.814617829644163 21.8953320251988 48-49 22.242676167854313 27.2214620124617 28.67466751580662 21.861194303877358 50-51 22.3026897081942 27.446598850218596 28.460431799143976 21.790279642443227 52-53 22.377606228556346 27.43004635845181 28.27892813292483 21.91341928006701 54-55 22.288016225458136 27.429573020299035 28.3983958139695 21.88401494027333 56-57 22.22298243198274 27.650679311967135 28.363383134244437 21.762955121805685 58-59 22.276641766211114 27.41230334951289 28.394594765166904 21.91646011910909 60-61 22.24497114071626 27.453096492133984 28.435430938529148 21.86650142862061 62-63 22.180267249589775 27.415100347688387 28.620821715033216 21.783810687688618 64-65 22.190293412280397 27.2845881097456 28.529237954261188 21.995880523712806 66-67 22.13828358979655 27.39669753405166 28.385916899032672 22.07910197711912 68-69 22.21147888051224 27.36294708940067 28.475004876817334 21.950569153269765 70-71 22.241471307101794 27.250952413737707 28.341380081930534 22.16619619722997 72-73 22.29591953825146 27.229006735745347 28.235122838422438 22.239950887580754 74-75 22.160057144824265 27.554993287204375 28.006543541372626 22.278406026598734 76-77 22.28585034481967 27.437877953343204 28.168869840614136 22.107401861222993 78-79 22.341259595855277 27.47073909601019 28.07131915040105 22.116682157733486 80-81 22.207247524298023 27.5833505456298 28.01867821038016 22.190723719692016 82-83 22.298254539790122 27.51062395730881 28.061275351085087 22.12984615181598 84-85 22.251360492502588 27.50346398460069 27.973317995220704 22.271857527676016 86-87 22.241456963521404 27.45946504182588 28.027729009604464 22.27134898504825 88-89 22.351285758545906 27.393541946366483 28.01136298438271 22.2438093107049 90-91 22.277975158057636 27.37175394234972 28.109463834126164 22.24080706546648 92-93 22.132884807042085 27.245662814642092 28.181391513994264 22.440060864321563 94-95 22.047044074953813 27.39235142919435 28.115454347252346 22.445150148599495 96-97 22.127597622408114 27.264134164113507 28.09910266561098 22.509165547867397 98-99 21.956439543895524 27.151644870086876 28.045927155015622 22.845988431001977 100-101 22.004447371550622 27.213547139599292 28.184991261478682 22.597014227371403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 183.0 2 190.5 3 281.0 4 262.5 5 282.0 6 565.0 7 1300.0 8 2193.0 9 2746.5 10 2823.5 11 2826.5 12 3181.0 13 3500.0 14 3813.5 15 4225.0 16 4549.5 17 4899.0 18 5268.5 19 5728.0 20 6375.5 21 7254.5 22 8583.0 23 10583.5 24 13047.5 25 16457.5 26 21372.5 27 26894.0 28 31425.0 29 36573.0 30 43206.5 31 49926.0 32 57173.0 33 64753.0 34 72183.5 35 78769.0 36 87314.0 37 95694.0 38 101198.0 39 105584.5 40 109406.0 41 115151.0 42 119287.5 43 122900.5 44 127471.0 45 129630.0 46 131933.0 47 136602.5 48 139763.0 49 138186.5 50 134246.0 51 128064.0 52 120766.5 53 117212.0 54 115088.5 55 105075.0 56 90938.0 57 80199.5 58 72152.0 59 62576.0 60 50406.5 61 39890.0 62 31149.5 63 23555.5 64 17018.5 65 11996.0 66 8671.0 67 6503.0 68 4941.5 69 3678.0 70 2602.5 71 1624.0 72 1140.5 73 912.5 74 624.0 75 430.5 76 299.0 77 168.5 78 102.5 79 69.0 80 52.0 81 35.0 82 17.0 83 10.0 84 7.5 85 4.5 86 4.0 87 4.5 88 2.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03841210827681963 2 0.0 3 0.0 4 0.0 5 0.00917989144778363 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005048940296280997 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.0121518813040033E-4 22-23 0.0014343580387161923 24-25 0.0 26-27 0.0011331428505857919 28-29 0.0017355732268465925 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.155587685175623E-4 38-39 0.0 40-41 1.1474864309729537E-4 42-43 0.02029616624783412 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.4384086658175267E-4 84-85 2.8687160774323845E-4 86-87 0.0 88-89 0.0 90-91 0.010427782941466717 92-93 0.0026965931127864414 94-95 0.0 96-97 0.0 98-99 3.4424592929188614E-4 100-101 0.01292356592883289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3485880.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.26536939388182 #Duplication Level Percentage of deduplicated Percentage of total 1 69.0692197831807 33.33651406582426 2 15.944496603480243 15.39134036732936 3 6.080786832928271 8.804742680902072 4 3.05444687066272 5.896960260260902 5 1.6712453254274489 4.033163648977704 6 1.0279014412418572 2.976722565722505 7 0.6454654544884121 2.1807540014311115 8 0.46194686098876514 1.783682870877354 9 0.33386116471255906 1.4502539197010955 >10 1.5418726875018691 13.354767834149753 >50 0.1083821357046023 3.615502587498355 >100 0.05721965448393327 5.042065834057974 >500 0.002559867239326893 0.8459928246192662 >1k 4.167225714839681E-4 0.324829291886189 >5k 5.953179592628113E-5 0.22021156063827965 >10k+ 1.1906359185256226E-4 0.742495686123928 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14259 0.40905022548108366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11582 0.3322546960882188 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7664 0.21985840017441793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.4343580387161923E-5 0.0 0.0 0.0 0.0 16-17 2.8687160774323845E-5 0.0 0.0 2.8687160774323845E-5 0.0 18-19 2.8687160774323845E-5 0.0 0.0 2.8687160774323845E-5 0.0 20-21 2.8687160774323845E-5 0.0 0.0 5.737432154864769E-5 0.0 22-23 2.8687160774323845E-5 0.0 0.0 1.2909222348445732E-4 0.0 24-25 5.737432154864769E-5 0.0 0.0 2.581844469689146E-4 0.0 26-27 5.737432154864769E-5 0.0 0.0 3.299023489047242E-4 0.0 28-29 5.737432154864769E-5 0.0 0.0 7.888969212939056E-4 0.0 30-31 5.737432154864769E-5 0.0 0.0 0.0017212296464594306 0.0 32-33 5.737432154864769E-5 0.0 0.0 0.0031699312655627844 0.0 34-35 5.737432154864769E-5 0.0 0.0 0.004647320045440462 0.0 36-37 5.737432154864769E-5 0.0 0.0 0.006956636487773532 0.0 38-39 5.737432154864769E-5 0.0 0.0 0.008907363420427554 0.0 40-41 5.737432154864769E-5 0.0 0.0 0.01156092579205251 0.0 42-43 5.737432154864769E-5 0.0 0.0 0.016466430284461885 0.0 44-45 5.737432154864769E-5 0.0 0.0 0.023150538744879344 0.0 46-47 5.737432154864769E-5 0.0 0.0 0.03802483160636626 0.0 48-49 5.737432154864769E-5 0.0 0.0 0.06760129436469414 0.0 50-51 5.737432154864769E-5 0.0 0.0 0.09009202841176403 0.0 52-53 8.606148232297154E-5 0.0 0.0 0.11318519283509473 0.0 54-55 8.606148232297154E-5 0.0 0.0 0.14534350006311175 0.0 56-57 8.606148232297154E-5 0.0 0.0 0.16896737696076744 0.0 58-59 8.606148232297154E-5 0.0 0.0 0.194441575728367 0.0 60-61 8.606148232297154E-5 0.0 0.0 0.23823252665037237 0.0 62-63 8.606148232297154E-5 0.0 0.0 0.26947284473361105 0.0 64-65 8.606148232297154E-5 0.0 0.0 0.3053891700230645 0.0 66-67 8.606148232297154E-5 0.0 0.0 0.37096801955316877 0.0 68-69 8.606148232297154E-5 0.0 0.0 0.44371865927685405 0.0 70-71 8.606148232297154E-5 0.0 0.0 0.5094266010304428 0.0 72-73 8.606148232297154E-5 0.0 0.0 0.5848021159649788 0.0 74-75 8.606148232297154E-5 0.0 0.0 0.6602493488014505 0.0 76-77 8.606148232297154E-5 0.0 0.0 0.7277502381034344 0.0 78-79 8.606148232297154E-5 0.0 0.0 0.8130084799247249 0.0 80-81 8.606148232297154E-5 0.0 0.0 0.9365640813797377 0.0 82-83 8.606148232297154E-5 0.0 0.0 1.0462494406003648 0.0 84-85 8.606148232297154E-5 0.0 0.0 1.1708808105844148 0.0 86-87 8.606148232297154E-5 0.0 0.0 1.3785901981709068 0.0 88-89 8.606148232297154E-5 0.0 0.0 1.6503436721860765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7350 0.0 48.879536 1 GTATCAA 13145 0.0 40.67097 1 ATCAACG 16360 0.0 32.200317 3 TCAACGC 16660 0.0 31.64899 4 TATCAAC 16955 0.0 31.210398 2 CAACGCA 17045 0.0 30.934126 5 AACGCAG 17505 0.0 30.199608 6 ACGCAGA 20040 0.0 26.263205 7 CGCAGAG 20055 0.0 26.172504 8 TACATGG 12250 0.0 23.886723 2 GTACATG 12795 0.0 23.881594 1 GCAGAGT 21910 0.0 23.566378 9 GAGTACT 13085 0.0 22.289501 12-13 ACATGGG 12410 0.0 22.124222 3 AGTACTT 13945 0.0 21.27255 12-13 AGAGTAC 20210 0.0 21.07122 10-11 GTACTTT 13995 0.0 20.618065 14-15 CAGAGTA 21040 0.0 20.307716 10-11 TGGTATC 3835 0.0 20.066034 2 AGGACCG 1180 0.0 19.322847 5 >>END_MODULE