FastQCFastQC Report
Thu 26 May 2016
SRR937588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044183
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101020.33184601582756357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76210.25034631623657316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.16858382035508376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41660.13685116827733418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.049.849881
GTATCAA102000.043.446231
ATCAACG125650.034.7578773
TCAACGC127750.034.152134
TATCAAC130300.033.6633642
CAACGCA129350.033.655095
AACGCAG134400.032.4821556
ACGCAGA157050.027.6779047
CGCAGAG157400.027.6767238
GCAGAGT172150.024.8629999
TACATGG106900.024.8504542
GTACATG111200.024.6294121
ACATGGG107000.022.9174423
GAGTACT100300.022.56638312-13
TGGTATC29750.022.3635792
AGTACTT104050.021.34221612-13
GTACTTT106450.020.85915214-15
GGACCGA9200.020.6538376
AGAGTAC158350.020.6231310-11
CAGAGTA165200.020.51558710-11
Produced by FastQC (version 0.11.5)