FastQCFastQC Report
Thu 26 May 2016
SRR937588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044183
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129420.4251386989546949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104090.3419308234754612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.22715454360003978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65100.051.3179171
GTATCAA113950.041.5374371
ATCAACG144150.032.6583
TCAACGC147450.031.92714
TATCAAC149450.031.5634082
CAACGCA150600.031.2277575
AACGCAG154500.030.4662256
ACGCAGA176150.026.482097
CGCAGAG175800.026.426738
AGGACCG9800.023.2672865
GCAGAGT196000.023.218439
GGACCGA9050.023.0928546
TATAGCG1050.00219596222.6209725
GAGTACT121600.022.48201212-13
GTACATG105500.021.891651
TACATGG102050.021.5059952
GTACTTT126700.021.42461814-15
CAGAGTA184500.020.97095110-11
AGTACTT126900.020.94410912-13
AGAGTAC178650.020.84665910-11
Produced by FastQC (version 0.11.5)