##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937588_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3044183 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29799982458348 33.0 31.0 34.0 31.0 34.0 2 32.524275971582526 34.0 31.0 34.0 31.0 34.0 3 32.63595059823933 34.0 31.0 34.0 31.0 34.0 4 36.22784766881623 37.0 35.0 37.0 35.0 37.0 5 36.07562193205862 37.0 35.0 37.0 35.0 37.0 6 36.16521214394798 37.0 36.0 37.0 35.0 37.0 7 36.1318291311659 37.0 35.0 37.0 35.0 37.0 8 36.12179326932711 37.0 35.0 37.0 35.0 37.0 9 37.811743577833525 39.0 38.0 39.0 35.0 39.0 10-11 37.767198785355546 39.0 37.0 39.0 35.0 39.0 12-13 37.690445843761694 39.0 37.0 39.0 35.0 39.0 14-15 38.97147247718025 40.0 38.0 41.0 36.0 41.0 16-17 38.9388454964764 40.0 38.0 41.0 36.0 41.0 18-19 38.91517362786665 40.0 38.0 41.0 35.5 41.0 20-21 38.828508667185915 40.0 38.0 41.0 35.0 41.0 22-23 38.68584198124751 40.0 38.0 41.0 34.5 41.0 24-25 38.639087564709484 40.0 38.0 41.0 34.5 41.0 26-27 38.576025160116856 40.0 38.0 41.0 34.0 41.0 28-29 38.47448067346805 40.0 38.0 41.0 34.0 41.0 30-31 38.554958095489 40.0 38.0 41.0 34.5 41.0 32-33 38.48979414181079 40.0 38.0 41.0 34.0 41.0 34-35 38.36972498039704 40.0 38.0 41.0 34.0 41.0 36-37 38.21947924944065 40.0 38.0 41.0 34.0 41.0 38-39 38.09013485720142 40.0 38.0 41.0 34.0 41.0 40-41 37.92240085435074 40.0 38.0 41.0 33.0 41.0 42-43 37.73084486050937 40.0 37.0 41.0 33.0 41.0 44-45 37.454729232769516 40.0 37.0 41.0 33.0 41.0 46-47 37.135073022876746 40.0 37.0 41.0 32.0 41.0 48-49 36.82911819033218 40.0 37.0 41.0 31.0 41.0 50-51 36.596341777087645 39.5 36.0 41.0 31.0 41.0 52-53 36.3821154969987 39.0 36.0 41.0 30.0 41.0 54-55 36.1841594279976 39.0 36.0 40.5 30.0 41.0 56-57 35.916721662265374 39.0 35.0 40.0 29.0 41.0 58-59 35.667769480349904 38.5 35.0 40.0 29.0 41.0 60-61 35.33562486223725 38.0 35.0 40.0 28.0 41.0 62-63 34.97804928284535 38.0 34.0 40.0 28.0 41.0 64-65 34.58193988337757 37.0 34.0 40.0 27.0 41.0 66-67 34.166108607793944 37.0 34.0 39.0 26.0 41.0 68-69 33.71514754533482 36.0 33.0 39.0 26.0 40.0 70-71 33.310080241562346 36.0 33.0 39.0 25.5 40.0 72-73 32.86084098754904 35.0 32.0 38.0 25.0 40.0 74-75 32.37010373555072 35.0 32.0 37.0 23.5 39.0 76-77 31.126585359684356 34.0 30.5 36.0 22.5 38.5 78-79 31.68658093156686 34.5 31.0 36.0 23.0 39.0 80-81 31.48615654840724 35.0 31.0 36.0 22.5 37.5 82-83 31.129774885412605 34.0 31.0 35.5 20.5 37.0 84-85 30.73607467093798 34.0 31.0 35.0 19.5 37.0 86-87 30.315525380701487 34.0 30.5 35.0 18.0 36.0 88-89 29.889878499420043 34.0 30.0 35.0 14.5 36.0 90-91 29.386955383431285 34.0 29.5 35.0 7.0 35.0 92-93 28.93664523453419 34.0 29.0 35.0 2.0 35.0 94-95 28.477036860136202 34.0 29.0 35.0 2.0 35.0 96-97 27.944259428556038 33.0 28.5 35.0 2.0 35.0 98-99 27.14565895020109 33.0 26.5 35.0 2.0 35.0 100-101 24.677525956882356 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17533835135419906 1101 2 0.19686437797729894 1101 3 0.15935286567131612 1101 4 0.06668803444620153 1101 5 -0.07208307776063094 1101 6 0.0517703318727456 1101 7 0.04346675450062065 1101 8 0.03470921444092312 1101 9 0.19938435925843123 1101 10-11 0.1682297161445021 1101 12-13 0.17329290983580137 1101 14-15 0.2218353932594752 1101 16-17 0.009275295802133598 1101 18-19 0.21010288712530212 1101 20-21 0.18743276325933778 1101 22-23 0.21439868491832215 1101 24-25 0.15894567779726998 1101 26-27 0.13159817184361344 1101 28-29 0.19304986437413874 1101 30-31 0.229408296740381 1101 32-33 0.14470447901873484 1101 34-35 0.10378888481129422 1101 36-37 0.14210118169366126 1101 38-39 0.16080150760654277 1101 40-41 0.08870502552300508 1101 42-43 0.1000258361596309 1101 44-45 0.16186295998769396 1101 46-47 0.10121960619135706 1101 48-49 0.2212740163020186 1101 50-51 0.25545789253622075 1101 52-53 0.24998478681651193 1101 54-55 0.2719214149785216 1101 56-57 0.27861318882964525 1101 58-59 0.27679380675104426 1101 60-61 0.26294506263777606 1101 62-63 0.33378153556911627 1101 64-65 0.240659347634832 1101 66-67 0.2601261469695473 1101 68-69 0.12294976184840323 1101 70-71 0.18656857224924295 1101 72-73 0.1863701838580738 1101 74-75 0.19300109637038787 1101 76-77 0.11318088870515908 1101 78-79 0.14414746109360976 1101 80-81 0.23106182292395516 1101 82-83 0.0859106593453518 1101 84-85 0.23176835148418107 1101 86-87 0.4381209227093734 1101 88-89 0.5698724998601392 1101 90-91 0.5147310760902855 1101 92-93 0.7601761935838525 1101 94-95 0.7786278058309755 1101 96-97 0.9487998205812858 1101 98-99 1.2395743344553196 1101 100-101 1.9023676741052888 1102 1 0.16885607266028302 1102 2 0.2344110391170986 1102 3 0.13916045212779693 1102 4 0.07175211795087222 1102 5 0.022183481597117805 1102 6 0.01973003227296033 1102 7 0.06854698599604347 1102 8 0.08819531770993905 1102 9 0.23828012982137636 1102 10-11 0.15496686320582853 1102 12-13 0.1770034449954494 1102 14-15 0.2684728050096723 1102 16-17 0.23052811128922457 1102 18-19 0.24801079579908247 1102 20-21 0.2209251552700522 1102 22-23 0.23466970773309015 1102 24-25 0.19494162175257657 1102 26-27 0.1519465933145554 1102 28-29 0.05599920029528249 1102 30-31 0.23065498812941598 1102 32-33 0.21582908230017495 1102 34-35 0.24367327591550492 1102 36-37 0.29105703427496366 1102 38-39 0.2489714902285769 1102 40-41 0.2269856745030694 1102 42-43 0.32256384059467536 1102 44-45 0.3370895629146702 1102 46-47 0.43318252327649276 1102 48-49 0.5341561872996223 1102 50-51 0.5518190419296758 1102 52-53 0.33564387309591837 1102 54-55 0.36984669517133995 1102 56-57 0.48189200251727726 1102 58-59 0.4185947629109563 1102 60-61 0.6159592035901724 1102 62-63 0.5739712760745874 1102 64-65 0.4828225521038405 1102 66-67 0.5713114391910352 1102 68-69 0.4908677848074774 1102 70-71 0.49539332651860235 1102 72-73 0.5213998310138237 1102 74-75 0.5575466686023063 1102 76-77 0.45567664106045314 1102 78-79 0.6409909840931967 1102 80-81 0.6283680914241678 1102 82-83 0.6647850552887924 1102 84-85 0.7210515027248654 1102 86-87 0.8018718472157396 1102 88-89 0.7871490098280205 1102 90-91 1.023396497276174 1102 92-93 1.1456850183219807 1102 94-95 1.081166401046172 1102 96-97 1.3474476926912615 1102 98-99 1.4565101773452476 1102 100-101 2.2743760425222455 1103 1 0.1690808502487684 1103 2 0.2034587708351907 1103 3 0.12298829615581042 1103 4 0.06843763438724437 1103 5 -0.036234044745235394 1103 6 0.05672171944564752 1103 7 0.10751336400096534 1103 8 0.09623703169820175 1103 9 0.14531635844778634 1103 10-11 0.0862078425262851 1103 12-13 0.12430221976042333 1103 14-15 0.1903009763454051 1103 16-17 0.19043488774199346 1103 18-19 0.18350258550190546 1103 20-21 0.20728716943498426 1103 22-23 0.1952680696171143 1103 24-25 0.2179576637741576 1103 26-27 0.16643883257255965 1103 28-29 0.18429199479334812 1103 30-31 0.15206219442004 1103 32-33 0.1784824831456504 1103 34-35 0.18374293725060653 1103 36-37 0.12165877768148903 1103 38-39 0.07760199333602458 1103 40-41 0.13590441543309595 1103 42-43 0.32106975973783847 1103 44-45 0.0944308186721301 1103 46-47 0.15729958113502818 1103 48-49 0.19507303339401005 1103 50-51 0.25353326680190236 1103 52-53 0.07052437620336605 1103 54-55 0.13271743031236127 1103 56-57 0.2592429000502463 1103 58-59 0.18635515000162428 1103 60-61 0.07410103233331 1103 62-63 0.20120105989375503 1103 64-65 0.19105532704232076 1103 66-67 0.18151900354840933 1103 68-69 -0.001330559725303715 1103 70-71 0.07189780826823267 1103 72-73 0.12268283782813683 1103 74-75 0.2200823076804781 1103 76-77 0.19993273568787373 1103 78-79 0.17389724000839735 1103 80-81 0.29100207069731354 1103 82-83 0.24242624124689627 1103 84-85 0.2893619540940371 1103 86-87 0.5058215246401865 1103 88-89 0.6014575821354242 1103 90-91 0.6446344302687947 1103 92-93 0.9119232273572493 1103 94-95 0.8173620502196179 1103 96-97 0.9827014448996039 1103 98-99 1.1669485823708037 1103 100-101 2.0328483337551297 1104 1 0.16640991007783157 1104 2 0.20869381357023542 1104 3 0.10500396567147874 1104 4 0.07105515575476318 1104 5 -0.02849722138341093 1104 6 0.015500209474140547 1104 7 0.1136476232602206 1104 8 0.11284137642754644 1104 9 0.1822955251144549 1104 10-11 0.14185465520434803 1104 12-13 0.15687210936156504 1104 14-15 0.17453797776990854 1104 16-17 0.05571266551977061 1104 18-19 0.08474012111443585 1104 20-21 0.09229874350906186 1104 22-23 0.18782059810715168 1104 24-25 0.09368772075421106 1104 26-27 0.053867607501338455 1104 28-29 0.1135298865312464 1104 30-31 0.14542490809525077 1104 32-33 0.12632792474109777 1104 34-35 0.1355992406694071 1104 36-37 0.11752994933391392 1104 38-39 0.002519639347418945 1104 40-41 -0.027263319609637904 1104 42-43 0.015394011874590774 1104 44-45 0.04639395969777382 1104 46-47 0.08431168939713274 1104 48-49 0.10590592157064549 1104 50-51 0.018259050277691813 1104 52-53 0.1163999994227396 1104 54-55 0.09577165039782898 1104 56-57 0.2183864185687696 1104 58-59 0.2336753066967887 1104 60-61 0.11561411994014037 1104 62-63 0.20361158339802898 1104 64-65 0.12647199370898932 1104 66-67 0.10257936679627022 1104 68-69 0.07989718243993593 1104 70-71 0.23447125912294098 1104 72-73 0.3447893193096192 1104 74-75 0.39517801636993255 1104 76-77 0.07670223355112071 1104 78-79 0.3054677528289069 1104 80-81 0.3650716932044311 1104 82-83 0.3811838089107127 1104 84-85 0.5129619362877698 1104 86-87 0.5710948508510114 1104 88-89 0.6946600394003823 1104 90-91 0.6261159117502757 1104 92-93 0.9308245806336011 1104 94-95 0.9875788415301621 1104 96-97 1.3083224384893484 1104 98-99 1.5055013779548467 1104 100-101 2.186149170649717 1105 1 0.18797382410497931 1105 2 0.14916274687004716 1105 3 0.09224891707084026 1105 4 0.03756208972493624 1105 5 -0.10040207306767712 1105 6 0.029931142103599484 1105 7 0.06625519406632208 1105 8 0.07315696633872903 1105 9 0.15084808502730596 1105 10-11 0.11620682128445736 1105 12-13 0.08242279437915556 1105 14-15 0.18650536087699976 1105 16-17 0.1515766361080111 1105 18-19 0.19371500380255924 1105 20-21 0.13168123261581144 1105 22-23 0.13395951841886244 1105 24-25 0.0920385461270925 1105 26-27 0.044094061330731904 1105 28-29 0.08458475078463579 1105 30-31 0.15310045694727137 1105 32-33 0.08119217813476354 1105 34-35 0.13627221284972535 1105 36-37 0.1275394285529572 1105 38-39 -0.08052879370101351 1105 40-41 -0.006133302431827303 1105 42-43 0.05408310396441607 1105 44-45 0.10636232738672646 1105 46-47 0.2046171192613926 1105 48-49 0.09224249145501773 1105 50-51 0.15595360993916074 1105 52-53 0.18154187348005735 1105 54-55 0.15429116397249487 1105 56-57 0.1453574153007935 1105 58-59 0.16761536241993014 1105 60-61 0.24079323004572473 1105 62-63 0.23965932514430222 1105 64-65 0.21520769632336822 1105 66-67 0.23947447740462025 1105 68-69 0.19563294789996633 1105 70-71 0.2921200985732497 1105 72-73 0.3359181319457818 1105 74-75 0.4463639661772092 1105 76-77 0.2945082395005123 1105 78-79 0.3824858837992444 1105 80-81 0.5763881001090745 1105 82-83 0.5544241306804487 1105 84-85 0.4884445385166991 1105 86-87 0.6944619113504267 1105 88-89 0.8233223697171219 1105 90-91 0.7677961949746788 1105 92-93 1.100972110799514 1105 94-95 1.0465202065377035 1105 96-97 1.02366175263381 1105 98-99 1.4824357147237421 1105 100-101 1.8272127809228884 1106 1 -0.1562083318952503 1106 2 0.0466147934266985 1106 3 -0.05766908409834315 1106 4 0.019341158879477405 1106 5 -0.0980738812826516 1106 6 -0.09845572706244354 1106 7 -0.05659241149594152 1106 8 -0.08200201196422796 1106 9 0.048853578769282535 1106 10-11 -0.04384393533295139 1106 12-13 -0.22188945165806473 1106 14-15 -0.07910765974670397 1106 16-17 -0.03938345699570789 1106 18-19 -0.02912265887737675 1106 20-21 -0.09084451705355434 1106 22-23 6.587970727167658E-5 1106 24-25 -0.008306998982440916 1106 26-27 -0.15058218404523416 1106 28-29 -0.06347465015070952 1106 30-31 -0.10978935195771555 1106 32-33 -0.05600282694449277 1106 34-35 -0.23961276357486128 1106 36-37 0.007895557441457868 1106 38-39 -0.1996229611822784 1106 40-41 -0.14646735332980398 1106 42-43 -0.0633583588132609 1106 44-45 -0.01685933420068153 1106 46-47 0.009263440155343972 1106 48-49 0.04149381038434541 1106 50-51 -0.014662806837712594 1106 52-53 7.672450803113406E-4 1106 54-55 -0.020550378827429938 1106 56-57 -0.13716913698679178 1106 58-59 -0.13343040718311983 1106 60-61 -0.07538144363606847 1106 62-63 -0.08604344496667693 1106 64-65 -0.20847424285015137 1106 66-67 -0.029550868559773846 1106 68-69 -0.22964614161951147 1106 70-71 -0.03297950994073062 1106 72-73 0.06316121026215171 1106 74-75 0.09375476817249506 1106 76-77 -0.007725584747664271 1106 78-79 0.18777594642742557 1106 80-81 0.11788226375693966 1106 82-83 0.018320686370174144 1106 84-85 0.00949634572540603 1106 86-87 0.24198948787467955 1106 88-89 0.34868540471606835 1106 90-91 0.3694633647751502 1106 92-93 0.5098768903310749 1106 94-95 0.4948726230174927 1106 96-97 0.7144014252134951 1106 98-99 0.7776918517582239 1106 100-101 1.4273473392672393 1107 1 0.11250197062772571 1107 2 0.07914313221232305 1107 3 0.08861361795582923 1107 4 0.06415233077964899 1107 5 -0.19703805137002917 1107 6 -0.017297648624925444 1107 7 0.03725315650431327 1107 8 0.017931823686673454 1107 9 0.1072122861757947 1107 10-11 0.06450766038747702 1107 12-13 0.15755689980538534 1107 14-15 0.15594051097966855 1107 16-17 0.07902128461152103 1107 18-19 0.08214684201513478 1107 20-21 0.08974837107916755 1107 22-23 0.10852392423215917 1107 24-25 0.13851489268981965 1107 26-27 0.010964370622190245 1107 28-29 -0.08803019996860684 1107 30-31 0.0069547882998293176 1107 32-33 0.015438212044152522 1107 34-35 0.08938017637805018 1107 36-37 0.08163422970325485 1107 38-39 -0.07620380142421368 1107 40-41 0.00414607866722605 1107 42-43 0.09671588054585811 1107 44-45 -0.00519873587082742 1107 46-47 0.02539911602673328 1107 48-49 -0.13552647468102208 1107 50-51 -0.11450619875261481 1107 52-53 -0.0927133771605142 1107 54-55 -0.05541772247828902 1107 56-57 0.13164972821761722 1107 58-59 0.09276122301161394 1107 60-61 0.10645440415392926 1107 62-63 0.02775259187638568 1107 64-65 0.0866151303581546 1107 66-67 0.1531611812335072 1107 68-69 0.21540845831266608 1107 70-71 0.29324138235089237 1107 72-73 0.30539928052198917 1107 74-75 0.3244352161090589 1107 76-77 0.2786982518121981 1107 78-79 0.2453143185790907 1107 80-81 0.17399799670905125 1107 82-83 0.2541165655784141 1107 84-85 0.2586661205461702 1107 86-87 0.4651720142052831 1107 88-89 0.5800940832681576 1107 90-91 0.6284157092307971 1107 92-93 0.7806701166237175 1107 94-95 0.9486539110389671 1107 96-97 1.0600816119352672 1107 98-99 1.328236763943245 1107 100-101 2.049920495264413 1108 1 0.07131243576687041 1108 2 0.11281686488429443 1108 3 0.042541979052188594 1108 4 0.029358878665227905 1108 5 -0.09534635227748822 1108 6 -0.021136686713319364 1108 7 0.045149912065880926 1108 8 -0.022462250449109433 1108 9 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-0.4338901784253011 2316 68-69 -0.4467059011323471 2316 70-71 -0.46373772470192876 2316 72-73 -0.4758196608212444 2316 74-75 -0.4619807381956278 2316 76-77 -0.35739668271959246 2316 78-79 -0.5427480418212447 2316 80-81 -0.537405715634403 2316 82-83 -0.6015289312574552 2316 84-85 -0.6312808384655391 2316 86-87 -0.6620911839557699 2316 88-89 -0.7445786257702132 2316 90-91 -0.7704790219192112 2316 92-93 -0.8876342565853612 2316 94-95 -1.034477843006261 2316 96-97 -1.0269594835024591 2316 98-99 -1.0751988210597752 2316 100-101 -1.6652120709060156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 27.0 5 38.0 6 81.0 7 202.0 8 531.0 9 1097.0 10 2018.0 11 3313.0 12 5310.0 13 7752.0 14 10200.0 15 14071.0 16 19049.0 17 27175.0 18 24971.0 19 15122.0 20 12557.0 21 12660.0 22 13133.0 23 14377.0 24 16580.0 25 19908.0 26 24402.0 27 30451.0 28 37969.0 29 48582.0 30 61762.0 31 78812.0 32 102849.0 33 137441.0 34 193015.0 35 285002.0 36 458343.0 37 728335.0 38 586089.0 39 50948.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42966292164372 19.514388164119357 15.417188238916843 22.638760675320082 2 15.274771588961636 20.143467064890647 40.9697117420339 23.612049604113814 3 19.37849991278448 24.023851391325685 30.163298329962423 26.43435036592741 4 12.895943803622782 16.935058416349612 37.19192873487853 32.97706904514908 5 13.609614617604022 37.50383568862684 34.483218574786235 14.403331118982898 6 32.317636620400286 36.477570500853595 17.13451523775016 14.07027764099596 7 28.633528273431658 32.99499405916136 20.301473334553148 18.07000433285384 8 26.16232335572467 35.87809931268915 19.366476982494156 18.593100349092023 9 26.058321723759708 17.142826170437193 20.284424425207025 36.51442768059607 10-11 24.772820819247727 27.15925750850064 27.151784238989574 20.91613743326206 12-13 25.57271932285483 24.145585816901953 28.07663348525693 22.205061374986286 14-15 22.48895680713019 25.618532131609694 26.48239281278425 25.410118248475865 16-17 21.786715844612495 29.018179918881355 27.11908252559061 22.07602171091554 18-19 21.61430505327702 27.47321695180612 29.729980096465948 21.182497898450915 20-21 22.241972103174533 26.449474185425544 30.184665401849397 21.12388830955053 22-23 22.052568576849694 26.3311812457624 29.959880769048837 21.65636940833907 24-25 21.90665483199985 26.425777323429354 30.16928457205328 21.49828327251751 26-27 21.65353153794175 26.84589046383413 30.002427625391448 21.498150372832672 28-29 21.599033938621858 26.946223743993734 30.024739554674873 21.43000276270953 30-31 21.74479558040088 26.714348011402134 29.93496579935237 21.605890608844618 32-33 21.618559068229473 26.57793568914878 29.99445171331684 21.809053529304904 34-35 22.011308124380168 26.697294479339778 29.853789998827274 21.437607397452783 36-37 21.96465618098001 26.602290303649767 29.807404716177405 21.62564879919282 38-39 21.753866308300125 26.87151199517243 29.872645632670576 21.50197606385687 40-41 22.075299021116667 26.73025898902924 29.625420022383675 21.569021967470416 42-43 21.9338576623991 26.6826823571154 29.572898495017892 21.81056148546761 44-45 21.943605230040376 26.951697713310928 29.45295995674373 21.651737099904967 46-47 22.00309574030208 27.17548517943895 28.96622837720334 21.85519070305563 48-49 22.10966326756034 27.28060817641518 28.79820772900442 21.81152082702006 50-51 22.275007777127723 27.4837945024987 28.51109805159545 21.730099668778124 52-53 22.296967692152542 27.483351033758485 28.378008155225885 21.841673118863092 54-55 22.244884752329277 27.465497310772708 28.47158005941167 21.81803787748634 56-57 22.151394971984274 27.681729383548888 28.43909186799874 21.727783776468105 58-59 22.186133356634606 27.434947242002206 28.524763458701397 21.85415594266179 60-61 22.203297239357816 27.498543944302956 28.530117933120312 21.768040883218916 62-63 22.06711948657489 27.480328876417744 28.72504708159792 21.72750455540945 64-65 22.1028762068509 27.339617887623707 28.621965236649704 21.93554066887569 66-67 22.04548806691319 27.450747868968456 28.506778337570378 21.996985726547976 68-69 22.14960467225525 27.399502592321156 28.55399954601941 21.896893189404185 70-71 22.18383388909274 27.310431074610165 28.4291713080324 22.076563728264695 72-73 22.164859556550386 27.288917635920885 28.356775340740477 22.189447466788252 74-75 22.081768462519147 27.586270330180763 28.07280513215213 22.259156075147963 76-77 22.163368627970133 27.48929679983102 28.26574486487836 22.081589707320486 78-79 22.234027980578038 27.492532479157788 28.20315007343514 22.07028946682903 80-81 22.13488807998731 27.631535292063585 28.09331764877473 22.14025897917438 82-83 22.21272504224046 27.585898790100515 28.15000828630778 22.05136788135124 84-85 22.17062822295966 27.545255297496134 28.0690591273692 22.21505735217501 86-87 22.1442994721408 27.510895369956405 28.111204221296816 22.23360093660598 88-89 22.248843121454918 27.466203575803426 28.09157662335017 22.193376679391484 90-91 22.21815406732265 27.428146419410453 28.14510533619276 22.208594177074133 92-93 22.058327390129016 27.30003649691386 28.219670029792127 22.421966083165003 94-95 21.925833630895383 27.446756650306504 28.246462187063 22.380947531735114 96-97 22.01828865084655 27.302809982185693 28.170185563745676 22.508715803222078 98-99 21.865526478351857 27.179149165348715 28.160170134737633 22.795154221561802 100-101 21.840656858599765 27.327822496789395 28.28173864363246 22.549782000978375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 174.5 2 172.5 3 251.0 4 241.5 5 250.0 6 503.5 7 1154.5 8 2022.5 9 2611.5 10 2676.5 11 2682.0 12 2965.5 13 3271.5 14 3625.5 15 3914.5 16 4084.0 17 4385.0 18 4755.5 19 5250.0 20 5859.0 21 6584.0 22 7716.5 23 9548.5 24 11915.0 25 15071.0 26 19284.0 27 23961.0 28 27878.0 29 32335.0 30 38265.5 31 44124.0 32 50501.5 33 56974.5 34 63625.5 35 69949.5 36 77171.5 37 84058.5 38 88660.0 39 92279.5 40 95785.5 41 100956.5 42 104461.0 43 107751.0 44 111881.5 45 113557.0 46 115101.5 47 118928.5 48 121675.5 49 119909.5 50 116471.5 51 111150.0 52 104996.5 53 101659.5 54 99379.0 55 90863.5 56 78549.0 57 69149.0 58 61987.0 59 53624.0 60 43261.5 61 34193.5 62 26448.5 63 20045.5 64 14469.0 65 10154.5 66 7337.0 67 5528.5 68 4178.0 69 3059.0 70 2163.0 71 1370.0 72 967.0 73 735.5 74 527.0 75 385.5 76 259.0 77 147.0 78 92.5 79 54.0 80 37.5 81 24.5 82 13.0 83 8.0 84 6.5 85 5.0 86 4.5 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04270439720608124 2 0.0 3 0.0 4 3.284953631237018E-5 5 0.013172664061260445 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035805994580483495 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.927430446855527E-5 22-23 2.299467541865913E-4 24-25 6.241411899350335E-4 26-27 0.0018395740334927304 28-29 0.0022994675418659127 30-31 5.091678128417379E-4 32-33 0.0 34-35 0.0 36-37 4.927430446855527E-5 38-39 0.0 40-41 0.0 42-43 0.009624914139524463 44-45 0.0 46-47 0.0 48-49 1.642476815618509E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3139814524948073E-4 74-75 6.569907262474036E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.478229134056658E-4 84-85 2.956458268113316E-4 86-87 0.0 88-89 0.0 90-91 0.00706265030715959 92-93 0.0029564582681133166 94-95 0.0 96-97 0.0 98-99 2.4637152234277635E-4 100-101 0.011891532145078007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3044183.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.17444342613111 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46012939637566 35.35297776196328 2 15.607633679031952 15.662086660887342 3 5.813587132169889 8.750804959978256 4 2.795579502312879 5.6106658232819715 5 1.50346075305727 3.771765324884022 6 0.928807102431616 2.7961427648846326 7 0.5935379216852062 2.0846304411000474 8 0.4253843092330693 1.7074736766382794 9 0.29965592381650574 1.3531562277152689 >10 1.4175904396331156 12.79932543683431 >50 0.10093787898633277 3.49659605605738 >100 0.050939403070600166 4.5995124158376655 >500 0.0022971318300633466 0.780061481119431 >1k 2.625293519116057E-4 0.2381216045423061 >5k 6.563233797790143E-5 0.22771551589130778 >10k+ 1.3126467595580286E-4 0.7689638483843714 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12942 0.4251386989546949 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10409 0.3419308234754612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6915 0.22715454360003978 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.642476815618509E-5 0.0 18-19 0.0 0.0 0.0 3.284953631237018E-5 0.0 20-21 0.0 0.0 0.0 4.927430446855527E-5 0.0 22-23 0.0 0.0 0.0 8.212384078092546E-5 0.0 24-25 0.0 0.0 0.0 2.135219860304062E-4 0.0 26-27 0.0 0.0 0.0 4.106192039046273E-4 0.0 28-29 0.0 0.0 0.0 7.22689798872144E-4 0.0 30-31 0.0 0.0 0.0 0.0013796805251195476 0.0 32-33 0.0 0.0 0.0 0.0025951133686772446 0.0 34-35 0.0 0.0 0.0 0.004155466343514828 0.0 36-37 0.0 0.0 0.0 0.006093588985944668 0.0 38-39 0.0 0.0 0.0 0.008031711628374509 0.0 40-41 0.0 0.0 0.0 0.011103143273581122 0.0 42-43 0.0 0.0 0.0 0.01516006100815884 0.0 44-45 0.0 0.0 0.0 0.021598570125383396 0.0 46-47 0.0 0.0 0.0 0.03628231285701287 0.0 48-49 0.0 0.0 0.0 0.0644507902448703 0.0 50-51 0.0 0.0 0.0 0.08657495295125162 0.0 52-53 0.0 0.0 0.0 0.10948750452912982 0.0 54-55 0.0 0.0 0.0 0.14087523647559952 0.0 56-57 0.0 0.0 0.0 0.16377136328532155 0.0 58-59 0.0 0.0 0.0 0.18931187776818936 0.0 60-61 0.0 0.0 0.0 0.23314958397704738 0.0 62-63 0.0 0.0 0.0 0.2648658112866408 0.0 64-65 0.0 0.0 0.0 0.3000969389816578 0.0 66-67 0.0 0.0 0.0 0.36799693053932697 0.0 68-69 0.0 0.0 0.0 0.4471971625884515 0.0 70-71 0.0 0.0 0.0 0.5156391714952747 0.0 72-73 0.0 0.0 0.0 0.5976151893627946 0.0 74-75 0.0 0.0 0.0 0.6780965533281015 0.0 76-77 0.0 0.0 0.0 0.747359800642734 0.0 78-79 0.0 0.0 0.0 0.8332941876358945 0.0 80-81 0.0 0.0 0.0 0.9642652889133143 0.0 82-83 0.0 0.0 0.0 1.0810453905037902 0.0 84-85 0.0 0.0 0.0 1.210029094834312 0.0 86-87 0.0 0.0 0.0 1.4342600297025507 0.0 88-89 0.0 0.0 0.0 1.7298073079049452 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6510 0.0 51.317917 1 GTATCAA 11395 0.0 41.537437 1 ATCAACG 14415 0.0 32.658 3 TCAACGC 14745 0.0 31.9271 4 TATCAAC 14945 0.0 31.563408 2 CAACGCA 15060 0.0 31.227757 5 AACGCAG 15450 0.0 30.466225 6 ACGCAGA 17615 0.0 26.48209 7 CGCAGAG 17580 0.0 26.42673 8 AGGACCG 980 0.0 23.267286 5 GCAGAGT 19600 0.0 23.21843 9 GGACCGA 905 0.0 23.092854 6 TATAGCG 105 0.002195962 22.620972 5 GAGTACT 12160 0.0 22.482012 12-13 GTACATG 10550 0.0 21.89165 1 TACATGG 10205 0.0 21.505995 2 GTACTTT 12670 0.0 21.424618 14-15 CAGAGTA 18450 0.0 20.970951 10-11 AGTACTT 12690 0.0 20.944109 12-13 AGAGTAC 17865 0.0 20.846659 10-11 >>END_MODULE