FastQCFastQC Report
Thu 26 May 2016
SRR937587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895751
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151770.8005798229830816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119800.6319395321431981No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82280.434023244613876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.1408940309143975No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24700.13029137265389812No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24440.1289198845206992No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22640.11942496667547585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.1058683339742403No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19380.10222861546690468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114100.032.218651
GTATCAA165700.029.8199211
GTACATG131800.025.1856691
TACATGG133450.024.5101222
ATCAACG203550.024.1510773
TATCAAC205350.024.0082042
TCAACGC205600.023.9109044
CAACGCA205900.023.8760665
AACGCAG211550.023.3291686
GAGTACT106850.023.02686312-13
ACATGGG134700.022.8361973
AGTACTT111200.022.19015312-13
CATGGGA98750.021.0880094
GTACTTT117150.021.00203114-15
ACGCAGA235400.020.8848157
CGCAGAG236050.020.8066318
GCAGAGT240050.019.747599
GTGTAGC9950.019.5963061
ATGGGCG3203.929017E-1019.3148775
GACCGAC1507.7232014E-419.000147
Produced by FastQC (version 0.11.5)