##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937587_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1895751 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75653026162191 33.0 31.0 34.0 30.0 34.0 2 31.878301000500592 33.0 31.0 34.0 30.0 34.0 3 31.87840175212884 33.0 31.0 34.0 30.0 34.0 4 35.49710114883231 37.0 35.0 37.0 33.0 37.0 5 35.502874586377644 37.0 35.0 37.0 33.0 37.0 6 35.58991027830132 37.0 35.0 37.0 33.0 37.0 7 35.532762477772664 37.0 35.0 37.0 33.0 37.0 8 35.531254895817014 37.0 35.0 37.0 33.0 37.0 9 37.15347110459127 39.0 37.0 39.0 34.0 39.0 10-11 37.02876538110754 39.0 37.0 39.0 33.0 39.0 12-13 36.91861457543739 39.0 37.0 39.0 33.0 39.0 14-15 38.018442822923475 40.0 38.0 41.0 33.0 41.0 16-17 37.952527784503346 40.0 38.0 41.0 33.0 41.0 18-19 37.9633622770079 40.0 38.0 41.0 33.0 41.0 20-21 37.90182940692105 40.0 38.0 41.0 33.0 41.0 22-23 37.821996665173856 40.0 38.0 41.0 33.0 41.0 24-25 37.846945616803055 40.0 38.0 41.0 33.0 41.0 26-27 37.762564281912546 40.0 37.0 41.0 33.0 41.0 28-29 37.640295323594714 40.0 37.0 41.0 32.5 41.0 30-31 37.37021713294626 39.5 37.0 41.0 32.0 41.0 32-33 37.173353726306885 39.0 36.0 41.0 32.0 41.0 34-35 37.12924350297059 40.0 36.0 41.0 31.5 41.0 36-37 36.980703425713614 39.5 36.0 41.0 31.0 41.0 38-39 36.811021331387934 39.0 36.0 41.0 31.0 41.0 40-41 36.565242481739425 39.0 35.0 41.0 30.5 41.0 42-43 36.26347473903482 39.0 35.0 41.0 30.0 41.0 44-45 35.941674565910816 39.0 35.0 41.0 29.5 41.0 46-47 35.464405399232284 39.0 35.0 40.5 26.5 41.0 48-49 35.045471689056214 39.0 35.0 40.0 24.5 41.0 50-51 34.12968000544375 38.0 34.0 39.5 23.5 40.5 52-53 34.21376541539475 38.0 34.0 39.5 23.0 40.5 54-55 34.50750098509773 38.0 34.0 40.0 23.0 41.0 56-57 34.30958179634351 38.0 34.0 40.0 22.5 41.0 58-59 34.07837368937165 38.0 34.0 40.0 21.5 41.0 60-61 33.77331674887683 38.0 34.0 40.0 19.5 41.0 62-63 33.42376161215265 37.5 33.0 40.0 18.5 41.0 64-65 33.05451269707889 37.0 33.0 40.0 16.0 41.0 66-67 32.62642878732492 36.5 32.0 39.0 12.5 41.0 68-69 32.1377374982263 36.0 31.5 39.0 8.0 40.0 70-71 31.695947410815027 35.0 31.0 39.0 7.0 40.0 72-73 31.246049191059374 35.0 31.0 38.0 4.0 40.0 74-75 30.717640396866464 35.0 30.0 37.0 2.0 39.0 76-77 30.232660565654456 34.5 30.0 37.0 2.0 39.0 78-79 29.672755018987196 34.0 29.0 36.0 2.0 39.0 80-81 29.116364174408982 34.0 29.0 36.0 2.0 37.5 82-83 28.610413498397207 34.0 28.0 35.0 2.0 37.0 84-85 28.095572414309686 34.0 27.0 35.0 2.0 36.5 86-87 27.56065933764508 33.0 26.0 35.0 2.0 36.0 88-89 27.102494473166573 33.0 26.0 35.0 2.0 36.0 90-91 26.556435417942545 33.0 25.0 35.0 2.0 35.0 92-93 25.943518426206815 32.0 23.5 35.0 2.0 35.0 94-95 25.22907676166332 32.0 19.5 34.0 2.0 35.0 96-97 24.415748692734436 31.5 12.5 34.0 2.0 35.0 98-99 23.393000979559027 31.0 2.0 34.0 2.0 35.0 100-101 20.639988848746487 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18788996817856685 1101 2 0.3190723336952068 1101 3 0.36806903863076457 1101 4 0.17133638791537464 1101 5 0.1147143403034363 1101 6 0.1805430418104379 1101 7 0.2400538099012195 1101 8 0.26961655550811514 1101 9 0.410261000225546 1101 10-11 0.38305134528983587 1101 12-13 0.3633319807486117 1101 14-15 0.4595800986266667 1101 16-17 0.5624861791586184 1101 18-19 0.5621656158987562 1101 20-21 0.5804595717484418 1101 22-23 0.5636009006616689 1101 24-25 0.4991471312598392 1101 26-27 0.3703478150980999 1101 28-29 0.33510602131252654 1101 30-31 0.5113378676167315 1101 32-33 0.5040032576534799 1101 34-35 0.3312475603263394 1101 36-37 0.5350182783222408 1101 38-39 0.5142640791722854 1101 40-41 0.5357715869965745 1101 42-43 0.5613960216807499 1101 44-45 0.42618632154627534 1101 46-47 0.4309397685025047 1101 48-49 0.5852380100712438 1101 50-51 0.4547950860120409 1101 52-53 0.40215459173201396 1101 54-55 0.5491519330442998 1101 56-57 0.3381214671360979 1101 58-59 0.4643446102057922 1101 60-61 0.6893763681422627 1101 62-63 0.8231935507251436 1101 64-65 0.8295538465684373 1101 66-67 0.8506443536677608 1101 68-69 0.6784283194694183 1101 70-71 0.6483380049494514 1101 72-73 0.6684297092985965 1101 74-75 0.636258952918503 1101 76-77 0.6607634292544944 1101 78-79 0.9506510635189294 1101 80-81 0.9398468688653594 1101 82-83 0.9974379515394141 1101 84-85 1.1816656298744768 1101 86-87 1.2968674328872254 1101 88-89 1.1643791900776925 1101 90-91 1.2185202849528771 1101 92-93 1.4253107507041989 1101 94-95 1.5438406526822028 1101 96-97 1.590962433792665 1101 98-99 1.8121970107771226 1101 100-101 2.010780776558722 1102 1 0.08557148347951227 1102 2 0.1642960919573362 1102 3 0.2337693531816143 1102 4 0.0272988207252709 1102 5 0.06513036819592344 1102 6 0.04973159452985243 1102 7 0.10766960833819894 1102 8 0.039194914230272104 1102 9 0.1873933877817251 1102 10-11 0.08718064995688479 1102 12-13 0.15196509905126732 1102 14-15 0.22651558159585505 1102 16-17 0.24866019857735466 1102 18-19 0.26808209051384324 1102 20-21 0.24855362614460574 1102 22-23 0.20658344213866542 1102 24-25 0.206211722092128 1102 26-27 0.10176493771255934 1102 28-29 0.23625145383952884 1102 30-31 0.15111535615234573 1102 32-33 0.19786912458047112 1102 34-35 0.30826986067376794 1102 36-37 0.36239353984225886 1102 38-39 0.27296622422350225 1102 40-41 0.16091988883568575 1102 42-43 -0.01005555280675452 1102 44-45 -0.08555007074661347 1102 46-47 0.022640065162093492 1102 48-49 0.22012139814067666 1102 50-51 0.16814482340177506 1102 52-53 0.23847688503475695 1102 54-55 0.16175733777273393 1102 56-57 0.21944002004561014 1102 58-59 0.10611585692525694 1102 60-61 -0.011737978306491925 1102 62-63 0.10031727219835318 1102 64-65 0.10348677220450497 1102 66-67 0.06070908651198437 1102 68-69 0.2776549694077488 1102 70-71 0.25045996525096825 1102 72-73 0.22320047183562153 1102 74-75 0.1270178334171348 1102 76-77 0.21238952187982818 1102 78-79 0.4413076283283708 1102 80-81 0.4584792267891231 1102 82-83 0.43012121683002036 1102 84-85 0.48026194807515665 1102 86-87 0.5933427971702159 1102 88-89 0.5755753219855428 1102 90-91 0.4789226595714311 1102 92-93 0.41575038388847574 1102 94-95 0.7030667882465771 1102 96-97 0.8559860670395629 1102 98-99 0.9337377061436172 1102 100-101 1.4008422889747045 1103 1 0.10576098720526872 1103 2 0.2563923005765467 1103 3 0.19505793462595022 1103 4 0.15100658955246615 1103 5 0.05176823414194587 1103 6 0.1154105093404354 1103 7 0.15509883574005556 1103 8 0.20389981918319222 1103 9 0.17789494982370968 1103 10-11 0.301697033501803 1103 12-13 0.2942137236757816 1103 14-15 0.2538882839258818 1103 16-17 0.23664875144041986 1103 18-19 0.2883669522134369 1103 20-21 0.3343184571833362 1103 22-23 0.21989567654058106 1103 24-25 0.25916949131119793 1103 26-27 0.27233997503279284 1103 28-29 0.23097436969303686 1103 30-31 0.33893947529286095 1103 32-33 0.2891980280787294 1103 34-35 0.3222225610287026 1103 36-37 0.2294672674925735 1103 38-39 0.26613898517909007 1103 40-41 0.2849593205335097 1103 42-43 0.29099061560535233 1103 44-45 0.18706061112299466 1103 46-47 0.10768291877428027 1103 48-49 0.12134007304626948 1103 50-51 0.007389959230721388 1103 52-53 0.08946960073056687 1103 54-55 0.17200165796443656 1103 56-57 0.3063947357194863 1103 58-59 0.1490769754298782 1103 60-61 0.21275402494770645 1103 62-63 0.2842944155451619 1103 64-65 0.3120583045313836 1103 66-67 0.28059360147430823 1103 68-69 0.24074725345852244 1103 70-71 0.11364439384247405 1103 72-73 0.14081967322173838 1103 74-75 0.08175075010143473 1103 76-77 0.23177187913938724 1103 78-79 0.24642884790721453 1103 80-81 0.28506879196198653 1103 82-83 0.4487796372526134 1103 84-85 0.5276461090373239 1103 86-87 0.44383808387376433 1103 88-89 0.4799298373292906 1103 90-91 0.7258083726479079 1103 92-93 0.5737402587004361 1103 94-95 0.6511618755605149 1103 96-97 0.7985204782053792 1103 98-99 0.6114544025671975 1103 100-101 0.9058818925781402 1104 1 0.18615618496956543 1104 2 0.3129195110820895 1104 3 0.1881622781192931 1104 4 0.07057780371582112 1104 5 0.2559001226325819 1104 6 0.13790997205754962 1104 7 0.25262698090315894 1104 8 0.2807018508322514 1104 9 0.21752786502342758 1104 10-11 0.21467399991297498 1104 12-13 0.3309514985644739 1104 14-15 0.3903860429953525 1104 16-17 0.36309016588717924 1104 18-19 0.26150407059421354 1104 20-21 0.3671208716859127 1104 22-23 0.36822628620954845 1104 24-25 0.32654855341605327 1104 26-27 0.27874658415525744 1104 28-29 0.18588541307241258 1104 30-31 0.38447025396286705 1104 32-33 0.29722582991111324 1104 34-35 0.18381356488021794 1104 36-37 0.30833774355172494 1104 38-39 0.47148522138304116 1104 40-41 0.49314513783107117 1104 42-43 0.33215885926990296 1104 44-45 0.34076682761857313 1104 46-47 0.41426575163270485 1104 48-49 0.46811292852992636 1104 50-51 0.5006776481415329 1104 52-53 0.44854099407240255 1104 54-55 0.47404744936633847 1104 56-57 0.6064910299449338 1104 58-59 0.6141798521598787 1104 60-61 0.6704978307894507 1104 62-63 0.6530621550350659 1104 64-65 0.7431913918384012 1104 66-67 0.7950989767023202 1104 68-69 0.6841803938750957 1104 70-71 0.6813150155677903 1104 72-73 0.6921426265780077 1104 74-75 0.6748227934861113 1104 76-77 0.6265701221549023 1104 78-79 0.7923897054283806 1104 80-81 1.0315802413277169 1104 82-83 1.0283425831942523 1104 84-85 0.9959312662156101 1104 86-87 1.0163677379747007 1104 88-89 1.059678654107369 1104 90-91 1.059611055097431 1104 92-93 1.2237723694026812 1104 94-95 1.4035283825449412 1104 96-97 1.465926966360282 1104 98-99 1.4738886438139076 1104 100-101 1.6148793795337184 1105 1 0.3186268023044967 1105 2 0.21444708259549472 1105 3 0.2005646297545951 1105 4 0.17969151480851053 1105 5 0.19532542949093568 1105 6 0.1586446945537503 1105 7 0.18424501801132465 1105 8 0.22431493486988785 1105 9 0.20586806512910982 1105 10-11 0.2500863844290606 1105 12-13 0.2692955570773492 1105 14-15 0.26760475489039237 1105 16-17 0.369464075695511 1105 18-19 0.4737021566198294 1105 20-21 0.38771058450798535 1105 22-23 0.4441822257547017 1105 24-25 0.45783635175269666 1105 26-27 0.4050330179955921 1105 28-29 0.3713872819724173 1105 30-31 0.3636093027811711 1105 32-33 0.27729728454595914 1105 34-35 0.450369709048708 1105 36-37 0.5628203950287016 1105 38-39 0.6107443356562854 1105 40-41 0.6548374403342834 1105 42-43 0.6660343712115449 1105 44-45 0.3391896078590335 1105 46-47 0.3393878663873551 1105 48-49 0.2788520511434669 1105 50-51 0.2924617705148762 1105 52-53 0.3358949239751112 1105 54-55 0.45257106946194625 1105 56-57 0.46911831905359946 1105 58-59 0.4959508457278474 1105 60-61 0.6021957190397629 1105 62-63 0.4400594250203298 1105 64-65 0.5167234595460357 1105 66-67 0.5785408905540521 1105 68-69 0.5132640707173977 1105 70-71 0.6972138251341846 1105 72-73 0.5942134413816156 1105 74-75 0.6008313197994255 1105 76-77 0.6448616552252844 1105 78-79 0.6653103816870676 1105 80-81 0.7375496827427845 1105 82-83 0.6143358751872654 1105 84-85 0.682757258695041 1105 86-87 0.7558871797475035 1105 88-89 0.8248388725494316 1105 90-91 0.7948465150349016 1105 92-93 1.0541042792989117 1105 94-95 1.2169567638639158 1105 96-97 1.0185119458621728 1105 98-99 1.3238314831723201 1105 100-101 1.602890337410134 1106 1 0.1502191395640935 1106 2 0.14830157275182998 1106 3 0.11135773708998897 1106 4 0.2145681678522351 1106 5 0.11481835977962618 1106 6 0.20243446651494423 1106 7 0.175288245256624 1106 8 0.22025347026707465 1106 9 0.2757457017656222 1106 10-11 0.15054707055686123 1106 12-13 0.0018917668191136272 1106 14-15 0.13335953526185307 1106 16-17 0.11945495812191353 1106 18-19 0.18645106637906395 1106 20-21 0.19037893912302906 1106 22-23 0.3294068938197654 1106 24-25 0.13580973064810564 1106 26-27 0.27979126415017674 1106 28-29 0.3840316988124215 1106 30-31 0.348566964084867 1106 32-33 0.25886762911288486 1106 34-35 0.5142186955060808 1106 36-37 0.3090882682358611 1106 38-39 0.3117796499528609 1106 40-41 0.4270019854612954 1106 42-43 0.5438602664467567 1106 44-45 0.41821714403645416 1106 46-47 0.45921378151545866 1106 48-49 0.4441782502977958 1106 50-51 0.5185905813843448 1106 52-53 0.46302760732675097 1106 54-55 0.36462488856486885 1106 56-57 0.47550752782503736 1106 58-59 0.5019286585269214 1106 60-61 0.5290586879869537 1106 62-63 0.6154152314227517 1106 64-65 0.7311558049396112 1106 66-67 0.8159249315952906 1106 68-69 0.7125142746194939 1106 70-71 0.5247488421426141 1106 72-73 0.7295160232574283 1106 74-75 0.6739277362061955 1106 76-77 0.6239180360900924 1106 78-79 0.7744598944218275 1106 80-81 0.744911156407916 1106 82-83 0.5445441647746634 1106 84-85 0.7340552143692669 1106 86-87 0.8999033990820635 1106 88-89 1.17572841618448 1106 90-91 1.034425680992424 1106 92-93 0.9395999879609356 1106 94-95 1.1329336262250251 1106 96-97 1.437576073901642 1106 98-99 1.503631415978564 1106 100-101 1.4249249709278793 1107 1 0.17429767774846283 1107 2 0.20478740054354105 1107 3 0.22685506704395664 1107 4 0.18963556895211298 1107 5 0.1497894909786197 1107 6 0.1871631528876989 1107 7 0.22814542130265636 1107 8 0.2153216015224899 1107 9 0.21496744446987037 1107 10-11 0.3169135621705905 1107 12-13 0.3342397589467154 1107 14-15 0.308492871619066 1107 16-17 0.3826362015325273 1107 18-19 0.4127542004057432 1107 20-21 0.4686898902391121 1107 22-23 0.3678860904716714 1107 24-25 0.4165285355826285 1107 26-27 0.36821448221112973 1107 28-29 0.32258053681413656 1107 30-31 0.374372707311295 1107 32-33 0.40726482708569733 1107 34-35 0.5015930893940137 1107 36-37 0.5582419515939492 1107 38-39 0.5970469919765193 1107 40-41 0.6509511976124074 1107 42-43 0.5868177503750331 1107 44-45 0.6000076181618468 1107 46-47 0.7207471966409642 1107 48-49 0.8362253077156794 1107 50-51 0.6600665133559787 1107 52-53 0.6003372275684384 1107 54-55 0.6901934971871242 1107 56-57 0.9601468807744737 1107 58-59 0.8680715123508946 1107 60-61 1.0324356573325986 1107 62-63 0.9995353995355316 1107 64-65 0.9582264319993996 1107 66-67 0.9603275703331349 1107 68-69 0.9520182888154878 1107 70-71 1.0717263301199296 1107 72-73 1.044965893101672 1107 74-75 0.9927213865081583 1107 76-77 1.1236453340610382 1107 78-79 1.053882949857229 1107 80-81 1.101466293660561 1107 82-83 0.9727214753020696 1107 84-85 0.8423588528670471 1107 86-87 1.0740336013019594 1107 88-89 1.1353784725502756 1107 90-91 1.2396865069310898 1107 92-93 1.3205168752415162 1107 94-95 1.5241725342221066 1107 96-97 1.7637799731534507 1107 98-99 1.7040024124306719 1107 100-101 1.5897219047171554 1108 1 0.030031661989639247 1108 2 0.002901052479135302 1108 3 0.08611498054172628 1108 4 0.07704349975033153 1108 5 0.10856443530887105 1108 6 0.1086222356215103 1108 7 0.09311094888059301 1108 8 0.06336994855914213 1108 9 0.14696363323314898 1108 10-11 0.056409108590592894 1108 12-13 0.233274638186181 1108 14-15 0.199092177888339 1108 16-17 0.25663146960378924 1108 18-19 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-0.5509812000907317 2316 60-61 -0.6653579262468625 2316 62-63 -0.7442185644157959 2316 64-65 -0.7660251530578392 2316 66-67 -0.7456215487539026 2316 68-69 -0.7899567375061949 2316 70-71 -0.9335960921866189 2316 72-73 -0.9548263592180923 2316 74-75 -1.000718862747192 2316 76-77 -1.1503034731428414 2316 78-79 -0.9832628063338618 2316 80-81 -0.9365612127602532 2316 82-83 -0.9886921403867 2316 84-85 -1.1689115896093476 2316 86-87 -1.2188600151403541 2316 88-89 -1.5511695250132718 2316 90-91 -1.3829890215434268 2316 92-93 -1.3781825300872903 2316 94-95 -1.2202810736800522 2316 96-97 -1.3630219894080078 2316 98-99 -1.7034093654499038 2316 100-101 -1.780762330530667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6781.0 3 1228.0 4 1168.0 5 1161.0 6 1354.0 7 1731.0 8 2169.0 9 2707.0 10 3721.0 11 4968.0 12 7357.0 13 10134.0 14 11538.0 15 15405.0 16 20680.0 17 28601.0 18 23739.0 19 14289.0 20 13216.0 21 13808.0 22 14498.0 23 16197.0 24 18160.0 25 20460.0 26 24076.0 27 28073.0 28 33122.0 29 39409.0 30 48212.0 31 59723.0 32 75919.0 33 98546.0 34 131730.0 35 185700.0 36 261817.0 37 355765.0 38 270584.0 39 27997.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06943870486064 19.87298778716512 15.17902827508589 20.878545232888353 2 14.626362925156213 21.101437653629098 41.77098149653718 22.50121792467751 3 18.754059721008566 26.731054458975397 31.09328418999177 23.42160163002427 4 11.598250614125845 17.31739674116579 39.24444007168504 31.83991257302332 5 12.13077272628685 39.47706568102022 34.275812780076315 14.116348812616614 6 29.11993852206668 38.07361390568275 18.146877928354318 14.659569643896258 7 26.613641877075437 35.47134330582732 20.80165067801367 17.113364139083576 8 25.9477661007131 35.09445949002869 20.016828503595086 18.940945905663128 9 26.90055605147955 16.843957293808806 21.16769225158832 35.08779440312332 10-11 24.13067705667153 28.066174175643972 27.294165030565082 20.508983737119415 12-13 25.619116425281113 25.828694826787984 28.24723645073388 20.304952297197023 14-15 22.51512003672094 26.59448440721026 27.87786746901671 23.01252808705209 16-17 20.20976365317617 30.390174559913614 28.234609496420827 21.165452290489387 18-19 20.113332114253822 28.063439347447172 32.114359523459704 19.708869014839298 20-21 21.575179855259684 26.738909112816224 32.09169546561552 19.594215566308574 22-23 20.939063425636494 27.07135152902928 31.837958356035234 20.151626689298986 24-25 20.435461934800937 27.13526251301051 32.39247223037695 20.036803321811597 26-27 20.35004314898263 27.33198716258315 32.20182414768208 20.11614554075214 28-29 20.0293598056069 27.579745169572035 32.365684174641956 20.025210850179107 30-31 20.251031937674902 27.51858465641675 32.12198204974179 20.108401356166556 32-33 20.0484009119901 27.520678215334637 32.00602143922017 20.42489943345509 34-35 20.558633675795317 27.48256747381983 31.84603085461333 20.11276799577153 36-37 20.53134078337757 27.24015890022234 31.958866666754737 20.269633649645353 38-39 20.376005520588073 27.653005042926164 31.875161158055366 20.095828278430396 40-41 20.442864933302616 27.708927367382007 31.820682213537317 20.027525485778057 42-43 20.287169790281336 27.769813904315317 31.417027198925286 20.52598910647806 44-45 20.480249875802002 27.977395964357815 30.99892185567664 20.543432304163538 46-47 20.640374354160855 28.13432820044479 30.39727563696018 20.828021808434173 48-49 20.76279606626134 28.389858277171292 30.09189575423435 20.755449902333016 50-51 20.791130743991744 28.653179807871194 29.882128722918466 20.6735607252186 52-53 20.908087928214446 28.881326937451963 29.58690740386354 20.623677730470057 54-55 20.687051119142005 28.751072452187852 29.65839276574685 20.90348366292329 56-57 20.87840581521209 29.119044719211374 29.275394328350558 20.727155137225974 58-59 21.036842630866186 28.66506953797575 29.45653592509206 20.84155190606601 60-61 20.91746429937591 28.68582394102766 29.806164156298674 20.59054760329775 62-63 20.901727482308008 28.628317859280973 29.91657168763603 20.55338297077499 64-65 20.807098403804936 28.699676555120497 29.843212584629196 20.65001245644537 66-67 20.70229489210298 28.96821790537863 29.470585500238293 20.858901702280097 68-69 20.801399251759634 28.904086787427868 29.51499122825558 20.77952273255691 70-71 20.80686652644972 28.892783706160518 29.387329802458968 20.913019964930793 72-73 20.821417522865808 28.847678494107505 29.30609100566226 21.02481297736443 74-75 20.663270360860352 29.223535590803184 29.151410546583662 20.961783501752798 76-77 20.755171117358717 28.96908967884897 29.23765567382323 21.038083529969086 78-79 20.813917973031103 29.064939690404046 29.05801727117177 21.06312506539308 80-81 20.691756854757216 29.321597983696794 28.959234058404633 21.027411103141358 82-83 20.82407792926934 29.224347824249126 28.858552416056742 21.093021830424792 84-85 20.807085542920454 29.00463834978515 28.86214342586962 21.32613268142478 86-87 20.80721632490897 28.9721275341247 29.06191685809747 21.15873928286886 88-89 20.949289574294067 28.85492859814822 28.850648925220906 21.345132902336807 90-91 20.633258292671265 28.96472683781151 29.115028325846442 21.286986543670785 92-93 20.81834805575765 28.995620521257827 29.124537497140313 21.061493925844207 94-95 20.60734999859981 28.93608207549513 28.972460621610814 21.484107304294252 96-97 20.684153796568797 28.827187564394517 28.90955971320332 21.579098925833364 98-99 20.57261456610858 28.939621344477295 28.75767245977614 21.730091629637986 100-101 20.86633172359563 28.275210470805355 29.47803662890655 21.38042117669246 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3043.0 1 1806.5 2 673.5 3 784.5 4 717.5 5 771.0 6 1405.0 7 2855.5 8 4535.5 9 5257.0 10 4710.5 11 4192.0 12 4353.0 13 4592.0 14 4770.5 15 4844.0 16 5031.0 17 5241.0 18 5408.5 19 5780.5 20 6347.5 21 7155.0 22 8220.5 23 9702.5 24 11399.0 25 13556.0 26 16776.0 27 20412.0 28 23213.5 29 25685.0 30 29134.0 31 33151.5 32 37423.5 33 42436.0 34 47581.5 35 51577.5 36 55982.0 37 60576.0 38 62583.0 39 63185.5 40 64452.0 41 65961.5 42 67199.0 43 68508.0 44 69878.0 45 70547.5 46 69317.0 47 70029.0 48 78238.0 49 75990.0 50 63922.0 51 57680.0 52 51992.0 53 47478.0 54 44072.5 55 39428.5 56 34657.0 57 30146.5 58 26733.5 59 23561.0 60 19560.5 61 15984.5 62 12666.0 63 9058.0 64 6023.5 65 4432.5 66 3210.0 67 2350.0 68 1770.5 69 1254.5 70 912.5 71 722.5 72 573.0 73 524.0 74 430.5 75 326.0 76 247.0 77 168.0 78 118.0 79 80.0 80 57.5 81 39.0 82 28.5 83 24.5 84 15.0 85 9.5 86 7.0 87 5.5 88 3.0 89 2.0 90 1.0 91 0.0 92 0.0 93 1.0 94 2.0 95 2.0 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.308795828144097 2 0.27640760838316847 3 0.27572186431656903 4 0.27740984971127536 5 0.2857970271412226 6 0.19464581582707857 7 0.1941183203912328 8 0.1963865507653695 9 0.19306332951954133 10-11 0.2006856385675123 12-13 0.1909005982325738 14-15 0.19214021250681126 16-17 0.19857565682412934 18-19 0.19058410097106634 20-21 0.18280354329234166 22-23 0.18314641532564138 24-25 0.18623226362533898 26-27 0.18304091623847224 28-29 0.19562168238339317 30-31 0.200474640393174 32-33 0.20279562031089524 34-35 0.20116038445977344 36-37 0.17768683756463796 38-39 0.16874578992705266 40-41 0.17528673333153985 42-43 0.18596851590741612 44-45 0.1904786018838972 46-47 0.19195558910426527 48-49 0.1902676037095589 50-51 0.19448756719632482 52-53 0.21716987093769172 54-55 0.1833310387281874 56-57 0.16866666561167581 58-59 0.16816554494762234 60-61 0.16178285017388888 62-63 0.17027552669100532 64-65 0.16650393432470825 66-67 0.16420932917877928 68-69 0.17465373880852494 70-71 0.16919416104752152 72-73 0.16523794527867847 74-75 0.1690359124167678 76-77 0.17367787225221035 78-79 0.17665822146473878 80-81 0.16766442428356887 82-83 0.16684680635800797 84-85 0.16099160702012025 86-87 0.15756288668712295 88-89 0.16262684287124204 90-91 0.16993265465770557 92-93 0.16141360336879687 94-95 0.16626656137857768 96-97 0.16463132552745588 98-99 0.1680864206322455 100-101 0.1473031004599233 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1895751.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.10740667086827 #Duplication Level Percentage of deduplicated Percentage of total 1 70.83182506038722 34.78367238480233 2 14.937469552605517 14.670807839070235 3 5.771155390829378 8.50219424214696 4 2.833430027079217 5.565696024529136 5 1.6453099528286712 4.0398452476592315 6 0.9717163557704378 2.86310821509318 7 0.6334159714037793 2.1773790989683914 8 0.4567276309838858 1.7942967610038347 9 0.3148889809597764 1.3917043119750332 >10 1.4489176852060524 12.5343162174115 >50 0.10118308673999878 3.4403379305802035 >100 0.04934824705236172 4.305683767746079 >500 0.003110457824634108 1.0167650932683832 >1k 0.001179828830033627 1.0504219982931706 >5k 1.0725716636669335E-4 0.4333787395054008 >10k+ 2.145143327333867E-4 1.4303921279470306 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15177 0.8005798229830816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11980 0.6319395321431981 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8228 0.434023244613876 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2671 0.1408940309143975 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2470 0.13029137265389812 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2444 0.1289198845206992 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2264 0.11942496667547585 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2007 0.1058683339742403 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1938 0.10222861546690468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.2749543584574135E-5 0.0 0.0 0.0 0.0 6 5.2749543584574135E-5 0.0 0.0 0.0 0.0 7 5.2749543584574135E-5 0.0 0.0 0.0 0.0 8 5.2749543584574135E-5 0.0 0.0 0.0 0.0 9 5.2749543584574135E-5 0.0 0.0 0.0 0.0 10-11 5.2749543584574135E-5 0.0 0.0 0.0 0.0 12-13 1.0549908716914827E-4 0.0 0.0 0.0 0.0 14-15 1.0549908716914827E-4 0.0 0.0 0.0 0.0 16-17 1.3187385896143533E-4 0.0 0.0 5.2749543584574135E-5 0.0 18-19 1.8462340254600946E-4 0.0 0.0 1.0549908716914827E-4 0.0 20-21 2.1099817433829654E-4 0.0 0.0 1.0549908716914827E-4 0.0 22-23 2.1099817433829654E-4 0.0 0.0 2.901224897151577E-4 0.0 24-25 2.1099817433829654E-4 0.0 0.0 4.219963486765931E-4 0.0 26-27 2.1099817433829654E-4 0.0 0.0 6.593692948071766E-4 0.0 28-29 2.6374771792287067E-4 0.0 0.0 0.001239614274237492 0.0 30-31 3.164972615074448E-4 0.0 0.0 0.0028484753535670034 5.2749543584574135E-5 32-33 3.164972615074448E-4 0.0 0.0 0.004879332781573107 5.2749543584574135E-5 34-35 3.692468050920189E-4 0.0 0.0 0.007042064068540647 5.2749543584574135E-5 36-37 4.219963486765931E-4 0.0 0.0 0.009864164650315362 5.2749543584574135E-5 38-39 4.219963486765931E-4 0.0 0.0 0.014980870378019054 5.2749543584574135E-5 40-41 4.4837112046888013E-4 0.0 0.0 0.025029658430880425 5.2749543584574135E-5 42-43 5.274954358457413E-4 0.0 0.0 0.03410257992742718 5.2749543584574135E-5 44-45 5.802449794303154E-4 0.0 0.0 0.04496898590584945 5.2749543584574135E-5 46-47 6.593692948071766E-4 0.0 0.0 0.06395882159629614 5.2749543584574135E-5 48-49 6.857440665994637E-4 0.0 0.0 0.07878144334356146 5.2749543584574135E-5 50-51 6.857440665994637E-4 0.0 0.0 0.0970591601956164 5.2749543584574135E-5 52-53 7.121188383917509E-4 0.0 0.0 0.11916121895755297 5.2749543584574135E-5 54-55 7.64868381976325E-4 0.0 0.0 0.1504680730749977 5.2749543584574135E-5 56-57 7.912431537686121E-4 0.0 0.0 0.21954360039899756 5.2749543584574135E-5 58-59 7.912431537686121E-4 0.0 0.0 0.27561636522939986 5.2749543584574135E-5 60-61 8.439926973531862E-4 0.0 0.0 0.3235129508041932 5.2749543584574135E-5 62-63 8.439926973531862E-4 0.0 0.0 0.37977033903714147 5.2749543584574135E-5 64-65 8.439926973531862E-4 0.0 0.0 0.4348144877676446 5.2749543584574135E-5 66-67 8.439926973531862E-4 0.0 0.0 0.5250953316126432 5.2749543584574135E-5 68-69 8.967422409377603E-4 0.0 0.0 0.6373991099042016 5.2749543584574135E-5 70-71 9.231170127300474E-4 0.0 0.0 0.706712010174332 5.2749543584574135E-5 72-73 9.494917845223345E-4 0.0 0.0 0.7847549599077095 5.2749543584574135E-5 74-75 9.494917845223345E-4 0.0 0.0 0.9156793270846224 5.2749543584574135E-5 76-77 9.494917845223345E-4 0.0 0.0 1.0670441424005577 5.2749543584574135E-5 78-79 9.494917845223345E-4 0.0 0.0 1.2233674148134432 5.2749543584574135E-5 80-81 0.0010022413281069085 0.0 0.0 1.3764202155240852 5.2749543584574135E-5 82-83 0.0010549908716914827 0.0 0.0 1.5762882361660364 5.2749543584574135E-5 84-85 0.0010549908716914827 0.0 0.0 1.8575751773307783 5.2749543584574135E-5 86-87 0.0010549908716914827 0.0 0.0 2.08801155847999 5.2749543584574135E-5 88-89 0.0010549908716914827 0.0 0.0 2.401765843721037 5.2749543584574135E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11410 0.0 32.21865 1 GTATCAA 16570 0.0 29.819921 1 GTACATG 13180 0.0 25.185669 1 TACATGG 13345 0.0 24.510122 2 ATCAACG 20355 0.0 24.151077 3 TATCAAC 20535 0.0 24.008204 2 TCAACGC 20560 0.0 23.910904 4 CAACGCA 20590 0.0 23.876066 5 AACGCAG 21155 0.0 23.329168 6 GAGTACT 10685 0.0 23.026863 12-13 ACATGGG 13470 0.0 22.836197 3 AGTACTT 11120 0.0 22.190153 12-13 CATGGGA 9875 0.0 21.088009 4 GTACTTT 11715 0.0 21.002031 14-15 ACGCAGA 23540 0.0 20.884815 7 CGCAGAG 23605 0.0 20.806631 8 GCAGAGT 24005 0.0 19.74759 9 GTGTAGC 995 0.0 19.596306 1 ATGGGCG 320 3.929017E-10 19.314877 5 GACCGAC 150 7.7232014E-4 19.00014 7 >>END_MODULE