FastQCFastQC Report
Thu 26 May 2016
SRR937587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895751
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201051.0605295737678628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174660.9213235282481719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121880.6429114372087896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35280.18610038976637755No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.16616106229140853No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26290.13867855008384541No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21080.11119603787628227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136750.033.9872061
GTATCAA200350.029.8160321
GAGTACT152800.024.18480112-13
ATCAACG248850.023.9069843
TATAGCG1000.001654203923.7495967
TATCAAC251000.023.7022022
TCAACGC252150.023.59414
CAACGCA252350.023.5754055
AACGCAG257100.023.2593966
GTACATG126500.023.1792091
AGTACTT156950.023.0308312-13
TACATGG126000.022.8538932
GTACTTT163300.022.42554514-15
ACATGGG126600.021.694633
ATACGTC2457.494236E-921.3346085
ACGCAGA284350.020.9307167
CGCAGAG284600.020.8956418
GTATAGA8000.020.1972791
GCAGAGT291900.020.1289849
TACTTTT180600.019.85655814-15
Produced by FastQC (version 0.11.5)