##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937587_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1895751 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17503406301777 33.0 31.0 34.0 31.0 34.0 2 32.44179958232911 34.0 31.0 34.0 31.0 34.0 3 32.50922352144348 34.0 31.0 34.0 31.0 34.0 4 36.151094210157346 37.0 35.0 37.0 35.0 37.0 5 36.07885634769545 37.0 35.0 37.0 35.0 37.0 6 36.13526459962305 37.0 35.0 37.0 35.0 37.0 7 36.078577830105324 37.0 35.0 37.0 35.0 37.0 8 36.064957370456355 37.0 35.0 37.0 35.0 37.0 9 37.75083278341934 39.0 37.0 39.0 35.0 39.0 10-11 37.67423701741421 39.0 37.0 39.0 35.0 39.0 12-13 37.57205376655479 39.0 37.0 39.0 35.0 39.0 14-15 38.73324872306543 40.0 38.0 41.0 34.5 41.0 16-17 38.70789359995062 40.0 38.0 41.0 34.5 41.0 18-19 38.76836172050021 40.0 38.0 41.0 35.0 41.0 20-21 38.72122261837129 40.0 38.0 41.0 34.5 41.0 22-23 38.66655615637286 40.0 38.0 41.0 34.5 41.0 24-25 38.67644564080409 40.0 38.0 41.0 34.0 41.0 26-27 38.53780216916673 40.0 38.0 41.0 34.0 41.0 28-29 38.385937815673046 40.0 38.0 41.0 34.0 41.0 30-31 38.33398505394432 40.0 38.0 41.0 34.0 41.0 32-33 38.17346661033015 40.0 37.0 41.0 34.0 41.0 34-35 37.9983164983165 40.0 37.0 41.0 33.5 41.0 36-37 37.821973455374675 40.0 37.0 41.0 33.0 41.0 38-39 37.66722871305356 40.0 37.0 41.0 33.0 41.0 40-41 37.45334803990608 40.0 36.5 41.0 33.0 41.0 42-43 37.173472149032236 40.0 36.0 41.0 32.5 41.0 44-45 36.76264657120055 40.0 36.0 41.0 31.5 41.0 46-47 36.215753018197006 40.0 36.0 41.0 30.0 41.0 48-49 35.74479586190381 39.0 35.0 41.0 28.5 41.0 50-51 35.4372581103742 39.0 35.0 41.0 27.0 41.0 52-53 35.198857603134584 39.0 35.0 40.5 26.0 41.0 54-55 34.97923303218619 39.0 35.0 40.0 25.5 41.0 56-57 34.73115825865317 38.5 35.0 40.0 24.5 41.0 58-59 34.49011631801856 38.0 34.0 40.0 24.0 41.0 60-61 34.181402779162454 38.0 34.0 40.0 23.0 41.0 62-63 33.83246296586419 38.0 34.0 40.0 22.0 41.0 64-65 33.4431164746847 37.0 33.0 40.0 21.5 41.0 66-67 33.03310798728314 36.5 33.0 39.0 19.5 41.0 68-69 32.60414236890816 36.0 32.0 39.0 18.0 40.0 70-71 32.22078479715954 35.5 32.0 39.0 16.0 40.0 72-73 31.740599371963935 35.0 31.0 38.0 12.0 40.0 74-75 31.267811806508345 35.0 31.0 37.0 8.0 39.0 76-77 30.022282462200995 33.5 29.5 36.0 7.0 38.5 78-79 30.600392140107004 34.0 31.0 36.0 7.0 39.0 80-81 30.404423101979113 34.0 31.0 36.0 2.0 38.0 82-83 30.02066568869013 34.0 30.0 35.5 2.0 37.0 84-85 29.63437313233647 34.0 30.0 35.0 2.0 37.0 86-87 29.25721890691341 34.0 29.5 35.0 2.0 36.0 88-89 28.819093066547243 34.0 29.0 35.0 2.0 36.0 90-91 28.277051680310336 34.0 29.0 35.0 2.0 35.5 92-93 27.855319079351666 33.5 27.5 35.0 2.0 35.0 94-95 27.401147619070226 33.0 27.0 35.0 2.0 35.0 96-97 26.844060216768973 33.0 25.5 35.0 2.0 35.0 98-99 25.988934464494548 32.5 24.5 34.5 2.0 35.0 100-101 23.255766975726242 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39002493673532257 1101 2 0.32001522203949406 1101 3 0.23199070769823038 1101 4 0.10901381708486468 1101 5 0.11375980164791599 1101 6 0.13590440704700768 1101 7 0.20759414729084824 1101 8 0.17864252659376234 1101 9 0.286167217430652 1101 10-11 0.276302430864682 1101 12-13 0.3560463236809639 1101 14-15 0.3781612347592471 1101 16-17 0.36495610087882824 1101 18-19 0.3315553771636175 1101 20-21 0.3877821154810448 1101 22-23 0.3110552440735148 1101 24-25 0.19098059628700526 1101 26-27 0.15438766717594632 1101 28-29 0.11202816609357313 1101 30-31 0.1964277011658382 1101 32-33 0.22985154145127495 1101 34-35 0.2162520278097375 1101 36-37 0.25571254962320467 1101 38-39 0.331923166474283 1101 40-41 0.37473077307898706 1101 42-43 0.3933517215601725 1101 44-45 0.4217466020163627 1101 46-47 0.3201067181115249 1101 48-49 0.4385704486307489 1101 50-51 0.3571723782528622 1101 52-53 0.3154337183737397 1101 54-55 0.177133123873638 1101 56-57 0.170237765439758 1101 58-59 0.32598675313601433 1101 60-61 0.3802561960781219 1101 62-63 0.19905987632708388 1101 64-65 0.20244265462405764 1101 66-67 0.21867638318624216 1101 68-69 0.22644641425343792 1101 70-71 0.09513753475823705 1101 72-73 0.033714020257992416 1101 74-75 0.1369766101237211 1101 76-77 0.08276101338755026 1101 78-79 0.14487442319028432 1101 80-81 -0.039456276056423434 1101 82-83 -0.04441921695838147 1101 84-85 -0.08117749585682077 1101 86-87 -0.11279324739978591 1101 88-89 -0.13773561919435906 1101 90-91 -0.07120758113455494 1101 92-93 -0.18221359644851987 1101 94-95 -0.25991806309183474 1101 96-97 -0.26300471598925057 1101 98-99 -0.37358626020707675 1101 100-101 -0.1842736585009277 1102 1 0.318447564269313 1102 2 0.19292459941218 1102 3 0.19539300068787213 1102 4 0.0688350609529067 1102 5 0.06749913258158102 1102 6 0.09202104022345736 1102 7 0.1402989380133377 1102 8 0.11267742127569136 1102 9 0.20036013673767883 1102 10-11 0.26713091957808643 1102 12-13 0.21303051562743747 1102 14-15 0.32557600243553964 1102 16-17 0.18625504048573305 1102 18-19 0.10821392566740684 1102 20-21 0.17067353449342448 1102 22-23 0.24918612093574666 1102 24-25 0.0880870750506304 1102 26-27 0.11999254299377071 1102 28-29 0.08569892874241702 1102 30-31 0.11147325121610407 1102 32-33 0.3091339003255058 1102 34-35 0.1764442359388596 1102 36-37 0.1593051721588168 1102 38-39 0.08746023438617812 1102 40-41 0.14322111906256652 1102 42-43 -0.04354996927648358 1102 44-45 0.12364257323825711 1102 46-47 0.08552899117476187 1102 48-49 0.2595800528870811 1102 50-51 0.22956095199452164 1102 52-53 0.24884856879715045 1102 54-55 0.26997007515684146 1102 56-57 0.3706082265089208 1102 58-59 0.1358984597281605 1102 60-61 -0.038249077932242415 1102 62-63 0.10535016662409191 1102 64-65 0.08352442778290481 1102 66-67 -0.11050030538604716 1102 68-69 0.02622271749253713 1102 70-71 0.05349784476918984 1102 72-73 0.04824480498019312 1102 74-75 0.04038221299795097 1102 76-77 0.018870176959584484 1102 78-79 0.0856109955140596 1102 80-81 0.16897084665866302 1102 82-83 0.048166697469920194 1102 84-85 0.12050266194416537 1102 86-87 -0.0721079447410311 1102 88-89 0.007460407602284391 1102 90-91 -0.161675896257929 1102 92-93 -0.20784004242639398 1102 94-95 -0.25887086480850385 1102 96-97 -0.21761568713846202 1102 98-99 -0.056361218246461675 1102 100-101 -0.18776720813969305 1103 1 0.2796575080420709 1103 2 0.20408174360545672 1103 3 0.18974624110857263 1103 4 0.113942778887683 1103 5 0.09584945008444379 1103 6 0.09844842891457262 1103 7 0.1488845519697719 1103 8 0.1877270204848216 1103 9 0.2188449533969532 1103 10-11 0.14641085976121104 1103 12-13 0.23758597765379363 1103 14-15 0.17965008695980345 1103 16-17 0.21495948876540183 1103 18-19 0.2585439426332883 1103 20-21 0.1251940510880658 1103 22-23 0.07750715178418233 1103 24-25 0.13905173198921972 1103 26-27 0.142159875598729 1103 28-29 0.04384984096552813 1103 30-31 0.14972237953972467 1103 32-33 0.2545327082724569 1103 34-35 0.31494777411156605 1103 36-37 0.17347366891481641 1103 38-39 0.23649259914375165 1103 40-41 0.2626235406848423 1103 42-43 0.23652149434040126 1103 44-45 0.13692202017245592 1103 46-47 0.18196646952328166 1103 48-49 0.07502749601323444 1103 50-51 0.10812387859082406 1103 52-53 0.026627130339093696 1103 54-55 -0.0762721152863648 1103 56-57 0.10200035887519476 1103 58-59 0.08063394193383289 1103 60-61 0.05238149169188233 1103 62-63 -0.006335704877258763 1103 64-65 0.09457275938412835 1103 66-67 0.025123944486331595 1103 68-69 0.07715092939728407 1103 70-71 0.047407997179973904 1103 72-73 0.01242900314503359 1103 74-75 -0.1509007880455009 1103 76-77 0.04443120841413162 1103 78-79 0.08592437055409619 1103 80-81 -0.003795464649325453 1103 82-83 -0.1261445436415407 1103 84-85 1.0616610151004124E-4 1103 86-87 -0.09573895693065282 1103 88-89 -0.02094607966598616 1103 90-91 0.08595151897666753 1103 92-93 -0.07341037977417031 1103 94-95 -0.3013420000804494 1103 96-97 -0.2604538852959912 1103 98-99 0.046269564561512766 1103 100-101 0.05203432187246548 1104 1 0.23960411058330067 1104 2 0.2000885511248356 1104 3 0.14237822013546975 1104 4 0.08721424955784585 1104 5 0.06524819075902144 1104 6 0.11808962243768661 1104 7 0.06113432398757368 1104 8 0.1063408483153978 1104 9 0.13381561181660828 1104 10-11 0.1423230903400352 1104 12-13 0.1895280122171883 1104 14-15 0.20980321068841334 1104 16-17 0.23491749609871704 1104 18-19 0.2011857430145696 1104 20-21 0.16582665338836478 1104 22-23 0.17330500613771704 1104 24-25 0.06945093254258694 1104 26-27 0.10209637646406122 1104 28-29 0.038583963593310955 1104 30-31 0.2400766932962668 1104 32-33 0.2252790125111943 1104 34-35 0.17606425173800488 1104 36-37 0.04148277254513033 1104 38-39 -0.025337915443195413 1104 40-41 -0.09569641424148045 1104 42-43 0.09314348382000759 1104 44-45 -7.263197812577005E-4 1104 46-47 0.08719810221422364 1104 48-49 0.13259189609111388 1104 50-51 0.16721880883748952 1104 52-53 0.10944319473122022 1104 54-55 0.023961511222857723 1104 56-57 0.23955058630179593 1104 58-59 0.2519011643442042 1104 60-61 0.21903111178047396 1104 62-63 0.18779123126677177 1104 64-65 0.19832694621310765 1104 66-67 0.13803870982818722 1104 68-69 0.15817937596914788 1104 70-71 -0.03279842551607004 1104 72-73 0.19023983865717398 1104 74-75 0.2594608544768988 1104 76-77 0.19203083556127254 1104 78-79 0.20242813506989066 1104 80-81 0.201797252446422 1104 82-83 0.11421397493758789 1104 84-85 0.09740487695088973 1104 86-87 0.10687730136313789 1104 88-89 0.03859143025575307 1104 90-91 0.07329146739246184 1104 92-93 0.03846328159593426 1104 94-95 -0.06495230368884464 1104 96-97 0.023487556299023993 1104 98-99 0.3580689028512367 1104 100-101 -0.11308653584062967 1105 1 0.27995129568361676 1105 2 0.17817816429268873 1105 3 0.15424065028808087 1105 4 0.0933419623247218 1105 5 0.04344742357479703 1105 6 0.1026421954075758 1105 7 0.12938648086274185 1105 8 0.10238457556739888 1105 9 0.20461917238542782 1105 10-11 0.16699044964615695 1105 12-13 0.27300171321158473 1105 14-15 0.17427635157496013 1105 16-17 0.2664603581476257 1105 18-19 0.3093362576903118 1105 20-21 0.07246776428256396 1105 22-23 0.1337047166805263 1105 24-25 0.22640237691559406 1105 26-27 0.27790252804273763 1105 28-29 0.15100185106683028 1105 30-31 0.2228135151748134 1105 32-33 0.14572644976225746 1105 34-35 0.2535337856526283 1105 36-37 0.205657870640664 1105 38-39 0.2902846474999521 1105 40-41 0.3642445035790516 1105 42-43 0.3773348485514063 1105 44-45 0.3651022974371543 1105 46-47 0.23951485853562104 1105 48-49 0.34236135753924657 1105 50-51 0.35215149879633145 1105 52-53 0.41112834768256334 1105 54-55 0.3050164508528397 1105 56-57 0.3722534746389812 1105 58-59 0.37850700275308924 1105 60-61 0.2545675530309879 1105 62-63 0.21345756030152074 1105 64-65 0.39156198300339895 1105 66-67 0.35987412602001 1105 68-69 0.4364184449227153 1105 70-71 0.1822237079429101 1105 72-73 0.2844542209594003 1105 74-75 0.06679103601112502 1105 76-77 0.018140600346637115 1105 78-79 0.2156419020711553 1105 80-81 -0.03457362685135834 1105 82-83 0.00267521659857195 1105 84-85 0.2251226265438362 1105 86-87 0.24778760949400436 1105 88-89 0.23299791524169322 1105 90-91 0.4256451724343542 1105 92-93 0.42397092533054703 1105 94-95 0.06676349676966353 1105 96-97 -0.03383930342768693 1105 98-99 -0.11299380980832652 1105 100-101 -0.14037301260892932 1106 1 0.08533143771883545 1106 2 0.14618874288838413 1106 3 0.09652696199195532 1106 4 0.07671869162452083 1106 5 0.028714529800609512 1106 6 -0.040734688614676884 1106 7 -0.010075372314481967 1106 8 -0.03602737519548782 1106 9 -0.006395740649502102 1106 10-11 -0.062238888826904315 1106 12-13 -0.15189655677027503 1106 14-15 0.06548131905008603 1106 16-17 0.07061487725916749 1106 18-19 0.08129883474763488 1106 20-21 0.05644735860027339 1106 22-23 0.22841586089732857 1106 24-25 0.05787452797776638 1106 26-27 0.08688107245803423 1106 28-29 0.0720060882519391 1106 30-31 0.022952433841631148 1106 32-33 -0.07517826772558323 1106 34-35 -0.41149236244328335 1106 36-37 -0.002767375202274991 1106 38-39 -0.029562044644343644 1106 40-41 -0.18414688014812697 1106 42-43 -0.12905629425079468 1106 44-45 6.126494749523204E-4 1106 46-47 -0.059520833725819955 1106 48-49 -0.08197631981137476 1106 50-51 0.10669260302842787 1106 52-53 0.0566019973422911 1106 54-55 -0.0897095577718332 1106 56-57 -0.004994145398015348 1106 58-59 0.08561848912756886 1106 60-61 0.015198091266228175 1106 62-63 -0.0874942038660862 1106 64-65 -0.23821949362356776 1106 66-67 -0.33523200253667795 1106 68-69 0.05966842337208078 1106 70-71 0.05047753235059815 1106 72-73 0.045874948906476476 1106 74-75 0.04295410685411838 1106 76-77 -0.04980742616836764 1106 78-79 -0.25330336596340786 1106 80-81 -0.25133868963552075 1106 82-83 -0.2620358502570035 1106 84-85 -0.5453722974542785 1106 86-87 -0.24497745771080304 1106 88-89 -0.3406875516775365 1106 90-91 -0.5103950487745621 1106 92-93 -0.5615799308372438 1106 94-95 -0.5770626349698809 1106 96-97 -0.25903093824731727 1106 98-99 -0.26115990658059474 1106 100-101 -0.5728772730024119 1107 1 0.15555248677532774 1107 2 0.0996645598051984 1107 3 0.05059520055810651 1107 4 -0.0030253556718591312 1107 5 -0.042248290900850805 1107 6 0.028365682326459307 1107 7 0.0879668151872437 1107 8 0.09844293568482243 1107 9 0.08124283678625943 1107 10-11 0.09127492936657688 1107 12-13 0.1700616539946509 1107 14-15 0.09142673217983344 1107 16-17 0.16852292633889476 1107 18-19 0.15241957924076388 1107 20-21 0.20045947475696124 1107 22-23 0.08210569082640262 1107 24-25 0.016637334739890264 1107 26-27 0.0608485384975026 1107 28-29 0.12482537365092838 1107 30-31 0.04274794604021537 1107 32-33 0.0861747657940839 1107 34-35 0.02944884122917557 1107 36-37 0.034020847610214844 1107 38-39 -0.011425700267601258 1107 40-41 0.03592531674605226 1107 42-43 0.10988738088752115 1107 44-45 -0.09420136552863312 1107 46-47 -0.3420268602747214 1107 48-49 -0.4389441055704708 1107 50-51 -0.3230918793963937 1107 52-53 -0.3167146346051126 1107 54-55 -0.2887759543546977 1107 56-57 -0.4432133071130835 1107 58-59 -0.49990097781464016 1107 60-61 -0.44235774627195923 1107 62-63 -0.500304782713485 1107 64-65 -0.5050993620185622 1107 66-67 -0.2803223703469939 1107 68-69 -0.41277650243415565 1107 70-71 -0.44358746476749644 1107 72-73 -0.3867034847328945 1107 74-75 -0.4686296447513918 1107 76-77 -0.5288931718728982 1107 78-79 -0.5078969846252974 1107 80-81 -0.3153575652623779 1107 82-83 -0.4351725984318868 1107 84-85 -0.4505663030986007 1107 86-87 -0.4924893974132303 1107 88-89 -0.5067235372824079 1107 90-91 -0.5688011303063867 1107 92-93 -0.6559326202987741 1107 94-95 -0.6093039801376321 1107 96-97 -0.43156005319923807 1107 98-99 -0.19538838109761514 1107 100-101 -0.13104439454122385 1108 1 0.06582569677875227 1108 2 0.04014449728379077 1108 3 -0.007070095776256835 1108 4 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60-61 -0.3061589950605281 2316 62-63 -0.1580535618688046 2316 64-65 -0.22874157132356032 2316 66-67 -0.37582900257394414 2316 68-69 -0.2956248644195796 2316 70-71 -0.39408569289433615 2316 72-73 -0.3323567962413847 2316 74-75 -0.23288861871856525 2316 76-77 -0.17039210539991956 2316 78-79 -0.33932073128406515 2316 80-81 -0.2841946282858032 2316 82-83 -0.37771440438195114 2316 84-85 -0.2852514790195464 2316 86-87 -0.3058618591761437 2316 88-89 -0.39302877880347964 2316 90-91 -0.2968395317063006 2316 92-93 -0.15701767021054636 2316 94-95 -0.19748371275537835 2316 96-97 -0.42171270849717146 2316 98-99 -0.3865586337229807 2316 100-101 -0.1835135045020202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 15.0 5 43.0 6 72.0 7 181.0 8 497.0 9 911.0 10 1702.0 11 3032.0 12 4994.0 13 7405.0 14 9739.0 15 14186.0 16 20831.0 17 31274.0 18 27517.0 19 13878.0 20 10130.0 21 9591.0 22 9812.0 23 10195.0 24 11945.0 25 13860.0 26 16871.0 27 20472.0 28 25587.0 29 31859.0 30 39885.0 31 50427.0 32 64628.0 33 85698.0 34 119427.0 35 175396.0 36 266987.0 37 417770.0 38 348411.0 39 30515.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35566270323429 20.296706096988988 15.969728992814753 20.377902206961974 2 15.179314160984223 21.57419407928573 40.92868736453258 22.31780439519747 3 18.839130244425558 26.587088705214978 31.782338503316097 22.79144254704336 4 11.89930106817882 17.974152710009232 38.73052881445338 31.396017407358567 5 12.054132094468379 39.413150346056185 34.32678932893173 14.205928230543707 6 29.066000756428455 38.43041623082356 17.610118628448568 14.893464384299415 7 26.218158397384467 35.853812024891454 20.637467684310863 17.290561893413216 8 26.306039136996368 35.09748906897583 19.626153434707405 18.970318359320395 9 26.707252165500638 16.53951389185605 21.108679357151864 35.64455458549145 10-11 24.218950695529106 28.135736180542697 26.971659252718318 20.67365387120988 12-13 25.88315414493081 25.942341443188898 28.196048166433417 19.97845624544688 14-15 22.60805875877159 26.445983676126243 28.23854504098903 22.707412524113135 16-17 19.958264561115886 30.120305884053337 28.779306987046294 21.142122567784483 18-19 19.855824947474645 27.333178249675193 33.241048006832116 19.569948796018043 20-21 21.143203933427966 26.03437898753581 33.55767714219853 19.264739936837696 22-23 20.567978581456693 26.299089986208557 33.33512682774884 19.797804604585913 24-25 20.18045675974353 26.38406009247024 33.868347272444346 19.567135875341883 26-27 20.150459308914755 26.54496675024931 33.725873720841356 19.578700219994584 28-29 19.82366677937617 26.742166206245727 33.89123656387596 19.542930450502155 30-31 20.042663830851204 26.666766890799476 33.61369715748535 19.676872120863976 32-33 19.88267446515919 26.63611940597684 33.48978847960518 19.99141764925879 34-35 20.3648052935222 26.524132124946785 33.39109408355844 19.71996849797257 36-37 20.302824579810324 26.343148440908116 33.458138753454435 19.895888225827125 38-39 20.15087951951496 26.735921542438856 33.41040041651039 19.70279852153579 40-41 20.22365965752115 26.86832960787029 33.27362042476872 19.634390309839837 42-43 20.129200765782663 26.904314622935395 32.860601283936234 20.105883327345712 44-45 20.324082645874906 27.071883385529006 32.38605702964155 20.217976938954536 46-47 20.47724094567272 27.41372680272884 31.675045931665075 20.433986319933368 48-49 20.637784181572368 27.660383668530308 31.221821853186416 20.48001029671091 50-51 20.709840058108895 27.948158803555952 30.905614714168685 20.436386424166464 52-53 20.87919246778717 28.253605035682426 30.39840147783121 20.468801018699185 54-55 20.58422787814849 28.13356191480944 30.516365554529866 20.765844652512197 56-57 20.851314334002723 28.42190245448901 30.09783457848631 20.628948633021952 58-59 21.013677429156044 28.001910588468633 30.32821821009194 20.656193772283384 60-61 20.878744096666704 28.00278095593778 30.728191624321973 20.390283323073547 62-63 20.930755146641093 27.88530772237493 30.810823784347207 20.373113346636767 64-65 20.799909903779557 28.004838188137576 30.663731682061623 20.531520226021243 66-67 20.790889731826596 28.203361095418124 30.35857557242486 20.647173600330422 68-69 20.927458300167057 28.10522056957902 30.308015134899048 20.659305995354877 70-71 20.910710320078955 28.15285340743589 30.250781880109784 20.68565439237537 72-73 20.875658248367007 28.16332419183743 30.091873880061254 20.86914367973431 74-75 20.746685614302724 28.520860598253673 29.811879302714335 20.920574484729272 76-77 20.75620690691974 28.22836437907721 30.15825918066244 20.857169533340613 78-79 20.76891954692362 28.32373555387812 29.922758843329106 20.98458605586915 80-81 20.668405291623216 28.526109177840336 29.89846767850841 20.907017852028034 82-83 20.81747314493821 28.506112678374368 29.665849392213666 21.010564784473754 84-85 20.703948491084926 28.283457281671442 29.710781936115506 21.301812291128126 86-87 20.682700417934637 28.264181319171133 29.847986365297974 21.205131897596257 88-89 20.746606489987347 28.089685828993368 29.737054075139614 21.426653605879675 90-91 20.536254087230116 28.093237909237377 29.96687483975526 21.40363316377725 92-93 20.553266484607718 28.168171193787277 30.01129384409009 21.26726847751491 94-95 20.322341910936615 28.08248551629407 29.9771436227648 21.61802895000451 96-97 20.401914597433944 27.92463250711723 29.9351022365279 21.738350658920925 98-99 20.25865559122352 28.06244891467673 29.6925108619365 21.986384632163254 100-101 20.22730222443107 27.764711096197857 30.23283665874224 21.775150020628825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1114.0 1 804.5 2 695.0 3 953.5 4 777.5 5 683.5 6 1526.5 7 3813.5 8 6134.5 9 7050.5 10 6059.0 11 4676.5 12 4681.5 13 4975.0 14 5219.0 15 5480.5 16 5643.5 17 5908.0 18 6134.5 19 6373.5 20 6959.0 21 7744.0 22 8775.5 23 10266.5 24 11784.0 25 13746.0 26 16939.0 27 20545.5 28 23274.5 29 25723.5 30 28891.0 31 32591.5 32 36919.5 33 41606.5 34 46485.5 35 50645.0 36 55079.0 37 59491.5 38 61930.5 39 63087.5 40 64146.0 41 65547.0 42 66969.5 43 68685.5 44 70367.0 45 70931.0 46 69247.5 47 69853.0 48 78915.5 49 76270.0 50 63564.5 51 57589.5 52 51761.5 53 47730.5 54 43948.0 55 38804.0 56 33907.5 57 29203.5 58 26147.0 59 23506.0 60 19802.0 61 15725.5 62 11997.5 63 8579.5 64 5877.0 65 4289.0 66 3000.0 67 2210.0 68 1612.5 69 1033.5 70 765.0 71 638.0 72 526.0 73 474.5 74 393.0 75 337.5 76 261.5 77 159.0 78 107.5 79 81.5 80 55.5 81 33.5 82 22.5 83 19.0 84 10.5 85 5.5 86 4.5 87 2.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017934844818755204 2 0.0 3 0.0 4 5.274954358457413E-5 5 0.036027938268264136 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0039034662252584863 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.538702076380284E-4 24-25 3.1649726150744477E-4 26-27 0.001186864730652918 28-29 0.0011604899588606309 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.121188383917509E-4 42-43 0.0091784205837159 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.274954358457413E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010022413281069087 84-85 7.912431537686119E-5 86-87 0.0 88-89 0.0 90-91 0.01123565278351429 92-93 0.0014769872203680757 94-95 0.0 96-97 0.0 98-99 0.0013451133614066405 100-101 0.018198592536678077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1895751.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.87106904279783 #Duplication Level Percentage of deduplicated Percentage of total 1 69.26690337935682 32.46613810674639 2 15.62357354300934 14.645871884592406 3 6.162806109479934 8.665719319644309 4 2.8912972116287383 5.420727649179978 5 1.7556938100096817 4.114562289348829 6 1.0236609113860258 2.8788048752392665 7 0.7039274307353073 2.309568184497971 8 0.4714012449129613 1.767606423774101 9 0.34717085192012853 1.464504207299576 >10 1.5837517880214733 13.074187238092849 >50 0.10847810633641128 3.5046853990966444 >100 0.055811785481084605 4.620060790864657 >500 0.003494666518049011 1.1072795881874367 >1k 0.001690967670023715 1.331102662035594 >5k 0.0 0.0 >10k+ 3.38193534004743E-4 2.6291813814000125 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20105 1.0605295737678628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17466 0.9213235282481719 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12188 0.6429114372087896 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3528 0.18610038976637755 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3150 0.16616106229140853 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2629 0.13867855008384541 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2108 0.11119603787628227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.2749543584574135E-5 0.0 0.0 0.0 0.0 6 5.2749543584574135E-5 0.0 0.0 0.0 0.0 7 5.2749543584574135E-5 0.0 0.0 0.0 0.0 8 5.2749543584574135E-5 0.0 0.0 0.0 0.0 9 5.2749543584574135E-5 0.0 0.0 0.0 0.0 10-11 5.2749543584574135E-5 0.0 0.0 0.0 0.0 12-13 5.2749543584574135E-5 0.0 0.0 0.0 0.0 14-15 5.2749543584574135E-5 0.0 0.0 0.0 0.0 16-17 5.2749543584574135E-5 0.0 0.0 5.2749543584574135E-5 0.0 18-19 5.2749543584574135E-5 0.0 0.0 1.0549908716914827E-4 0.0 20-21 5.2749543584574135E-5 0.0 0.0 1.0549908716914827E-4 0.0 22-23 5.2749543584574135E-5 0.0 0.0 2.901224897151577E-4 0.0 24-25 5.2749543584574135E-5 0.0 0.0 4.219963486765931E-4 0.0 26-27 5.2749543584574135E-5 0.0 0.0 6.593692948071766E-4 0.0 28-29 1.0549908716914827E-4 0.0 0.0 0.001239614274237492 0.0 30-31 1.0549908716914827E-4 0.0 0.0 0.0028484753535670034 0.0 32-33 1.0549908716914827E-4 0.0 0.0 0.005011206640534543 0.0 34-35 1.3187385896143533E-4 0.0 0.0 0.007411310873632665 0.0 36-37 1.582486307537224E-4 0.0 0.0 0.010497159173330252 0.0 38-39 1.582486307537224E-4 0.0 0.0 0.0159039873907491 0.0 40-41 1.582486307537224E-4 0.0 0.0 0.026295647476910204 0.0 42-43 2.1099817433829654E-4 0.0 0.0 0.03560594191958754 0.0 44-45 3.692468050920189E-4 0.0 0.0 0.046788845159517256 0.0 46-47 4.219963486765931E-4 0.0 0.0 0.06651717446014799 0.0 48-49 4.219963486765931E-4 0.0 0.0 0.08162991869712848 0.0 50-51 4.219963486765931E-4 0.0 0.0 0.10030325712606772 0.0 52-53 4.219963486765931E-4 0.0 0.0 0.12272181314951172 0.0 54-55 4.219963486765931E-4 0.0 0.0 0.1543715393002562 0.0 56-57 5.802449794303154E-4 0.0 0.0 0.22389543774472492 0.0 58-59 5.802449794303154E-4 0.0 0.0 0.2805220727827652 0.0 60-61 5.802449794303154E-4 0.0 0.0 0.32857690698831227 0.0 62-63 5.802449794303154E-4 0.0 0.0 0.3856519131468215 0.0 64-65 5.802449794303154E-4 0.0 0.0 0.44148730503109324 0.0 66-67 5.802449794303154E-4 0.0 0.0 0.5329550136067448 0.0 68-69 6.329945230148896E-4 0.0 0.0 0.6462874079982024 0.0 70-71 7.121188383917509E-4 0.0 0.0 0.7169717964015316 0.0 72-73 7.64868381976325E-4 0.0 0.0 0.7961752360937697 0.0 74-75 7.912431537686121E-4 0.0 0.0 0.9288403382089736 0.0 76-77 7.912431537686121E-4 0.0 0.0 1.082499758670838 0.0 78-79 7.912431537686121E-4 0.0 0.0 1.2409857623706912 0.0 80-81 8.967422409377603E-4 0.0 0.0 1.3954100512145318 0.0 82-83 9.494917845223345E-4 0.0 0.0 1.5978627994921273 0.0 84-85 0.0010549908716914827 0.0 0.0 1.882367462815528 0.0 86-87 0.0011604899588606309 0.0 0.0 2.1155995697747225 0.0 88-89 0.0011604899588606309 0.0 0.0 2.4337584419050815 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13675 0.0 33.987206 1 GTATCAA 20035 0.0 29.816032 1 GAGTACT 15280 0.0 24.184801 12-13 ATCAACG 24885 0.0 23.906984 3 TATAGCG 100 0.0016542039 23.749596 7 TATCAAC 25100 0.0 23.702202 2 TCAACGC 25215 0.0 23.5941 4 CAACGCA 25235 0.0 23.575405 5 AACGCAG 25710 0.0 23.259396 6 GTACATG 12650 0.0 23.179209 1 AGTACTT 15695 0.0 23.03083 12-13 TACATGG 12600 0.0 22.853893 2 GTACTTT 16330 0.0 22.425545 14-15 ACATGGG 12660 0.0 21.69463 3 ATACGTC 245 7.494236E-9 21.334608 5 ACGCAGA 28435 0.0 20.930716 7 CGCAGAG 28460 0.0 20.895641 8 GTATAGA 800 0.0 20.197279 1 GCAGAGT 29190 0.0 20.128984 9 TACTTTT 18060 0.0 19.856558 14-15 >>END_MODULE