FastQCFastQC Report
Thu 26 May 2016
SRR937586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890954
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150710.7970051095901857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122370.6471336690368988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82540.43649924852746286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.14267930367423004No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24930.13183821499624No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24380.12892963022897436No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22070.11671357420645874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.10666573591954114No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19350.10232930044834511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118000.031.1082421
GTATCAA172300.029.2247541
GTACATG132000.026.6561831
TACATGG132850.026.1538012
ATGGGCG2651.8189894E-1225.1081715
GAGTACT110550.024.25537512-13
ACATGGG135950.024.085823
ATCAACG209900.023.72853
TATCAAC211450.023.602642
TCAACGC211850.023.4895234
CAACGCA213250.023.3563615
AACGCAG217150.022.9909656
AGTACTT115900.022.3570312-13
GTACTTT119850.022.1948814-15
CATGGGA97250.021.2107544
CATGGGG35200.021.1988834
ACGCAGA238300.020.890637
CGCAGAG238100.020.8682778
GTGTAGC10500.020.830931
ATACCGT1404.8517674E-420.3581126
Produced by FastQC (version 0.11.5)