FastQCFastQC Report
Thu 26 May 2016
SRR937586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890954
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200941.0626382238806444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169390.8957912249584072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120530.6374031309064102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36550.1932886786246519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31670.1674815992350951No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26910.14230912015839625No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21340.1128530889699062No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19150.10127163326024853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133900.032.605661
GTATCAA191300.028.7326981
GTACATG120550.023.8421941
GAGTACT146550.023.32153112-13
ATCAACG237050.023.0073
TCAACGC238250.022.9309984
TACATGG123800.022.9092942
CAACGCA239300.022.829785
TATCAAC240900.022.7576312
AGTACTT150050.022.47679912-13
AACGCAG244550.022.3743786
GTACTTT155250.022.0428914-15
ACATGGG123050.021.8906943
GTGTAGC9800.020.8449291
CATGGGA90200.020.3300674
ACGCAGA271000.020.210277
CGCAGAG270700.020.1975738
TACTTTT171600.019.63818414-15
AACCGTC1707.954717E-519.5596547
AGAGTAC236750.019.48235110-11
Produced by FastQC (version 0.11.5)