##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937586_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1890954 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23631087800126 33.0 31.0 34.0 31.0 34.0 2 32.42477659424819 34.0 31.0 34.0 31.0 34.0 3 32.48878079530227 34.0 31.0 34.0 31.0 34.0 4 36.127806387675214 37.0 35.0 37.0 35.0 37.0 5 36.057887711705305 37.0 35.0 37.0 35.0 37.0 6 36.10590104254255 37.0 35.0 37.0 35.0 37.0 7 36.054711537139454 37.0 35.0 37.0 35.0 37.0 8 36.04534959602402 37.0 35.0 37.0 35.0 37.0 9 37.717027489827885 39.0 37.0 39.0 35.0 39.0 10-11 37.653703897609354 39.0 37.0 39.0 35.0 39.0 12-13 37.5560320346238 39.0 37.0 39.0 35.0 39.0 14-15 38.71576992354124 40.0 38.0 41.0 34.5 41.0 16-17 38.693122624876125 40.0 38.0 41.0 34.5 41.0 18-19 38.76451278032147 40.0 38.0 41.0 35.0 41.0 20-21 38.701262167138914 40.0 38.0 41.0 34.0 41.0 22-23 38.553749853248675 40.0 38.0 41.0 34.0 41.0 24-25 38.61096145120399 40.0 38.0 41.0 34.0 41.0 26-27 38.484379048882204 40.0 38.0 41.0 34.0 41.0 28-29 38.33433097790851 40.0 38.0 41.0 34.0 41.0 30-31 38.28422082187086 40.0 37.5 41.0 34.0 41.0 32-33 38.12460430026326 40.0 37.0 41.0 34.0 41.0 34-35 37.958573291576634 40.0 37.0 41.0 33.5 41.0 36-37 37.767157212708504 40.0 37.0 41.0 33.0 41.0 38-39 37.607868303512404 40.0 37.0 41.0 33.0 41.0 40-41 37.393371018015245 40.0 36.0 41.0 33.0 41.0 42-43 37.11755679937217 40.0 36.0 41.0 32.5 41.0 44-45 36.69214930664627 40.0 36.0 41.0 31.0 41.0 46-47 36.16544083039567 39.5 35.5 41.0 30.0 41.0 48-49 35.692778354206396 39.0 35.0 41.0 27.5 41.0 50-51 35.39476687428673 39.0 35.0 41.0 27.0 41.0 52-53 35.14249447633311 39.0 35.0 40.0 26.0 41.0 54-55 34.93553068979996 39.0 35.0 40.0 25.5 41.0 56-57 34.69564727645411 38.5 35.0 40.0 24.5 41.0 58-59 34.447559009896594 38.0 34.0 40.0 23.5 41.0 60-61 34.114198441633164 38.0 34.0 40.0 22.5 41.0 62-63 33.785295940567565 37.5 34.0 40.0 22.0 41.0 64-65 33.41864714847638 37.0 33.0 40.0 20.5 41.0 66-67 33.005831976875164 36.5 33.0 39.0 19.5 41.0 68-69 32.58361678813974 36.0 32.0 39.0 18.0 40.0 70-71 32.175120336084326 35.5 32.0 39.0 14.5 40.0 72-73 31.69094250838466 35.0 31.0 38.0 10.5 40.0 74-75 31.198557447722155 35.0 31.0 37.0 7.5 39.0 76-77 29.940907869784247 33.5 29.5 36.0 7.0 38.5 78-79 30.531471944849002 34.0 31.0 36.0 4.5 39.0 80-81 30.323874351253387 34.0 31.0 36.0 2.0 37.5 82-83 29.957479134870546 34.0 30.0 35.5 2.0 37.0 84-85 29.55566978361187 34.0 30.0 35.0 2.0 37.0 86-87 29.133280873040803 34.0 29.0 35.0 2.0 36.0 88-89 28.75221951459422 34.0 29.0 35.0 2.0 36.0 90-91 28.233185471460438 34.0 29.0 35.0 2.0 35.5 92-93 27.781562110976786 33.5 27.5 35.0 2.0 35.0 94-95 27.317065883146814 33.0 26.5 35.0 2.0 35.0 96-97 26.77946740111076 33.0 25.5 35.0 2.0 35.0 98-99 25.92669626019459 32.5 24.5 34.5 2.0 35.0 100-101 23.330058795718987 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3217147821696855 1101 2 0.2646592199494222 1101 3 0.25205632687171686 1101 4 0.11786562881701457 1101 5 0.06265197797958422 1101 6 0.17986331799284017 1101 7 0.17687895904690265 1101 8 0.17872338772296814 1101 9 0.29881878662293815 1101 10-11 0.3040707462926804 1101 12-13 0.2962550606850911 1101 14-15 0.3171616248684046 1101 16-17 0.34532979112956497 1101 18-19 0.3315235985941385 1101 20-21 0.38837536916305737 1101 22-23 0.3704429823979538 1101 24-25 0.3791479905636663 1101 26-27 0.27761225957323177 1101 28-29 0.33008776504676973 1101 30-31 0.3996471105032242 1101 32-33 0.4740238868020441 1101 34-35 0.4412746235565166 1101 36-37 0.4351453096279698 1101 38-39 0.4674055999251223 1101 40-41 0.424604171661521 1101 42-43 0.40921090177319286 1101 44-45 0.49365351433926463 1101 46-47 0.46768016653935973 1101 48-49 0.5805781197633451 1101 50-51 0.5035326658464214 1101 52-53 0.4535107224181516 1101 54-55 0.5300384156765787 1101 56-57 0.550606984515241 1101 58-59 0.6401825251418103 1101 60-61 0.7617161830966239 1101 62-63 0.6524018469877433 1101 64-65 0.6512822634973361 1101 66-67 0.5369224657245937 1101 68-69 0.6059560266723096 1101 70-71 0.6880558132486314 1101 72-73 0.6961491891617015 1101 74-75 0.645675225112992 1101 76-77 0.6146448724038649 1101 78-79 0.5974209162393578 1101 80-81 0.6079996699461532 1101 82-83 0.6126049309383852 1101 84-85 0.5752934716655353 1101 86-87 0.649649676202074 1101 88-89 0.8504946968982097 1101 90-91 0.7378068548355721 1101 92-93 0.7708959324665621 1101 94-95 0.6942772146568821 1101 96-97 0.919990203113553 1101 98-99 0.9361127235193436 1101 100-101 1.2134579838339157 1102 1 0.2503216964499799 1102 2 0.24714526307515428 1102 3 0.19712438020963674 1102 4 0.07265465145599848 1102 5 0.10269570219762159 1102 6 0.06818041371067807 1102 7 0.13170559965766415 1102 8 0.06747296876797293 1102 9 0.14821802761361624 1102 10-11 0.13807756266916726 1102 12-13 0.13802367812269978 1102 14-15 0.2562598274331762 1102 16-17 0.18596489413364026 1102 18-19 0.21490994899802018 1102 20-21 0.2845544996345595 1102 22-23 0.2687130653589307 1102 24-25 0.1138781884026443 1102 26-27 0.19174729741326502 1102 28-29 0.08813600876074901 1102 30-31 0.06274735074858029 1102 32-33 0.10968133124090684 1102 34-35 -9.82275154790102E-4 1102 36-37 0.16160246603452322 1102 38-39 0.18266764793001755 1102 40-41 0.3775014957131688 1102 42-43 0.3500104074487709 1102 44-45 0.2838289621326737 1102 46-47 0.3401160467064557 1102 48-49 0.32604036090619815 1102 50-51 0.2102686612215976 1102 52-53 0.23713105660650768 1102 54-55 0.3792902184875331 1102 56-57 0.1674622385688238 1102 58-59 0.2160580955698137 1102 60-61 0.12837673380916925 1102 62-63 0.22745285574399077 1102 64-65 0.4020203645081395 1102 66-67 0.2434956116864555 1102 68-69 0.3299818486189352 1102 70-71 0.19789910163213165 1102 72-73 0.26613863914084135 1102 74-75 0.2549822511065223 1102 76-77 0.26660582446376324 1102 78-79 0.13781218197732414 1102 80-81 0.1194963288635158 1102 82-83 0.27279972893108706 1102 84-85 0.24075781941479946 1102 86-87 0.2827493412565083 1102 88-89 0.29611719060083885 1102 90-91 0.18698242664694575 1102 92-93 0.21566207124387304 1102 94-95 0.21711265835843108 1102 96-97 0.31984448807963517 1102 98-99 0.26224003098752746 1102 100-101 0.6356950833572377 1103 1 0.2479092868716819 1103 2 0.25158380798308144 1103 3 0.18725564360349978 1103 4 0.15706733298626574 1103 5 0.1215183643652864 1103 6 0.16518810380463833 1103 7 0.2089059658614829 1103 8 0.1498685955567325 1103 9 0.2436855102603772 1103 10-11 0.2672727943824924 1103 12-13 0.14004748509639597 1103 14-15 0.255782165214292 1103 16-17 0.2524763603552458 1103 18-19 0.19661873407120822 1103 20-21 0.14304366206904717 1103 22-23 0.0904074518790523 1103 24-25 0.1734793270618269 1103 26-27 0.1845105343495561 1103 28-29 0.06840982807473495 1103 30-31 0.3551224973185825 1103 32-33 0.33948342564363543 1103 34-35 0.3818812813250716 1103 36-37 0.309733867267056 1103 38-39 0.2722605721494844 1103 40-41 0.24277117986702734 1103 42-43 0.32197607071821466 1103 44-45 0.4473546378448461 1103 46-47 0.5159065701115608 1103 48-49 0.551168862869936 1103 50-51 0.48793977442718983 1103 52-53 0.28857771071247384 1103 54-55 0.3575093801117575 1103 56-57 0.35311369858769126 1103 58-59 0.30698947519520914 1103 60-61 0.2683626146588125 1103 62-63 0.4963495596302039 1103 64-65 0.4478265763754976 1103 66-67 0.43836945670548744 1103 68-69 0.4221619255291884 1103 70-71 0.3791741838463736 1103 72-73 0.40907221859691845 1103 74-75 0.319485626756947 1103 76-77 0.32123359485806446 1103 78-79 0.45455240916898276 1103 80-81 0.6233347400306855 1103 82-83 0.6076252279676169 1103 84-85 0.5784613992154739 1103 86-87 0.6474004976577632 1103 88-89 0.6993740017404377 1103 90-91 0.5540777746849344 1103 92-93 0.6167727203056863 1103 94-95 0.8034342424972998 1103 96-97 0.9592762202181184 1103 98-99 0.8752425949730771 1103 100-101 1.2120757518561653 1104 1 0.1965172415519234 1104 2 0.24619283492727106 1104 3 0.10878086790119568 1104 4 0.14521976526994962 1104 5 0.1451169485973125 1104 6 0.14257472138123006 1104 7 0.15420200085713986 1104 8 0.19627461351952746 1104 9 0.2734927874216311 1104 10-11 0.3162158315464012 1104 12-13 0.27528416601247585 1104 14-15 0.434442333455344 1104 16-17 0.40730617403752234 1104 18-19 0.415305424239115 1104 20-21 0.3262742367740614 1104 22-23 0.3705528954689541 1104 24-25 0.25734638056589887 1104 26-27 0.30562772900341884 1104 28-29 0.24489178225206132 1104 30-31 0.3657357753623245 1104 32-33 0.4069728334787541 1104 34-35 0.43480545423474837 1104 36-37 0.4353970340515474 1104 38-39 0.4245041421241993 1104 40-41 0.5485267701103709 1104 42-43 0.4922909652235745 1104 44-45 0.3222633056280628 1104 46-47 0.3864080432883483 1104 48-49 0.36304086491956866 1104 50-51 0.426420053785165 1104 52-53 0.33590559099847184 1104 54-55 0.28091889479370735 1104 56-57 0.4440283966694736 1104 58-59 0.42605133913155413 1104 60-61 0.4764862001955805 1104 62-63 0.5932006145766167 1104 64-65 0.5553077524497709 1104 66-67 0.4269187689857574 1104 68-69 0.4467414414921578 1104 70-71 0.5390501618127743 1104 72-73 0.3780728780866909 1104 74-75 0.2513204791308752 1104 76-77 0.26736692091645153 1104 78-79 0.2967346086385412 1104 80-81 0.4656738648545442 1104 82-83 0.5913301371335749 1104 84-85 0.4934894631148161 1104 86-87 0.539454713410553 1104 88-89 0.6260681550552007 1104 90-91 0.7058968277714861 1104 92-93 0.7486048445744338 1104 94-95 0.8577473767817487 1104 96-97 0.9264008922524347 1104 98-99 1.0378746708476143 1104 100-101 1.2065297512583548 1105 1 0.19632851970093412 1105 2 0.2677343745493985 1105 3 0.1633426761996546 1105 4 0.06214985618695579 1105 5 0.024098557047409486 1105 6 0.07904105610264622 1105 7 0.1354302319303997 1105 8 0.13203879194241352 1105 9 0.2017976589936339 1105 10-11 0.19585430809683402 1105 12-13 0.2770795466162568 1105 14-15 0.23347929695103886 1105 16-17 0.23124130216275773 1105 18-19 0.2280147560048178 1105 20-21 0.21306723943206407 1105 22-23 0.15228976725289556 1105 24-25 0.21221927715617284 1105 26-27 0.0784481240830246 1105 28-29 0.1269100968498691 1105 30-31 0.22382438632660495 1105 32-33 0.2465400851915689 1105 34-35 0.08701782686957671 1105 36-37 0.2355844943928318 1105 38-39 0.24891662551957694 1105 40-41 0.2242888796573581 1105 42-43 0.3024332509025385 1105 44-45 0.48285349032570224 1105 46-47 0.4581817637706038 1105 48-49 0.2980096614771597 1105 50-51 0.2271775903594886 1105 52-53 0.3103341586425188 1105 54-55 0.3525494339251125 1105 56-57 0.4983849573398871 1105 58-59 0.4230354478938736 1105 60-61 0.5043151041581169 1105 62-63 0.48791400588052625 1105 64-65 0.6176997514506652 1105 66-67 0.5607085585785541 1105 68-69 0.48449539916345685 1105 70-71 0.5176094168156098 1105 72-73 0.47785678147523214 1105 74-75 0.44797520771075483 1105 76-77 0.41348847242200293 1105 78-79 0.598902639207445 1105 80-81 0.50094958672193 1105 82-83 0.5403342383016074 1105 84-85 0.6002936679789421 1105 86-87 0.642895354963926 1105 88-89 0.4682361351637212 1105 90-91 0.46784931679319897 1105 92-93 0.5604651803126259 1105 94-95 0.7608954742358982 1105 96-97 0.9613792762837399 1105 98-99 1.0442715342298996 1105 100-101 1.0918524643620309 1106 1 -0.029658920454508575 1106 2 0.01870436756004068 1106 3 0.04238954110718396 1106 4 0.05460408088938351 1106 5 0.055573757430387616 1106 6 0.05373764375870138 1106 7 0.03016915991587865 1106 8 0.05291024893844565 1106 9 0.04857563918774588 1106 10-11 -0.07028807372780221 1106 12-13 -0.14999270044213375 1106 14-15 0.02008857723105706 1106 16-17 0.16181347665047952 1106 18-19 0.10640572900038592 1106 20-21 0.027531585184476626 1106 22-23 0.09072542698593367 1106 24-25 0.22756804026580113 1106 26-27 0.2024585448643279 1106 28-29 0.19365858159257954 1106 30-31 0.23529462093328846 1106 32-33 0.244193677151209 1106 34-35 0.011258899036320713 1106 36-37 -0.08289197289301597 1106 38-39 0.03524572788202107 1106 40-41 0.004412992569449159 1106 42-43 -0.14354096642995984 1106 44-45 -0.1130457023157092 1106 46-47 -0.038164374983999494 1106 48-49 0.02047420880020212 1106 50-51 0.16162081319029653 1106 52-53 0.15931891722626546 1106 54-55 0.13711820533379182 1106 56-57 0.1008722023550277 1106 58-59 0.0571419847924588 1106 60-61 -0.010374092575077043 1106 62-63 0.010322202665946634 1106 64-65 0.1285154064541345 1106 66-67 0.17728265061181503 1106 68-69 0.23787505349609006 1106 70-71 0.2843546732787985 1106 72-73 0.214261197236695 1106 74-75 0.1673412628502966 1106 76-77 0.10913087248772158 1106 78-79 0.14322652330538688 1106 80-81 0.13084781192933548 1106 82-83 -0.011955622354822282 1106 84-85 0.05893153349301983 1106 86-87 0.33802083661404225 1106 88-89 -0.03809824792912764 1106 90-91 -0.031816963092335726 1106 92-93 -0.15752701879925368 1106 94-95 0.12480984451535804 1106 96-97 0.2188268252715666 1106 98-99 0.22099122954330142 1106 100-101 0.3118206067548819 1107 1 0.28307465620694927 1107 2 0.31467862778299605 1107 3 0.27855799044043295 1107 4 0.16735616616403348 1107 5 0.17835861691916932 1107 6 0.18111822682657674 1107 7 0.16579655243059932 1107 8 0.1796244055248053 1107 9 0.23282597341954414 1107 10-11 0.32619188487809225 1107 12-13 0.2521619233062822 1107 14-15 0.3567468645790228 1107 16-17 0.3658623521940214 1107 18-19 0.4046881820784378 1107 20-21 0.39117068701618507 1107 22-23 0.38988325598381124 1107 24-25 0.34136239909659594 1107 26-27 0.3278784080984849 1107 28-29 0.4397684261376824 1107 30-31 0.3654167195772118 1107 32-33 0.3509710598586935 1107 34-35 0.5009732319783353 1107 36-37 0.4173759072712784 1107 38-39 0.47727171676870483 1107 40-41 0.4439784417612671 1107 42-43 0.48712984275147164 1107 44-45 0.46228697254557716 1107 46-47 0.22964856463193684 1107 48-49 0.4314908496731462 1107 50-51 0.4877128941229927 1107 52-53 0.38970866322173947 1107 54-55 0.40982064911096217 1107 56-57 0.28884783151691096 1107 58-59 0.32187088308180734 1107 60-61 0.21253712603085262 1107 62-63 0.312399212465607 1107 64-65 0.43331108707928223 1107 66-67 0.41182985019462137 1107 68-69 0.31275927280801596 1107 70-71 0.47553526088652376 1107 72-73 0.6618508286883014 1107 74-75 0.4618802325025726 1107 76-77 0.2382188460215673 1107 78-79 0.5730211126207934 1107 80-81 0.6625200942334146 1107 82-83 0.6118939189167598 1107 84-85 0.7228817604563424 1107 86-87 0.8365143926482901 1107 88-89 0.8724534949943852 1107 90-91 1.0335437598843633 1107 92-93 1.1105981809104257 1107 94-95 1.2259201284945256 1107 96-97 1.3501419437237807 1107 98-99 1.505396442453467 1107 100-101 1.405345706005864 1108 1 0.17446781812493128 1108 2 0.17266574211421926 1108 3 0.15724201529472737 1108 4 0.0357136589322522 1108 5 0.015455954098754887 1108 6 0.0017660179735159431 1108 7 0.04900420244386794 1108 8 0.08272511629422041 1108 9 0.18908794877503254 1108 10-11 0.1681471398202703 1108 12-13 0.07652031936664372 1108 14-15 0.1714559110842444 1108 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-0.5227717543052179 2316 82-83 -0.6833140369915789 2316 84-85 -0.5674482764117137 2316 86-87 -0.5748172354320573 2316 88-89 -0.5147290566243399 2316 90-91 -0.505191429174463 2316 92-93 -0.29882288263780055 2316 94-95 -0.3117079472560569 2316 96-97 -0.15731509392725584 2316 98-99 0.04160551504229204 2316 100-101 0.7679256971428323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 7.0 5 28.0 6 86.0 7 196.0 8 459.0 9 1003.0 10 1763.0 11 3072.0 12 5126.0 13 7516.0 14 9709.0 15 14470.0 16 20700.0 17 31339.0 18 27619.0 19 13893.0 20 10301.0 21 9955.0 22 9971.0 23 10760.0 24 11881.0 25 14152.0 26 16953.0 27 20711.0 28 25946.0 29 32118.0 30 40562.0 31 51378.0 32 66235.0 33 87108.0 34 120357.0 35 175483.0 36 265149.0 37 407271.0 38 345102.0 39 32560.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40526728083543 20.294968149935176 15.924662153406077 20.375102415823317 2 15.105602780395506 21.595395763196777 40.961017560448326 22.337983895959393 3 18.885546660574505 26.56082591115384 31.74815463517357 22.805472793098087 4 11.884455900185568 18.062782232624993 38.69792363516943 31.354838232020004 5 12.091729192066921 39.37129905610227 34.29609666951414 14.240875082316663 6 29.098063728678753 38.431447830037115 17.604870345867745 14.865618095416389 7 26.266212715909536 35.78225594065218 20.64090400929901 17.310627334139276 8 26.276472087634072 35.09889717042297 19.66504737820169 18.959583363741263 9 26.742110067193597 16.55952498051248 21.092527898616254 35.60583705367767 10-11 24.194143325047392 28.173405639006987 26.969806502380944 20.66264453356468 12-13 25.871066296152623 25.972130003572396 28.219118387063425 19.937685313211553 14-15 22.61353264013826 26.41005545349067 28.315733751323407 22.660678155047663 16-17 19.994801565770505 30.049514689410746 28.807972060663563 21.147711684155194 18-19 19.820048504617247 27.364917390904274 33.25149633465436 19.563537769824123 20-21 21.13123851770059 26.03738641976484 33.53566506641621 19.29570999611836 22-23 20.625230706095508 26.295841102862394 33.312294347660995 19.7666338433811 24-25 20.161463472934827 26.35677546889031 33.84040542498654 19.641355633188326 26-27 20.233057766014127 26.569405020498984 33.6964669828221 19.501070230664787 28-29 19.834669223424477 26.72300206802291 33.89661683509732 19.545711873455296 30-31 20.028197407234654 26.641975426160553 33.62226156744162 19.707565599163175 32-33 19.87338137257702 26.65212903116628 33.459777445670284 20.014712150586423 34-35 20.27693428819527 26.557097634315802 33.43087668975554 19.735091387733387 36-37 20.342668303935476 26.29310390416689 33.49957217362242 19.864655618275222 38-39 20.210084655771272 26.73195829402333 33.41502058222525 19.64293646798015 40-41 20.21717781483168 26.872046110424503 33.300598274190754 19.610177800553057 42-43 20.19085444175314 26.873933405636297 32.84471972815777 20.090492424452787 44-45 20.357581411287637 27.10047415220042 32.38973555147296 20.15220888503898 46-47 20.495792071092158 27.387366376971624 31.675651549429546 20.44119000250667 48-49 20.68490296432383 27.69030870132219 31.17944698813403 20.44534134621995 50-51 20.75095428022046 28.005493523374973 30.842236246889136 20.401315949515432 52-53 20.915844594844717 28.243468640696705 30.3885234648754 20.452163299583173 54-55 20.598359346657823 28.139552839466216 30.489424914619818 20.77266289925614 56-57 20.85243744691833 28.432235792092246 30.09898707213396 20.61633968885547 58-59 20.994508591959402 28.062210926336657 30.318505897023407 20.624774584680537 60-61 20.883744395685987 27.99457310965788 30.715474834395764 20.406207660260375 62-63 20.907198165582027 27.879895544788504 30.79461478174509 20.41829150788438 64-65 20.80473665673517 27.95258372229044 30.69958338489461 20.543096236079776 66-67 20.73014467829466 28.237757237880984 30.34756530301636 20.68453278080799 68-69 20.917970991346692 28.159266782410775 30.26928749683361 20.653474729408927 70-71 20.921132930785202 28.178104808472337 30.223765358649658 20.676996902092807 72-73 20.91158748441263 28.1547039219357 30.11170552007082 20.82200307358085 74-75 20.77929976086145 28.491888221500894 29.81616686603693 20.912645151600724 76-77 20.77797767687633 28.241723489836346 30.12143605820131 20.858862775086013 78-79 20.86951877200609 28.28849882122992 29.892874178853635 20.949108227910358 80-81 20.712254614472172 28.584792958894273 29.80994239412614 20.893010032507416 82-83 20.78473794252137 28.510059830933386 29.67063767923565 21.034564547309593 84-85 20.69633947673267 28.32165178742592 29.687925960848688 21.294082774992724 86-87 20.691460500890027 28.288472379550218 29.819154775843305 21.200912343716453 88-89 20.756930099833205 28.13267800274359 29.683614725688727 21.42677717173448 90-91 20.501493336182318 28.1690487234316 29.944391936883736 21.385066003502345 92-93 20.503674166602764 28.17042633024927 30.06161071021548 21.264288792932483 94-95 20.39560454669971 28.08222727787138 29.93716399235518 21.58500418307373 96-97 20.37328776903087 27.99364765086829 29.91252034687253 21.720544233228306 98-99 20.277886337686954 28.059586962304532 29.698712317885978 21.963814382122536 100-101 20.181535730607134 27.793252060730183 30.23941707147814 21.785795137184543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1202.0 1 834.5 2 700.5 3 992.0 4 795.0 5 693.5 6 1497.0 7 3720.5 8 6076.0 9 6961.0 10 5932.0 11 4684.0 12 4751.0 13 5071.5 14 5281.5 15 5335.0 16 5547.5 17 5885.5 18 6152.5 19 6489.5 20 6962.5 21 7773.0 22 8792.5 23 10206.5 24 11755.5 25 13695.0 26 16823.5 27 20372.0 28 23156.5 29 25684.0 30 28773.5 31 32662.5 32 36968.0 33 41443.5 34 46654.0 35 50532.0 36 54765.0 37 59845.5 38 62173.5 39 62833.0 40 63857.5 41 64974.0 42 66458.5 43 68390.5 44 70015.0 45 70428.5 46 68919.5 47 69575.0 48 78851.5 49 76458.0 50 63352.5 51 57636.0 52 51955.5 53 47437.0 54 43773.5 55 38759.5 56 33822.0 57 29129.0 58 25791.5 59 23051.0 60 19644.5 61 15838.5 62 12043.0 63 8677.0 64 5928.0 65 4296.5 66 3059.0 67 2290.5 68 1671.0 69 1063.5 70 760.0 71 633.5 72 533.0 73 479.5 74 427.5 75 363.5 76 273.0 77 147.5 78 98.0 79 80.0 80 49.5 81 35.5 82 24.0 83 12.5 84 7.0 85 4.5 86 2.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01993702649562073 2 0.0 3 0.0 4 2.644167970241476E-4 5 0.020571626808478682 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.644167970241476E-5 12-13 0.0035696267598259927 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010576671880965904 24-25 0.0 26-27 3.437418361313919E-4 28-29 9.254587895845166E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.0576671880965904E-4 40-41 7.932503910724428E-4 42-43 0.015785682782341614 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.288335940482952E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.288335940482952E-5 82-83 0.0022475427747052543 84-85 4.495085549410509E-4 86-87 0.0 88-89 0.0 90-91 0.011687222428467323 92-93 0.0025912846108366466 94-95 0.0 96-97 0.0 98-99 0.0019566842979786923 100-101 0.019143776104548284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1890954.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.908577003542966 #Duplication Level Percentage of deduplicated Percentage of total 1 69.50094373931597 32.60190371214611 2 15.37425710602853 14.423690466608138 3 6.132080655803591 8.62941532904097 4 3.020837373511838 5.668127302022423 5 1.644696102003378 3.857517687412621 6 1.053718258608264 2.965705444437898 7 0.6896403498530377 2.2645033219082076 8 0.4866405720852306 1.8262093398966592 9 0.33777834010195096 1.4260231149121165 >10 1.5874709364269373 13.09015379140945 >50 0.11045648582008968 3.549520547951342 >100 0.05605623251136966 4.6435545970788885 >500 0.00327690812427625 1.0377359209154196 >1k 0.0018079493099455173 1.414125887940672 >5k 0.0 0.0 >10k+ 3.389904956147845E-4 2.601813536319046 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20094 1.0626382238806444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16939 0.8957912249584072 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12053 0.6374031309064102 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3655 0.1932886786246519 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3167 0.1674815992350951 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2691 0.14230912015839625 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2134 0.1128530889699062 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1915 0.10127163326024853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.288335940482952E-5 0.0 0.0 0.0 0.0 7 5.288335940482952E-5 0.0 0.0 0.0 0.0 8 5.288335940482952E-5 0.0 0.0 0.0 0.0 9 5.288335940482952E-5 0.0 0.0 0.0 0.0 10-11 5.288335940482952E-5 0.0 0.0 0.0 0.0 12-13 5.288335940482952E-5 0.0 0.0 0.0 0.0 14-15 1.0576671880965904E-4 0.0 0.0 0.0 0.0 16-17 1.0576671880965904E-4 0.0 0.0 2.644167970241476E-5 0.0 18-19 1.5865007821448855E-4 0.0 0.0 5.288335940482952E-5 0.0 20-21 1.5865007821448855E-4 0.0 0.0 1.322083985120738E-4 0.0 22-23 2.1153343761931808E-4 0.0 0.0 2.3797511732173284E-4 0.0 24-25 2.1153343761931808E-4 0.0 0.0 3.7018351583380666E-4 0.0 26-27 2.1153343761931808E-4 0.0 0.0 7.139253519651985E-4 0.0 28-29 2.908584767265624E-4 0.0 0.0 0.0015600591024424707 0.0 30-31 3.173001564289771E-4 0.0 0.0 0.0035696267598259923 0.0 32-33 3.173001564289771E-4 0.0 0.0 0.006213794730067468 0.0 34-35 3.173001564289771E-4 0.0 0.0 0.008408454145367894 0.0 36-37 3.173001564289771E-4 0.0 0.0 0.012189614342813205 0.0 38-39 3.7018351583380666E-4 0.0 0.0 0.017451508603593742 0.0 40-41 3.7018351583380666E-4 0.0 0.0 0.028953639274144162 0.0 42-43 3.7018351583380666E-4 0.0 0.0 0.04019135314767043 0.0 44-45 3.7018351583380666E-4 0.0 0.0 0.051746367177625686 0.0 46-47 5.023919143458805E-4 2.644167970241476E-5 0.0 0.07482995355783377 0.0 48-49 6.346003128579542E-4 5.288335940482952E-5 0.0 0.09072140305898505 0.0 50-51 6.346003128579542E-4 5.288335940482952E-5 0.0 0.10960076236650917 0.0 52-53 6.346003128579542E-4 5.288335940482952E-5 0.0 0.13633330054565052 0.0 54-55 6.346003128579542E-4 7.932503910724429E-5 0.0 0.17229398494093456 0.0 56-57 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.24762633041311422 0.0 58-59 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.30841575204896576 0.0 60-61 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.3589722436399828 0.0 62-63 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.4187304397674402 0.0 64-65 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.4783035441369806 0.0 66-67 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.5739166579409123 0.0 68-69 6.346003128579542E-4 1.0576671880965904E-4 0.0 0.696553168400712 0.0 70-71 7.139253519651985E-4 1.0576671880965904E-4 0.0 0.7696379710981864 0.0 72-73 7.668087113700281E-4 1.0576671880965904E-4 0.0 0.8513163196989456 0.0 74-75 7.932503910724428E-4 1.0576671880965904E-4 0.0 0.9934139064197225 0.0 76-77 7.932503910724428E-4 1.0576671880965904E-4 0.0 1.1537033687757607 0.0 78-79 7.932503910724428E-4 1.0576671880965904E-4 0.0 1.3209998762529391 0.0 80-81 8.990171098821018E-4 1.0576671880965904E-4 0.0 1.4739649933314083 0.0 82-83 8.990171098821018E-4 1.0576671880965904E-4 0.0 1.6788351276657179 0.0 84-85 8.990171098821018E-4 1.0576671880965904E-4 0.0 1.9681335452898379 0.0 86-87 8.990171098821018E-4 1.0576671880965904E-4 0.0 2.2112119067941367 0.0 88-89 8.990171098821018E-4 1.0576671880965904E-4 0.0 2.5345936495546693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13390 0.0 32.60566 1 GTATCAA 19130 0.0 28.732698 1 GTACATG 12055 0.0 23.842194 1 GAGTACT 14655 0.0 23.321531 12-13 ATCAACG 23705 0.0 23.007 3 TCAACGC 23825 0.0 22.930998 4 TACATGG 12380 0.0 22.909294 2 CAACGCA 23930 0.0 22.82978 5 TATCAAC 24090 0.0 22.757631 2 AGTACTT 15005 0.0 22.476799 12-13 AACGCAG 24455 0.0 22.374378 6 GTACTTT 15525 0.0 22.04289 14-15 ACATGGG 12305 0.0 21.890694 3 GTGTAGC 980 0.0 20.844929 1 CATGGGA 9020 0.0 20.330067 4 ACGCAGA 27100 0.0 20.21027 7 CGCAGAG 27070 0.0 20.197573 8 TACTTTT 17160 0.0 19.638184 14-15 AACCGTC 170 7.954717E-5 19.559654 7 AGAGTAC 23675 0.0 19.482351 10-11 >>END_MODULE