FastQCFastQC Report
Thu 26 May 2016
SRR937585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792872
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150160.8375388761718628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118270.6596678402027585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81820.45636275205368815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.13626181902556345No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23800.13274790392175237No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23350.1302379645618873No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22170.1236563457960189No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19250.10736962817200557No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19150.10681186386981335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA165800.030.2219621
GGTATCA112300.030.0146371
GTCGGGA3450.026.1760392
GTACATG125600.025.2087441
ATCAACG200000.024.8534163
TATCAAC200650.024.825132
TCAACGC202850.024.553834
CAACGCA203700.024.452745
TACATGG127750.024.369682
GAGTACT105850.024.00683412-13
AACGCAG208200.023.9578196
ACATGGG127500.023.443633
GTGTAGC9750.023.4047071
AGTACTT110450.022.5339612-13
GTACTTT115550.021.99093214-15
CATGGGA94200.021.8963534
ACGCAGA230050.021.620377
CGCAGAG229400.021.6195078
CGGGAGT4300.020.9999434
ATGGGAT36600.020.777645
Produced by FastQC (version 0.11.5)