##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937585_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792872 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82476272706585 33.0 31.0 34.0 30.0 34.0 2 31.933865886689066 33.0 31.0 34.0 30.0 34.0 3 31.935284281309542 33.0 31.0 34.0 30.0 34.0 4 35.55384433467643 37.0 35.0 37.0 33.0 37.0 5 35.565954513205625 37.0 35.0 37.0 33.0 37.0 6 35.6348975275424 37.0 35.0 37.0 33.0 37.0 7 35.57944013850403 37.0 35.0 37.0 33.0 37.0 8 35.57325732121423 37.0 35.0 37.0 33.0 37.0 9 37.197837882458984 39.0 37.0 39.0 34.0 39.0 10-11 37.09023482992651 39.0 37.0 39.0 33.0 39.0 12-13 36.9754870955651 39.0 37.0 39.0 33.0 39.0 14-15 38.085964307546774 40.0 38.0 41.0 33.0 41.0 16-17 38.04502106118005 40.0 38.0 41.0 33.0 41.0 18-19 38.058228083209514 40.0 38.0 41.0 33.0 41.0 20-21 38.017948297480245 40.0 38.0 41.0 33.0 41.0 22-23 37.830149056932115 40.0 37.5 41.0 33.0 41.0 24-25 37.66927254148651 40.0 37.0 41.0 32.0 41.0 26-27 37.61054163375857 40.0 37.0 41.0 32.5 41.0 28-29 37.64313152305351 40.0 37.0 41.0 33.0 41.0 30-31 37.43212621983053 39.5 37.0 41.0 32.0 41.0 32-33 37.16643603112771 39.0 36.0 41.0 32.0 41.0 34-35 37.14737527274674 39.5 36.0 41.0 32.0 41.0 36-37 37.03981014818682 40.0 36.0 41.0 31.0 41.0 38-39 36.899813539393776 39.5 36.0 41.0 31.0 41.0 40-41 36.65082309278074 39.0 35.5 41.0 30.5 41.0 42-43 36.36562565537305 39.0 35.0 41.0 30.0 41.0 44-45 36.04934289787559 39.0 35.0 41.0 29.5 41.0 46-47 35.58300006916277 39.0 35.0 41.0 27.5 41.0 48-49 35.14473760536168 39.0 35.0 40.0 25.0 41.0 50-51 34.18019496093419 38.0 34.0 39.5 23.5 40.5 52-53 34.27074687986649 38.0 34.0 39.5 23.5 40.5 54-55 34.63275543373983 39.0 35.0 40.0 23.5 41.0 56-57 34.458310186114794 38.0 34.0 40.0 23.0 41.0 58-59 34.243604116746766 38.0 34.0 40.0 22.0 41.0 60-61 33.943615048927086 38.0 34.0 40.0 21.0 41.0 62-63 33.61117748506307 38.0 33.5 40.0 19.5 41.0 64-65 33.26630428719953 37.0 33.0 40.0 18.0 41.0 66-67 32.877002373844874 36.5 32.5 39.5 15.0 41.0 68-69 32.41842697080439 36.0 32.0 39.0 10.5 40.5 70-71 31.987692372907823 35.5 31.5 39.0 8.0 40.0 72-73 31.540521855436417 35.0 31.0 38.0 7.0 40.0 74-75 31.099006510224935 35.0 31.0 37.5 2.0 39.5 76-77 30.60411144800075 35.0 30.5 37.0 2.0 39.0 78-79 30.081672032359254 34.5 30.0 36.0 2.0 39.0 80-81 29.556316903828048 34.0 29.0 36.0 2.0 38.0 82-83 29.032715665145084 34.0 29.0 35.0 2.0 37.0 84-85 28.563573696281722 34.0 28.5 35.0 2.0 37.0 86-87 28.097655605085027 34.0 27.5 35.0 2.0 36.0 88-89 27.617648387614956 33.5 26.5 35.0 2.0 36.0 90-91 27.117620499399848 33.0 26.0 35.0 2.0 35.5 92-93 26.57848496713653 33.0 25.0 35.0 2.0 35.0 94-95 25.965240965333834 32.5 23.5 35.0 2.0 35.0 96-97 25.258450408060362 32.0 19.5 34.0 2.0 35.0 98-99 24.3517713478709 32.0 8.5 34.0 2.0 35.0 100-101 22.048820272724434 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15523664797007442 1101 2 0.2675903318361499 1101 3 0.1667287854492514 1101 4 0.17013622209856294 1101 5 -0.004271115236569756 1101 6 0.1612097080798236 1101 7 0.05742771194996976 1101 8 0.039246996499961995 1101 9 0.17070501848699848 1101 10-11 0.203395922686326 1101 12-13 0.3852624904853883 1101 14-15 0.3587141694818712 1101 16-17 0.4002909317657881 1101 18-19 0.31740644861324085 1101 20-21 0.37161656177446645 1101 22-23 0.30730873395436475 1101 24-25 0.26825457238904704 1101 26-27 0.3013132730054764 1101 28-29 0.20460792713118536 1101 30-31 0.28803523272173237 1101 32-33 0.2285404777276696 1101 34-35 0.30914515522973574 1101 36-37 0.3928943174264674 1101 38-39 0.512981248605989 1101 40-41 0.5892651817271641 1101 42-43 0.4543416849815216 1101 44-45 0.5659287619196576 1101 46-47 0.5858522458043467 1101 48-49 0.8117550037625136 1101 50-51 0.6524180591397055 1101 52-53 0.8038297359017506 1101 54-55 0.8697720963122535 1101 56-57 0.9221336207278341 1101 58-59 0.8855919593272716 1101 60-61 0.9082537516330262 1101 62-63 1.0021177605634222 1101 64-65 0.782583743963329 1101 66-67 0.9658570580856392 1101 68-69 0.9461629235300748 1101 70-71 1.0655649781934322 1101 72-73 0.9491462982425425 1101 74-75 0.8308814903725974 1101 76-77 0.9314772844128179 1101 78-79 0.9973514084644393 1101 80-81 0.8840962191058885 1101 82-83 0.9897249668097139 1101 84-85 1.2178336464828625 1101 86-87 1.116327836568768 1101 88-89 1.1042026576352342 1101 90-91 1.1904961888849463 1101 92-93 1.378325224857786 1101 94-95 1.5352736266153961 1101 96-97 1.5740393747480397 1101 98-99 1.674297382316972 1101 100-101 1.9471131468936136 1102 1 0.22340820117511484 1102 2 0.2009387848076436 1102 3 0.2846291502507299 1102 4 0.21441713854165556 1102 5 0.25941183560208714 1102 6 0.26103502800493317 1102 7 0.2344683805596759 1102 8 0.27737261174055305 1102 9 0.3726303596439422 1102 10-11 0.34521153387357373 1102 12-13 0.30004129339608454 1102 14-15 0.4022514892525635 1102 16-17 0.48077887787554374 1102 18-19 0.5499606380397921 1102 20-21 0.5785926663122964 1102 22-23 0.7394638614418696 1102 24-25 0.6262223000039384 1102 26-27 0.5508099820714705 1102 28-29 0.5038537145426432 1102 30-31 0.7053406236769746 1102 32-33 0.6643011563742363 1102 34-35 0.7438288009283625 1102 36-37 0.7327860350816948 1102 38-39 0.8309771566528568 1102 40-41 0.8667415854408134 1102 42-43 0.7353276140671952 1102 44-45 0.9430582181917373 1102 46-47 0.9591368102622582 1102 48-49 0.9378523034594792 1102 50-51 0.8186623410212306 1102 52-53 0.8051991890855632 1102 54-55 0.8686197868679457 1102 56-57 0.7917450781966124 1102 58-59 0.8157966534923773 1102 60-61 0.8719656549702819 1102 62-63 1.0146329606251925 1102 64-65 0.971928259395014 1102 66-67 1.0476864699638853 1102 68-69 0.9805449807016089 1102 70-71 1.052545522114368 1102 72-73 1.1459948187698679 1102 74-75 1.0329269843987277 1102 76-77 0.9479425703669939 1102 78-79 1.0964312015505833 1102 80-81 1.1756642864301021 1102 82-83 1.099083379440728 1102 84-85 1.2271978496135922 1102 86-87 1.2678574935781697 1102 88-89 1.2548772508526298 1102 90-91 1.3783843936370168 1102 92-93 1.6824282764511374 1102 94-95 1.6187494705807275 1102 96-97 1.9116033115157869 1102 98-99 2.0617426138404547 1102 100-101 2.3962797974755574 1103 1 0.31378057428559814 1103 2 0.19943823177827014 1103 3 0.29048020100653815 1103 4 0.23091584813978727 1103 5 0.1931453983401994 1103 6 0.20009487807252668 1103 7 0.2177226511753787 1103 8 0.3009881948793023 1103 9 0.23760639503782244 1103 10-11 0.1984836243246022 1103 12-13 0.22570615759664747 1103 14-15 0.3380927083750933 1103 16-17 0.33500832361720967 1103 18-19 0.21237184610173188 1103 20-21 0.2390615123243336 1103 22-23 0.38151375698075896 1103 24-25 0.49438009771808566 1103 26-27 0.3619658134589585 1103 28-29 0.12921748497400642 1103 30-31 0.17604665784190132 1103 32-33 0.16967783082428411 1103 34-35 0.3344395911458875 1103 36-37 0.39315226342137777 1103 38-39 0.4359279984864557 1103 40-41 0.5879281406823011 1103 42-43 0.4336586943179057 1103 44-45 0.316510964904289 1103 46-47 0.33718400210199917 1103 48-49 0.5643854237740911 1103 50-51 0.4003490806058494 1103 52-53 0.3854185574031206 1103 54-55 0.6344327400447938 1103 56-57 0.46200930334101287 1103 58-59 0.5411647252632932 1103 60-61 0.45969762006798476 1103 62-63 0.5338697800918197 1103 64-65 0.6776170882016999 1103 66-67 0.6447701617421799 1103 68-69 0.7029709362134682 1103 70-71 0.67542619066543 1103 72-73 0.6777085953459938 1103 74-75 0.6238413262937783 1103 76-77 0.6647896884694902 1103 78-79 0.6870100389599472 1103 80-81 0.6768354797445895 1103 82-83 0.7959243527724205 1103 84-85 0.8413701489868295 1103 86-87 1.0479988233065711 1103 88-89 1.0413501524134041 1103 90-91 1.143377634389136 1103 92-93 1.030685619452246 1103 94-95 1.3241077107183692 1103 96-97 1.6446498888784475 1103 98-99 1.7464906782376524 1103 100-101 2.2037272596852198 1104 1 0.21713980873041905 1104 2 0.1853056395821362 1104 3 0.2850122076870889 1104 4 0.1148560842805395 1104 5 0.19713025747469004 1104 6 0.21782619864230668 1104 7 0.32329962261537304 1104 8 0.1589893169025558 1104 9 0.2325433618349635 1104 10-11 0.2304658009207401 1104 12-13 0.22550200095189155 1104 14-15 0.42989394260067115 1104 16-17 0.435754791500905 1104 18-19 0.4854101432057476 1104 20-21 0.42637259004701633 1104 22-23 0.5240025851113046 1104 24-25 0.4218432289367655 1104 26-27 0.479317838581963 1104 28-29 0.36832025327620244 1104 30-31 0.5180583036694557 1104 32-33 0.4156034032818354 1104 34-35 0.5271632058663087 1104 36-37 0.6253014538235675 1104 38-39 0.5875110185345633 1104 40-41 0.5982228974805324 1104 42-43 0.7841650879159161 1104 44-45 0.6905737853096312 1104 46-47 0.6664119340325954 1104 48-49 0.7578222325851982 1104 50-51 0.7906238473346292 1104 52-53 0.7878759172953025 1104 54-55 0.8230569718780103 1104 56-57 0.7952383443980366 1104 58-59 0.8534094810942676 1104 60-61 0.9164249232786474 1104 62-63 0.8993489936437982 1104 64-65 0.7924810590033076 1104 66-67 0.8765606916079847 1104 68-69 0.9963356518063691 1104 70-71 1.109531312637099 1104 72-73 1.0728898278949863 1104 74-75 1.1605642445859843 1104 76-77 1.1408824681853709 1104 78-79 1.136131185229221 1104 80-81 1.1223807250098439 1104 82-83 1.4200225749459214 1104 84-85 1.4080588262245861 1104 86-87 1.662182357937752 1104 88-89 1.73144101050325 1104 90-91 1.6207959491617956 1104 92-93 1.526528845720481 1104 94-95 1.5293934719853546 1104 96-97 1.8722361847185773 1104 98-99 2.1293648633199 1104 100-101 2.4642384895903646 1105 1 0.09040318768930788 1105 2 0.012414241681749871 1105 3 0.1467564646187256 1105 4 0.0816194986883545 1105 5 0.09363526699662827 1105 6 0.06638318094494622 1105 7 0.11232348508119827 1105 8 -0.03999303883878724 1105 9 0.08504434523473492 1105 10-11 0.18809653149986616 1105 12-13 0.22997417737640546 1105 14-15 0.2855431739611731 1105 16-17 0.3573191813975001 1105 18-19 0.2587204132937231 1105 20-21 0.30949172882696274 1105 22-23 0.4156098942147253 1105 24-25 0.37471121138366925 1105 26-27 0.34715616300561436 1105 28-29 0.2542352037722537 1105 30-31 0.37473240594248836 1105 32-33 0.43923346649042116 1105 34-35 0.46636131554884486 1105 36-37 0.44486326676108234 1105 38-39 0.42265338772185856 1105 40-41 0.415161763547367 1105 42-43 0.5042758254370128 1105 44-45 0.4258395550865046 1105 46-47 0.453845522041604 1105 48-49 0.4517074705843669 1105 50-51 0.3635618626272148 1105 52-53 0.4030037674780047 1105 54-55 0.47138007338266874 1105 56-57 0.41940950331517257 1105 58-59 0.49744969523316485 1105 60-61 0.5129104701077765 1105 62-63 0.6552502819317283 1105 64-65 0.640024804211194 1105 66-67 0.8029086336403708 1105 68-69 0.7840090333301717 1105 70-71 0.8510894722820943 1105 72-73 0.9103991999617271 1105 74-75 0.958514803871509 1105 76-77 0.9519558370625312 1105 78-79 0.9913817188108496 1105 80-81 1.0395439732046192 1105 82-83 1.0318167204255815 1105 84-85 1.1947520928172146 1105 86-87 1.3520566661704159 1105 88-89 1.3857399251638611 1105 90-91 1.378001730594388 1105 92-93 1.504211898368574 1105 94-95 1.533684023984005 1105 96-97 1.5488105755924124 1105 98-99 1.6579787171986027 1105 100-101 1.9712681897690842 1106 1 0.10620644562251513 1106 2 0.1888746805384578 1106 3 0.1838112223347821 1106 4 0.186906831319952 1106 5 0.16370894958001259 1106 6 0.04260571559637327 1106 7 0.10352809594736101 1106 8 -0.0030104179176433377 1106 9 0.1758342431390858 1106 10-11 -0.0368519054135632 1106 12-13 0.013235202160551296 1106 14-15 0.027858617860090362 1106 16-17 -0.09331142668623471 1106 18-19 0.16424977223316972 1106 20-21 0.30242894773269313 1106 22-23 0.16757930232495966 1106 24-25 0.08650425239122939 1106 26-27 0.13129475398262969 1106 28-29 0.2641403218042413 1106 30-31 0.28528543016719965 1106 32-33 0.43921138379809577 1106 34-35 0.29496326029795483 1106 36-37 0.17910336451380005 1106 38-39 0.31919832968118556 1106 40-41 0.391822713250356 1106 42-43 0.38820698632414263 1106 44-45 0.37668436528576166 1106 46-47 0.5628992786756086 1106 48-49 0.64216936689877 1106 50-51 0.641920088062065 1106 52-53 0.7987322386932263 1106 54-55 0.6053188159941598 1106 56-57 0.7360408622104444 1106 58-59 0.7011404492098876 1106 60-61 0.8989832104962829 1106 62-63 1.0575389321639648 1106 64-65 0.9762333531574825 1106 66-67 1.0734332448009596 1106 68-69 0.9738951602467623 1106 70-71 1.1597299881337868 1106 72-73 0.9959329754396329 1106 74-75 1.1477254948389515 1106 76-77 1.098557678759068 1106 78-79 1.2222484644844833 1106 80-81 1.1005705239616859 1106 82-83 0.8744504495298351 1106 84-85 0.9640405148616331 1106 86-87 1.0870555252313139 1106 88-89 1.1829142238543575 1106 90-91 1.2837348391749863 1106 92-93 1.3009613260588004 1106 94-95 1.70572380227377 1106 96-97 1.8265122089755543 1106 98-99 1.9177374269805014 1106 100-101 2.585943316560545 1107 1 0.1235294958799571 1107 2 0.0983495299700472 1107 3 0.16275713422950844 1107 4 0.06550827473754595 1107 5 0.03318146856947379 1107 6 0.028281070442595535 1107 7 0.13395842665165958 1107 8 0.21132325769937665 1107 9 0.1645849076792274 1107 10-11 0.3041195976487785 1107 12-13 0.25597944341956946 1107 14-15 0.361145488003487 1107 16-17 0.3450623616659172 1107 18-19 0.28616117173648803 1107 20-21 0.34729360002344123 1107 22-23 0.3051059749633893 1107 24-25 0.37163297290567243 1107 26-27 0.33113249694942226 1107 28-29 0.3628131350120256 1107 30-31 0.38141937232853707 1107 32-33 0.40433486081775527 1107 34-35 0.4674250082752067 1107 36-37 0.3811299752498556 1107 38-39 0.43160414704735217 1107 40-41 0.37596682196548414 1107 42-43 0.6175210352488634 1107 44-45 0.5717749976366733 1107 46-47 0.5349421296802532 1107 48-49 0.4540428238911858 1107 50-51 0.43695551806504795 1107 52-53 0.5162105546574836 1107 54-55 0.5452656527831081 1107 56-57 0.7506008820192065 1107 58-59 0.6222841136844792 1107 60-61 0.6911009269543058 1107 62-63 0.7033698440222693 1107 64-65 0.825186183552276 1107 66-67 0.7123517740006733 1107 68-69 0.8315481137180285 1107 70-71 1.0241744325782314 1107 72-73 1.0547080367958053 1107 74-75 1.0392314702382208 1107 76-77 0.9992568113976397 1107 78-79 1.0886905347910094 1107 80-81 0.968962883024556 1107 82-83 1.0478228177688216 1107 84-85 1.1574931377014188 1107 86-87 1.2643453666884241 1107 88-89 1.3592637319125913 1107 90-91 1.4062777884951494 1107 92-93 1.2101993443090713 1107 94-95 1.2477626478310029 1107 96-97 1.6110985190845746 1107 98-99 1.8701709183469397 1107 100-101 2.3099231291056057 1108 1 0.06269356334580323 1108 2 0.06095627195173492 1108 3 0.09253339127235449 1108 4 0.10131345586327001 1108 5 0.10146167136822726 1108 6 0.05150609119215943 1108 7 -0.0034714574377616714 1108 8 -0.019221805864901853 1108 9 0.08520890909311873 1108 10-11 -0.0011767481831626014 1108 12-13 0.14804363366373252 1108 14-15 0.1356789176045723 1108 16-17 0.22435416859281787 1108 18-19 0.3060523391704848 1108 20-21 0.3407865764273339 1108 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-0.4287202880678649 2316 64-65 -0.45660941566298163 2316 66-67 -0.5134194273178068 2316 68-69 -0.47171036769601926 2316 70-71 -0.4209507470602212 2316 72-73 -0.36898846025886556 2316 74-75 -0.4033842900300826 2316 76-77 -0.503437901124741 2316 78-79 -0.5851618509849885 2316 80-81 -0.42917256844112117 2316 82-83 -0.44068593063746064 2316 84-85 -0.49057925256938617 2316 86-87 -0.45120738775580804 2316 88-89 -0.6292269214592956 2316 90-91 -0.4811340027359243 2316 92-93 -0.33701530514125366 2316 94-95 -0.3168708245807643 2316 96-97 -0.21497906735631034 2316 98-99 -0.25319704526322084 2316 100-101 -0.015522035883108742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6428.0 3 1060.0 4 964.0 5 1113.0 6 1283.0 7 1626.0 8 1964.0 9 2456.0 10 3298.0 11 4477.0 12 6430.0 13 9259.0 14 10367.0 15 14048.0 16 19252.0 17 26620.0 18 22322.0 19 13004.0 20 11805.0 21 12075.0 22 12867.0 23 13886.0 24 15861.0 25 18211.0 26 20870.0 27 23974.0 28 29074.0 29 34960.0 30 42991.0 31 53144.0 32 67566.0 33 88778.0 34 119332.0 35 170752.0 36 244813.0 37 343456.0 38 285178.0 39 37304.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96326567829107 19.921102991809907 15.217154169699052 20.89847716019997 2 14.592532726301572 21.129515334729728 41.77976268712229 22.498189251846405 3 18.7577453572595 26.72288062419191 31.086676419665267 23.432697598883326 4 11.50545005597113 17.326447561034254 39.20964962408201 31.958452758912603 5 12.116215471204175 39.45269450832583 34.26284239442518 14.168247626044817 6 29.081940331123647 38.07547446095859 18.16971553859989 14.672869669317883 7 26.556315965364657 35.48725652262843 20.812222740090736 17.144204771916176 8 25.89609636162325 35.23994551684937 19.943079974759346 18.92087814676804 9 26.859966509648036 16.795534470693706 21.191478516319282 35.15302050333897 10-11 24.120965341657783 28.132965738503696 27.251325032173863 20.49474388766466 12-13 25.627238991476776 25.91262263713827 28.183144088280017 20.276994283104933 14-15 22.51263325241382 26.587578487128376 27.94383494767152 22.955953312786278 16-17 20.161227447899943 30.36601168015957 28.27016104580993 21.202599826130548 18-19 20.049272468197994 28.01373995415358 32.20243070430399 19.734556873344438 20-21 21.528325162725263 26.73782476944635 32.14464840306409 19.589201664764307 22-23 20.863227343299695 27.064687848282272 31.903040171570364 20.169044636847673 24-25 20.397714985641827 27.1464477839127 32.44994755732233 20.005889673123143 26-27 20.364157312695404 27.25281191958272 32.34744883945185 20.03558192827003 28-29 20.029868548206352 27.549292326678305 32.47693207979329 19.943907045322057 30-31 20.21683186937217 27.452701987099182 32.26386343230592 20.06660271122273 32-33 19.998105287338205 27.466738431004657 32.1013788645641 20.433777417093047 34-35 20.476255412755435 27.45354028297226 31.94984384885195 20.120360455420354 36-37 20.447366649036205 27.248058344251085 32.05614477430076 20.24843023241194 38-39 20.325245958804153 27.626347705599937 31.940402682013424 20.108003653582486 40-41 20.435442839919922 27.66200583105041 31.905317375943493 19.99723395308617 42-43 20.23162687323281 27.75697145362391 31.536451180334712 20.47495049280857 44-45 20.46367711701401 27.97885752157892 31.06621036972793 20.491254991679135 46-47 20.604323525968454 28.081404267197023 30.496871033258945 20.81740117357558 48-49 20.720486348823837 28.32172658750495 30.20371278012533 20.75407428354588 50-51 20.755060766647077 28.66639223935204 29.922553929019458 20.655993064981427 52-53 20.902268668229983 28.834893386288492 29.669104802578072 20.59373314290346 54-55 20.637640387430608 28.670625024275186 29.778076577985036 20.913658010309167 56-57 20.854078468993624 29.129411094231294 29.30504750033105 20.711462936444036 58-59 20.958618653133165 28.703691028514612 29.48160101110139 20.856089307250837 60-61 20.876786301499223 28.663578685273155 29.90903409175043 20.55060092147719 62-63 20.868478198913884 28.542166574089496 29.992545395412566 20.596809831584054 64-65 20.740235579283038 28.670955903409194 29.93850554305017 20.650302974257595 66-67 20.682650192346 28.880314101340876 29.579822682383654 20.857213023929468 68-69 20.798400918231465 28.901547727416236 29.522968918602515 20.777082435749783 70-71 20.758811888712284 28.916858304879796 29.446469866258834 20.877859940149087 72-73 20.82439749442616 28.800381088617073 29.376456322885993 20.998765094070777 74-75 20.60297843080992 29.195618044928757 29.24121567988672 20.9601878443746 76-77 20.733098989040958 28.963725068319373 29.313872325205796 20.989303617433876 78-79 20.736030161945525 29.069715127555888 29.151284501064538 21.04297020943405 80-81 20.65501368325814 29.278567887905137 29.06528565512825 21.001132773708473 82-83 20.788271076282992 29.230380192492273 28.89398674712093 21.08736198410381 84-85 20.725962173835175 28.99626912899124 28.970340511481098 21.30742818569249 86-87 20.76072728288734 29.077205702590753 29.064632621557244 21.09743439296466 88-89 20.91684996177626 28.864173853176144 28.89381960184726 21.325156583200332 90-91 20.586050925087683 28.968086319163632 29.173428513310217 21.272434242438468 92-93 20.795066959227643 29.067421371909063 29.152194388942736 20.985317279920558 94-95 20.577884736552544 28.996697075035154 29.028214707725645 21.39720348068665 96-97 20.58306354912717 28.91025992036663 28.942807238300006 21.56386929220619 98-99 20.53855064397756 29.016876017347077 28.81548809469926 21.629085243976103 100-101 20.735209152160138 28.42853981831125 29.4878507242182 21.34840030531041 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2974.0 1 1777.5 2 670.5 3 760.5 4 711.0 5 785.5 6 1323.0 7 2782.0 8 4543.0 9 5304.0 10 4695.5 11 4080.5 12 4254.0 13 4414.5 14 4590.5 15 4742.0 16 4868.5 17 5104.0 18 5291.0 19 5557.5 20 6014.5 21 6828.0 22 7932.5 23 9298.5 24 10795.0 25 12845.0 26 15893.0 27 19279.0 28 22000.5 29 24505.5 30 27612.5 31 31219.5 32 35319.0 33 40098.5 34 45047.5 35 48544.5 36 52630.0 37 57305.5 38 59285.0 39 60045.0 40 61069.5 41 62200.0 42 63272.0 43 64619.5 44 66343.5 45 66820.0 46 65465.5 47 66192.5 48 74340.5 49 72030.5 50 59935.0 51 54074.0 52 49018.0 53 44842.5 54 41398.5 55 37152.5 56 32514.5 57 28042.0 58 24897.5 59 22021.5 60 18692.0 61 15437.5 62 11980.5 63 8499.5 64 5733.0 65 4162.5 66 2975.0 67 2216.5 68 1643.5 69 1095.5 70 821.0 71 676.0 72 556.0 73 482.0 74 384.0 75 298.5 76 227.5 77 139.5 78 92.5 79 75.0 80 59.5 81 42.0 82 26.0 83 20.5 84 12.5 85 5.5 86 4.5 87 5.0 88 4.0 89 2.5 90 1.0 91 2.5 92 3.5 93 2.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29143184789544374 2 0.2753682359923073 3 0.2623723277512282 4 0.2482051144755454 5 0.2454720693948034 6 0.2025242181260012 7 0.19443663574421377 8 0.2060939096600315 9 0.20637279181112764 10-11 0.20997037156026752 12-13 0.20645645645645644 14-15 0.20333297636417996 16-17 0.2007114841438764 18-19 0.20288676492242613 20-21 0.1912852674368276 22-23 0.1832534614852594 24-25 0.1840064432932189 26-27 0.19412986537800803 28-29 0.2063170153809084 30-31 0.201101919155411 32-33 0.20539670428229123 34-35 0.19967962018482077 36-37 0.18715781160060507 38-39 0.189388868809374 40-41 0.1848709779616169 42-43 0.19440874752910414 44-45 0.18930520416404517 46-47 0.1887195516467433 48-49 0.1973927865458326 50-51 0.2058986921542642 52-53 0.21959180577308365 54-55 0.19485495897085792 56-57 0.17262805152849728 58-59 0.17516587910347195 60-61 0.18102240427649044 62-63 0.1881896755596607 64-65 0.17840091205618697 66-67 0.18174749786934036 68-69 0.1865721590833032 70-71 0.18174749786934036 72-73 0.18194271537510764 74-75 0.18436899008964389 76-77 0.19421353002333686 78-79 0.20065570771365718 80-81 0.19293067212829473 82-83 0.19240079604121207 84-85 0.18648849443797438 86-87 0.18598650656600138 88-89 0.1900860741871143 90-91 0.190755391349745 92-93 0.18894265736762023 94-95 0.18950042166981246 96-97 0.17681128379493907 98-99 0.18356023185146514 100-101 0.1794885524454618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1792872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.80733811455182 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32351464700096 35.52434409541366 2 14.830485930080261 14.773340542452221 3 5.697401381798552 8.513171909926655 4 2.803096088329067 5.584590185559338 5 1.5195046123203544 3.7841239996230436 6 0.9399527870754407 2.8089927766549083 7 0.6067188415091586 2.1153335335661083 8 0.4239380972241188 1.6892182518465142 9 0.3004161812775 1.3466637284373841 >10 1.406213861781921 12.349451549858085 >50 0.09662316409111764 3.326024107433776 >100 0.047548024422915114 4.185117712551785 >500 0.0031321640604835095 1.035983001122 >1k 0.0011186300216012534 1.0121922760774105 >5k 1.1186300216012533E-4 0.455868178723255 >10k+ 2.2372600432025067E-4 1.4955841507538907 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15016 0.8375388761718628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11827 0.6596678402027585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8182 0.45636275205368815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2443 0.13626181902556345 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2380 0.13274790392175237 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2335 0.1302379645618873 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2217 0.1236563457960189 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1925 0.10736962817200557 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1915 0.10681186386981335 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.577643021922368E-5 0.0 0.0 0.0 0.0 8 5.577643021922368E-5 0.0 0.0 0.0 0.0 9 5.577643021922368E-5 0.0 0.0 0.0 0.0 10-11 5.577643021922368E-5 0.0 0.0 0.0 0.0 12-13 5.577643021922368E-5 0.0 0.0 0.0 0.0 14-15 1.1155286043844736E-4 0.0 0.0 2.788821510961184E-5 0.0 16-17 1.394410755480592E-4 0.0 0.0 5.577643021922368E-5 0.0 18-19 1.952175057672829E-4 0.0 0.0 5.577643021922368E-5 0.0 20-21 2.2310572087689472E-4 0.0 0.0 8.366464532883551E-5 0.0 22-23 2.2310572087689472E-4 0.0 0.0 2.5099393598650654E-4 0.0 24-25 3.904350115345658E-4 5.577643021922368E-5 0.0 6.135407324114605E-4 0.0 26-27 3.904350115345658E-4 5.577643021922368E-5 0.0 8.366464532883552E-4 0.0 28-29 4.4621144175378943E-4 5.577643021922368E-5 0.0 0.0019800632727824405 0.0 30-31 4.4621144175378943E-4 5.577643021922368E-5 0.0 0.0044621144175378945 0.0 32-33 4.4621144175378943E-4 5.577643021922368E-5 0.0 0.006888389132074124 0.0 34-35 4.4621144175378943E-4 5.577643021922368E-5 0.0 0.009286775631500743 0.0 36-37 5.856525173018486E-4 5.577643021922368E-5 0.0 0.01333056682239446 0.0 38-39 6.135407324114605E-4 5.577643021922368E-5 0.0 0.020079514878920526 0.0 40-41 6.135407324114605E-4 5.577643021922368E-5 0.0 0.0332148641955477 0.0 42-43 6.693171626306842E-4 5.577643021922368E-5 0.0 0.044342262024282826 0.0 44-45 6.693171626306842E-4 5.577643021922368E-5 0.0 0.05672462953295049 0.0 46-47 7.250935928499078E-4 5.577643021922368E-5 0.0 0.07995551271925715 0.0 48-49 7.250935928499078E-4 5.577643021922368E-5 0.0 0.09735775894765494 0.0 50-51 7.250935928499078E-4 5.577643021922368E-5 0.0 0.11810659098920614 0.0 52-53 7.250935928499078E-4 5.577643021922368E-5 0.0 0.14429362497713166 0.0 54-55 7.250935928499078E-4 5.577643021922368E-5 0.0 0.18035308711385978 0.0 56-57 7.808700230691316E-4 8.366464532883551E-5 0.0 0.2601133823273496 0.0 58-59 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.3229455309693051 0.0 60-61 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.3751801578696081 0.0 62-63 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.4347772735588486 0.0 64-65 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.49632656430576194 0.0 66-67 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.5929034532303477 0.0 68-69 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.7200737141301777 0.0 70-71 7.808700230691316E-4 1.1155286043844736E-4 0.0 0.7878141886314249 0.0 72-73 8.087582381787434E-4 1.1155286043844736E-4 0.0 0.8722039275531104 0.0 74-75 8.366464532883552E-4 1.1155286043844736E-4 0.0 1.0169716521871053 0.0 76-77 8.366464532883552E-4 1.1155286043844736E-4 0.0 1.1743727382657545 0.0 78-79 8.366464532883552E-4 1.1155286043844736E-4 0.0 1.3376861259476416 0.0 80-81 9.481993137268026E-4 1.1155286043844736E-4 0.0 1.4918242908584662 0.0 82-83 9.481993137268026E-4 1.1155286043844736E-4 0.0 1.7027986381626796 0.0 84-85 9.760875288364145E-4 1.1155286043844736E-4 0.0 2.006250306770366 0.0 86-87 0.0010039757439460264 1.1155286043844736E-4 0.0 2.246088956713028 0.0 88-89 0.0010039757439460264 1.1155286043844736E-4 0.0 2.5749746775006805 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16580 0.0 30.221962 1 GGTATCA 11230 0.0 30.014637 1 GTCGGGA 345 0.0 26.176039 2 GTACATG 12560 0.0 25.208744 1 ATCAACG 20000 0.0 24.853416 3 TATCAAC 20065 0.0 24.82513 2 TCAACGC 20285 0.0 24.55383 4 CAACGCA 20370 0.0 24.45274 5 TACATGG 12775 0.0 24.36968 2 GAGTACT 10585 0.0 24.006834 12-13 AACGCAG 20820 0.0 23.957819 6 ACATGGG 12750 0.0 23.44363 3 GTGTAGC 975 0.0 23.404707 1 AGTACTT 11045 0.0 22.53396 12-13 GTACTTT 11555 0.0 21.990932 14-15 CATGGGA 9420 0.0 21.896353 4 ACGCAGA 23005 0.0 21.62037 7 CGCAGAG 22940 0.0 21.619507 8 CGGGAGT 430 0.0 20.999943 4 ATGGGAT 3660 0.0 20.77764 5 >>END_MODULE