FastQCFastQC Report
Thu 26 May 2016
SRR937585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792872
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193541.079497030462855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163240.9104944468986074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114620.6393094431727419No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.20157601881227435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.1710105350521398No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27490.15332940667264588No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19630.1094891325203361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130750.032.741351
GTATCAA188600.029.2233351
GAGTACT142050.023.40783512-13
GTGTAGC9950.023.3984181
ATCAACG235800.023.267123
TCAACGC237000.023.1493134
TATCAAC237850.023.0865552
AGTACTT146050.023.02693612-13
CAACGCA238700.023.0242445
ACCGTCC1050.00219637922.6196148
TACATGG117600.022.6196122
AACGCAG243450.022.6121446
GTACATG118600.022.5947691
GTACTTT151300.021.81795314-15
ACATGGG118650.021.4986423
ACGCAGA268100.020.4639557
CGCAGAG267200.020.4617778
TACTTTT166800.020.13221414-15
AGAGTAC236500.019.76370610-11
GCAGAGT277350.019.5074279
Produced by FastQC (version 0.11.5)