##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937585_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792872 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28871776680098 33.0 31.0 34.0 31.0 34.0 2 32.47700895546364 34.0 31.0 34.0 31.0 34.0 3 32.52188611345372 34.0 31.0 34.0 31.0 34.0 4 36.130761705241646 37.0 35.0 37.0 35.0 37.0 5 36.06878739809646 37.0 35.0 37.0 35.0 37.0 6 36.13285443690347 37.0 35.0 37.0 35.0 37.0 7 36.08830245550156 37.0 35.0 37.0 35.0 37.0 8 36.06461364782316 37.0 35.0 37.0 35.0 37.0 9 37.74666178065138 39.0 37.0 39.0 35.0 39.0 10-11 37.68336361993494 39.0 37.0 39.0 35.0 39.0 12-13 37.57650016286718 39.0 37.0 39.0 35.0 39.0 14-15 38.755365413704936 40.0 38.0 41.0 35.0 41.0 16-17 38.73686827615134 40.0 38.0 41.0 34.5 41.0 18-19 38.79422931475309 40.0 38.0 41.0 35.0 41.0 20-21 38.736165772012725 40.0 38.0 41.0 34.5 41.0 22-23 38.68302589364997 40.0 38.0 41.0 34.5 41.0 24-25 38.68656016715081 40.0 38.0 41.0 34.0 41.0 26-27 38.50781427787371 40.0 38.0 41.0 34.0 41.0 28-29 38.45148426658456 40.0 38.0 41.0 34.0 41.0 30-31 38.37336491394812 40.0 38.0 41.0 34.0 41.0 32-33 38.161356192745494 40.0 37.0 41.0 34.0 41.0 34-35 37.97726301710328 40.0 37.0 41.0 33.5 41.0 36-37 37.782298736329196 40.0 37.0 41.0 33.0 41.0 38-39 37.61717624013315 40.0 37.0 41.0 33.0 41.0 40-41 37.40212630907281 40.0 36.0 41.0 33.0 41.0 42-43 37.11314611416766 40.0 36.0 41.0 32.5 41.0 44-45 36.68959886706915 40.0 36.0 41.0 31.0 41.0 46-47 36.160921973236235 39.5 35.5 41.0 30.0 41.0 48-49 35.6630116371944 39.0 35.0 41.0 27.5 41.0 50-51 35.362042298613616 39.0 35.0 41.0 26.5 41.0 52-53 35.09580745306971 39.0 35.0 40.5 25.5 41.0 54-55 34.873635708516844 39.0 35.0 40.0 24.0 41.0 56-57 34.61483948658912 38.5 35.0 40.0 23.5 41.0 58-59 34.3780002141815 38.0 34.0 40.0 23.0 41.0 60-61 34.054964883159535 38.0 34.0 40.0 22.0 41.0 62-63 33.674760105573625 37.5 34.0 40.0 20.5 41.0 64-65 33.298963060385795 37.0 33.0 40.0 19.0 41.0 66-67 32.90264586652031 36.5 33.0 39.0 17.5 41.0 68-69 32.45351313423379 36.0 32.0 39.0 13.5 40.0 70-71 32.04436903471078 35.5 32.0 38.5 10.0 40.0 72-73 31.577100596138486 35.0 31.0 38.0 7.5 40.0 74-75 31.106003105631636 35.0 31.0 37.0 7.0 39.0 76-77 29.82408113908857 33.5 29.5 36.0 2.0 38.5 78-79 30.407990364063913 34.0 30.5 36.0 2.0 39.0 80-81 30.220894743183003 34.0 31.0 36.0 2.0 38.0 82-83 29.82566351641389 34.0 30.0 35.5 2.0 37.0 84-85 29.415124169488955 34.0 30.0 35.0 2.0 37.0 86-87 29.080067623343997 34.0 29.0 35.0 2.0 36.0 88-89 28.665664643097777 34.0 29.0 35.0 2.0 36.0 90-91 28.178869991834333 34.0 29.0 35.0 2.0 35.5 92-93 27.74713811136545 34.0 27.5 35.0 2.0 35.0 94-95 27.27562871192143 33.0 26.5 35.0 2.0 35.0 96-97 26.782759449642807 33.0 25.5 35.0 2.0 35.0 98-99 26.019800353845675 33.0 24.5 34.5 2.0 35.0 100-101 23.651291057030285 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2748688627282547 1101 2 0.21873643810164367 1101 3 0.21110529513033072 1101 4 0.179487438367552 1101 5 0.13601529533364953 1101 6 0.168713610071066 1101 7 0.13227980600806433 1101 8 0.11859090573360476 1101 9 0.14153414101083683 1101 10-11 0.19073612257515293 1101 12-13 0.2103949790080435 1101 14-15 0.2259589645745521 1101 16-17 0.34731603771647457 1101 18-19 0.3215049604153606 1101 20-21 0.2596045403705034 1101 22-23 0.2540551542529954 1101 24-25 0.15042323340362174 1101 26-27 0.0463711393329973 1101 28-29 0.23911155560998765 1101 30-31 0.3524680158825291 1101 32-33 0.2888230565010872 1101 34-35 0.3432011385432787 1101 36-37 0.2450905812489168 1101 38-39 0.3618144682565756 1101 40-41 0.41220429968106487 1101 42-43 0.43214134722563813 1101 44-45 0.4045375928899446 1101 46-47 0.4541958890946134 1101 48-49 0.24720858663047096 1101 50-51 0.3792107000474303 1101 52-53 0.5486644294494525 1101 54-55 0.5859338046100504 1101 56-57 0.4534287638172856 1101 58-59 0.4647880679827807 1101 60-61 0.5638175609755649 1101 62-63 0.45062985243064446 1101 64-65 0.3364454943938924 1101 66-67 0.36931703553462825 1101 68-69 0.4480001201141164 1101 70-71 0.42755907894911616 1101 72-73 0.35857230163296805 1101 74-75 0.4347423809422537 1101 76-77 0.40389276148525965 1101 78-79 0.49519891865121934 1101 80-81 0.5416836510433995 1101 82-83 0.4014723417298782 1101 84-85 0.5184146519417894 1101 86-87 0.45968937477269733 1101 88-89 0.6773156512339575 1101 90-91 0.6918589479281394 1101 92-93 0.8714096592859839 1101 94-95 0.751114786153817 1101 96-97 0.8975206636260218 1101 98-99 0.9356646345901609 1101 100-101 1.3218419304694429 1102 1 0.2428165166107874 1102 2 0.26567712408284194 1102 3 0.3027505691174781 1102 4 0.14491525978854014 1102 5 0.1508698572823448 1102 6 0.19047527310258516 1102 7 0.17704615934435708 1102 8 0.20299129948758576 1102 9 0.2547393756225844 1102 10-11 0.2727064366132552 1102 12-13 0.29813649110053575 1102 14-15 0.2675347525650622 1102 16-17 0.39536560439407964 1102 18-19 0.38335859588409704 1102 20-21 0.3849925038517483 1102 22-23 0.3396860291975017 1102 24-25 0.3872609449981397 1102 26-27 0.41426040567667854 1102 28-29 0.47089946890691436 1102 30-31 0.4010030194533485 1102 32-33 0.5359316669741716 1102 34-35 0.4369174812636558 1102 36-37 0.5118671065630522 1102 38-39 0.536366187069909 1102 40-41 0.5205710892350055 1102 42-43 0.5707330976937044 1102 44-45 0.59581517014486 1102 46-47 0.5511949626146588 1102 48-49 0.5719967508490384 1102 50-51 0.7510770746155586 1102 52-53 0.825429566780798 1102 54-55 0.7203583254461989 1102 56-57 0.652186219085678 1102 58-59 0.7488193173795992 1102 60-61 0.7943223960428369 1102 62-63 0.8291635048848391 1102 64-65 0.9545128186039733 1102 66-67 0.8417349552010904 1102 68-69 0.9516013283983895 1102 70-71 0.7917100951818128 1102 72-73 0.9008400543400228 1102 74-75 0.8975948776127183 1102 76-77 0.9290061587714469 1102 78-79 0.9384380276863276 1102 80-81 0.9508847550986808 1102 82-83 0.9957185151746444 1102 84-85 1.0282372696336992 1102 86-87 1.0646006836186501 1102 88-89 1.1993480201273954 1102 90-91 1.1498474248336947 1102 92-93 1.25718915705664 1102 94-95 1.3624472766477496 1102 96-97 1.4086717184620525 1102 98-99 1.4458211710992614 1102 100-101 1.7734956006811835 1103 1 0.17971094944844168 1103 2 0.17969826209439788 1103 3 0.1763563206229506 1103 4 0.1389609768832898 1103 5 0.1325953089230012 1103 6 0.12867485525816846 1103 7 0.11938854597333659 1103 8 0.07518375370904806 1103 9 0.14923980575653673 1103 10-11 0.18400851354578407 1103 12-13 0.2317698940746027 1103 14-15 0.2906329460024466 1103 16-17 0.2203926413856081 1103 18-19 0.19158951637076882 1103 20-21 0.2679002093943339 1103 22-23 0.2633612166130135 1103 24-25 0.20917565557943618 1103 26-27 0.17120215324949584 1103 28-29 0.1905369274297044 1103 30-31 0.28738006258963367 1103 32-33 0.41180176651946 1103 34-35 0.44383342079911614 1103 36-37 0.41797869560078027 1103 38-39 0.4246063247127765 1103 40-41 0.504469946307637 1103 42-43 0.7389191613535289 1103 44-45 0.7982171610505944 1103 46-47 0.8199156213340899 1103 48-49 0.717479115356852 1103 50-51 0.8750318491024629 1103 52-53 0.8428887040549782 1103 54-55 0.7166739208744914 1103 56-57 0.8405406494654244 1103 58-59 0.6862188503770241 1103 60-61 0.8987798127811928 1103 62-63 0.770621044518613 1103 64-65 0.9174149938528373 1103 66-67 0.9517675224863282 1103 68-69 0.8650467013859782 1103 70-71 0.777584496066666 1103 72-73 0.7870886717665968 1103 74-75 0.8762706795053106 1103 76-77 0.6620558085195078 1103 78-79 0.9506083607329074 1103 80-81 0.8925770249770153 1103 82-83 1.0258242052324036 1103 84-85 1.1033441886456181 1103 86-87 1.1931984469220787 1103 88-89 1.2905560820666828 1103 90-91 1.3816650480363464 1103 92-93 1.3173894765847223 1103 94-95 1.3815550559275778 1103 96-97 1.5066722100437175 1103 98-99 1.7289543897302053 1103 100-101 2.207057724313973 1104 1 0.19212310599003501 1104 2 0.23247823591385242 1104 3 0.20087781639601587 1104 4 0.13593925219168312 1104 5 0.1409034397225355 1104 6 0.15417779895710737 1104 7 0.1704790365038491 1104 8 0.15542466628795637 1104 9 0.13576134022006414 1104 10-11 0.17927318851369733 1104 12-13 0.21575849328661434 1104 14-15 0.26509273049659754 1104 16-17 0.28138672819409294 1104 18-19 0.23754539635297078 1104 20-21 0.19958627502624893 1104 22-23 0.20873145204443944 1104 24-25 0.16745818619325092 1104 26-27 0.20694336567945015 1104 28-29 0.22851264271952942 1104 30-31 0.3113094654024309 1104 32-33 0.32747410993595594 1104 34-35 0.38432943245783946 1104 36-37 0.4511217148750717 1104 38-39 0.4954720236881158 1104 40-41 0.527864389068327 1104 42-43 0.5498241956268259 1104 44-45 0.6164628067620797 1104 46-47 0.7698947929713995 1104 48-49 0.8497396053070005 1104 50-51 0.764646556634851 1104 52-53 0.8094560222259517 1104 54-55 0.6819516861136918 1104 56-57 0.8263584045161423 1104 58-59 0.7185703918693136 1104 60-61 0.7463012376217435 1104 62-63 0.6853663645854269 1104 64-65 0.7721867523985964 1104 66-67 0.8321517187243259 1104 68-69 0.8547435616553898 1104 70-71 0.9078252151932844 1104 72-73 0.6582676344754432 1104 74-75 0.915141949906463 1104 76-77 0.8455324040172307 1104 78-79 0.8381894978454376 1104 80-81 0.8355073112219102 1104 82-83 0.8408804676052881 1104 84-85 0.7044448031790438 1104 86-87 0.7382941090770103 1104 88-89 0.6538455447207205 1104 90-91 0.7404468145136462 1104 92-93 0.7834747116614906 1104 94-95 0.7843080109112321 1104 96-97 1.0132651928845 1104 98-99 1.278515704431932 1104 100-101 1.363652706161698 1105 1 0.1329447491614033 1105 2 0.1658709336538564 1105 3 0.17758636571915787 1105 4 0.09444156102547652 1105 5 0.06915316879145195 1105 6 0.06800405760616712 1105 7 0.12140950925019212 1105 8 0.20327223206898282 1105 9 0.12836223210959474 1105 10-11 0.1807539682149084 1105 12-13 0.07616075511977272 1105 14-15 0.1895922641858121 1105 16-17 0.2242605768225232 1105 18-19 0.25567239001295405 1105 20-21 0.23675974455645132 1105 22-23 0.13680927434705126 1105 24-25 0.10884345361913716 1105 26-27 0.14810704309366685 1105 28-29 0.16323446992421253 1105 30-31 0.1551917059826664 1105 32-33 0.1272320170312966 1105 34-35 0.045977361378220394 1105 36-37 0.0743825617677345 1105 38-39 0.1599846091521968 1105 40-41 0.21270275975351893 1105 42-43 0.26909784574126405 1105 44-45 0.3135604840224602 1105 46-47 0.13328831465943836 1105 48-49 0.2682366364935689 1105 50-51 0.30019567146556625 1105 52-53 0.32705347187410183 1105 54-55 0.4540368467548177 1105 56-57 0.3114016946364586 1105 58-59 0.4600274057338112 1105 60-61 0.59115127497315 1105 62-63 0.4001901478317933 1105 64-65 0.4476197973136209 1105 66-67 0.5138818070345863 1105 68-69 0.4814421332689278 1105 70-71 0.5605205791579593 1105 72-73 0.5079005396204082 1105 74-75 0.5121604624585316 1105 76-77 0.49363199935198665 1105 78-79 0.6270225656996118 1105 80-81 0.7137674833531449 1105 82-83 0.6051238875863874 1105 84-85 0.7275380683482418 1105 86-87 0.8768182511650693 1105 88-89 0.8425730322567979 1105 90-91 0.9790545011002365 1105 92-93 0.9318658367830643 1105 94-95 1.1011888447575942 1105 96-97 1.1491758320245502 1105 98-99 1.1905310566916185 1105 100-101 1.083877455097067 1106 1 -0.3534050554067676 1106 2 -0.2843627748398845 1106 3 -0.28244028072957406 1106 4 -0.16002289688123028 1106 5 -0.1710426310804678 1106 6 -0.2388437852828389 1106 7 -0.2857441053184928 1106 8 -0.2777415107265355 1106 9 -0.552802850737308 1106 10-11 -0.48691917849513544 1106 12-13 -0.5577532548763244 1106 14-15 -0.5989248830247789 1106 16-17 -0.576652616113968 1106 18-19 -0.5476475219507151 1106 20-21 -0.6122385109108563 1106 22-23 -0.5225170563191881 1106 24-25 -0.5436421006196284 1106 26-27 -0.5671540111340647 1106 28-29 -0.6292827361735576 1106 30-31 -0.6403362543862627 1106 32-33 -0.8359735065861429 1106 34-35 -1.1544291073784478 1106 36-37 -0.8232559151153609 1106 38-39 -0.7058774623515589 1106 40-41 -0.7972883335605729 1106 42-43 -0.8004878093418029 1106 44-45 -0.8471513147600476 1106 46-47 -0.7339945572683035 1106 48-49 -0.7905527903133418 1106 50-51 -0.8917238297600321 1106 52-53 -0.9370315317695415 1106 54-55 -1.0166120163545784 1106 56-57 -1.2141151507773387 1106 58-59 -1.1378491225725185 1106 60-61 -1.2121478792597387 1106 62-63 -1.3224949603365914 1106 64-65 -1.6546606087134883 1106 66-67 -1.8223819486425015 1106 68-69 -1.81663181430676 1106 70-71 -1.7037680269088895 1106 72-73 -1.6286352748260882 1106 74-75 -1.824658746539427 1106 76-77 -1.6891458561241528 1106 78-79 -1.805954843706143 1106 80-81 -1.7635041264015214 1106 82-83 -1.9103713903979056 1106 84-85 -1.835830425538532 1106 86-87 -1.7433578215017107 1106 88-89 -1.9380931289615795 1106 90-91 -2.045125310902442 1106 92-93 -2.190382328513248 1106 94-95 -2.2712939123401483 1106 96-97 -2.5431769316243944 1106 98-99 -2.553313687259564 1106 100-101 -1.9532439923498046 1107 1 0.07711994950901158 1107 2 0.13003790098833434 1107 3 0.1060371394709918 1107 4 0.08634824059300428 1107 5 0.0784145943825223 1107 6 0.07125212697601313 1107 7 0.011437079274372763 1107 8 0.026726092486562436 1107 9 0.10807936726099143 1107 10-11 0.04282600222336441 1107 12-13 0.09458862098393439 1107 14-15 0.10581407104366036 1107 16-17 0.1860913338397907 1107 18-19 0.19517231302484817 1107 20-21 0.12952417725414023 1107 22-23 0.18665585520411554 1107 24-25 0.12150992431610064 1107 26-27 0.17514686350999398 1107 28-29 0.10686489733529214 1107 30-31 0.13695038271291793 1107 32-33 0.21676774879389882 1107 34-35 0.20155117125503352 1107 36-37 0.17163881444410833 1107 38-39 0.14276512793486518 1107 40-41 0.062497120110961646 1107 42-43 -0.022740472964329683 1107 44-45 0.020630905423040247 1107 46-47 -0.14623462193807768 1107 48-49 -0.1204187170646378 1107 50-51 -0.2067294009732379 1107 52-53 -0.21652407799604134 1107 54-55 -0.12188784602837899 1107 56-57 -0.08396476443373047 1107 58-59 -0.010193911485444573 1107 60-61 -0.18750636680280053 1107 62-63 -0.005622268020495369 1107 64-65 -0.011071873488127437 1107 66-67 0.1840576455692613 1107 68-69 0.13014700488734832 1107 70-71 0.03876108541113865 1107 72-73 0.047795944359279474 1107 74-75 0.11686099303366504 1107 76-77 0.040288501875455296 1107 78-79 0.1911385123490028 1107 80-81 0.22756649327761025 1107 82-83 0.14915451028156212 1107 84-85 0.27489689088452707 1107 86-87 0.3381630832924465 1107 88-89 0.3571384951381056 1107 90-91 0.403334776634793 1107 92-93 0.35212187720653176 1107 94-95 0.622950623865453 1107 96-97 0.42052788874949343 1107 98-99 0.6411532605166812 1107 100-101 1.0967094716339396 1108 1 0.10621934880166606 1108 2 0.06474802225785226 1108 3 0.07935437892792407 1108 4 0.09865761240616422 1108 5 0.03799890410326867 1108 6 0.08353308445936847 1108 7 0.07975848285133935 1108 8 0.1431217006540919 1108 9 0.10934778294330982 1108 10-11 0.08269343800910889 1108 12-13 0.15797871762391225 1108 14-15 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92-93 0.36023098592113456 2316 94-95 0.25196858101568864 2316 96-97 0.5506740712943703 2316 98-99 0.3501912597270156 2316 100-101 0.7062834095730359 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 13.0 5 28.0 6 76.0 7 193.0 8 504.0 9 910.0 10 1692.0 11 3097.0 12 4758.0 13 7482.0 14 9585.0 15 14037.0 16 20225.0 17 30422.0 18 27451.0 19 14071.0 20 10596.0 21 10057.0 22 10103.0 23 10774.0 24 11479.0 25 13669.0 26 16086.0 27 19778.0 28 24179.0 29 29601.0 30 37114.0 31 46788.0 32 59796.0 33 78146.0 34 109429.0 35 160280.0 36 244276.0 37 391692.0 38 342611.0 39 31864.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4288188535265 20.266391544571007 15.889751776334219 20.415037825568277 2 15.202646926272484 21.512690253403477 40.90866497998742 22.375997840336623 3 18.842951420960336 26.577468999460084 31.817274183544615 22.762305396034964 4 11.894491073540108 17.953428911824158 38.8349530808669 31.317126933768836 5 12.053277582048324 39.384869059598685 34.36145218115003 14.20040117720296 6 29.089304757952604 38.44340254072795 17.604324235082036 14.862968466237412 7 26.23567103507668 35.850635181987336 20.65535074450379 17.25834303843219 8 26.25814893645503 35.06457795090782 19.69053005457166 18.986743058065496 9 26.735037414829392 16.547639764578843 21.122645676880445 35.594677143711316 10-11 24.20950854271805 28.1390696045228 26.97579637587067 20.675625476888477 12-13 25.941829151020645 25.95159048364893 28.185931632741852 19.92064873258857 14-15 22.560422606856484 26.497401933880386 28.310498462801583 22.631676996461543 16-17 19.947241074655636 30.12320455671124 28.807773226421073 21.121781142212047 18-19 19.837305730693547 27.31614415306837 33.30784350472315 19.538706611514932 20-21 21.133903890303134 26.01906554191385 33.57070905387346 19.276321513909558 22-23 20.574184296526347 26.242512573633213 33.46017127025907 19.723131859581365 24-25 20.190961817397397 26.36956970982025 33.88737006528354 19.552098407498807 26-27 20.149867278577773 26.513439752171543 33.78704221706966 19.549650752181023 28-29 19.7920614668089 26.74397428657087 33.952770398878876 19.51119384774136 30-31 20.049590824107913 26.64607400862973 33.65789638077899 19.646438786483365 32-33 19.87699620413398 26.636738885079048 33.50675159931986 19.979513311467105 34-35 20.343309505642342 26.50956119566818 33.458411978099946 19.688717320589536 36-37 20.272529975731608 26.251194316142808 33.60076346990602 19.875512238219567 38-39 20.16978903123034 26.73793778920079 33.485351993895826 19.606921185673045 40-41 20.20867676575276 26.856905567809353 33.333565735579604 19.600851930858287 42-43 20.11781074410779 26.891761169650145 32.92544752239285 20.064980563849204 44-45 20.33075980884302 27.058596486531105 32.43441249570522 20.176231208920658 46-47 20.458683051550807 27.444318389712148 31.703741259833386 20.39325729890366 48-49 20.654235215899405 27.676710886220548 31.24782471922145 20.4212291786586 50-51 20.746517319697112 27.95219067507329 30.926998692600478 20.37429331262912 52-53 20.913238901951424 28.25063034355781 30.416485509418827 20.41964524507194 54-55 20.637390733973202 28.1283326417056 30.515396525797716 20.71888009852349 56-57 20.84936905702136 28.465696379886573 30.114140886800616 20.570793676291448 58-59 21.04048699516753 28.029385254496695 30.382174522219096 20.547953228116675 60-61 20.89265156687148 27.98961665975039 30.783485937646414 20.33424583573172 62-63 20.898563868474714 27.94507918022034 30.790848426435353 20.365508524869593 64-65 20.76467254773347 28.025313575090692 30.71390484094793 20.496109036227907 66-67 20.75789571146183 28.214339897103642 30.397457264099163 20.63030712733536 68-69 20.90397418220598 28.170220740800232 30.333927910079474 20.591877166914312 70-71 20.92413736173023 28.245825691962395 30.223295360739638 20.606741585567736 72-73 20.89953995600355 28.13681065909892 30.14066257936986 20.82298680552767 74-75 20.766568946360923 28.549416801645627 29.808513937414382 20.875500314579064 76-77 20.73087203102062 28.27031154482863 30.17800489940163 20.820811524749118 78-79 20.795851572226017 28.315797223672412 29.959110299006287 20.929240905095288 80-81 20.75376825562561 28.554548233225795 29.82315525034693 20.868528260801664 82-83 20.775394291326997 28.494030655343654 29.700839743684494 21.029735309644852 84-85 20.65470538118405 28.324541551453187 29.773310074503556 21.247442992859206 86-87 20.65618739095708 28.273379248490688 29.88144161992602 21.18899174062621 88-89 20.751397757341294 28.09305404959194 29.757199621612695 21.398348571454072 90-91 20.453173090198124 28.106019297788105 30.026036255520676 21.414771356493095 92-93 20.51709395273498 28.183561492547803 30.044254130487776 21.255090424229444 94-95 20.328640304494687 28.061763472238955 30.00769156972723 21.601904653539126 96-97 20.326046700489496 27.95001539429474 30.0009147334556 21.72302317176017 98-99 20.245858160941467 28.03845023295143 29.782476407845998 21.933215198261106 100-101 20.103919185247705 27.811477243377514 30.291000494800137 21.793603076574644 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1087.0 1 782.0 2 686.0 3 922.0 4 746.5 5 670.0 6 1408.0 7 3464.5 8 5734.0 9 6542.5 10 5625.5 11 4506.0 12 4490.5 13 4820.5 14 5082.0 15 5284.0 16 5477.5 17 5747.0 18 5977.5 19 6270.5 20 6702.0 21 7468.0 22 8570.5 23 9785.0 24 11228.5 25 13294.5 26 16099.5 27 19386.5 28 22009.5 29 24261.0 30 27512.0 31 31067.5 32 34836.5 33 39387.0 34 44122.0 35 47916.0 36 52113.0 37 56477.5 38 58648.5 39 59741.5 40 60889.0 41 61778.5 42 62950.5 43 64587.0 44 66096.5 45 66631.5 46 65355.5 47 66045.5 48 74838.5 49 72383.5 50 59979.5 51 54461.5 52 48898.5 53 44981.5 54 41725.5 55 36692.0 56 31888.0 57 27429.5 58 24384.5 59 21966.0 60 18527.0 61 14840.0 62 11403.0 63 8130.0 64 5471.0 65 3922.0 66 2850.5 67 2154.0 68 1531.5 69 989.5 70 719.5 71 592.5 72 512.0 73 479.5 74 400.0 75 317.0 76 234.0 77 149.5 78 95.0 79 74.0 80 49.0 81 20.5 82 14.0 83 13.5 84 8.5 85 3.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03893194829301813 2 0.0 3 0.0 4 0.0 5 0.008254911672445105 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0046852201384147895 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2310572087689474E-4 22-23 0.0018406221972343815 24-25 2.7888215109611843E-5 26-27 0.0010597521741652499 28-29 0.001924286842563217 30-31 0.0 32-33 2.7888215109611843E-5 34-35 0.0 36-37 2.788821510961184E-4 38-39 0.0 40-41 1.952175057672829E-4 42-43 0.018740880553659158 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.7888215109611843E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.952175057672829E-4 84-85 2.5099393598650654E-4 86-87 0.0 88-89 0.0 90-91 0.010011869224350652 92-93 0.0025099393598650654 94-95 0.0 96-97 0.0 98-99 5.019878719730131E-4 100-101 0.012772802520202225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1792872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.69062894658592 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02941775934852 33.39746977706544 2 15.25435352398772 14.549794274650873 3 5.98062187407878 8.556588559995792 4 2.9543850668328147 5.635859279506327 5 1.6671356044902816 3.9753372758694114 6 1.0101473292349363 2.8904736875956867 7 0.6569037238763877 2.1929706224313534 8 0.4512731029817687 1.721719848630239 9 0.3294571710954804 1.4140817730455784 >10 1.5016669405018475 12.66325412357158 >50 0.10688552210877081 3.5225724933446805 >100 0.052608915898558144 4.489572313382148 >500 0.0029224236159426337 0.9273270049392429 >1k 0.0018703511142032858 1.434976380133228 >5k 0.0 0.0 >10k+ 3.5069083391311604E-4 2.628002585838398 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19354 1.079497030462855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16324 0.9104944468986074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11462 0.6393094431727419 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3614 0.20157601881227435 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3066 0.1710105350521398 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2749 0.15332940667264588 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1963 0.1094891325203361 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 5.577643021922368E-5 0.0 0.0 2.788821510961184E-5 0.0 16-17 5.577643021922368E-5 0.0 0.0 5.577643021922368E-5 0.0 18-19 5.577643021922368E-5 0.0 0.0 5.577643021922368E-5 0.0 20-21 5.577643021922368E-5 0.0 0.0 8.366464532883551E-5 0.0 22-23 1.1155286043844736E-4 0.0 0.0 2.5099393598650654E-4 0.0 24-25 1.1155286043844736E-4 0.0 0.0 6.135407324114605E-4 0.0 26-27 1.1155286043844736E-4 0.0 0.0 8.366464532883552E-4 0.0 28-29 1.1155286043844736E-4 0.0 0.0 0.0020079514878920527 0.0 30-31 1.1155286043844736E-4 0.0 0.0 0.004517890847757118 0.0 32-33 1.1155286043844736E-4 0.0 0.0 0.00697205377740296 0.0 34-35 1.6732929065767105E-4 0.0 0.0 0.009398328491939192 0.0 36-37 2.2310572087689472E-4 0.0 0.0 0.013414231467723296 0.0 38-39 3.346585813153421E-4 0.0 0.0 0.020386285245126257 0.0 40-41 3.346585813153421E-4 0.0 0.0 0.03374474028263033 0.0 42-43 3.346585813153421E-4 0.0 0.0 0.044537479530050106 0.0 44-45 3.346585813153421E-4 0.0 0.0 0.057310282050252334 0.0 46-47 4.740996568634013E-4 0.0 0.0 0.08098737667831278 0.0 48-49 5.577643021922368E-4 0.0 0.0 0.09878005791824515 0.0 50-51 5.577643021922368E-4 0.0 0.0 0.11964044282023478 0.0 52-53 5.577643021922368E-4 0.0 0.0 0.1457717003779411 0.0 54-55 5.856525173018486E-4 0.0 0.0 0.18219370931109416 0.0 56-57 6.135407324114605E-4 0.0 0.0 0.2624281041814474 0.0 58-59 6.135407324114605E-4 0.0 0.0 0.3254833585442798 0.0 60-61 6.693171626306842E-4 0.0 0.0 0.3773275504330482 0.0 62-63 6.693171626306842E-4 0.0 0.0 0.43633901360498684 0.0 64-65 6.693171626306842E-4 0.0 0.0 0.497971968997229 0.0 66-67 7.250935928499078E-4 0.0 0.0 0.5954133925902128 0.0 68-69 7.250935928499078E-4 0.0 0.0 0.7227230945655909 0.0 70-71 7.808700230691316E-4 0.0 0.0 0.7905472337121668 0.0 72-73 8.645346683979671E-4 0.0 0.0 0.8752158547849485 0.0 74-75 8.924228835075789E-4 0.0 0.0 1.0215174312499722 0.0 76-77 8.924228835075789E-4 0.0 0.0 1.1808985806014038 0.0 78-79 8.924228835075789E-4 0.0 0.0 1.3447418443703731 0.0 80-81 0.0010039757439460264 0.0 0.0 1.4992146678625133 0.0 82-83 0.0010039757439460264 0.0 0.0 1.711053549835125 0.0 84-85 0.0010039757439460264 0.0 0.0 2.0179354689012934 0.0 86-87 0.0010039757439460264 0.0 0.0 2.259475299965642 0.0 88-89 0.0010039757439460264 0.0 0.0 2.5919307122873243 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13075 0.0 32.74135 1 GTATCAA 18860 0.0 29.223335 1 GAGTACT 14205 0.0 23.407835 12-13 GTGTAGC 995 0.0 23.398418 1 ATCAACG 23580 0.0 23.26712 3 TCAACGC 23700 0.0 23.149313 4 TATCAAC 23785 0.0 23.086555 2 AGTACTT 14605 0.0 23.026936 12-13 CAACGCA 23870 0.0 23.024244 5 ACCGTCC 105 0.002196379 22.619614 8 TACATGG 11760 0.0 22.619612 2 AACGCAG 24345 0.0 22.612144 6 GTACATG 11860 0.0 22.594769 1 GTACTTT 15130 0.0 21.817953 14-15 ACATGGG 11865 0.0 21.498642 3 ACGCAGA 26810 0.0 20.463955 7 CGCAGAG 26720 0.0 20.461777 8 TACTTTT 16680 0.0 20.132214 14-15 AGAGTAC 23650 0.0 19.763706 10-11 GCAGAGT 27735 0.0 19.507427 9 >>END_MODULE