FastQCFastQC Report
Thu 26 May 2016
SRR937584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525628
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132030.8654141114347665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102600.6725099434462398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.4685283699565032No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.14826681209311837No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20540.13463308224547532No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20070.13155238367413288No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20030.1312901965616782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.11287155191173734No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16600.10880765166868987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105950.030.0705721
GTATCAA148550.027.5932241
GTACATG106650.025.8603841
TACATGG106450.025.3612462
ACATGGG108100.023.8741443
GAGTACT92900.023.51969712-13
CATGGGA80800.022.766494
ATCAACG179250.022.5909293
TATCAAC181650.022.4240112
TCAACGC181150.022.3561864
CAACGCA182200.022.2005335
ACACCGA1503.0284937E-522.1719266
AACGCAG185100.021.920386
GTACTTT99950.021.69297414-15
TAGCCCT11500.021.4932464
AGTACTT96800.021.4925712-13
CATGGGG27300.020.545464
ATGGGAG27950.020.2370575
ACGCAGA205900.019.6361167
CGCAGAG205950.019.608288
Produced by FastQC (version 0.11.5)