##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937584_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525628 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86625311019462 33.0 31.0 34.0 30.0 34.0 2 31.948073842378353 33.0 31.0 34.0 30.0 34.0 3 31.975640195381835 33.0 31.0 34.0 30.0 34.0 4 35.60072901126618 37.0 35.0 37.0 33.0 37.0 5 35.627463575655405 37.0 35.0 37.0 33.0 37.0 6 35.70693773318266 37.0 35.0 37.0 35.0 37.0 7 35.63296360580692 37.0 35.0 37.0 33.0 37.0 8 35.64888491821073 37.0 35.0 37.0 33.0 37.0 9 37.21557876494139 39.0 37.0 39.0 34.0 39.0 10-11 37.14995038108897 39.0 37.0 39.0 34.0 39.0 12-13 37.059255926084205 39.0 37.0 39.0 33.0 39.0 14-15 38.15313824864253 40.0 38.0 41.0 33.0 41.0 16-17 38.093078063590866 40.0 38.0 41.0 33.0 41.0 18-19 38.09719046844971 40.0 38.0 41.0 33.5 41.0 20-21 38.14560823477283 40.0 38.0 41.0 33.5 41.0 22-23 38.142469199568964 40.0 38.0 41.0 34.0 41.0 24-25 38.07122083496108 40.0 38.0 41.0 33.0 41.0 26-27 37.97011034144627 40.0 38.0 41.0 33.0 41.0 28-29 37.823511367122265 40.0 38.0 41.0 33.0 41.0 30-31 37.550437590290684 40.0 37.0 41.0 33.0 41.0 32-33 37.36245008612847 40.0 36.0 41.0 32.5 41.0 34-35 37.302309278539724 40.0 36.0 41.0 32.5 41.0 36-37 37.12276059432575 40.0 36.0 41.0 32.0 41.0 38-39 36.94530383553527 40.0 36.0 41.0 31.0 41.0 40-41 36.68990835249484 39.0 35.5 41.0 30.5 41.0 42-43 36.38472124266204 39.0 35.0 41.0 30.0 41.0 44-45 36.03978525564554 39.0 35.0 41.0 29.5 41.0 46-47 35.53929791535027 39.0 35.0 40.5 27.5 41.0 48-49 35.08257943614039 39.0 35.0 40.0 24.5 41.0 50-51 34.172749844654135 38.0 34.0 39.5 23.5 40.5 52-53 34.26957587301754 38.0 34.0 39.5 23.5 40.5 54-55 34.54782718985231 38.0 35.0 40.0 23.0 41.0 56-57 34.335466771716305 38.0 34.0 40.0 22.5 41.0 58-59 34.0838926658399 38.0 34.0 40.0 21.5 41.0 60-61 33.753641451258105 38.0 34.0 40.0 19.5 41.0 62-63 33.40013587847103 37.5 33.0 40.0 18.0 41.0 64-65 32.99992068839848 37.0 33.0 40.0 14.5 41.0 66-67 32.50360081225567 36.0 32.0 39.0 8.0 41.0 68-69 32.05655998710039 36.0 31.5 39.0 7.5 40.0 70-71 31.60087681925083 35.0 31.0 38.5 6.0 40.0 72-73 31.133594821280155 35.0 31.0 38.0 2.0 40.0 74-75 30.600780793220892 35.0 30.0 37.0 2.0 39.0 76-77 30.062512617754784 34.5 29.5 36.5 2.0 39.0 78-79 29.541728389882724 34.0 29.0 36.0 2.0 39.0 80-81 29.005875613190106 34.0 29.0 36.0 2.0 37.5 82-83 28.461207122575097 34.0 27.5 35.0 2.0 37.0 84-85 27.915102829785504 34.0 26.5 35.0 2.0 36.5 86-87 27.34608731617406 33.0 26.0 35.0 2.0 36.0 88-89 26.798153612807315 33.0 25.0 35.0 2.0 36.0 90-91 26.219791456370753 32.0 24.5 35.0 2.0 35.0 92-93 25.57105434614467 32.0 21.5 34.0 2.0 35.0 94-95 24.805429632911824 31.5 18.5 34.0 2.0 35.0 96-97 23.937352355882297 31.0 4.5 34.0 2.0 35.0 98-99 22.774394216676676 31.0 2.0 34.0 2.0 35.0 100-101 19.619607794298478 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06998812557058898 1101 2 -0.02260803414177559 1101 3 0.05917753834997441 1101 4 -0.040928538902043954 1101 5 0.00199024659625735 1101 6 -0.011471969343055832 1101 7 -0.13929782653092815 1101 8 0.011081993048236427 1101 9 0.19712289598173527 1101 10-11 0.13890281738684962 1101 12-13 0.16345486060053815 1101 14-15 0.23603262221769228 1101 16-17 0.29497551130018707 1101 18-19 0.38712961765865117 1101 20-21 0.2710787429844643 1101 22-23 0.17190765977925082 1101 24-25 0.11132540918129763 1101 26-27 0.1655626427476733 1101 28-29 0.1350709952407101 1101 30-31 0.09476922090405537 1101 32-33 0.15330016771957133 1101 34-35 0.3260938537352587 1101 36-37 0.3757940655951515 1101 38-39 0.3099916578276094 1101 40-41 0.12954357979980813 1101 42-43 0.15307193099963712 1101 44-45 0.4512282422183347 1101 46-47 0.537699611389229 1101 48-49 0.7414892639430377 1101 50-51 0.7223117959489898 1101 52-53 0.6983012307394887 1101 54-55 0.7088666509205055 1101 56-57 0.8085031417713751 1101 58-59 0.7275956964469188 1101 60-61 0.745353252683401 1101 62-63 0.8889986344822276 1101 64-65 0.9282289539289721 1101 66-67 0.9772091064875355 1101 68-69 0.9998345673660154 1101 70-71 0.8713489713212645 1101 72-73 1.0094926477512622 1101 74-75 1.0858648845080552 1101 76-77 1.0072666383017967 1101 78-79 1.3598165836550393 1101 80-81 1.3097118527740896 1101 82-83 1.3134274673282214 1101 84-85 1.4013733093161527 1101 86-87 1.6595514085346998 1101 88-89 1.7841822638207638 1101 90-91 1.77816416429172 1101 92-93 1.7818810242271574 1101 94-95 2.2218923386070166 1101 96-97 2.2519312735916124 1101 98-99 2.5188211939688223 1101 100-101 3.035392189297138 1102 1 0.08452831429875118 1102 2 -0.0034762214938162117 1102 3 -0.05400767252486105 1102 4 -5.243048150944674E-4 1102 5 -0.10262808282850955 1102 6 -0.05654980574696111 1102 7 -0.06977264262837224 1102 8 -0.0390821165871742 1102 9 0.156044215895065 1102 10-11 0.2050060284667765 1102 12-13 0.11279776901065475 1102 14-15 0.18653795651031402 1102 16-17 0.23616796072854385 1102 18-19 0.13877509082545458 1102 20-21 0.12860612446385034 1102 22-23 0.1516210633027626 1102 24-25 0.25407953318099175 1102 26-27 0.19267929022583274 1102 28-29 0.04854418649853187 1102 30-31 0.179997853095891 1102 32-33 0.17115700787197596 1102 34-35 0.29492441661560775 1102 36-37 0.4272003563672513 1102 38-39 0.4404162308938382 1102 40-41 0.3431281505206272 1102 42-43 0.49916374291396437 1102 44-45 0.4163244806882105 1102 46-47 0.42440324336569546 1102 48-49 0.5429663479242066 1102 50-51 0.5397240908744649 1102 52-53 0.4347017788553913 1102 54-55 0.5415121993962728 1102 56-57 0.5203925659745678 1102 58-59 0.5287790476626597 1102 60-61 0.6848306862538038 1102 62-63 0.5955309788504124 1102 64-65 0.6992267477374625 1102 66-67 0.8277146908837381 1102 68-69 0.7210880087218641 1102 70-71 0.730062260630806 1102 72-73 0.6959041587409551 1102 74-75 0.5745654824501472 1102 76-77 0.672495487250071 1102 78-79 0.8830778843770908 1102 80-81 0.9397065255677362 1102 82-83 1.050845659140439 1102 84-85 1.162386829082255 1102 86-87 1.2725505701319086 1102 88-89 1.3097946771841826 1102 90-91 1.2528921696961817 1102 92-93 1.4296686218127128 1102 94-95 1.522212971402432 1102 96-97 1.753257100482692 1102 98-99 2.0136226520171405 1102 100-101 2.602103846819741 1103 1 0.07281201298969009 1103 2 0.04487133816596156 1103 3 0.1097460251654887 1103 4 0.11452207719960938 1103 5 0.038141139388677914 1103 6 0.1302448109119041 1103 7 0.12883861933130447 1103 8 0.1563303955674229 1103 9 0.10425730890453622 1103 10-11 0.14066087136144034 1103 12-13 0.2630160029392954 1103 14-15 0.3214272203588493 1103 16-17 0.42949534183873794 1103 18-19 0.3080290360975013 1103 20-21 0.2138275354771224 1103 22-23 0.13421323367774107 1103 24-25 0.07829952255934813 1103 26-27 0.04313438728726737 1103 28-29 0.13191735810700322 1103 30-31 0.11966617059226792 1103 32-33 0.3196638351118324 1103 34-35 0.08969786297276983 1103 36-37 0.2919365129586069 1103 38-39 0.29996842302522 1103 40-41 0.3885978045875689 1103 42-43 0.46586407613013137 1103 44-45 0.5341910419936866 1103 46-47 0.45405432279052604 1103 48-49 0.4597406990876962 1103 50-51 0.40160001609881135 1103 52-53 0.4773280205766568 1103 54-55 0.4957871242594365 1103 56-57 0.37730616723205657 1103 58-59 0.4041955678755542 1103 60-61 0.6343287239871387 1103 62-63 0.9105928685729339 1103 64-65 0.7145091091736688 1103 66-67 0.8284031304862083 1103 68-69 0.7647687175609477 1103 70-71 0.7841734708506145 1103 72-73 0.7429624976544993 1103 74-75 0.7416084390858622 1103 76-77 0.8379758427756734 1103 78-79 1.0868987669310037 1103 80-81 1.0632459432247998 1103 82-83 1.0764288159453166 1103 84-85 1.4528407801696908 1103 86-87 1.4175130024246556 1103 88-89 1.6643293846600677 1103 90-91 1.5268721744454155 1103 92-93 1.6238931124172957 1103 94-95 1.790365755392319 1103 96-97 1.9460164832873232 1103 98-99 2.117184208067382 1103 100-101 2.736373229957902 1104 1 0.0599655591980266 1104 2 -0.00719699122483064 1104 3 0.13284793885275192 1104 4 0.07243224541056748 1104 5 0.02443325140362873 1104 6 0.10611621662064863 1104 7 0.11033043948851429 1104 8 0.15801922552100933 1104 9 0.27370761148293354 1104 10-11 0.15141585253702772 1104 12-13 0.19443874253278182 1104 14-15 0.386952222460792 1104 16-17 0.30029592287770157 1104 18-19 0.3479892838623826 1104 20-21 0.3122615929950854 1104 22-23 0.15029362954761893 1104 24-25 0.20342567568400227 1104 26-27 0.2917067368497541 1104 28-29 0.10735295550041712 1104 30-31 0.2757841716576408 1104 32-33 0.20906976553785483 1104 34-35 0.33738724976021217 1104 36-37 0.2782335707288155 1104 38-39 0.3588950603102248 1104 40-41 0.3129393863739196 1104 42-43 0.4211996878080484 1104 44-45 0.47477942976766485 1104 46-47 0.40146828919604616 1104 48-49 0.4833351518122484 1104 50-51 0.5004487897406094 1104 52-53 0.38813612498213246 1104 54-55 0.5864957666176167 1104 56-57 0.6867197432138354 1104 58-59 0.7129746325517843 1104 60-61 0.6926233228441987 1104 62-63 0.8680451180333364 1104 64-65 0.8059364861400056 1104 66-67 1.0036134923671156 1104 68-69 0.9338611802008572 1104 70-71 1.009740930806725 1104 72-73 1.0687948173504402 1104 74-75 1.0798434037717186 1104 76-77 1.2181545940073875 1104 78-79 1.222765645819056 1104 80-81 1.351069261064346 1104 82-83 1.3029750990502365 1104 84-85 1.5864432304713958 1104 86-87 1.5921719125954858 1104 88-89 1.5900821067892146 1104 90-91 1.6622929171174796 1104 92-93 1.8177047507103588 1104 94-95 2.0795962860631327 1104 96-97 2.23167996071766 1104 98-99 2.4763398629130187 1104 100-101 3.04205319711809 1105 1 0.10902179300187598 1105 2 0.16886942356579837 1105 3 0.12884199481741732 1105 4 0.054514913382753605 1105 5 0.08363010175696672 1105 6 0.09138492854126667 1105 7 -0.04354869077018719 1105 8 0.021198438570721123 1105 9 0.24505276026324196 1105 10-11 0.1719700652663363 1105 12-13 0.19739458006760202 1105 14-15 0.2717311089602248 1105 16-17 0.2644394717154128 1105 18-19 0.2733484186835611 1105 20-21 0.24162859424336602 1105 22-23 0.3017063311744863 1105 24-25 0.4045591178674286 1105 26-27 0.2758865740359937 1105 28-29 0.37500099248210006 1105 30-31 0.4164217072539316 1105 32-33 0.284621043415747 1105 34-35 0.4380202933677495 1105 36-37 0.49877403835701273 1105 38-39 0.2972649425829488 1105 40-41 0.42049336072665255 1105 42-43 0.3404824626652143 1105 44-45 0.35322515680341127 1105 46-47 0.32282804815375243 1105 48-49 0.15856003960863774 1105 50-51 0.1969736180710271 1105 52-53 0.2528550136346368 1105 54-55 0.444079057444732 1105 56-57 0.43030231891122384 1105 58-59 0.46065030071108026 1105 60-61 0.5355152152210181 1105 62-63 0.6293899528229829 1105 64-65 0.5779520370077762 1105 66-67 0.9147086405066247 1105 68-69 0.7057335128822828 1105 70-71 0.7818449116955826 1105 72-73 0.8345997612294234 1105 74-75 0.9664184449749769 1105 76-77 0.9019294793687571 1105 78-79 1.036973050103171 1105 80-81 1.269477507868686 1105 82-83 1.1563859987552156 1105 84-85 1.290387529810861 1105 86-87 1.4377724549662823 1105 88-89 1.4335870819390912 1105 90-91 1.4482403614898693 1105 92-93 1.533618822361742 1105 94-95 1.7627069850704054 1105 96-97 1.9290906912521635 1105 98-99 2.0220998356406845 1105 100-101 2.7153771598595746 1106 1 -0.1506441130305376 1106 2 -0.09929949574846475 1106 3 -0.06364966746097167 1106 4 -0.012944568215750962 1106 5 -0.11986296476927549 1106 6 -0.1670200799427306 1106 7 0.004087492016076055 1106 8 -0.06210710235725969 1106 9 -0.025251785989176767 1106 10-11 -0.28095983936183444 1106 12-13 -0.4218633627092956 1106 14-15 -0.46667653248849206 1106 16-17 -0.34079135152488504 1106 18-19 -0.20222571390083743 1106 20-21 -0.031074144039948237 1106 22-23 -0.037200110812534604 1106 24-25 -0.11740273339932372 1106 26-27 -0.12221403447141199 1106 28-29 -0.04340855055224324 1106 30-31 0.04912678595032105 1106 32-33 0.03918631168559017 1106 34-35 -0.19294629723550116 1106 36-37 -0.5027845304803833 1106 38-39 -0.7171717492431569 1106 40-41 -0.40456807511677084 1106 42-43 -0.22136953546245763 1106 44-45 -0.20776235124175457 1106 46-47 -0.17186213966439823 1106 48-49 -0.18312052326070472 1106 50-51 -0.23047650187514535 1106 52-53 -0.06380653337841125 1106 54-55 -0.0944507243117485 1106 56-57 -0.17647390834289212 1106 58-59 -0.5446090463136741 1106 60-61 -0.335083345424934 1106 62-63 -0.3936243529519814 1106 64-65 -0.06757338572933946 1106 66-67 0.07089610759457088 1106 68-69 -0.0757362665337844 1106 70-71 -0.06645975157747941 1106 72-73 0.029282635256688394 1106 74-75 0.12048198719018188 1106 76-77 0.4497148634890564 1106 78-79 0.3425819963321821 1106 80-81 0.35767743765629945 1106 82-83 0.29635440317859363 1106 84-85 0.3491304354337039 1106 86-87 0.4718459439370868 1106 88-89 0.733083161747178 1106 90-91 0.9425151259857998 1106 92-93 1.1517014389435722 1106 94-95 1.2440480417669129 1106 96-97 1.4623333335785311 1106 98-99 1.5768962197024727 1106 100-101 1.9784929810520104 1107 1 0.038774953765845055 1107 2 0.12300794710469276 1107 3 0.13716369935728423 1107 4 0.11593014237347887 1107 5 0.07645595647328918 1107 6 0.10652720996579035 1107 7 0.1139821351829653 1107 8 0.0868879729461085 1107 9 0.22066667370448556 1107 10-11 0.15230920141586068 1107 12-13 0.12957332137857946 1107 14-15 0.31431493036511426 1107 16-17 0.2214611881253319 1107 18-19 0.29995344550699343 1107 20-21 0.28304042868794 1107 22-23 0.21830906360318636 1107 24-25 0.332516570959001 1107 26-27 0.29692063675041425 1107 28-29 0.38737570550181744 1107 30-31 0.37372877543725735 1107 32-33 0.3734553750882341 1107 34-35 0.5217103314966707 1107 36-37 0.48883594358765947 1107 38-39 0.5766384425409754 1107 40-41 0.5479676157359563 1107 42-43 0.5457205947818125 1107 44-45 0.6458489672646976 1107 46-47 0.5876884157780324 1107 48-49 0.6415979846995086 1107 50-51 0.4159095400413406 1107 52-53 0.43405918002385135 1107 54-55 0.6560132756279202 1107 56-57 0.6742802565326329 1107 58-59 0.5037076576841599 1107 60-61 0.7460972242712103 1107 62-63 0.9006407887933037 1107 64-65 0.6112059230031335 1107 66-67 0.9373218185835057 1107 68-69 1.0178985819167892 1107 70-71 1.0115699172687265 1107 72-73 1.1927486261916265 1107 74-75 1.1048956617077046 1107 76-77 1.1550200363059382 1107 78-79 1.2905252001754057 1107 80-81 1.447188512567493 1107 82-83 1.607171955611907 1107 84-85 1.6646224903067441 1107 86-87 1.5223169199018507 1107 88-89 1.5497371961521829 1107 90-91 1.7695866283576507 1107 92-93 2.0890375023537544 1107 94-95 2.184792741873249 1107 96-97 2.2322457373282383 1107 98-99 2.550018349162414 1107 100-101 2.943270087623052 1108 1 0.02726972232433411 1108 2 0.08169805366904015 1108 3 0.04509009923120644 1108 4 -0.07207240822440752 1108 5 0.11912912688405441 1108 6 0.08185357977417596 1108 7 0.0610824748929204 1108 8 0.11864306964734794 1108 9 0.23563900461357434 1108 10-11 0.1469928419774078 1108 12-13 0.13894393801659533 1108 14-15 0.2550403054097288 1108 16-17 0.3477102371019356 1108 18-19 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2791.0 11 3849.0 12 5657.0 13 7901.0 14 9097.0 15 12430.0 16 16943.0 17 23790.0 18 19799.0 19 11660.0 20 10777.0 21 11034.0 22 11525.0 23 12980.0 24 14643.0 25 16823.0 26 19220.0 27 22294.0 28 26616.0 29 31826.0 30 39038.0 31 48410.0 32 61263.0 33 79894.0 34 109090.0 35 153015.0 36 217353.0 37 290898.0 38 203352.0 39 17671.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.983511624252294 19.859037406572337 15.30461347067948 20.852837498495884 2 14.628587351701766 21.184663997539428 41.84001535230114 22.34673329845767 3 18.75028343795617 26.840605009408495 31.106535208416446 23.302576344218885 4 11.518490112690811 17.399482254499713 39.25198327809532 31.83004435471416 5 12.093634925527532 39.49022357842464 34.249449088408085 14.166692407639744 6 28.956226744739755 38.178068380727815 18.142337791994116 14.723367082538315 7 26.48251014347317 35.57952102053388 20.78653456399156 17.15143427200139 8 25.923121180754414 35.241292251430494 19.91945102696 18.91613554085509 9 26.884803153691905 16.872212293592323 21.201642263254723 35.04134228946105 10-11 24.134607363990025 28.161787356068928 27.22751207375681 20.476093206184235 12-13 25.654840919585226 25.963123058663264 28.193644665342937 20.18839135640857 14-15 22.460659257512656 26.637585704330487 27.97378181462315 22.92797322353371 16-17 20.0522241738424 30.456012528022384 28.31040152259211 21.1813617755431 18-19 19.954076777743555 28.08307158256822 32.31880422312395 19.644047416564277 20-21 21.462942453358952 26.74104192120496 32.298729916169414 19.497285709266677 22-23 20.723054250084722 27.101694937516914 32.08921014125355 20.08604067114482 24-25 20.315641051068546 27.16524625347824 32.56070386470162 19.95840883075159 26-27 20.26476534938577 27.263645877905034 32.47409764983234 19.997491122876852 28-29 19.945524149438803 27.573982296990597 32.67332650469504 19.807167048875556 30-31 20.090076095712913 27.52746240452732 32.40190989697473 19.98055160278504 32-33 19.897064445066228 27.519858706632355 32.24072680173346 20.34235004656796 34-35 20.471148771602337 27.46448830748809 32.08320046450177 19.9811624564078 36-37 20.38604074765103 27.234024807429485 32.220532229496634 20.159402215422848 38-39 20.247200411344007 27.647189773136947 32.12541526766582 19.980194547853216 40-41 20.30849704790106 27.71985282022878 32.052673206603586 19.91897692526657 42-43 20.147918492471252 27.79562765702795 31.634422506149317 20.42203134435148 44-45 20.32842416622365 28.036268577663755 31.19734050570189 20.437966750410702 46-47 20.52205318062122 28.19296028151781 30.55562396907665 20.729362568784325 48-49 20.620059265010095 28.461589238898156 30.247388559547606 20.67096293654414 50-51 20.67375754934681 28.722146928982696 30.054536241626383 20.54955928004411 52-53 20.74877687275503 28.953984404532363 29.77983545379277 20.517403268919843 54-55 20.527120510865345 28.789472958302053 29.878437638948817 20.804968891883778 56-57 20.752895182476767 29.220807361449175 29.39159308741327 20.634704368660785 58-59 20.892257959050763 28.700278295145164 29.668369875847144 20.739093869956925 60-61 20.752315981379567 28.72785425396295 30.019064966326876 20.50076479833061 62-63 20.788222653153078 28.640443790935283 30.085430017671666 20.48590353823997 64-65 20.653205948980595 28.7924751305033 29.969401490528252 20.584917429987854 66-67 20.638267346187675 28.97758966154509 29.626172612037738 20.757970380229498 68-69 20.68944686861139 28.962467854845897 29.599149558971604 20.748935717571108 70-71 20.622633883882653 28.97772621063231 29.601128348677076 20.798511556807963 72-73 20.68442775010589 28.91906711627556 29.456135224142287 20.94036990947626 74-75 20.505228746151662 29.22732502548045 29.334500530623824 20.93294569774406 76-77 20.648148300166312 29.052929867392123 29.377184615056258 20.92173721738531 78-79 20.676234884187487 29.06885380039001 29.236343028182908 21.018568287239592 80-81 20.536553604960297 29.35947751904947 29.12747244159898 20.976496434391258 82-83 20.62842427075837 29.29616297821599 29.05104691415094 21.0243658368747 84-85 20.640151960336933 29.003715621961234 29.116320175124706 21.239812242577127 86-87 20.673018995593118 29.042255583458115 29.235399388755507 21.04932603219326 88-89 20.80653703315173 28.89728771699298 28.963874204073136 21.332301045782152 90-91 20.447921485528887 28.963045968539188 29.336943163883394 21.252089382048535 92-93 20.66341070082498 29.016913249828626 29.357207206024107 20.962468843322295 94-95 20.41420021209047 28.978485621513723 29.190838742296947 21.416475424098863 96-97 20.493078144746445 28.87193279054702 29.068935525273314 21.566053539433216 98-99 20.364974072781393 29.043902416601302 28.852842472241473 21.738281038375828 100-101 20.647929842945 28.229882929320983 29.753128001696545 21.369059226037475 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2402.0 1 1448.0 2 572.0 3 675.5 4 637.0 5 669.0 6 1193.0 7 2503.5 8 4045.5 9 4700.5 10 4195.5 11 3645.0 12 3741.0 13 3933.0 14 4095.0 15 4233.0 16 4329.0 17 4430.0 18 4621.5 19 4935.0 20 5298.0 21 6017.5 22 6942.0 23 8099.0 24 9449.5 25 11232.5 26 13760.0 27 16681.0 28 19123.5 29 21369.5 30 23882.0 31 26848.5 32 30453.0 33 34320.0 34 38658.5 35 41921.5 36 45094.0 37 48713.5 38 50476.5 39 51053.0 40 51887.0 41 53110.5 42 54102.0 43 55061.0 44 56119.5 45 56445.5 46 55545.5 47 56035.0 48 62574.0 49 60728.0 50 50892.5 51 45851.0 52 41136.5 53 37688.5 54 34773.0 55 31165.0 56 27365.5 57 23406.5 58 20715.0 59 18473.5 60 15575.0 61 12644.5 62 9900.0 63 7090.0 64 4774.0 65 3425.5 66 2474.0 67 1814.0 68 1354.0 69 985.0 70 730.0 71 567.0 72 443.0 73 390.5 74 313.5 75 259.0 76 208.5 77 127.5 78 77.0 79 61.5 80 46.0 81 27.5 82 17.0 83 9.0 84 9.0 85 6.0 86 3.0 87 4.5 88 4.5 89 3.5 90 2.5 91 1.0 92 0.0 93 2.5 94 3.5 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3145589881674956 2 0.2642846093543118 3 0.27077374038756497 4 0.26421906257619815 5 0.26605437236338086 6 0.20470258804898703 7 0.1949361181100504 8 0.20162188947764462 9 0.20286727826180434 10-11 0.203916026711623 12-13 0.20706227206107908 14-15 0.20299837181803165 16-17 0.19411678338362956 18-19 0.18805370640811522 20-21 0.187103278125467 22-23 0.19506721166627777 24-25 0.1932974486572087 26-27 0.19890169818592737 28-29 0.20440762754747552 30-31 0.2056530163316352 32-33 0.20463704127087334 34-35 0.20558746955352158 36-37 0.19264198087607204 38-39 0.18451418038997713 40-41 0.17884438408314476 42-43 0.18992178958435477 44-45 0.1922159268183332 46-47 0.1982462304047907 48-49 0.2061118437784309 50-51 0.20073700797311006 52-53 0.20965136979656904 54-55 0.19893447157498417 56-57 0.17481325722915414 58-59 0.15895093692564635 60-61 0.17550149839934767 62-63 0.1683568995849578 64-65 0.17553427178840453 66-67 0.1764847000710527 68-69 0.17392837572461964 70-71 0.17661579362728003 72-73 0.18503855461488647 74-75 0.1895612823047296 76-77 0.1904461638092641 78-79 0.18638226356621665 80-81 0.18461250055714762 82-83 0.17540317823217716 84-85 0.1703560763174247 86-87 0.1749771241744383 88-89 0.18330156499487424 90-91 0.18022086642353183 92-93 0.17566536534463184 94-95 0.17651747346010954 96-97 0.18395703277601091 98-99 0.1840225795541246 100-101 0.16589889540569522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1525628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.136359796981104 #Duplication Level Percentage of deduplicated Percentage of total 1 73.00160977476372 38.06038192975894 2 14.317081208160692 14.928809942225255 3 5.448152081627425 8.521404514691971 4 2.5028419616465634 5.219562761095489 5 1.3710516086345557 3.574081998400046 6 0.8474519573777215 2.650983609630047 7 0.5343986664432157 1.9503120804097267 8 0.3599300510427239 1.5012354114327382 9 0.2495913004903305 1.1711503660104268 >10 1.23190921330011 11.52037638048557 >50 0.08925468727262337 3.1546068207114706 >100 0.042833651609274714 3.9121599802667264 >500 0.002386545644862832 0.8369927266494709 >1k 0.0011304689896718678 0.9933108268382109 >5k 1.256076655190964E-4 0.4681029661286611 >10k+ 2.512153310381928E-4 1.5365276852653578 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13203 0.8654141114347665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10260 0.6725099434462398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7148 0.4685283699565032 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2262 0.14826681209311837 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2054 0.13463308224547532 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2007 0.13155238367413288 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2003 0.1312901965616782 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.11287155191173734 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1660 0.10880765166868987 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 6.55467781136686E-5 0.0 0.0 0.0 0.0 16-17 6.55467781136686E-5 0.0 0.0 0.0 0.0 18-19 9.832016717050291E-5 0.0 0.0 0.0 0.0 20-21 1.310935562273372E-4 0.0 0.0 9.832016717050291E-5 0.0 22-23 1.310935562273372E-4 0.0 0.0 1.9664033434100582E-4 0.0 24-25 3.27733890568343E-4 0.0 0.0 3.932806686820116E-4 0.0 26-27 3.27733890568343E-4 0.0 0.0 5.57147613966183E-4 0.0 28-29 4.2605405773884586E-4 0.0 0.0 0.0015403492856712123 0.0 30-31 4.588274467956802E-4 0.0 0.0 0.0032117921275697614 6.55467781136686E-5 32-33 4.588274467956802E-4 0.0 0.0 0.005931983419287008 6.55467781136686E-5 34-35 4.588274467956802E-4 0.0 0.0 0.008258894042322244 6.55467781136686E-5 36-37 5.899210030230174E-4 0.0 0.0 0.01150345955894884 6.55467781136686E-5 38-39 7.210145592503546E-4 0.0 0.0 0.01684552197521283 6.55467781136686E-5 40-41 7.210145592503546E-4 0.0 0.0 0.0281851145888775 6.55467781136686E-5 42-43 7.210145592503546E-4 0.0 0.0 0.03785326436064362 6.55467781136686E-5 44-45 7.865613373640232E-4 0.0 0.0 0.04965168442110396 6.55467781136686E-5 46-47 8.521081154776918E-4 0.0 0.0 0.07265860353900164 6.55467781136686E-5 48-49 8.521081154776918E-4 0.0 0.0 0.08871756417685045 6.55467781136686E-5 50-51 8.521081154776918E-4 0.0 0.0 0.10972530656228124 6.55467781136686E-5 52-53 8.521081154776918E-4 0.0 0.0 0.13666503236699903 6.55467781136686E-5 54-55 8.521081154776918E-4 0.0 0.0 0.1713065046000729 6.55467781136686E-5 56-57 9.176548935913604E-4 0.0 0.0 0.25061810611761187 6.55467781136686E-5 58-59 9.176548935913604E-4 0.0 0.0 0.31249426465691504 6.55467781136686E-5 60-61 9.176548935913604E-4 0.0 0.0 0.3659476622086118 6.55467781136686E-5 62-63 9.176548935913604E-4 0.0 0.0 0.42844651513999477 6.55467781136686E-5 64-65 9.176548935913604E-4 0.0 0.0 0.48557053226605695 6.55467781136686E-5 66-67 9.83201671705029E-4 0.0 0.0 0.5863487036158225 6.55467781136686E-5 68-69 9.83201671705029E-4 0.0 0.0 0.7225549085360259 6.55467781136686E-5 70-71 9.83201671705029E-4 0.0 0.0 0.7920017199474577 6.55467781136686E-5 72-73 9.83201671705029E-4 0.0 0.0 0.8801293631212851 6.55467781136686E-5 74-75 9.83201671705029E-4 0.0 0.0 1.0379660048189991 6.55467781136686E-5 76-77 9.83201671705029E-4 0.0 0.0 1.2111078192062548 6.55467781136686E-5 78-79 9.83201671705029E-4 0.0 0.0 1.3806445607972586 6.55467781136686E-5 80-81 0.0010487484498186977 0.0 0.0 1.546346815868613 6.55467781136686E-5 82-83 0.0010487484498186977 0.0 0.0 1.7724832003607696 6.55467781136686E-5 84-85 0.0010487484498186977 0.0 0.0 2.108574305138605 6.55467781136686E-5 86-87 0.0010487484498186977 0.0 0.0 2.3708597377604503 6.55467781136686E-5 88-89 0.0010487484498186977 0.0 0.0 2.727696397811262 6.55467781136686E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10595 0.0 30.070572 1 GTATCAA 14855 0.0 27.593224 1 GTACATG 10665 0.0 25.860384 1 TACATGG 10645 0.0 25.361246 2 ACATGGG 10810 0.0 23.874144 3 GAGTACT 9290 0.0 23.519697 12-13 CATGGGA 8080 0.0 22.76649 4 ATCAACG 17925 0.0 22.590929 3 TATCAAC 18165 0.0 22.424011 2 TCAACGC 18115 0.0 22.356186 4 CAACGCA 18220 0.0 22.200533 5 ACACCGA 150 3.0284937E-5 22.171926 6 AACGCAG 18510 0.0 21.92038 6 GTACTTT 9995 0.0 21.692974 14-15 TAGCCCT 1150 0.0 21.493246 4 AGTACTT 9680 0.0 21.49257 12-13 CATGGGG 2730 0.0 20.54546 4 ATGGGAG 2795 0.0 20.237057 5 ACGCAGA 20590 0.0 19.636116 7 CGCAGAG 20595 0.0 19.60828 8 >>END_MODULE