FastQCFastQC Report
Thu 26 May 2016
SRR937584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525628
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169411.1104279680236597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145140.951345937541786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103720.6798511825949707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.2052269622738964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25380.16635772285249092No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22470.14728361042141333No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17310.11346147291476036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113200.032.1591031
GTATCAA164300.029.2149661
TATCACG1205.3804324E-627.7101612
GAGTACT122400.024.06046712-13
ATCAACG203250.023.6522353
TATCAAC204850.023.4906862
GTACATG101500.023.4112991
TCAACGC205750.023.3648454
TACATGG102200.023.333242
CAACGCA207050.023.2181435
AACGCAG210750.022.8308146
AGTACTT127600.022.44710712-13
GTACTTT132500.022.13607414-15
ACATGGG102700.021.9707763
GTATAGG4600.021.6961691
ACGCAGA231550.020.698567
CGCAGAG231150.020.672738
AGAGTAC202450.019.86108410-11
TACTTTT144750.019.67207314-15
GCAGAGT240200.019.6565429
Produced by FastQC (version 0.11.5)