##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937584_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525628 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29000319868277 33.0 31.0 34.0 31.0 34.0 2 32.49784613287118 34.0 31.0 34.0 31.0 34.0 3 32.576396080827045 34.0 31.0 34.0 31.0 34.0 4 36.19276324241558 37.0 35.0 37.0 35.0 37.0 5 36.060221102391935 37.0 35.0 37.0 35.0 37.0 6 36.1682893864035 37.0 36.0 37.0 35.0 37.0 7 36.132645048465285 37.0 35.0 37.0 35.0 37.0 8 36.1384996866864 37.0 36.0 37.0 35.0 37.0 9 37.80251083488242 39.0 38.0 39.0 35.0 39.0 10-11 37.76984428707391 39.0 37.5 39.0 35.0 39.0 12-13 37.68934465020306 39.0 37.0 39.0 35.0 39.0 14-15 38.88114828778706 40.0 38.0 41.0 35.0 41.0 16-17 38.87181672072091 40.0 38.0 41.0 35.5 41.0 18-19 38.93979626750426 40.0 38.0 41.0 35.5 41.0 20-21 38.907317511215055 40.0 38.0 41.0 35.0 41.0 22-23 38.76571844512555 40.0 38.0 41.0 35.0 41.0 24-25 38.70441090488639 40.0 38.0 41.0 34.5 41.0 26-27 38.615365934552855 40.0 38.0 41.0 34.5 41.0 28-29 38.50108217730666 40.0 38.0 41.0 34.0 41.0 30-31 38.46322203053431 40.0 38.0 41.0 35.0 41.0 32-33 38.29071372575753 40.0 37.0 41.0 34.0 41.0 34-35 38.122397137441105 40.0 37.0 41.0 34.0 41.0 36-37 37.93434146462965 40.0 37.0 41.0 34.0 41.0 38-39 37.7790218192115 40.0 37.0 41.0 33.0 41.0 40-41 37.557479280663436 40.0 37.0 41.0 33.0 41.0 42-43 37.289998610408304 40.0 36.5 41.0 33.0 41.0 44-45 36.87066211422444 40.0 36.0 41.0 31.5 41.0 46-47 36.31730146536377 40.0 36.0 41.0 30.5 41.0 48-49 35.81501355507371 40.0 36.0 41.0 28.5 41.0 50-51 35.514723117299894 39.0 35.0 41.0 27.0 41.0 52-53 35.284885633981546 39.0 35.0 41.0 26.5 41.0 54-55 35.08447734310068 39.0 35.0 40.0 26.0 41.0 56-57 34.832480460505444 39.0 35.0 40.0 25.0 41.0 58-59 34.60019119995175 38.0 35.0 40.0 24.0 41.0 60-61 34.27962353863458 38.0 34.0 40.0 23.0 41.0 62-63 33.94127696922185 38.0 34.0 40.0 22.0 41.0 64-65 33.566466727144494 37.0 34.0 40.0 20.5 41.0 66-67 33.16828512586292 37.0 33.0 39.5 19.5 41.0 68-69 32.7354545144688 36.0 33.0 39.0 18.0 40.0 70-71 32.33957721017181 36.0 32.0 39.0 15.0 40.0 72-73 31.905772901388804 35.0 32.0 38.0 10.5 40.0 74-75 31.425131159103007 35.0 31.0 37.5 7.5 39.5 76-77 30.174576961094054 34.0 29.5 36.0 7.0 38.5 78-79 30.71119204681613 34.5 31.0 36.0 4.0 39.0 80-81 30.497777308754166 34.5 31.0 36.0 2.0 38.0 82-83 30.14264552040209 34.0 31.0 35.5 2.0 37.0 84-85 29.74434724585548 34.0 30.0 35.0 2.0 37.0 86-87 29.324315953823607 34.0 30.0 35.0 2.0 36.0 88-89 28.9007415307008 34.0 29.0 35.0 2.0 36.0 90-91 28.408489815341618 34.0 29.0 35.0 2.0 35.5 92-93 27.999536912012626 34.0 28.5 35.0 2.0 35.0 94-95 27.57117101940971 33.5 27.0 35.0 2.0 35.0 96-97 27.06189221749994 33.0 26.5 35.0 2.0 35.0 98-99 26.31701666461287 33.0 25.0 35.0 2.0 35.0 100-101 23.992962242433936 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14958597954930752 1101 2 0.1380318604534807 1101 3 0.10605877743905268 1101 4 0.041650074017169914 1101 5 -0.049595861486160686 1101 6 0.04115788847650492 1101 7 0.03379122665019452 1101 8 0.010726126943993108 1101 9 0.17356964392694607 1101 10-11 0.049607733585467884 1101 12-13 0.2112595666492254 1101 14-15 0.2098671561505725 1101 16-17 -0.010946292612324271 1101 18-19 0.09770331541317745 1101 20-21 0.08359412570055724 1101 22-23 0.07795333026387397 1101 24-25 0.026142477698641642 1101 26-27 0.06463458175722536 1101 28-29 0.05295348535848632 1101 30-31 0.1657206735238148 1101 32-33 0.2089888438855283 1101 34-35 0.23115053514272432 1101 36-37 0.258117505607224 1101 38-39 0.34073471511197084 1101 40-41 0.21651128639579298 1101 42-43 0.34503035459526643 1101 44-45 0.545044250214886 1101 46-47 0.4649030480015668 1101 48-49 0.5396558555770881 1101 50-51 0.6852279315047909 1101 52-53 0.6154310835245127 1101 54-55 0.6164554526968757 1101 56-57 0.7312833334626561 1101 58-59 0.7773545557260562 1101 60-61 0.7171751502196742 1101 62-63 0.7106630233026578 1101 64-65 0.7110863273799382 1101 66-67 0.773665174127224 1101 68-69 0.7535361569321566 1101 70-71 0.8428934780218142 1101 72-73 0.6929265473017843 1101 74-75 0.6008171072328672 1101 76-77 0.4443461998959961 1101 78-79 0.5483436387395528 1101 80-81 0.6668705743217345 1101 82-83 0.5899711717932803 1101 84-85 0.7492711086875197 1101 86-87 0.8502953251385286 1101 88-89 0.9693421796071426 1101 90-91 0.802370385466503 1101 92-93 0.8068967084912977 1101 94-95 0.8854433690498276 1101 96-97 1.238728177990179 1101 98-99 1.4496174067765253 1101 100-101 2.1315958359657152 1102 1 0.12867843145091484 1102 2 0.1496505614210193 1102 3 0.09919426772127338 1102 4 0.05142578945931575 1102 5 -0.08242445799655229 1102 6 0.03426642315017148 1102 7 -0.0010851624320622477 1102 8 0.06836166432906055 1102 9 0.2027128251722914 1102 10-11 0.10112268047041795 1102 12-13 0.11789367538703033 1102 14-15 0.1965628959694783 1102 16-17 0.13613323393603594 1102 18-19 0.18693082602938205 1102 20-21 0.1585068187075791 1102 22-23 0.20633386231200745 1102 24-25 0.09730431166931908 1102 26-27 0.14980013226717404 1102 28-29 0.07022188735497537 1102 30-31 0.16956810033308756 1102 32-33 0.14825591255202397 1102 34-35 0.14944350036804366 1102 36-37 0.1604902661033023 1102 38-39 0.07152141037936843 1102 40-41 0.09948823601575896 1102 42-43 0.0631957114138828 1102 44-45 0.16995237188416468 1102 46-47 0.2080162373808392 1102 48-49 0.31562626443253805 1102 50-51 0.18572438712016748 1102 52-53 0.12452484985939805 1102 54-55 0.26395237433858654 1102 56-57 0.3846460178411064 1102 58-59 0.4073927187541386 1102 60-61 0.3654942179224321 1102 62-63 0.3622087051392171 1102 64-65 0.5203219024472787 1102 66-67 0.6380644294854818 1102 68-69 0.6043504803088666 1102 70-71 0.5356551190236871 1102 72-73 0.44621283892978525 1102 74-75 0.5166223754703516 1102 76-77 0.4733022202922186 1102 78-79 0.6069695863195079 1102 80-81 0.5785370450739222 1102 82-83 0.6707676905736939 1102 84-85 0.5952316162199445 1102 86-87 0.5933893399096455 1102 88-89 0.6651302129287231 1102 90-91 0.7477001039693612 1102 92-93 1.185365540999431 1102 94-95 0.9547326219042738 1102 96-97 1.0404509192699685 1102 98-99 1.3615745534725576 1102 100-101 2.192475216842631 1103 1 0.19666177092746295 1103 2 0.2373426581628877 1103 3 0.1777704548947483 1103 4 0.05438394173873462 1103 5 -0.010259738773008564 1103 6 0.05653708795477286 1103 7 0.05081748223076232 1103 8 0.11003748881031328 1103 9 0.2196071045100112 1103 10-11 0.14073247347386797 1103 12-13 0.16433355893099133 1103 14-15 0.2576604140805685 1103 16-17 0.17474158249913785 1103 18-19 0.22378914116823978 1103 20-21 0.1637257040169544 1103 22-23 0.2329881627219592 1103 24-25 0.2544790638667038 1103 26-27 0.38840959259609775 1103 28-29 0.22164909534326682 1103 30-31 0.1527697464306641 1103 32-33 0.18965194263584095 1103 34-35 0.2422668239605983 1103 36-37 0.28736213053172577 1103 38-39 0.2186727385261733 1103 40-41 0.1364822435980031 1103 42-43 0.16995088604235775 1103 44-45 0.22771445000823576 1103 46-47 0.3449520055383246 1103 48-49 0.1843114702218145 1103 50-51 0.3231775539850972 1103 52-53 0.11241002326493543 1103 54-55 0.1466781846662144 1103 56-57 0.42232632701463046 1103 58-59 0.24842331996573108 1103 60-61 0.15757719100088252 1103 62-63 0.2319756020554351 1103 64-65 0.33054091176131095 1103 66-67 0.27984664140344506 1103 68-69 0.26352427296682635 1103 70-71 0.43223125089393477 1103 72-73 0.46618257609408786 1103 74-75 0.5829574508552007 1103 76-77 0.45641506765846174 1103 78-79 0.5202668186212662 1103 80-81 0.5543128081800042 1103 82-83 0.480778742666196 1103 84-85 0.47540663666645955 1103 86-87 0.598936623737476 1103 88-89 0.809678298653278 1103 90-91 0.8976954145981573 1103 92-93 1.0031364334309565 1103 94-95 0.9815687957992729 1103 96-97 1.248433369333867 1103 98-99 1.6036634132084764 1103 100-101 2.4324083911649446 1104 1 0.1748902654017499 1104 2 0.22542018782214512 1104 3 0.0681114239893077 1104 4 0.05457362599881321 1104 5 -0.06573860279703325 1104 6 0.037149145531508054 1104 7 0.0863850265556394 1104 8 0.06805384529570091 1104 9 0.1990321808273734 1104 10-11 0.0671876383115304 1104 12-13 0.12454990372111752 1104 14-15 0.13498619185149607 1104 16-17 0.21212741919436695 1104 18-19 0.14669179910438856 1104 20-21 0.1718281602826579 1104 22-23 0.19968766532445414 1104 24-25 0.16182063023393312 1104 26-27 0.06855640006744324 1104 28-29 0.019501822970930505 1104 30-31 0.1473617085310437 1104 32-33 0.12286184543429357 1104 34-35 0.07042830345127271 1104 36-37 0.017719699908312236 1104 38-39 -0.07714844740193172 1104 40-41 -0.13012756627843203 1104 42-43 -0.0825703581583781 1104 44-45 -0.16564546627467536 1104 46-47 -0.07490857653051819 1104 48-49 -0.08755189543445852 1104 50-51 -0.3100848029116605 1104 52-53 -0.3599805889933805 1104 54-55 -0.26302134324690485 1104 56-57 -0.2255329901045826 1104 58-59 -0.2217488301373578 1104 60-61 -0.12468724340400428 1104 62-63 -0.1647542645749951 1104 64-65 -0.12486292224634354 1104 66-67 -0.08934075064454561 1104 68-69 -0.08215274419581675 1104 70-71 0.08529452077711142 1104 72-73 0.09798797168032536 1104 74-75 -0.011075175419382077 1104 76-77 -0.10216216656465349 1104 78-79 0.0086566581780545 1104 80-81 0.03134457534780921 1104 82-83 0.141752020750598 1104 84-85 0.32866520569654867 1104 86-87 0.2573171623651298 1104 88-89 0.393036530470134 1104 90-91 0.35124968160668146 1104 92-93 0.643102761565526 1104 94-95 0.8105767267542028 1104 96-97 1.1290566566842841 1104 98-99 1.2037023683042207 1104 100-101 1.7203736812727648 1105 1 0.09484347438623786 1105 2 0.12593064691493083 1105 3 0.0425878285528043 1105 4 -0.0302147442999825 1105 5 -0.028521098266594436 1105 6 -0.016452263197173522 1105 7 0.030776640826452706 1105 8 0.0072918476487018324 1105 9 0.10424224698328288 1105 10-11 0.1109142011519495 1105 12-13 0.004573113397661643 1105 14-15 0.13360751655884684 1105 16-17 0.008664358592895383 1105 18-19 0.1024881627081271 1105 20-21 0.17985879167301277 1105 22-23 0.0868141125650439 1105 24-25 0.06990258948435013 1105 26-27 -0.017062123074310875 1105 28-29 -0.027386593721580255 1105 30-31 0.0850026839269944 1105 32-33 0.06319304790316238 1105 34-35 -0.08716040211263731 1105 36-37 -0.086735859520509 1105 38-39 -0.1931132061161307 1105 40-41 -0.16043263062729807 1105 42-43 -0.17666726977194003 1105 44-45 0.01416913185573776 1105 46-47 -0.03562257846316186 1105 48-49 -0.17860245690101806 1105 50-51 -0.23169559419374508 1105 52-53 -0.2817837796510716 1105 54-55 -0.2952582511181987 1105 56-57 -0.22610442105610673 1105 58-59 -0.03665133924309316 1105 60-61 -0.13425258421843012 1105 62-63 -0.2602431480242302 1105 64-65 -0.232529516716518 1105 66-67 -0.21386191489624906 1105 68-69 -0.2547407512907256 1105 70-71 -0.20530479126296086 1105 72-73 -0.09557108675781834 1105 74-75 -0.17495971400691346 1105 76-77 -0.371659063824346 1105 78-79 -0.24829887561887176 1105 80-81 -0.16165714967839762 1105 82-83 -0.12684151257983345 1105 84-85 -0.0805506442953785 1105 86-87 -0.017378523018795278 1105 88-89 0.14129099874939044 1105 90-91 0.23136452529060136 1105 92-93 0.33515146087525594 1105 94-95 0.3643530731333122 1105 96-97 0.5726685808959111 1105 98-99 0.7709155923089952 1105 100-101 1.4237636909872542 1106 1 -0.10124364749077586 1106 2 0.08069631305500735 1106 3 -0.0703488047629719 1106 4 -0.07451583844309795 1106 5 -0.07629185480512035 1106 6 -0.11110149378637146 1106 7 -0.17253504368586903 1106 8 -0.03891522039880613 1106 9 0.09304629589792057 1106 10-11 -0.031815159330172094 1106 12-13 -0.18861921004742044 1106 14-15 -0.016357377244908378 1106 16-17 -0.008578634795441076 1106 18-19 -0.01689996554650719 1106 20-21 -0.04552957062293217 1106 22-23 -0.011601720329458942 1106 24-25 0.0530728252809709 1106 26-27 -0.11857461502913935 1106 28-29 0.02337111501401523 1106 30-31 -0.07413589778267493 1106 32-33 0.013214989275816436 1106 34-35 -0.29775369682915453 1106 36-37 -0.06933269411181442 1106 38-39 5.779178672540297E-4 1106 40-41 0.037574361356789154 1106 42-43 -0.015414271117670353 1106 44-45 0.09298766207631814 1106 46-47 0.07779091401825866 1106 48-49 0.12962555784359608 1106 50-51 0.06585647999735755 1106 52-53 0.052495958222664285 1106 54-55 -0.09123712889012836 1106 56-57 -0.2446587771499651 1106 58-59 -0.00903007659023558 1106 60-61 -0.03783999864707965 1106 62-63 0.12529090498612305 1106 64-65 0.07699377039364919 1106 66-67 0.2194472829271561 1106 68-69 -0.0788199450184166 1106 70-71 0.05830178529780028 1106 72-73 0.32653777170569853 1106 74-75 0.23409622186576584 1106 76-77 0.22777777427757684 1106 78-79 0.4092694351879871 1106 80-81 0.5341637502104319 1106 82-83 0.3847208594288283 1106 84-85 0.4338231489291253 1106 86-87 0.45820219197740286 1106 88-89 0.5544572850989304 1106 90-91 0.465758358302125 1106 92-93 0.7696586576454862 1106 94-95 0.6230023622328424 1106 96-97 0.8418665505875609 1106 98-99 0.9113988876106163 1106 100-101 1.4826879786240177 1107 1 0.14188327091612507 1107 2 0.17589416821009962 1107 3 0.07864891011745101 1107 4 0.12719159083508913 1107 5 -0.11880299759116042 1107 6 0.06189299326838693 1107 7 0.008159843830441105 1107 8 0.005372734833969162 1107 9 0.0895183126102097 1107 10-11 0.12280108387292188 1107 12-13 0.12668449214795174 1107 14-15 0.04391152024145839 1107 16-17 0.1785939633779705 1107 18-19 0.1542251353230455 1107 20-21 0.1250500805004009 1107 22-23 0.20268140824811098 1107 24-25 0.2537308341348634 1107 26-27 -0.0028365138654677935 1107 28-29 -0.004985484305734644 1107 30-31 0.05974099988469561 1107 32-33 0.04060701171976433 1107 34-35 0.12290627285342026 1107 36-37 0.1486152986783722 1107 38-39 0.06930873379823055 1107 40-41 0.11489021314830694 1107 42-43 0.3280195923935736 1107 44-45 0.2615817856236049 1107 46-47 0.11804107050747348 1107 48-49 0.18669811604626574 1107 50-51 0.11919064291205217 1107 52-53 0.21632243996810985 1107 54-55 0.1524422152880902 1107 56-57 0.1679587783000116 1107 58-59 0.08517812570696037 1107 60-61 0.2538911491164413 1107 62-63 0.09630778319603905 1107 64-65 0.10425777334997832 1107 66-67 0.2968104585646145 1107 68-69 0.2178424983249485 1107 70-71 0.2575278235943017 1107 72-73 0.23070396701931983 1107 74-75 0.31641350657055156 1107 76-77 0.1058551440521569 1107 78-79 0.25571486758841644 1107 80-81 0.2226112641059217 1107 82-83 0.17381412699898036 1107 84-85 0.3119900757826741 1107 86-87 0.3565253063766711 1107 88-89 0.5240778270418076 1107 90-91 0.6008138271287216 1107 92-93 0.7122444627542031 1107 94-95 0.8143432296732804 1107 96-97 0.974058643923005 1107 98-99 1.383430683201773 1107 100-101 2.0524093901189993 1108 1 -3.917415251137868E-4 1108 2 0.090335915086591 1108 3 0.012272545453868133 1108 4 0.04661251178147552 1108 5 -0.07100300302284523 1108 6 0.0035467278019467585 1108 7 0.028417359353781535 1108 8 -0.03889628056563055 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2316 46-47 -0.4098618777771108 2316 48-49 -0.3505056468540886 2316 50-51 -0.4113855707514418 2316 52-53 -0.4584204357380486 2316 54-55 -0.4998378160072008 2316 56-57 -0.39905571468755596 2316 58-59 -0.4039880747755831 2316 60-61 -0.2706388792783301 2316 62-63 -0.46626413064405625 2316 64-65 -0.21091363191644774 2316 66-67 -0.3678504813937238 2316 68-69 -0.3057250637347124 2316 70-71 -0.12746627701168478 2316 72-73 -0.19518007192929332 2316 74-75 -0.19989797739818016 2316 76-77 -0.013892970235168889 2316 78-79 -0.013237471663813949 2316 80-81 -0.09535144486353175 2316 82-83 -0.20283355432982475 2316 84-85 -0.36088890416442965 2316 86-87 -0.3057736060061025 2316 88-89 -0.46919600623258617 2316 90-91 -0.6389724528834044 2316 92-93 -0.6280827253560126 2316 94-95 -0.665044706305558 2316 96-97 -0.7793492082486217 2316 98-99 -0.9251661387424619 2316 100-101 -1.4135711073267778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 6.0 5 28.0 6 47.0 7 130.0 8 368.0 9 661.0 10 1283.0 11 2351.0 12 3897.0 13 5902.0 14 7574.0 15 11465.0 16 16811.0 17 26152.0 18 23533.0 19 11421.0 20 8225.0 21 7729.0 22 7549.0 23 7963.0 24 8893.0 25 10434.0 26 12312.0 27 15179.0 28 18667.0 29 23353.0 30 29442.0 31 37195.0 32 48162.0 33 64373.0 34 90606.0 35 135255.0 36 210756.0 37 343213.0 38 305596.0 39 29093.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53531968804653 20.32655047210734 15.90810579348722 20.230024046358913 2 15.078511930824554 21.53506621535525 41.168817037967315 22.21760481585288 3 18.89648066238952 26.640242575516442 31.890473955643184 22.572802806450852 4 11.810873948301944 17.964929851838065 38.935245026965944 31.28895117289405 5 11.999701069463327 39.38521264056619 34.310342341019435 14.304743948951055 6 28.92520326055893 38.51023971767692 17.659940693275164 14.90461632848899 7 26.132517232247963 35.982166032610834 20.637140901976103 17.248175833165096 8 26.210911178871914 35.057563180539425 19.670194831243265 19.061330809345396 9 26.84081571654427 16.526243619021148 21.147553663147242 35.485387001287336 10-11 24.156511285844257 28.234897366854828 26.91465416208932 20.693937185211595 12-13 25.941076200174294 26.036810250262114 28.194874016808686 19.82723953275491 14-15 22.569394373988942 26.456875463743458 28.44953029178804 22.524199870479567 16-17 19.82544237520549 30.130411869734957 28.906850162687103 21.137295592372453 18-19 19.700182482230268 27.27929088873566 33.551101579152984 19.4694250498811 20-21 21.003666362638562 26.005021543608482 33.79952432656354 19.191787767189414 22-23 20.44720120711421 26.194791370339445 33.67485523937122 19.683152183175125 24-25 20.056730940976554 26.313291787450698 34.172903191975735 19.457074079597017 26-27 20.042291446535273 26.466042301934184 34.033176368179426 19.458489883351117 28-29 19.688357044347374 26.701798383840504 34.177847025941404 19.43199754587072 30-31 19.94582539258565 26.63906044909537 33.8885274699344 19.52658668838458 32-33 19.711817035345444 26.597866583465958 33.798114612474336 19.89220176871426 34-35 20.16114019931464 26.502922075368307 33.719032424680194 19.61690530063685 36-37 20.160222544421053 26.298481674431773 33.776713589420226 19.764582191726948 38-39 20.024049112889905 26.769009221120744 33.716476100333765 19.490465565655583 40-41 20.067146119499643 26.853925072167 33.57420681843804 19.50472198989531 42-43 19.984464008285862 26.87189034343064 33.16596634523989 19.97767930304361 44-45 20.143508116002064 27.09785740691702 32.6199440492702 20.13869042781071 46-47 20.335855136376626 27.429851838062753 31.93966681261749 20.29462621294313 48-49 20.55357549598444 27.733113096086694 31.422709671921883 20.290601736006987 50-51 20.581032859910803 28.060936217741155 31.115678264950564 20.24235265739748 52-53 20.765481493522667 28.354749650635675 30.580029994205667 20.299738861635998 54-55 20.41441294994586 28.20304163269159 30.729771608806338 20.652773808556212 56-57 20.706423846442252 28.524843539840646 30.237646398728916 20.53108621498819 58-59 20.859541120115782 28.069883352953667 30.574655158400343 20.495920368530204 60-61 20.751093975726718 28.045368857939156 30.926837997205087 20.27669916912904 62-63 20.751225069282945 27.978445597485102 30.97740733651978 20.292921996712174 64-65 20.65401919734037 28.03638894933758 30.901078113406417 20.40851373991563 66-67 20.634322390517216 28.289202872522008 30.58278295888644 20.493691778074343 68-69 20.773773160954047 28.174496010823084 30.518514342945984 20.533216485276885 70-71 20.74984858694256 28.26812958335846 30.400169635061758 20.581852194637225 72-73 20.7866667278437 28.232490641552623 30.25713362440492 20.723709006198764 74-75 20.625965176307723 28.593864297194337 30.01845797271681 20.76171255378113 76-77 20.56553104688692 28.324270398812818 30.333115281051477 20.777083273248785 78-79 20.675420220394486 28.360288353386277 30.065913840071108 20.898377586148133 80-81 20.59617416565506 28.661869079487268 29.954451543888812 20.78750521096886 82-83 20.648160589922163 28.605096272019665 29.82879147890209 20.917951659156085 84-85 20.49774977615746 28.41394286673143 29.91975097435413 21.16855638275698 86-87 20.4702588048987 28.44064870335363 29.94727417168537 21.141818320062296 88-89 20.557534339957055 28.22457374930193 29.821358810929006 21.39653309981201 90-91 20.29975998355971 28.242163904057666 30.106314533301916 21.351761579080712 92-93 20.331683252686517 28.209299795094594 30.231609969178976 21.227406983039916 94-95 20.18155802069705 28.176560734333666 30.18737201991573 21.454509225053553 96-97 20.135937463129938 28.070669914291035 30.05342717884045 21.73996544373858 98-99 20.035670591720844 28.190025540327202 29.891490479484983 21.882813388466968 100-101 19.919794290546573 27.92161670061834 30.423890616063105 21.734698392771982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1019.0 1 721.5 2 614.0 3 842.5 4 706.5 5 647.0 6 1306.0 7 3175.5 8 5202.0 9 5936.0 10 5057.0 11 4007.5 12 4044.0 13 4368.5 14 4707.5 15 4906.5 16 4927.5 17 5076.0 18 5268.0 19 5514.0 20 5918.0 21 6538.0 22 7386.5 23 8517.0 24 9882.5 25 11596.5 26 14093.5 27 16918.0 28 18968.5 29 21096.5 30 23804.0 31 26702.5 32 30079.0 33 33736.0 34 37687.5 35 41030.5 36 44685.5 37 48196.5 38 50069.0 39 50792.0 40 51528.5 41 52474.0 42 53372.5 43 54925.5 44 56351.5 45 56716.0 46 55457.5 47 55779.5 48 63451.0 49 61345.0 50 50634.5 51 45969.5 52 41341.0 53 37678.5 54 34452.0 55 30525.5 56 26662.5 57 22988.5 58 20489.5 59 18321.0 60 15342.5 61 12129.5 62 9137.0 63 6588.5 64 4453.5 65 3191.5 66 2339.5 67 1708.0 68 1238.5 69 826.0 70 584.0 71 469.0 72 404.5 73 379.5 74 326.0 75 275.5 76 212.0 77 131.0 78 75.0 79 51.5 80 33.0 81 17.0 82 8.0 83 4.5 84 3.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04306423322068027 2 0.0 3 0.0 4 0.0 5 0.012453887841597035 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003113471960399259 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.83201671705029E-5 22-23 3.932806686820116E-4 24-25 3.6050727962517727E-4 26-27 0.0015731226747280464 28-29 0.0023596840120920694 30-31 3.6050727962517727E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009045455379686268 44-45 0.0 46-47 0.0 48-49 3.27733890568343E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.55467781136686E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.966403343410058E-4 84-85 1.310935562273372E-4 86-87 0.0 88-89 0.0 90-91 0.007046278647219374 92-93 0.0030151517932287555 94-95 0.0 96-97 0.0 98-99 9.83201671705029E-5 100-101 0.01150345955894884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1525628.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.769031764442474 #Duplication Level Percentage of deduplicated Percentage of total 1 71.53198272097468 35.60077520213739 2 15.01063143741547 14.941291856261389 3 5.608186230848577 8.373419959920351 4 2.6996575415021873 5.374373677445561 5 1.4885654753562219 3.704223121322811 6 0.876443298554786 2.617184061930353 7 0.5850703547366815 2.038286954852647 8 0.4104760400694945 1.6343196061408987 9 0.29390376046166594 1.316457503109517 >10 1.3454538060667698 11.940320429486723 >50 0.09813509706865466 3.324053330197623 >100 0.04662148620395718 4.1721861332364645 >500 0.0031607031832791045 1.1175219116701651 >1k 0.0013169596596996267 1.1040854312072688 >5k 0.0 0.0 >10k+ 3.9508789790988806E-4 2.7415008210808214 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16941 1.1104279680236597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14514 0.951345937541786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10372 0.6798511825949707 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.2052269622738964 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2538 0.16635772285249092 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2247 0.14728361042141333 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1731 0.11346147291476036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.832016717050291E-5 0.0 22-23 0.0 0.0 0.0 1.9664033434100582E-4 0.0 24-25 0.0 0.0 0.0 3.932806686820116E-4 0.0 26-27 0.0 0.0 0.0 5.899210030230174E-4 0.0 28-29 1.966403343410058E-4 0.0 0.0 0.001638669452841715 0.0 30-31 1.966403343410058E-4 0.0 0.0 0.0033428856837970987 0.0 32-33 1.966403343410058E-4 0.0 0.0 0.006030303586457512 0.0 34-35 2.294137233978401E-4 0.0 0.0 0.008324440820435913 0.0 36-37 2.621871124546744E-4 0.0 0.0 0.011765646671403514 0.0 38-39 3.932806686820116E-4 0.0 0.0 0.017337122811065343 0.0 40-41 3.932806686820116E-4 0.0 0.0 0.02870948881378685 0.0 42-43 3.932806686820116E-4 0.0 0.0 0.03844318536366663 0.0 44-45 3.932806686820116E-4 0.0 0.0 0.050110511867899644 0.0 46-47 5.243742249093488E-4 0.0 0.0 0.07360903182164985 0.0 48-49 5.243742249093488E-4 0.0 0.0 0.08986463279383965 0.0 50-51 5.243742249093488E-4 0.0 0.0 0.11106901551361145 0.0 52-53 5.57147613966183E-4 0.0 0.0 0.13840202198701124 0.0 54-55 5.899210030230174E-4 0.0 0.0 0.17386282894650595 0.0 56-57 5.899210030230174E-4 0.0 0.0 0.25451813941537516 0.0 58-59 5.899210030230174E-4 0.0 0.0 0.31754136657166754 0.0 60-61 5.899210030230174E-4 0.0 0.0 0.3723712464637513 0.0 62-63 5.899210030230174E-4 0.0 0.0 0.43582052767778257 0.0 64-65 5.899210030230174E-4 0.0 0.0 0.4936983327521519 0.0 66-67 5.899210030230174E-4 0.0 0.0 0.5956563461079634 0.0 68-69 5.899210030230174E-4 0.0 0.0 0.7326163389764739 0.0 70-71 7.53787948307189E-4 0.0 0.0 0.802620298001872 0.0 72-73 8.521081154776917E-4 0.0 0.0 0.8910756750662678 0.0 74-75 9.176548935913604E-4 0.0 0.0 1.0509114934964487 0.0 76-77 9.176548935913604E-4 0.0 0.0 1.227658380679956 0.0 78-79 9.176548935913604E-4 0.0 0.0 1.3999153135626772 0.0 80-81 9.83201671705029E-4 0.0 0.0 1.5679772526461235 0.0 82-83 0.0011142952279323663 0.0 0.0 1.7953262525333828 0.0 84-85 0.0011798420060460347 0.0 0.0 2.1358089914448346 0.0 86-87 0.0012453887841597034 0.0 0.0 2.4028793388689773 0.0 88-89 0.0012453887841597034 0.0 0.0 2.764107633053405 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11320 0.0 32.159103 1 GTATCAA 16430 0.0 29.214966 1 TATCACG 120 5.3804324E-6 27.710161 2 GAGTACT 12240 0.0 24.060467 12-13 ATCAACG 20325 0.0 23.652235 3 TATCAAC 20485 0.0 23.490686 2 GTACATG 10150 0.0 23.411299 1 TCAACGC 20575 0.0 23.364845 4 TACATGG 10220 0.0 23.33324 2 CAACGCA 20705 0.0 23.218143 5 AACGCAG 21075 0.0 22.830814 6 AGTACTT 12760 0.0 22.447107 12-13 GTACTTT 13250 0.0 22.136074 14-15 ACATGGG 10270 0.0 21.970776 3 GTATAGG 460 0.0 21.696169 1 ACGCAGA 23155 0.0 20.69856 7 CGCAGAG 23115 0.0 20.67273 8 AGAGTAC 20245 0.0 19.861084 10-11 TACTTTT 14475 0.0 19.672073 14-15 GCAGAGT 24020 0.0 19.656542 9 >>END_MODULE