FastQCFastQC Report
Thu 26 May 2016
SRR937583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050785
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79290.38663243587211726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61810.3013967822077887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.2027028674385662No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28110.13706946364440933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.044.026161
GTATCAA83850.040.4316141
ATCAACG104850.032.0506553
TATCAAC105400.031.8841862
TCAACGC106050.031.735914
CAACGCA106750.031.5278035
AACGCAG109600.030.7698866
ACGCAGA124500.026.8979427
CGCAGAG124300.026.826588
GCAGAGT135250.024.021919
GTACATG80650.023.7593881
GAGTACT73950.023.124612-13
TACATGG80150.023.010432
ACATGGG80850.021.693633
GTGTAGC13650.021.248671
GTACTTT79750.021.23382214-15
AGAGTAC121050.020.4639210-11
CAGAGTA131200.020.40137310-11
ATACCGT2601.4924808E-820.0954366
AGTACTT78550.020.01666312-13
Produced by FastQC (version 0.11.5)