##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937583_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050785 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.459420660868886 31.0 31.0 34.0 30.0 34.0 2 31.599456793374245 33.0 31.0 34.0 30.0 34.0 3 31.6186904039185 33.0 31.0 34.0 30.0 34.0 4 35.21940330166253 37.0 35.0 37.0 33.0 37.0 5 35.202568284827514 37.0 35.0 37.0 33.0 37.0 6 35.25842835792148 37.0 35.0 37.0 33.0 37.0 7 35.186727521412536 37.0 35.0 37.0 32.0 37.0 8 35.16866468206077 37.0 35.0 37.0 32.0 37.0 9 36.761808283169614 39.0 37.0 39.0 33.0 39.0 10-11 36.62243141041114 39.0 37.0 39.0 32.0 39.0 12-13 36.48744310105643 39.0 37.0 39.0 32.0 39.0 14-15 37.586344497351014 40.0 37.0 41.0 32.0 41.0 16-17 37.48218096972623 40.0 37.0 41.0 32.0 41.0 18-19 37.40242858222583 40.0 37.0 41.0 32.0 41.0 20-21 37.287801988019226 40.0 37.0 41.0 32.0 41.0 22-23 37.199620389265576 39.5 37.0 41.0 31.5 41.0 24-25 37.23496904843755 40.0 37.0 41.0 31.0 41.0 26-27 37.14196929468472 40.0 37.0 41.0 31.0 41.0 28-29 37.008469439751124 39.5 36.5 41.0 31.0 41.0 30-31 36.765695331299966 39.0 36.0 41.0 30.0 41.0 32-33 36.60947588362505 39.0 36.0 41.0 30.0 41.0 34-35 36.591251642663664 39.0 36.0 41.0 30.0 41.0 36-37 36.43073042761674 39.0 35.5 41.0 30.0 41.0 38-39 36.26220325387595 39.0 35.0 41.0 30.0 41.0 40-41 36.017066391650026 39.0 35.0 40.5 29.0 41.0 42-43 35.73791962589935 39.0 35.0 40.0 27.5 41.0 44-45 35.493148964908556 39.0 35.0 40.0 27.0 41.0 46-47 35.14051399829821 38.0 35.0 40.0 25.5 41.0 48-49 34.80706022328035 38.0 34.0 40.0 24.0 41.0 50-51 33.92419073671789 37.5 33.5 39.5 23.5 40.5 52-53 34.02608050088136 38.0 33.5 39.5 23.0 40.5 54-55 34.28796265820162 38.0 34.0 40.0 23.0 41.0 56-57 34.02983028450082 38.0 33.5 40.0 22.0 41.0 58-59 33.74667480988987 38.0 33.0 40.0 20.5 41.0 60-61 33.39036612809241 37.5 33.0 40.0 19.5 41.0 62-63 32.98102019470593 37.0 32.0 40.0 17.5 41.0 64-65 32.563049515185654 36.0 31.5 39.0 14.0 41.0 66-67 32.09548197397582 36.0 31.0 39.0 10.0 40.5 68-69 31.552429679366682 35.0 31.0 39.0 7.5 40.0 70-71 31.069517038597418 35.0 30.0 38.0 6.5 40.0 72-73 30.57767708462857 35.0 29.5 37.5 2.0 39.5 74-75 30.0139609954237 34.0 29.0 37.0 2.0 39.0 76-77 29.490933715625967 34.0 29.0 36.0 2.0 39.0 78-79 28.892209568531076 34.0 28.0 36.0 2.0 38.0 80-81 28.30138898031729 34.0 26.5 35.0 2.0 37.0 82-83 27.751903051758227 33.0 26.0 35.0 2.0 37.0 84-85 27.186912572502727 33.0 25.5 35.0 2.0 36.0 86-87 26.594338997018212 32.5 25.0 35.0 2.0 36.0 88-89 26.054209485635987 32.0 23.5 35.0 2.0 35.0 90-91 25.428748503621783 32.0 20.5 34.0 2.0 35.0 92-93 24.73020965142616 31.0 18.0 34.0 2.0 35.0 94-95 23.918618724049573 31.0 7.5 34.0 2.0 35.0 96-97 23.00674692861514 31.0 2.0 34.0 2.0 35.0 98-99 21.874084801673504 30.0 2.0 34.0 2.0 35.0 100-101 19.051862335642205 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.174196627971547 1101 2 0.3237405526724686 1101 3 0.30581149215874603 1101 4 0.2467194665478516 1101 5 0.17154523596298077 1101 6 0.2607699479465211 1101 7 0.2674423304493416 1101 8 0.32256114171509154 1101 9 0.20141340595525747 1101 10-11 0.3063525335879689 1101 12-13 0.34004549149960894 1101 14-15 0.4417331327665295 1101 16-17 0.42039119697336247 1101 18-19 0.5208951146159535 1101 20-21 0.5516886803822771 1101 22-23 0.460394174300923 1101 24-25 0.5913972694582625 1101 26-27 0.5570004418683112 1101 28-29 0.37178623947949063 1101 30-31 0.40359107058199584 1101 32-33 0.4058289156024273 1101 34-35 0.44718967358277695 1101 36-37 0.5623922027232098 1101 38-39 0.5566476607473803 1101 40-41 0.6705008728870396 1101 42-43 0.6504837640469745 1101 44-45 0.7484146628003785 1101 46-47 0.4822966815355372 1101 48-49 0.6415257105971293 1101 50-51 0.6363905067884588 1101 52-53 0.5540298497879377 1101 54-55 0.5955832825339868 1101 56-57 0.5700786114217422 1101 58-59 0.6499965716739524 1101 60-61 0.7286174084492458 1101 62-63 0.7246479304242257 1101 64-65 0.782025886087041 1101 66-67 0.8264368384825431 1101 68-69 0.7972437935808898 1101 70-71 0.9033489441125901 1101 72-73 0.9339779354449185 1101 74-75 0.8990511999991497 1101 76-77 0.8350198753266369 1101 78-79 0.8948823163966253 1101 80-81 1.0819756541951158 1101 82-83 0.9641895943555063 1101 84-85 0.9743155006901709 1101 86-87 1.0532768475964147 1101 88-89 1.2948601411033813 1101 90-91 1.399737616303515 1101 92-93 1.4313039054701377 1101 94-95 1.5586171023312403 1101 96-97 1.7446947187803907 1101 98-99 1.900592689597783 1101 100-101 1.9006285843189197 1102 1 0.07602361309903571 1102 2 0.26024911449619026 1102 3 0.3059146692631849 1102 4 0.1185856964939731 1102 5 0.23006541365271005 1102 6 0.2572619263956355 1102 7 0.19355345406676605 1102 8 0.23097926786879697 1102 9 0.3219570930172253 1102 10-11 0.1542024165288538 1102 12-13 0.4185238730823073 1102 14-15 0.37887435783518697 1102 16-17 0.40974472675589624 1102 18-19 0.34474741002513554 1102 20-21 0.5368771380534056 1102 22-23 0.5055229018083196 1102 24-25 0.6474111814838324 1102 26-27 0.33494502263159376 1102 28-29 0.4349859678423087 1102 30-31 0.376264145484825 1102 32-33 0.42180589022461135 1102 34-35 0.40172702744647637 1102 36-37 0.5846095162165241 1102 38-39 0.6257275460894718 1102 40-41 0.6041556497993739 1102 42-43 0.5124665653616418 1102 44-45 0.4951401966949973 1102 46-47 0.526542775813148 1102 48-49 0.5593146627679815 1102 50-51 0.6379015823816374 1102 52-53 0.6533087356252807 1102 54-55 0.422612494786172 1102 56-57 0.3681497623529566 1102 58-59 0.393361814871767 1102 60-61 0.5318258412077839 1102 62-63 0.47673492185489863 1102 64-65 0.5609708157765283 1102 66-67 0.6725505321478451 1102 68-69 0.7071668984988833 1102 70-71 0.6439495098983095 1102 72-73 0.683871944419451 1102 74-75 0.8014329965564109 1102 76-77 0.6330122364690141 1102 78-79 0.7222937529220701 1102 80-81 0.7986672709615839 1102 82-83 0.837754494630147 1102 84-85 0.8899593070623908 1102 86-87 1.0507213383142293 1102 88-89 1.1468858828529633 1102 90-91 0.9479574360624952 1102 92-93 0.9404913576334799 1102 94-95 0.9587200918409415 1102 96-97 1.3013525497350251 1102 98-99 1.2886928989535065 1102 100-101 1.3743104499110466 1103 1 0.13707310145754548 1103 2 0.24434557663685652 1103 3 0.17315189425365674 1103 4 0.22172539973121985 1103 5 0.04549203681887093 1103 6 0.04168419713644056 1103 7 0.09482806111969211 1103 8 0.10250480720268484 1103 9 0.2691053550745508 1103 10-11 0.18628073970534587 1103 12-13 0.1290337159034749 1103 14-15 0.21600044371545124 1103 16-17 0.30487814444270356 1103 18-19 0.18001505569069565 1103 20-21 0.20477875792496292 1103 22-23 0.15345216064940104 1103 24-25 0.24078619884722485 1103 26-27 0.156872346566729 1103 28-29 0.13428729137853423 1103 30-31 0.1808934057505951 1103 32-33 0.09455957838865459 1103 34-35 0.21657865497574846 1103 36-37 0.2581478964518098 1103 38-39 0.25160462034783393 1103 40-41 0.3677337165318164 1103 42-43 0.1471660271853139 1103 44-45 0.11192307941102086 1103 46-47 0.28089831767585594 1103 48-49 0.38675375285463787 1103 50-51 0.2224148106204069 1103 52-53 0.17971712989375987 1103 54-55 0.2793967309568899 1103 56-57 0.32624297620235865 1103 58-59 0.2806650074220727 1103 60-61 0.4422825698365571 1103 62-63 0.4077200854982621 1103 64-65 0.6241074737407217 1103 66-67 0.42440563324735336 1103 68-69 0.27658721474425363 1103 70-71 0.530158919613978 1103 72-73 0.5207884451691918 1103 74-75 0.43006472954829533 1103 76-77 0.5774831959615412 1103 78-79 0.548437711527729 1103 80-81 0.7854330217905741 1103 82-83 0.8048045510834534 1103 84-85 0.8703265340474289 1103 86-87 0.8487190044953117 1103 88-89 0.978395131756848 1103 90-91 0.7952998787988967 1103 92-93 0.7744792317479465 1103 94-95 1.0577856293227335 1103 96-97 1.2297566886350886 1103 98-99 1.5534741601951936 1103 100-101 1.1932871090506048 1104 1 0.2809937327633918 1104 2 0.30267284500649083 1104 3 0.17426645099997984 1104 4 0.15635241178434 1104 5 0.17244856482593462 1104 6 0.17885531786289022 1104 7 0.16915602146958264 1104 8 0.1667785556746182 1104 9 0.14131178323778926 1104 10-11 0.12540415929518645 1104 12-13 0.3501105966673066 1104 14-15 0.34477263151511295 1104 16-17 0.33996111562392883 1104 18-19 0.30016823741458154 1104 20-21 0.391133186519788 1104 22-23 0.5117315850714022 1104 24-25 0.3546044499617338 1104 26-27 0.36295023984724395 1104 28-29 0.4246961116039998 1104 30-31 0.4044538243896483 1104 32-33 0.4774428204603325 1104 34-35 0.4053995021424939 1104 36-37 0.3589791287285493 1104 38-39 0.4568696846026583 1104 40-41 0.5522363320685315 1104 42-43 0.5058867241752196 1104 44-45 0.5066983806713381 1104 46-47 0.7543577662135448 1104 48-49 0.6793420180677856 1104 50-51 0.6576824450232692 1104 52-53 0.662600867524219 1104 54-55 0.7079971851139888 1104 56-57 0.706766006958766 1104 58-59 0.7169870557557232 1104 60-61 0.7939542810663482 1104 62-63 0.8936391206543277 1104 64-65 0.9540377824085198 1104 66-67 0.9186877859456786 1104 68-69 0.8414752308490137 1104 70-71 0.7742871675231129 1104 72-73 0.7295145369921805 1104 74-75 0.8072241550875496 1104 76-77 0.48530881243102897 1104 78-79 0.6722181582649576 1104 80-81 0.8636042657309595 1104 82-83 0.7430736949133099 1104 84-85 0.8527821764684305 1104 86-87 0.841995060243871 1104 88-89 0.7995921179485492 1104 90-91 0.8038495066455162 1104 92-93 0.8457446388393457 1104 94-95 0.9171180035245747 1104 96-97 1.2296210857326493 1104 98-99 1.3209669909238038 1104 100-101 1.4996633962926929 1105 1 0.34373695627585477 1105 2 0.33005870759237155 1105 3 0.3830226561034955 1105 4 0.3626791582226545 1105 5 0.3821140887245704 1105 6 0.38916520699943646 1105 7 0.3487634822353769 1105 8 0.3272533778338911 1105 9 0.4265917432749049 1105 10-11 0.5440140075894462 1105 12-13 0.3954791903554735 1105 14-15 0.49876991715770913 1105 16-17 0.49634819622556847 1105 18-19 0.5706768666504942 1105 20-21 0.5755332695642537 1105 22-23 0.6364419833539401 1105 24-25 0.5933944666748872 1105 26-27 0.580070840632267 1105 28-29 0.6290434124547986 1105 30-31 0.6119836276984643 1105 32-33 0.7174233149828879 1105 34-35 0.7960396380960759 1105 36-37 0.7812726020770242 1105 38-39 0.8851986141313617 1105 40-41 0.7313608022180844 1105 42-43 0.9461153321785858 1105 44-45 0.7869267710909398 1105 46-47 0.7995713314730395 1105 48-49 0.6942531917644459 1105 50-51 0.7035445685410338 1105 52-53 0.7321217118217334 1105 54-55 0.8413907619507413 1105 56-57 0.8128742403735529 1105 58-59 0.6527520235918587 1105 60-61 0.839884626158721 1105 62-63 0.9175830801438565 1105 64-65 0.8143758667739007 1105 66-67 0.9884952288514697 1105 68-69 0.9074245283934417 1105 70-71 0.882233643731464 1105 72-73 0.9965905557714727 1105 74-75 0.9396668229001541 1105 76-77 0.9076812732172215 1105 78-79 0.9180178851066785 1105 80-81 0.9103808076632056 1105 82-83 0.8762023181928669 1105 84-85 0.9945731265252533 1105 86-87 0.9547529706601061 1105 88-89 0.9180820005303829 1105 90-91 0.9840056864549176 1105 92-93 0.942873235827534 1105 94-95 1.1802706018146374 1105 96-97 1.4703604036166276 1105 98-99 1.3818515731652496 1105 100-101 1.1437769396578155 1106 1 0.16880894180541972 1106 2 0.25720592961090105 1106 3 0.16477817417932172 1106 4 0.22882909344789937 1106 5 0.20448704465329115 1106 6 0.12462070796494373 1106 7 0.196634714911184 1106 8 0.27051753661758937 1106 9 0.2906610644843539 1106 10-11 0.0774810457943218 1106 12-13 0.11678201844571845 1106 14-15 0.15853721690594114 1106 16-17 0.14840363363848752 1106 18-19 0.23526023053795342 1106 20-21 0.23275577695835636 1106 22-23 0.187351029820654 1106 24-25 0.1367749755844656 1106 26-27 0.108120509425504 1106 28-29 0.19364559768615663 1106 30-31 0.21520476626754714 1106 32-33 0.30855039571911647 1106 34-35 0.3969010201204739 1106 36-37 0.3077146347821724 1106 38-39 0.3113329056422032 1106 40-41 0.2844291701386865 1106 42-43 0.34775767775801825 1106 44-45 0.27158370932271936 1106 46-47 0.20966886025502873 1106 48-49 0.38081866315355484 1106 50-51 0.372140545173238 1106 52-53 0.28857767721001437 1106 54-55 0.374257472615362 1106 56-57 0.3506743430354291 1106 58-59 0.30180142350874917 1106 60-61 0.23491481363885924 1106 62-63 0.4126511547448217 1106 64-65 0.5587416852579281 1106 66-67 0.671587306776722 1106 68-69 0.3876115196671819 1106 70-71 0.33148421203827283 1106 72-73 0.638519621303967 1106 74-75 0.3714602280968009 1106 76-77 0.4906447793647146 1106 78-79 0.5587553395810261 1106 80-81 0.4525775273718402 1106 82-83 0.4346110591182928 1106 84-85 0.4752482505750173 1106 86-87 0.7428784136949851 1106 88-89 0.9007245660995409 1106 90-91 0.7715985099009366 1106 92-93 0.9059699579804992 1106 94-95 1.0693134020395156 1106 96-97 1.1956597118711336 1106 98-99 1.026360384976254 1106 100-101 0.9837231643895485 1107 1 0.2576408506373262 1107 2 0.3560093484143181 1107 3 0.4016052846906426 1107 4 0.26800438209686916 1107 5 0.3041046784814512 1107 6 0.3575821714527265 1107 7 0.37262961669556205 1107 8 0.3948960041539564 1107 9 0.4405695964981504 1107 10-11 0.5385051873447821 1107 12-13 0.5051640847225158 1107 14-15 0.528145104145409 1107 16-17 0.6198446292900073 1107 18-19 0.7735973125592466 1107 20-21 0.6342560018796348 1107 22-23 0.6984531440806663 1107 24-25 0.6732744507681545 1107 26-27 0.6142631095154698 1107 28-29 0.5054224757788219 1107 30-31 0.6467319817421355 1107 32-33 0.610915817821386 1107 34-35 0.5372916334058218 1107 36-37 0.7695597389956959 1107 38-39 0.597253583404985 1107 40-41 0.5198737645306508 1107 42-43 0.5152513049884888 1107 44-45 0.6018131290258211 1107 46-47 0.5092077716360421 1107 48-49 0.5064829306599279 1107 50-51 0.29546563139305704 1107 52-53 0.3559333527235822 1107 54-55 0.39589639899112683 1107 56-57 0.4666947358979314 1107 58-59 0.44032818914077865 1107 60-61 0.4371272892723894 1107 62-63 0.5162956291787708 1107 64-65 0.5421912279179963 1107 66-67 0.6748044307833254 1107 68-69 0.8409823398801137 1107 70-71 0.6955890253536126 1107 72-73 0.6418049714314265 1107 74-75 0.6804795713266678 1107 76-77 0.7359431749262697 1107 78-79 0.8115533602737806 1107 80-81 0.7756409551721113 1107 82-83 0.8473618463427606 1107 84-85 0.8460909556282488 1107 86-87 1.0532885201577251 1107 88-89 1.0399605834934533 1107 90-91 0.9821488172933535 1107 92-93 1.183860196397056 1107 94-95 1.2917575465889222 1107 96-97 1.3901596368973728 1107 98-99 1.4462134364082537 1107 100-101 1.271579664614471 1108 1 0.03336907641452669 1108 2 0.08247577566429243 1108 3 0.1127778728156521 1108 4 0.07058202546527781 1108 5 -0.022010356085047533 1108 6 0.08835962657163776 1108 7 0.20890172312334698 1108 8 0.10725326579211014 1108 9 0.07721371522897869 1108 10-11 0.2337147533208892 1108 12-13 0.3053194000976873 1108 14-15 0.21497484718294402 1108 16-17 0.23786569948645564 1108 18-19 0.2541499565481544 1108 20-21 0.28811201393065744 1108 22-23 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-1.3418530840565488 2316 96-97 -1.224268059887624 2316 98-99 -1.5618844687615585 2316 100-101 -1.6250742353937646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10243.0 3 2454.0 4 2272.0 5 2504.0 6 2752.0 7 3272.0 8 4023.0 9 5079.0 10 6248.0 11 7505.0 12 9637.0 13 11882.0 14 13617.0 15 16166.0 16 19153.0 17 23028.0 18 21112.0 19 17071.0 20 16947.0 21 18324.0 22 19876.0 23 22357.0 24 24774.0 25 28102.0 26 31900.0 27 36722.0 28 43020.0 29 51181.0 30 61280.0 31 74585.0 32 92312.0 33 116734.0 34 152367.0 35 205635.0 36 281826.0 37 349596.0 38 225687.0 39 19538.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63841475137724 18.86908847372538 14.7340103643571 22.758486410540275 2 14.927846727661192 19.482337131268636 41.56223118877706 24.02758495229311 3 19.458692621305385 24.05490534749604 29.41271973557644 27.073682295622138 4 12.458450883650737 16.31366116851495 37.49521362982176 33.73267431801254 5 13.624997126693966 37.449570422407774 34.2343531980263 14.69107925287196 6 32.026567879133154 36.092221458927185 17.495341240114488 14.385869421825179 7 28.76574787280043 32.61603406450856 20.26843145445973 18.349786608231277 8 26.043390014687446 35.940900380427294 19.28638367358107 18.729325931304192 9 25.954653500660825 17.334812147448194 20.49973493722776 36.21079941466323 10-11 24.883303095320347 27.11306541134529 26.913435890118986 21.090195603215374 12-13 25.718085476716805 24.144293535416853 27.832394321812025 22.305226666054313 14-15 22.466154400135633 25.616608223592763 26.313820984882646 25.603416391388954 16-17 21.878269928131513 29.1324256914749 26.824166398129957 22.16513798226363 18-19 21.817009223192464 27.513109245545053 29.348366978259357 21.321514553003126 20-21 22.425038454501554 26.509459795168254 29.73169908928113 21.333802661049063 22-23 22.062966768136143 26.512967525371035 29.585338173774172 21.838727532718654 24-25 21.819702574079223 26.636421508587187 29.739489717997934 21.804386199335653 26-27 21.69688910341027 26.99520355810202 29.530507578651775 21.77739975983593 28-29 21.552587629762606 27.096234673525448 29.674825150907203 21.676352545804743 30-31 21.709776163216123 26.851862936648107 29.633398460357093 21.804962439778677 32-33 21.446564021975327 26.844279217406303 29.631215009990342 22.07794175062803 34-35 21.94669128740172 26.93226797673213 29.457318507793868 21.66372222807228 36-37 21.90939907309207 26.793520926263092 29.400186905752314 21.89689309489253 38-39 21.694862101827912 27.10962637242398 29.431750406286834 21.76376111946127 40-41 22.00344734686919 26.926456030206907 29.320107675244 21.749988947679906 42-43 21.73483328972995 26.93821925735499 29.269033854057884 22.057913598857176 44-45 21.90716385912077 27.167782421463144 29.032718275741104 21.892335443674984 46-47 21.946663070611187 27.36549360014501 28.608893299268523 22.07895002997527 48-49 22.068283511717876 27.392996146972052 28.501462352604335 22.037257988705733 50-51 22.11976635697818 27.592894941993567 28.342616495975204 21.944722205053047 52-53 22.202795707595524 27.60644569311727 28.20566203484799 21.985096564439218 54-55 22.08901746331814 27.583033875041192 28.284830087393537 22.043118574247135 56-57 22.06376855202382 27.861959851818273 28.17638685903197 21.89788473712593 58-59 22.136216029277005 27.58608814754172 28.225716635958115 22.051979187223157 60-61 22.11601700054192 27.546460748095157 28.4372723425175 21.900249908845424 62-63 22.084615786668806 27.63866249377804 28.423936697403214 21.85278502214995 64-65 21.96320627603914 27.576634053451937 28.420095263052875 22.040064407456043 66-67 21.843756090341472 27.68301063987234 28.30360880359928 22.169624466186914 68-69 21.994699254373508 27.58043750813663 28.35361556917556 22.071247668314307 70-71 21.98474356334733 27.664228460168673 28.205797035611784 22.14523094087221 72-73 22.030012663004122 27.604909386863934 28.145840685487368 22.219237264644576 74-75 21.91808390417274 27.83603201341146 28.075236255098112 22.17064782731769 76-77 22.031665716950805 27.680842712642523 28.091960176903612 22.195531393503064 78-79 22.056661159544046 27.738091865058234 28.01177892436223 22.19346805103549 80-81 21.90083694230305 27.939604750651377 27.972185614205454 22.187372692840125 82-83 22.012149909781765 27.910942343368543 27.99068314789831 22.08622459895138 84-85 22.087365481764913 27.83724618406085 27.826476422035956 22.24891191213828 86-87 22.006341537510263 27.816535959555605 27.98144816999462 22.19567433293951 88-89 22.125263819604783 27.67493907910168 27.934497029541443 22.265300071752094 90-91 22.08532977202376 27.72089213149556 27.963664286652858 22.230113809827827 92-93 22.117611202257724 27.581149111568827 28.022177688188716 22.279061997984734 94-95 22.034691008629036 27.57246953754114 28.005704170521383 22.38713528330844 96-97 22.112181318060028 27.50364562052967 27.897331120032803 22.486841941377495 98-99 22.02444395163378 27.407222693865908 27.881354855916378 22.68697849858393 100-101 22.3884179507991 26.88385816966078 28.356276304081778 22.371447575458348 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2572.0 1 1465.5 2 402.0 3 450.0 4 387.0 5 349.0 6 545.5 7 1148.5 8 1944.0 9 2348.5 10 2094.5 11 1885.5 12 2019.5 13 2140.0 14 2323.5 15 2496.0 16 2666.5 17 2864.0 18 3075.0 19 3357.0 20 3823.0 21 4597.0 22 5690.5 23 6943.5 24 8523.5 25 10755.5 26 13533.5 27 16803.0 28 19541.0 29 22326.5 30 26343.0 31 30257.5 32 33850.5 33 37499.0 34 41530.5 35 45649.5 36 50169.5 37 54295.5 38 57432.5 39 60582.5 40 63684.5 41 66816.0 42 69329.0 43 72061.0 44 75366.5 45 77524.5 46 78195.0 47 79376.5 48 81608.5 49 80048.0 50 76247.0 51 73035.0 52 69007.0 53 66142.0 54 63641.0 55 59170.0 56 53522.0 57 47786.5 58 42742.5 59 37529.0 60 31131.0 61 25253.0 62 19856.5 63 14693.5 64 10824.0 65 8320.0 66 6161.5 67 4432.5 68 3235.0 69 2335.5 70 1715.5 71 1213.5 72 882.5 73 681.5 74 511.5 75 363.5 76 277.5 77 199.0 78 127.0 79 89.0 80 64.5 81 46.5 82 32.0 83 25.0 84 21.5 85 17.5 86 12.0 87 7.5 88 7.5 89 4.5 90 1.5 91 2.5 92 3.0 93 2.5 94 1.0 95 1.0 96 1.5 97 0.5 98 0.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3249487391413532 2 0.28779223565610246 3 0.2869145229753485 4 0.2884749010744666 5 0.2975445987755908 6 0.20006972939630432 7 0.19894820763756318 8 0.20182515475781226 9 0.19992344394951203 10-11 0.20694514539554365 12-13 0.1970221159214642 14-15 0.1981680185880041 16-17 0.2047752446014575 18-19 0.19711963955265913 20-21 0.18946403450386073 22-23 0.1884156554685157 24-25 0.19290174250348036 26-27 0.18778175186574897 28-29 0.20299543833215086 30-31 0.20828609532447334 32-33 0.21006590159377997 34-35 0.20828609532447334 36-37 0.1835394739087715 38-39 0.17485987073242684 40-41 0.18058938406512628 42-43 0.1914145071277584 44-45 0.19694897319806806 46-47 0.19982592031831714 48-49 0.1990944930843555 50-51 0.20277601016196237 52-53 0.22491387444320105 54-55 0.1899028908442377 56-57 0.17403091986727032 58-59 0.17461606165443963 60-61 0.16766750293180416 62-63 0.1756400597819859 64-65 0.1709833063924302 66-67 0.16998368917268267 68-69 0.18112576403669814 70-71 0.17473796619343326 72-73 0.16981302281809163 74-75 0.17459168074664091 76-77 0.17951662412198255 78-79 0.18232042851883545 80-81 0.17412844349846524 82-83 0.17198292361217776 84-85 0.16510750761293846 86-87 0.1624743695706766 88-89 0.1686183583359543 90-91 0.17544501251959616 92-93 0.16637531481847195 94-95 0.1712027345626187 96-97 0.1689840719529351 98-99 0.17412844349846524 100-101 0.15111286653647263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.850936520967046 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2737204775124 49.82427573513017 2 12.361311028834681 15.538399495784368 3 3.770043233891887 7.108522439239209 4 1.6196946849959228 4.071973113201058 5 0.8182126930647861 2.571271701623218 6 0.49564493849051344 1.8691049139603524 7 0.333908498763256 1.4690523301706566 8 0.22682307238557423 1.1404834019197152 9 0.16616470745367098 0.9399246732176162 >10 0.8649767210715873 9.621805043651111 >50 0.046879227382615275 1.998375685535083 >100 0.02145554920040861 2.4355363793549087 >500 7.767779685267959E-4 0.30347272086738264 >1k 2.3303339055802622E-4 0.41893491276751804 >5k 1.5535559370535078E-4 0.6888674535776342 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7929 0.38663243587211726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6181 0.3013967822077887 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.2027028674385662 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2811 0.13706946364440933 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.8761815597441956E-5 0.0 0.0 0.0 0.0 7 4.8761815597441956E-5 0.0 0.0 0.0 0.0 8 4.8761815597441956E-5 0.0 0.0 0.0 0.0 9 4.8761815597441956E-5 0.0 0.0 0.0 0.0 10-11 9.752363119488391E-5 0.0 0.0 0.0 0.0 12-13 1.2190453899360489E-4 0.0 0.0 0.0 0.0 14-15 1.4628544679232586E-4 0.0 0.0 0.0 0.0 16-17 1.7066635459104683E-4 0.0 0.0 0.0 2.4380907798720978E-5 18-19 1.9504726238976782E-4 0.0 0.0 0.0 4.8761815597441956E-5 20-21 1.9504726238976782E-4 0.0 0.0 0.0 4.8761815597441956E-5 22-23 1.9504726238976782E-4 0.0 0.0 9.752363119488391E-5 4.8761815597441956E-5 24-25 2.4380907798720979E-4 0.0 0.0 1.9504726238976782E-4 4.8761815597441956E-5 26-27 2.4380907798720979E-4 0.0 0.0 3.9009452477953565E-4 9.752363119488391E-5 28-29 2.4380907798720979E-4 0.0 0.0 9.020935885526762E-4 9.752363119488391E-5 30-31 2.4380907798720979E-4 0.0 0.0 0.001974853531696399 9.752363119488391E-5 32-33 2.4380907798720979E-4 0.0 0.0 0.00324266073722989 9.752363119488391E-5 34-35 2.925708935846517E-4 0.0 0.0 0.004364182495971055 9.752363119488391E-5 36-37 2.925708935846517E-4 0.0 0.0 0.006314655119868734 9.752363119488391E-5 38-39 2.925708935846517E-4 0.0 0.0 0.010605694892443625 9.752363119488391E-5 40-41 2.925708935846517E-4 0.0 0.0 0.015238067374200612 9.752363119488391E-5 42-43 3.9009452477953565E-4 0.0 0.0 0.018919584451807477 9.752363119488391E-5 44-45 3.9009452477953565E-4 0.0 0.0 0.021040723430296206 9.752363119488391E-5 46-47 3.9009452477953565E-4 2.4380907798720978E-5 0.0 0.024332145983123533 9.752363119488391E-5 48-49 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.029110803911672846 9.752363119488391E-5 50-51 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.03388946184022216 9.752363119488391E-5 52-53 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.04332487315832718 1.2190453899360489E-4 54-55 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.05098047820712556 1.4628544679232586E-4 56-57 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.06751073369465839 1.4628544679232586E-4 58-59 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.0937933523016796 1.4628544679232586E-4 60-61 3.9009452477953565E-4 4.8761815597441956E-5 0.0 0.11905197278115454 1.4628544679232586E-4 62-63 4.388563403769776E-4 4.8761815597441956E-5 0.0 0.1314618548507035 1.9504726238976782E-4 64-65 4.388563403769776E-4 7.314272339616293E-5 0.0 0.15489190724527435 1.9504726238976782E-4 66-67 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.20021601484309667 1.9504726238976782E-4 68-69 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.2612901888788927 1.9504726238976782E-4 70-71 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.32507064368034677 1.9504726238976782E-4 72-73 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.3945806118145003 1.9504726238976782E-4 74-75 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.47450122757870766 2.4380907798720979E-4 76-77 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.5397689177558838 2.4380907798720979E-4 78-79 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.6203965798462541 2.4380907798720979E-4 80-81 5.363799715718615E-4 9.752363119488391E-5 0.0 0.7288428577349649 2.6818998578593075E-4 82-83 5.363799715718615E-4 9.752363119488391E-5 0.0 0.8273661061495964 2.925708935846517E-4 84-85 5.363799715718615E-4 9.752363119488391E-5 0.0 0.9419076109879876 2.925708935846517E-4 86-87 5.363799715718615E-4 9.752363119488391E-5 0.0 1.092167145751505 2.925708935846517E-4 88-89 5.363799715718615E-4 9.752363119488391E-5 0.0 1.2877507881128447 2.925708935846517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4860 0.0 44.02616 1 GTATCAA 8385 0.0 40.431614 1 ATCAACG 10485 0.0 32.050655 3 TATCAAC 10540 0.0 31.884186 2 TCAACGC 10605 0.0 31.73591 4 CAACGCA 10675 0.0 31.527803 5 AACGCAG 10960 0.0 30.769886 6 ACGCAGA 12450 0.0 26.897942 7 CGCAGAG 12430 0.0 26.82658 8 GCAGAGT 13525 0.0 24.02191 9 GTACATG 8065 0.0 23.759388 1 GAGTACT 7395 0.0 23.1246 12-13 TACATGG 8015 0.0 23.01043 2 ACATGGG 8085 0.0 21.69363 3 GTGTAGC 1365 0.0 21.24867 1 GTACTTT 7975 0.0 21.233822 14-15 AGAGTAC 12105 0.0 20.46392 10-11 CAGAGTA 13120 0.0 20.401373 10-11 ATACCGT 260 1.4924808E-8 20.095436 6 AGTACTT 7855 0.0 20.016663 12-13 >>END_MODULE