FastQCFastQC Report
Thu 26 May 2016
SRR937583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050785
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101940.4970779482003233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85910.4189127577976238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58990.2876459502093101No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.10732475612996975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56700.045.757771
GTATCAA91000.035.8210261
ATCAACG113350.028.585383
TATCAAC113950.028.4348642
TCAACGC114950.028.1874984
CAACGCA116800.027.7410345
AACGCAG119150.027.3061926
GGACCGA5000.024.698356
ACGCAGA136700.023.8022757
CGCAGAG138050.023.4662868
GTACATG80900.022.3198031
GAGTACT91050.022.04160312-13
TACATGG78850.021.9923842
GCAGAGT149500.021.510179
CGTACAC2457.510607E-921.3308283
ACATGGG78400.021.3308283
GTACTTT97700.020.32106214-15
GTGTAGC12450.020.2284131
AGTACTT96200.020.1703512-13
CAGAGTA146550.019.59326710-11
Produced by FastQC (version 0.11.5)