##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937583_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050785 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.152494776390505 33.0 31.0 34.0 31.0 34.0 2 32.4403967261317 34.0 31.0 34.0 31.0 34.0 3 32.5377253100642 34.0 31.0 34.0 31.0 34.0 4 36.165742874070176 37.0 35.0 37.0 35.0 37.0 5 36.08232554850947 37.0 35.0 37.0 35.0 37.0 6 36.120418278854196 37.0 35.0 37.0 35.0 37.0 7 36.06902381283265 37.0 35.0 37.0 35.0 37.0 8 36.04342385964399 37.0 35.0 37.0 35.0 37.0 9 37.75115626455236 39.0 37.0 39.0 35.0 39.0 10-11 37.66265405686116 39.0 37.0 39.0 35.0 39.0 12-13 37.55603610324827 39.0 37.0 39.0 35.0 39.0 14-15 38.79546661400391 40.0 38.0 41.0 35.0 41.0 16-17 38.751197468286534 40.0 38.0 41.0 35.0 41.0 18-19 38.72266912426217 40.0 38.0 41.0 35.0 41.0 20-21 38.6239303486226 40.0 38.0 41.0 34.0 41.0 22-23 38.56999075963594 40.0 38.0 41.0 34.0 41.0 24-25 38.60785235897474 40.0 38.0 41.0 34.0 41.0 26-27 38.48231872185529 40.0 38.0 41.0 34.0 41.0 28-29 38.334789361147074 40.0 38.0 41.0 34.0 41.0 30-31 38.367993475669074 40.0 38.0 41.0 34.0 41.0 32-33 38.28891863359641 40.0 38.0 41.0 34.0 41.0 34-35 38.14667919845328 40.0 38.0 41.0 34.0 41.0 36-37 38.00142408882452 40.0 37.0 41.0 33.0 41.0 38-39 37.86839161589343 40.0 37.0 41.0 33.0 41.0 40-41 37.69050022308531 40.0 37.0 41.0 33.0 41.0 42-43 37.47399239803295 40.0 37.0 41.0 33.0 41.0 44-45 37.185656711942016 40.0 37.0 41.0 32.0 41.0 46-47 36.83181732848641 40.0 36.0 41.0 31.0 41.0 48-49 36.50710532795978 39.0 36.0 41.0 30.5 41.0 50-51 36.251688255960524 39.0 36.0 41.0 30.0 41.0 52-53 36.01189788300577 39.0 35.0 40.5 29.5 41.0 54-55 35.78723245001304 39.0 35.0 40.0 29.0 41.0 56-57 35.51221532242532 38.5 35.0 40.0 28.0 41.0 58-59 35.246778428748016 38.0 34.5 40.0 28.0 41.0 60-61 34.91526196066384 38.0 34.0 40.0 27.0 41.0 62-63 34.537707024383344 37.5 34.0 40.0 26.0 41.0 64-65 34.1166092496288 37.0 33.5 39.5 26.0 41.0 66-67 33.68889157078875 36.0 33.0 39.0 25.5 40.5 68-69 33.23682443552103 36.0 32.5 39.0 24.0 40.0 70-71 32.83333723427858 35.0 32.0 38.5 23.5 40.0 72-73 32.33118537535627 35.0 31.5 37.5 22.0 39.5 74-75 31.847029064480186 35.0 31.0 37.0 20.5 39.0 76-77 30.63898946013356 33.5 29.5 36.0 20.0 38.5 78-79 31.22380893170176 34.0 31.0 36.0 20.0 38.5 80-81 31.023052879750924 34.0 31.0 36.0 19.5 37.5 82-83 30.63269430973993 34.0 31.0 35.0 18.0 37.0 84-85 30.23692439724301 34.0 30.0 35.0 16.0 36.5 86-87 29.835620993912087 34.0 30.0 35.0 9.0 36.0 88-89 29.376384652706157 34.0 29.5 35.0 6.0 36.0 90-91 28.800796524257784 34.0 29.0 35.0 2.0 35.0 92-93 28.329713012334302 33.5 29.0 35.0 2.0 35.0 94-95 27.829340472063137 33.0 27.0 35.0 2.0 35.0 96-97 27.218835957938058 33.0 26.5 35.0 2.0 35.0 98-99 26.25796658352777 32.5 25.0 34.0 2.0 35.0 100-101 23.387164427280283 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36773381093840385 1101 2 0.27458862178116306 1101 3 0.24925208707252722 1101 4 0.1325472450352123 1101 5 0.09597962396642856 1101 6 0.18709505684980599 1101 7 0.1840797016742428 1101 8 0.15961164384123805 1101 9 0.1079043652904943 1101 10-11 0.22892168565209658 1101 12-13 0.3038753268516672 1101 14-15 0.320676813886557 1101 16-17 0.24013284601388563 1101 18-19 0.3064178683752772 1101 20-21 0.30251728811055045 1101 22-23 0.2633035667146757 1101 24-25 0.17501506945501433 1101 26-27 0.17025181689683677 1101 28-29 0.07968928363329297 1101 30-31 0.15651252272142813 1101 32-33 0.2595405993224844 1101 34-35 0.26449837176836866 1101 36-37 0.12500890162795741 1101 38-39 0.1823555641089527 1101 40-41 0.26660658879089993 1101 42-43 0.25785538674239916 1101 44-45 0.26801282645241287 1101 46-47 0.07859868949761761 1101 48-49 -0.06862531673539962 1101 50-51 0.07473527520763668 1101 52-53 0.13221679481451076 1101 54-55 0.06574826784970611 1101 56-57 -0.027402630319564025 1101 58-59 0.006822966728385893 1101 60-61 -0.10366226780276122 1101 62-63 -0.1317225232443633 1101 64-65 -0.27217809886803224 1101 66-67 -0.1983184097911206 1101 68-69 -0.19175752339425145 1101 70-71 -0.1343230760403813 1101 72-73 -0.22512049072079776 1101 74-75 -0.2888276583972278 1101 76-77 -0.21957496771603857 1101 78-79 -0.20500172201709788 1101 80-81 -0.1352127466414892 1101 82-83 -0.1434753312022039 1101 84-85 -0.035500687900157146 1101 86-87 0.07102669031029407 1101 88-89 -0.12428988747158343 1101 90-91 -0.2361981188648059 1101 92-93 -0.38365635824072797 1101 94-95 -0.34806977992919386 1101 96-97 -0.3655410365199714 1101 98-99 -0.2650948179603816 1101 100-101 -0.13171234825847122 1102 1 0.34422256275807683 1102 2 0.2594647342179428 1102 3 0.09698613026434089 1102 4 0.08520020978240694 1102 5 0.055141188056289536 1102 6 0.09907748297499808 1102 7 0.04383856740143699 1102 8 0.10988778329166138 1102 9 0.11982976261614198 1102 10-11 0.1213112486314003 1102 12-13 0.24007013648052578 1102 14-15 0.2790523560353435 1102 16-17 0.1608731323584891 1102 18-19 0.22763699028184448 1102 20-21 0.21580302865952206 1102 22-23 0.18350920806354765 1102 24-25 0.17709033621473225 1102 26-27 0.2205281439419977 1102 28-29 0.18574362232433117 1102 30-31 0.2134939545945187 1102 32-33 0.3267947189028675 1102 34-35 0.26501847817210944 1102 36-37 0.317956652742275 1102 38-39 0.29390126969027364 1102 40-41 0.265105830908162 1102 42-43 0.42217946415149044 1102 44-45 0.39510216281728106 1102 46-47 0.23617826718434998 1102 48-49 0.30438758603049365 1102 50-51 0.3239201760825736 1102 52-53 0.36883613132945214 1102 54-55 0.3179970597396107 1102 56-57 0.4701830254365973 1102 58-59 0.33121694193587103 1102 60-61 0.167125104820407 1102 62-63 0.21317086666754648 1102 64-65 0.2705766172636146 1102 66-67 0.24390100476322374 1102 68-69 0.23567473614681234 1102 70-71 0.14757740870441438 1102 72-73 0.0691743781880021 1102 74-75 0.12891064319507706 1102 76-77 0.09532999679103682 1102 78-79 0.13632127109442038 1102 80-81 0.20882802519828658 1102 82-83 0.14750333177259733 1102 84-85 0.2565083391585077 1102 86-87 0.16127664153566457 1102 88-89 0.17175148842324361 1102 90-91 0.14825806275691633 1102 92-93 0.1576360569289328 1102 94-95 0.03926316695898535 1102 96-97 0.19941364059197042 1102 98-99 0.47530830436639704 1102 100-101 0.5590968228729487 1103 1 0.24972515747319335 1103 2 0.16884203820742982 1103 3 0.16695907007478183 1103 4 0.10046976096970894 1103 5 0.06523533329622921 1103 6 0.06485422349992831 1103 7 0.04617171849328372 1103 8 0.0907292759384859 1103 9 0.15852387473205454 1103 10-11 0.12539153609804998 1103 12-13 0.2656111399247152 1103 14-15 0.2116540966493048 1103 16-17 0.1343634515957035 1103 18-19 0.16640017756080283 1103 20-21 0.16265088165297925 1103 22-23 0.13059870169340826 1103 24-25 0.1514366932845661 1103 26-27 0.11568059458542024 1103 28-29 0.08791099747703157 1103 30-31 0.10169002654844661 1103 32-33 0.14767645456604583 1103 34-35 0.17998112015128243 1103 36-37 0.2494547044798594 1103 38-39 0.21179242700087997 1103 40-41 0.15996199072979067 1103 42-43 0.1569207094366547 1103 44-45 0.2447007343883172 1103 46-47 0.15270261334107005 1103 48-49 0.014682723744506632 1103 50-51 -3.1169385755447365E-4 1103 52-53 0.019037658061961338 1103 54-55 -0.20409196641411853 1103 56-57 -0.16062658979047484 1103 58-59 -0.12315972539565223 1103 60-61 -0.18081114390182051 1103 62-63 -0.21947773286301953 1103 64-65 -0.2159191104497964 1103 66-67 -0.14675443683697864 1103 68-69 -0.23075213010692863 1103 70-71 -0.26483715446807565 1103 72-73 -0.19851536369394296 1103 74-75 -0.2026112943465499 1103 76-77 -0.12468185484183536 1103 78-79 -0.11276069483413664 1103 80-81 -0.11630752061793359 1103 82-83 -0.09749812408162484 1103 84-85 -0.12705916261345251 1103 86-87 -0.3068592951796454 1103 88-89 -0.29673171627253225 1103 90-91 -0.18076599400982474 1103 92-93 -0.10980236088252937 1103 94-95 -0.20320011613031141 1103 96-97 -0.19059367874853805 1103 98-99 -0.21707893593948313 1103 100-101 -0.18168298963795948 1104 1 0.2878856613032994 1104 2 0.2015471156351083 1104 3 0.17675514087932243 1104 4 0.09434262716681019 1104 5 0.038210711007749865 1104 6 0.06366217938077767 1104 7 0.11508502341308002 1104 8 0.12594075452144438 1104 9 0.08967904702545582 1104 10-11 0.08079034490990011 1104 12-13 0.11559603664463225 1104 14-15 0.1569874308836532 1104 16-17 0.19408763258908834 1104 18-19 0.16670798713917634 1104 20-21 0.024658597503176338 1104 22-23 0.1387313844031226 1104 24-25 0.09036909749023891 1104 26-27 0.07354401993726611 1104 28-29 0.10262379976507674 1104 30-31 0.2299843380066946 1104 32-33 0.13909461539874712 1104 34-35 0.22853427998047948 1104 36-37 0.27084775174513 1104 38-39 0.11388409050836401 1104 40-41 0.19025572418629366 1104 42-43 0.14524928653197122 1104 44-45 0.003982043510454503 1104 46-47 -0.018386014418375396 1104 48-49 -0.06472584036716711 1104 50-51 0.061932065649571655 1104 52-53 0.06138698510439866 1104 54-55 0.10782815942176427 1104 56-57 0.23174277359444062 1104 58-59 0.11352432862656059 1104 60-61 0.0855655533025299 1104 62-63 0.06857267807909295 1104 64-65 -0.1776713206982663 1104 66-67 -0.0673026239590726 1104 68-69 -0.006798981134977566 1104 70-71 -0.021653621796225764 1104 72-73 0.00541878735642598 1104 74-75 -0.1115741055884385 1104 76-77 -0.07122829444346834 1104 78-79 0.009295432603760645 1104 80-81 -0.04255035388064954 1104 82-83 -0.011399966952101437 1104 84-85 0.15187420567930232 1104 86-87 0.0965686876280678 1104 88-89 -0.016528591233818446 1104 90-91 -0.04827475019558136 1104 92-93 0.15427775925893883 1104 94-95 0.060339507060309217 1104 96-97 0.15060000521966188 1104 98-99 0.16893263355598975 1104 100-101 0.20655690496119306 1105 1 0.2441406730958846 1105 2 0.21019833657464204 1105 3 0.20847567992495186 1105 4 0.10210764758568303 1105 5 0.07519564378569754 1105 6 0.14513369834660494 1105 7 0.18797467626271924 1105 8 0.08868248214556473 1105 9 0.11597449766635037 1105 10-11 0.18858553241043552 1105 12-13 0.22371034699250458 1105 14-15 0.2607763574115083 1105 16-17 0.19610674188277244 1105 18-19 0.22150713693064006 1105 20-21 0.10604512281292244 1105 22-23 0.07797950559001521 1105 24-25 0.04222393104546995 1105 26-27 0.05368870860436914 1105 28-29 0.1217964420523856 1105 30-31 0.06102261597629166 1105 32-33 0.13438290708065637 1105 34-35 0.1839071709478972 1105 36-37 0.1253999213045276 1105 38-39 0.08260891822055072 1105 40-41 0.09747392941945776 1105 42-43 0.08902360321697955 1105 44-45 0.04251363417919407 1105 46-47 -0.11850162845560419 1105 48-49 -0.06896543725699189 1105 50-51 -5.09373721392592E-4 1105 52-53 0.16446117164711893 1105 54-55 0.14946405838493604 1105 56-57 0.05210494176220237 1105 58-59 0.046388972942843054 1105 60-61 0.09218531253702622 1105 62-63 3.453239021453669E-4 1105 64-65 -0.014646688856785772 1105 66-67 0.08472394211135992 1105 68-69 0.18843609318574295 1105 70-71 -0.027146818450695775 1105 72-73 0.1355739925719135 1105 74-75 -0.027899324407439252 1105 76-77 -0.017742693275408783 1105 78-79 0.23643451894235312 1105 80-81 0.13800892250829477 1105 82-83 0.06866235719602543 1105 84-85 0.039275403387240004 1105 86-87 0.09654976237398571 1105 88-89 0.18893756610553325 1105 90-91 0.3978909479967072 1105 92-93 0.48467371183837216 1105 94-95 0.09145013162995852 1105 96-97 0.26724677203831604 1105 98-99 0.34009875105031284 1105 100-101 0.38832955078424547 1106 1 0.13559040544171097 1106 2 0.1468794247905265 1106 3 0.12732977784482813 1106 4 0.026396469662877564 1106 5 -0.0283593903127084 1106 6 -0.08845384534580347 1106 7 0.041868738984241816 1106 8 -0.01843687450668341 1106 9 0.1027746247715271 1106 10-11 -8.395610852005575E-4 1106 12-13 -0.2407070468624397 1106 14-15 -0.1541281914873096 1106 16-17 -0.018258832168214667 1106 18-19 -0.016758855100846404 1106 20-21 0.0794879621470912 1106 22-23 0.09945050361010743 1106 24-25 0.05643991207746524 1106 26-27 0.09021727060480345 1106 28-29 0.10265489088033775 1106 30-31 0.06222033786038139 1106 32-33 0.10356332310568206 1106 34-35 -0.13875128276870896 1106 36-37 0.07511637898094392 1106 38-39 0.17361304913889342 1106 40-41 0.009189116044076684 1106 42-43 -0.1575821996233273 1106 44-45 -0.06182211904121715 1106 46-47 -0.1096179541381801 1106 48-49 -0.07579098539807916 1106 50-51 0.07825746160407476 1106 52-53 0.09197113229340204 1106 54-55 0.05022546729696131 1106 56-57 0.005469318091307684 1106 58-59 -0.014172734177485324 1106 60-61 -0.07080337021332639 1106 62-63 -0.29553720157999663 1106 64-65 -0.38614017471633844 1106 66-67 -0.4125215419882622 1106 68-69 -0.05769257636523406 1106 70-71 -0.21593907481206287 1106 72-73 -0.2159104492652446 1106 74-75 -0.002025109648570833 1106 76-77 0.012880122104316882 1106 78-79 -0.09467545148124756 1106 80-81 -0.32419708565576855 1106 82-83 -0.41375630771072736 1106 84-85 -0.5121811730836363 1106 86-87 -0.22241335268064333 1106 88-89 -0.2285897492864173 1106 90-91 -0.4778632501407145 1106 92-93 -0.4078126610197863 1106 94-95 -0.39675548544124695 1106 96-97 -0.35874232624820834 1106 98-99 -0.5300625747544814 1106 100-101 -0.8303734875184148 1107 1 0.23899421410867205 1107 2 0.24352209985425333 1107 3 0.19098849729302003 1107 4 0.08516540053707189 1107 5 0.052861182486807934 1107 6 0.09994771410838865 1107 7 0.04559643429088567 1107 8 0.07111028398784924 1107 9 0.19226780215944217 1107 10-11 0.1316060829393919 1107 12-13 0.1538041242276762 1107 14-15 0.1840057116465843 1107 16-17 0.16304871019195843 1107 18-19 0.2527605631027825 1107 20-21 0.1538686788962309 1107 22-23 0.2642840618647213 1107 24-25 0.22833824766001243 1107 26-27 0.2243745655515781 1107 28-29 0.2744984956186727 1107 30-31 0.21209288246976854 1107 32-33 0.2814295699193039 1107 34-35 0.23808482459371305 1107 36-37 0.3874533110547205 1107 38-39 0.33796977428509223 1107 40-41 0.26521025864416714 1107 42-43 0.40328674624561245 1107 44-45 0.37170164764044245 1107 46-47 0.2149794367749962 1107 48-49 0.2525131081691754 1107 50-51 0.4014925793128725 1107 52-53 0.34917761002619585 1107 54-55 0.2303386776733376 1107 56-57 0.12985303379081614 1107 58-59 0.1499913084075999 1107 60-61 0.23778787285159808 1107 62-63 0.31597127045478857 1107 64-65 0.44862590715500517 1107 66-67 0.2853327090238835 1107 68-69 0.3232460950997549 1107 70-71 0.3723755150814725 1107 72-73 0.4408326906357871 1107 74-75 0.2995890753866668 1107 76-77 0.1510770031959332 1107 78-79 0.23497067237540037 1107 80-81 0.24794491464412616 1107 82-83 0.13543322595249307 1107 84-85 0.47157726146427237 1107 86-87 0.382628632624499 1107 88-89 0.2636827053544586 1107 90-91 0.3063877620050377 1107 92-93 0.3660912226209412 1107 94-95 0.5021304044972759 1107 96-97 0.6294470574674911 1107 98-99 0.38846793956550485 1107 100-101 0.10819526341875729 1108 1 0.22134343791364586 1108 2 0.1307504411395044 1108 3 0.08777680062289761 1108 4 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100-101 0.3105763138539217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 14.0 5 43.0 6 68.0 7 170.0 8 433.0 9 822.0 10 1492.0 11 2459.0 12 3864.0 13 5731.0 14 7665.0 15 10635.0 16 14285.0 17 20066.0 18 18549.0 19 11441.0 20 9558.0 21 9712.0 22 10466.0 23 11419.0 24 13151.0 25 15874.0 26 19191.0 27 23525.0 28 29749.0 29 37664.0 30 47019.0 31 59828.0 32 76326.0 33 100768.0 34 139371.0 35 200995.0 36 309921.0 37 463031.0 38 347120.0 39 28346.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59170750358949 19.534127442412135 15.434210937012297 22.43995411698608 2 15.501722511135979 20.07031453809151 40.77321610992864 23.65474684084387 3 19.69333694170769 23.98798508863679 29.98568840712215 26.33298956253337 4 12.960695538537681 16.95136252703233 37.08838322886115 32.999558705568845 5 13.587920460953399 37.40465909467847 34.34994526861306 14.657475175755069 6 32.06167394436765 36.49168489139525 17.08516494903171 14.361476215205396 7 28.664438251693863 32.854541065982055 20.15325838642276 18.327762295901326 8 26.288713833970895 35.94799064748377 19.124189030054346 18.63910648849099 9 25.82849981836224 17.176154496936537 20.46070163376463 36.53464405093659 10-11 25.000865522226857 27.160501954129764 26.81373230250855 21.024900221134835 12-13 25.8290008833541 24.159771278825126 27.87902608198687 22.132201755833904 14-15 22.460911309571703 25.625553141845685 26.447336020109375 25.466199528473243 16-17 21.73123950097158 29.09854031505009 27.115470417425524 22.05474976655281 18-19 21.66641066713478 27.183151817474773 29.95899131308255 21.191446202307898 20-21 22.180823440779996 26.21679015596467 30.495785760086992 21.106600643168345 22-23 21.930418903120653 26.173210339030778 30.251746597928342 21.644624159920227 24-25 21.75782744285532 26.296414318151395 30.482062569805706 21.463695669187576 26-27 21.63502599642816 26.64760058758632 30.296584806870612 21.4207886091149 28-29 21.462388011521533 26.729387854019638 30.38209081568914 21.42613331876969 30-31 21.664070099986102 26.43807127514586 30.38185377794357 21.51600484692447 32-33 21.430086527841777 26.458965713129363 30.32192550657431 21.789022252454547 34-35 21.880621323054342 26.590159377994283 30.17878519688802 21.350434102063357 36-37 21.86389602030442 26.422930731402854 30.12695138690794 21.586221861384786 38-39 21.671457515049113 26.66742247480843 30.163571510421622 21.49754849972084 40-41 21.911065686482946 26.57645511056324 30.030300784290016 21.4821784186638 42-43 21.71611546237105 26.545831117470364 29.950786506388148 21.787266913770438 44-45 21.864993161155365 26.803443559417488 29.67904972973764 21.65251354968951 46-47 21.944791872380577 27.098647591044404 29.17936302440285 21.77719751217217 48-49 22.06691583954437 27.1388273270967 29.027957586972793 21.76629924638614 50-51 22.146738931677383 27.30747006634045 28.802434189834624 21.743356812147542 52-53 22.30211845707863 27.439980300226495 28.57659384089507 21.6813074017998 54-55 22.12857775020023 27.43430861146904 28.647699109973875 21.78941452835686 56-57 22.09361293358397 27.60511218874724 28.612726346252774 21.688548531416018 58-59 22.26059777109741 27.282211445860977 28.632377357938545 21.82481342510307 60-61 22.097830830633146 27.36069358806506 28.852927049885775 21.688548531416018 62-63 22.063575655175946 27.393778479947922 28.861362843984136 21.681283020892 64-65 22.006719378189327 27.332826210451124 28.794071538459665 21.86638287289989 66-67 21.924482576184243 27.427058419093175 28.684381834273214 21.964077170449364 68-69 22.054603481106017 27.325511938111504 28.72895013372928 21.8909344470532 70-71 22.01603288496844 27.453048466806614 28.55274931306792 21.978169335157023 72-73 22.18543143235395 27.357621593682417 28.474827931743214 21.98211904222042 74-75 22.02188430284013 27.602381527073778 28.31810745641303 22.057626713673056 76-77 22.088029705698062 27.367349575894107 28.533171444105548 22.01144927430228 78-79 22.058943282694187 27.490156208476268 28.42799220786187 22.02290830096768 80-81 21.98909198185085 27.702904009927902 28.36730812835085 21.940695879870393 82-83 22.018285322516586 27.701173778343314 28.360852664888636 21.91968823425147 84-85 22.060094563812044 27.596829408453203 28.297512488513544 22.04556353922121 86-87 22.012473272429826 27.579195283757198 28.38869018449033 22.01964125932265 88-89 22.07913067435153 27.539820117662263 28.322642305263596 22.058406902722616 90-91 22.04765746976938 27.53458437666192 28.337596868319558 22.080161285249144 92-93 22.060099607679255 27.33557138123673 28.46060127890077 22.143727732183248 94-95 21.907952320696708 27.36508215146883 28.448862264937574 22.278103262896888 96-97 21.995065304261537 27.28774591193128 28.36294394585488 22.354244837952297 98-99 21.901235687630106 27.252054237426986 28.21881730733128 22.62789276761163 100-101 21.88185536299711 27.333127845173777 28.527235147058967 22.257781644770148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 388.0 1 280.5 2 282.0 3 420.0 4 341.0 5 287.0 6 603.5 7 1441.5 8 2403.0 9 2871.5 10 2625.5 11 2247.0 12 2303.5 13 2545.5 14 2782.5 15 2943.5 16 3103.0 17 3354.5 18 3548.0 19 3791.0 20 4366.5 21 4983.0 22 5856.5 23 7209.0 24 8817.5 25 10939.0 26 13696.5 27 16916.0 28 19594.0 29 22474.5 30 26318.0 31 30083.0 32 33877.0 33 37606.0 34 41477.5 35 45442.5 36 50094.5 37 54453.5 38 57600.5 39 60313.0 40 63250.5 41 66659.5 42 69668.5 43 72792.5 44 76129.0 45 78009.0 46 78343.5 47 79983.0 48 82103.5 49 80134.5 50 76890.5 51 73690.5 52 69118.0 53 66254.0 54 64275.5 55 59269.0 56 52549.0 57 46980.5 58 42282.5 59 36805.0 60 30120.0 61 24173.0 62 18888.0 63 13983.0 64 10161.5 65 7474.0 66 5504.5 67 4265.0 68 3084.0 69 2046.5 70 1470.0 71 989.0 72 733.5 73 609.5 74 467.5 75 364.0 76 254.0 77 152.5 78 106.0 79 82.0 80 52.5 81 36.0 82 30.0 83 23.5 84 14.5 85 7.5 86 5.0 87 3.0 88 5.0 89 3.5 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01721292090589701 2 0.0 3 0.0 4 0.0 5 0.03769288345682263 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038278025243991937 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.095226949680245E-4 24-25 1.7066635459104683E-4 26-27 0.001097140850942444 28-29 7.801890495590712E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.339036027667455E-4 42-43 0.01119083667961293 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.2190453899360488E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.070463261629083E-4 84-85 2.4380907798720974E-5 86-87 0.0 88-89 0.0 90-91 0.012507405700743861 92-93 0.0019260917160989573 94-95 0.0 96-97 0.0 98-99 0.0011702835743386069 100-101 0.01867577537382027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.24074104974599 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33065992142537 46.58456259532556 2 13.418950100071699 16.167349962757648 3 4.123099742756773 7.451357517770553 4 1.805500903407019 4.350588495488988 5 0.9403270192055655 2.8322998233020997 6 0.5643657724657851 2.0398687413870733 7 0.3442817552565744 1.4517851646594326 8 0.26283933489449174 1.2666909048853232 9 0.1835222171447484 0.994996292390284 >10 0.9477023085009492 10.091877870525161 >50 0.052067817509686906 2.1455249533127234 >100 0.024899909510450242 2.700844848085967 >500 0.0013779256121517344 0.5339319929192416 >1k 1.6210889554725894E-4 0.18305542217230888 >5k 1.6210889554725894E-4 0.707514821892973 >10k+ 8.105444777362947E-5 0.4977505931247044 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10194 0.4970779482003233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8591 0.4189127577976238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5899 0.2876459502093101 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 0.10732475612996975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.8761815597441956E-5 0.0 0.0 0.0 0.0 6 4.8761815597441956E-5 0.0 0.0 0.0 0.0 7 4.8761815597441956E-5 0.0 0.0 0.0 0.0 8 4.8761815597441956E-5 0.0 0.0 0.0 0.0 9 4.8761815597441956E-5 0.0 0.0 0.0 0.0 10-11 4.8761815597441956E-5 0.0 0.0 0.0 0.0 12-13 4.8761815597441956E-5 4.8761815597441956E-5 0.0 0.0 0.0 14-15 4.8761815597441956E-5 4.8761815597441956E-5 0.0 0.0 0.0 16-17 4.8761815597441956E-5 4.8761815597441956E-5 0.0 0.0 0.0 18-19 4.8761815597441956E-5 4.8761815597441956E-5 0.0 0.0 0.0 20-21 4.8761815597441956E-5 4.8761815597441956E-5 0.0 0.0 0.0 22-23 4.8761815597441956E-5 4.8761815597441956E-5 0.0 9.752363119488391E-5 2.4380907798720978E-5 24-25 9.752363119488391E-5 4.8761815597441956E-5 0.0 1.9504726238976782E-4 4.8761815597441956E-5 26-27 9.752363119488391E-5 4.8761815597441956E-5 0.0 3.9009452477953565E-4 4.8761815597441956E-5 28-29 2.194281701884888E-4 4.8761815597441956E-5 0.0 9.264744963513971E-4 4.8761815597441956E-5 30-31 2.925708935846517E-4 4.8761815597441956E-5 0.0 0.001974853531696399 4.8761815597441956E-5 32-33 2.925708935846517E-4 4.8761815597441956E-5 0.0 0.00324266073722989 4.8761815597441956E-5 34-35 2.925708935846517E-4 4.8761815597441956E-5 0.0 0.004364182495971055 4.8761815597441956E-5 36-37 2.925708935846517E-4 4.8761815597441956E-5 0.0 0.006265893304271291 4.8761815597441956E-5 38-39 2.925708935846517E-4 4.8761815597441956E-5 0.0 0.010654456708041066 4.8761815597441956E-5 40-41 2.925708935846517E-4 4.8761815597441956E-5 0.0 0.015189305558603167 4.8761815597441956E-5 42-43 3.657136169808147E-4 4.8761815597441956E-5 0.0 0.018797679912813874 4.8761815597441956E-5 44-45 4.388563403769776E-4 4.8761815597441956E-5 0.0 0.02099196161469876 4.8761815597441956E-5 46-47 4.388563403769776E-4 4.8761815597441956E-5 0.0 0.024356526890922256 4.8761815597441956E-5 48-49 4.388563403769776E-4 4.8761815597441956E-5 0.0 0.029159565727270288 9.752363119488391E-5 50-51 4.388563403769776E-4 4.8761815597441956E-5 0.0 0.03384070002462472 9.752363119488391E-5 52-53 4.388563403769776E-4 4.8761815597441956E-5 0.0 0.04320296861933357 9.752363119488391E-5 54-55 4.8761815597441957E-4 4.8761815597441956E-5 0.0 0.05083419276033324 9.752363119488391E-5 56-57 4.8761815597441957E-4 4.8761815597441956E-5 0.0 0.06775454277264559 9.752363119488391E-5 58-59 4.8761815597441957E-4 4.8761815597441956E-5 0.0 0.09415906591866041 9.752363119488391E-5 60-61 4.8761815597441957E-4 4.8761815597441956E-5 0.0 0.11929578185914175 9.752363119488391E-5 62-63 4.8761815597441957E-4 4.8761815597441956E-5 0.0 0.1320469966378728 1.4628544679232586E-4 64-65 4.8761815597441957E-4 7.314272339616293E-5 0.0 0.15601342900401555 1.4628544679232586E-4 66-67 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.2022396301903905 1.4628544679232586E-4 68-69 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.26389894601335584 1.4628544679232586E-4 70-71 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.3277525435382061 1.4628544679232586E-4 72-73 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.3979207961829251 1.4628544679232586E-4 74-75 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.4789141718902762 1.4628544679232586E-4 76-77 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.545595954719778 1.4628544679232586E-4 78-79 4.8761815597441957E-4 9.752363119488391E-5 0.0 0.6279790421716562 1.4628544679232586E-4 80-81 5.851417871693034E-4 9.752363119488391E-5 0.0 0.7380344599750828 1.4628544679232586E-4 82-83 5.851417871693034E-4 9.752363119488391E-5 0.0 0.8380449437654361 1.4628544679232586E-4 84-85 6.339036027667455E-4 9.752363119488391E-5 0.0 0.9536835894547697 1.4628544679232586E-4 86-87 6.826654183641874E-4 9.752363119488391E-5 0.0 1.1063080722747631 1.4628544679232586E-4 88-89 6.826654183641874E-4 9.752363119488391E-5 0.0 1.306158373500879 1.4628544679232586E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5670 0.0 45.75777 1 GTATCAA 9100 0.0 35.821026 1 ATCAACG 11335 0.0 28.58538 3 TATCAAC 11395 0.0 28.434864 2 TCAACGC 11495 0.0 28.187498 4 CAACGCA 11680 0.0 27.741034 5 AACGCAG 11915 0.0 27.306192 6 GGACCGA 500 0.0 24.69835 6 ACGCAGA 13670 0.0 23.802275 7 CGCAGAG 13805 0.0 23.466286 8 GTACATG 8090 0.0 22.319803 1 GAGTACT 9105 0.0 22.041603 12-13 TACATGG 7885 0.0 21.992384 2 GCAGAGT 14950 0.0 21.51017 9 CGTACAC 245 7.510607E-9 21.330828 3 ACATGGG 7840 0.0 21.330828 3 GTACTTT 9770 0.0 20.321062 14-15 GTGTAGC 1245 0.0 20.228413 1 AGTACTT 9620 0.0 20.17035 12-13 CAGAGTA 14655 0.0 19.593267 10-11 >>END_MODULE