FastQCFastQC Report
Thu 26 May 2016
SRR937582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050807
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79960.38989529487660224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62280.30368532972629797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.20767434478232227No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26610.12975379935800882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.044.644031
GTATCAA82200.041.710481
ATCAACG101650.033.0607873
TATCAAC103250.032.6429332
TCAACGC103050.032.569494
CAACGCA105100.031.8874245
AACGCAG108000.031.0122246
ACGCAGA120800.027.5688487
CGCAGAG122200.027.0974948
GCAGAGT132050.024.5719289
GTACATG80750.024.3214381
GAGTACT75000.023.14888212-13
TACATGG83500.023.0569342
ACATGGG84050.022.678163
GTACTTT81850.021.03694214-15
AGAGTAC119550.021.01941310-11
AGTACTT80100.020.78544812-13
CAGAGTA127850.020.5465510-11
GAGTACA69450.019.445891
CATGGGA43400.019.3882124
Produced by FastQC (version 0.11.5)