##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937582_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050807 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.427183542868733 31.0 31.0 34.0 30.0 34.0 2 31.55095725731383 33.0 31.0 34.0 30.0 34.0 3 31.59615507456333 33.0 31.0 34.0 28.0 34.0 4 35.189324495186526 37.0 35.0 37.0 33.0 37.0 5 35.16859850780693 37.0 35.0 37.0 33.0 37.0 6 35.245151786589375 37.0 35.0 37.0 33.0 37.0 7 35.17116530224443 37.0 35.0 37.0 32.0 37.0 8 35.15442993904351 37.0 35.0 37.0 32.0 37.0 9 36.722713058810506 39.0 37.0 39.0 32.0 39.0 10-11 36.60132035827847 39.0 37.0 39.0 32.0 39.0 12-13 36.45543120342383 39.0 37.0 39.0 32.0 39.0 14-15 37.54311863573706 40.0 37.0 41.0 32.0 41.0 16-17 37.46841097187595 40.0 37.0 41.0 32.0 41.0 18-19 37.4051075991061 40.0 37.0 41.0 32.0 41.0 20-21 37.22093351544051 40.0 37.0 41.0 31.0 41.0 22-23 37.06079362904457 39.0 36.5 41.0 31.0 41.0 24-25 36.90497643122927 39.0 36.0 41.0 31.0 41.0 26-27 37.00910227047206 39.0 36.0 41.0 31.0 41.0 28-29 36.94865143331381 39.0 36.0 41.0 31.0 41.0 30-31 36.73435042887995 39.0 36.0 41.0 30.0 41.0 32-33 36.49067123332425 39.0 35.5 40.5 30.0 41.0 34-35 36.2194701890524 39.0 35.0 40.0 30.0 41.0 36-37 36.22034204096241 39.0 35.0 40.5 30.0 41.0 38-39 36.16163758949526 39.0 35.0 41.0 29.5 41.0 40-41 35.95775370378587 39.0 35.0 40.5 28.5 41.0 42-43 35.70363374027883 39.0 35.0 40.0 27.5 41.0 44-45 35.46619964726081 39.0 35.0 40.0 26.5 41.0 46-47 35.10400954356017 38.0 34.5 40.0 25.5 41.0 48-49 34.773569867861774 38.0 34.0 40.0 24.0 41.0 50-51 33.88338614994 37.5 33.5 39.5 23.5 40.5 52-53 33.98990421819313 38.0 33.5 39.5 23.0 40.5 54-55 34.281903660363945 38.0 34.0 40.0 23.0 41.0 56-57 33.979736513479814 38.0 33.5 40.0 21.5 41.0 58-59 33.72512211046676 38.0 33.0 40.0 20.5 41.0 60-61 33.3932754276731 37.5 33.0 40.0 19.5 41.0 62-63 33.00082138397226 37.0 32.0 40.0 18.0 41.0 64-65 32.599473768131276 36.0 32.0 39.0 14.0 41.0 66-67 32.16368385713527 36.0 31.0 39.0 11.0 40.5 68-69 31.653400100545785 35.0 31.0 39.0 8.0 40.0 70-71 31.189311573444016 35.0 30.0 38.0 7.0 40.0 72-73 30.687159981412194 35.0 30.0 37.5 2.0 39.5 74-75 30.163183322467695 34.5 29.0 37.0 2.0 39.0 76-77 29.66622456428128 34.0 29.0 36.0 2.0 39.0 78-79 29.09919997347386 34.0 28.5 36.0 2.0 38.5 80-81 28.53371916518717 34.0 27.5 35.0 2.0 37.0 82-83 27.97386516624919 33.5 26.0 35.0 2.0 37.0 84-85 27.453975922648986 33.0 26.0 35.0 2.0 36.0 86-87 26.871809243873265 33.0 25.0 35.0 2.0 36.0 88-89 26.33133761489989 32.0 24.5 35.0 2.0 35.5 90-91 25.755771996097145 32.0 22.0 34.5 2.0 35.0 92-93 25.123719345603952 32.0 19.5 34.0 2.0 35.0 94-95 24.416897104408164 31.0 14.0 34.0 2.0 35.0 96-97 23.55969040480162 31.0 2.0 34.0 2.0 35.0 98-99 22.52618773975318 31.0 2.0 34.0 2.0 35.0 100-101 19.983882686181587 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11923305802515927 1101 2 0.1837786441953142 1101 3 0.21298321619739013 1101 4 0.10505299202204554 1101 5 0.10887348298850696 1101 6 0.03677203441471022 1101 7 0.170066170190168 1101 8 0.21370134675018448 1101 9 0.3316640244526141 1101 10-11 0.13786295712735352 1101 12-13 0.1792931677140004 1101 14-15 0.2896155816186976 1101 16-17 0.23124971181663057 1101 18-19 0.24886255903810905 1101 20-21 0.366648652411925 1101 22-23 0.28547285416187407 1101 24-25 0.2718795332175148 1101 26-27 0.4387777752466988 1101 28-29 0.305894436922415 1101 30-31 0.1710463803467306 1101 32-33 0.22311366128026577 1101 34-35 0.4763217435334184 1101 36-37 0.4357559404893223 1101 38-39 0.3843555343873817 1101 40-41 0.6724963396503867 1101 42-43 0.5206617999983862 1101 44-45 0.5392447280603889 1101 46-47 0.4937611609997532 1101 48-49 0.6906700578800482 1101 50-51 0.6586707080769614 1101 52-53 0.5530739009556598 1101 54-55 0.6072565990989816 1101 56-57 0.922457214706661 1101 58-59 0.9128918175786183 1101 60-61 0.9145907633182588 1101 62-63 0.9534761642437175 1101 64-65 1.1581221433650484 1101 66-67 1.1684691840411219 1101 68-69 1.1262647415664055 1101 70-71 1.2483010806519843 1101 72-73 1.098091088266802 1101 74-75 1.082941033376425 1101 76-77 1.1533453892373444 1101 78-79 1.1182704910809385 1101 80-81 1.2693719591193222 1101 82-83 1.250889039214659 1101 84-85 1.3745422320664211 1101 86-87 1.454243094093183 1101 88-89 1.4237005883696412 1101 90-91 1.4539050515230976 1101 92-93 1.8134069417292409 1101 94-95 1.7120450527839637 1101 96-97 1.8237276881419113 1101 98-99 1.9163737757740158 1101 100-101 2.176537685237168 1102 1 0.13971391967064406 1102 2 0.22066852191418818 1102 3 0.24183598695316633 1102 4 0.13075957092546986 1102 5 0.16413851475966368 1102 6 0.23721667805451574 1102 7 0.14002303761305512 1102 8 0.13446641913466095 1102 9 0.25913400461599423 1102 10-11 0.16001370632962164 1102 12-13 0.22104592292539138 1102 14-15 0.28455798699069845 1102 16-17 0.3359157899681975 1102 18-19 0.2375541217353785 1102 20-21 0.1274491140430598 1102 22-23 0.2449179855737782 1102 24-25 0.3219738921724158 1102 26-27 0.3255316705171225 1102 28-29 0.2952842600091685 1102 30-31 0.10973676793793885 1102 32-33 0.13198604187609675 1102 34-35 0.12586790520410318 1102 36-37 0.21910648122365473 1102 38-39 0.23455868342423258 1102 40-41 0.17384173300786898 1102 42-43 0.23828917427956497 1102 44-45 0.16813458681264137 1102 46-47 0.03986211786594396 1102 48-49 0.19883168485355185 1102 50-51 0.08033865361636572 1102 52-53 0.09984621446735531 1102 54-55 0.11891045573521097 1102 56-57 0.17478866635846657 1102 58-59 0.2879712403170416 1102 60-61 0.30106459164475297 1102 62-63 0.27417796644409265 1102 64-65 0.13343203120220437 1102 66-67 0.214985963524839 1102 68-69 0.14381824909306218 1102 70-71 0.3741535994815166 1102 72-73 0.12270883863551418 1102 74-75 0.05747926823518057 1102 76-77 0.3191120346146654 1102 78-79 0.5183306070329827 1102 80-81 0.46132584300977086 1102 82-83 0.46669994692018335 1102 84-85 0.37312519847117187 1102 86-87 0.42779238443220535 1102 88-89 0.49182014654625306 1102 90-91 0.7507523038158972 1102 92-93 0.7614874575224597 1102 94-95 0.9403012613640556 1102 96-97 1.134934942628238 1102 98-99 1.2444491623323692 1102 100-101 1.595922111082409 1103 1 0.21026616374397378 1103 2 0.21998900252340192 1103 3 0.13588195629830224 1103 4 0.17634888705132568 1103 5 0.12113776684314814 1103 6 0.19047877584703343 1103 7 0.32590700370750625 1103 8 0.10588613304850014 1103 9 0.2164335565136355 1103 10-11 0.2164344563182894 1103 12-13 0.3141997751811303 1103 14-15 0.2453749319945544 1103 16-17 0.2553895044259704 1103 18-19 0.339514407692036 1103 20-21 0.3730986115467658 1103 22-23 0.32245139089272357 1103 24-25 0.24040121152305716 1103 26-27 0.0819830575623044 1103 28-29 0.17254601945849402 1103 30-31 0.07932635279570377 1103 32-33 0.18270473784640728 1103 34-35 0.21775499409285004 1103 36-37 0.26145462341197856 1103 38-39 0.35944900519021417 1103 40-41 0.2706570575259306 1103 42-43 0.3575264127516107 1103 44-45 0.15320114652617178 1103 46-47 0.2005079486360657 1103 48-49 0.3911063304835878 1103 50-51 -0.017175616695560336 1103 52-53 0.09505821318521868 1103 54-55 0.047508508885854894 1103 56-57 0.26391174233513226 1103 58-59 0.36464178956620685 1103 60-61 0.283820825901266 1103 62-63 0.32588295708704607 1103 64-65 0.4612509641746385 1103 66-67 0.4947785785777157 1103 68-69 0.6842413561519685 1103 70-71 0.6492927815651903 1103 72-73 0.4549582056585457 1103 74-75 0.594140330924187 1103 76-77 0.6084640588458647 1103 78-79 0.5555430962675167 1103 80-81 0.5150702012585491 1103 82-83 0.4530462209360735 1103 84-85 0.5173941667349027 1103 86-87 0.6564000698410304 1103 88-89 0.7217524698145503 1103 90-91 0.6983696150074472 1103 92-93 0.7505671883372678 1103 94-95 0.6947061982606186 1103 96-97 0.7687747654594403 1103 98-99 1.1709315587734501 1103 100-101 1.1538569063604989 1104 1 0.09452259597336621 1104 2 0.21207101451723176 1104 3 0.17226155447425384 1104 4 0.15560834681589597 1104 5 0.2186810588856858 1104 6 0.15601511995291872 1104 7 0.23814312066272691 1104 8 0.31395861942458936 1104 9 0.3080949634793555 1104 10-11 0.46645925816012124 1104 12-13 0.37220476206180564 1104 14-15 0.5098386819646237 1104 16-17 0.334076661690176 1104 18-19 0.4405019486150721 1104 20-21 0.5091387452030389 1104 22-23 0.404052336954706 1104 24-25 0.329272838900188 1104 26-27 0.4661882825406636 1104 28-29 0.4467135969514189 1104 30-31 0.4576442405682215 1104 32-33 0.45057681678810724 1104 34-35 0.39323133912238717 1104 36-37 0.38893769401683187 1104 38-39 0.5302141762202979 1104 40-41 0.5147222547262302 1104 42-43 0.586161122672685 1104 44-45 0.6850686268642932 1104 46-47 0.5625955966346439 1104 48-49 0.5406912631080942 1104 50-51 0.6413792441233426 1104 52-53 0.5935868866800789 1104 54-55 0.6678770856364409 1104 56-57 0.8059211447989014 1104 58-59 0.8424598560830319 1104 60-61 0.8872904752887436 1104 62-63 0.6599278467102963 1104 64-65 0.5531306701103205 1104 66-67 0.5933040021760689 1104 68-69 0.6976181012608755 1104 70-71 0.6900748135715418 1104 72-73 0.7572099135646901 1104 74-75 0.6538724667561766 1104 76-77 0.6842453382445974 1104 78-79 0.7293366577093785 1104 80-81 0.9168020967692421 1104 82-83 1.0128341609121954 1104 84-85 1.0164566143517035 1104 86-87 0.9495718448318833 1104 88-89 1.1061879670658215 1104 90-91 1.0655152351732262 1104 92-93 1.1158894455101311 1104 94-95 1.2642924693293587 1104 96-97 1.1990071843745227 1104 98-99 1.5416139002041582 1104 100-101 1.6385300095327402 1105 1 0.21984946626495017 1105 2 0.21247320899082567 1105 3 0.31131121641443826 1105 4 0.2587761170734737 1105 5 0.3201408991390693 1105 6 0.18170319719101258 1105 7 0.307995782024058 1105 8 0.30008011706832605 1105 9 0.3085411479735072 1105 10-11 0.3149635625487264 1105 12-13 0.3498574824954517 1105 14-15 0.3190948107171252 1105 16-17 0.47583066251272044 1105 18-19 0.4289437452792342 1105 20-21 0.6695239162369404 1105 22-23 0.6372972014689324 1105 24-25 0.6200056777298286 1105 26-27 0.5506243339684929 1105 28-29 0.45389968102263367 1105 30-31 0.37705557030275116 1105 32-33 0.4709703808386223 1105 34-35 0.5536989041630207 1105 36-37 0.5114153376599617 1105 38-39 0.5694002072241844 1105 40-41 0.6972647801583989 1105 42-43 0.5599074594560989 1105 44-45 0.5817280386688424 1105 46-47 0.6147611275907039 1105 48-49 0.512275804595923 1105 50-51 0.4968929742466628 1105 52-53 0.539049385885825 1105 54-55 0.5412867241990611 1105 56-57 0.6260759757061436 1105 58-59 0.8851961610469985 1105 60-61 0.7610925327648559 1105 62-63 0.9043664258186723 1105 64-65 0.7949822170577932 1105 66-67 0.7618971524410938 1105 68-69 0.7992814826410886 1105 70-71 0.9253508998282882 1105 72-73 0.9774215125460763 1105 74-75 1.0864507777209305 1105 76-77 0.8664973898902808 1105 78-79 0.9563768345632262 1105 80-81 1.0110287207865376 1105 82-83 1.0119749590028881 1105 84-85 1.1906975944772924 1105 86-87 1.0891225042283246 1105 88-89 1.0527261270121429 1105 90-91 1.273962994929061 1105 92-93 1.372553409932678 1105 94-95 1.4378324021433286 1105 96-97 1.4442599279902097 1105 98-99 1.6130404267451546 1105 100-101 1.7413919444093544 1106 1 -8.398378757306091E-4 1106 2 0.13541146011512595 1106 3 0.06254905270698785 1106 4 0.13652837092510595 1106 5 0.1976349487039144 1106 6 0.16194352731692874 1106 7 0.0772184734615351 1106 8 0.08236547456266408 1106 9 0.20354515446394572 1106 10-11 -0.11374178633221277 1106 12-13 -0.25560080787590067 1106 14-15 -0.030436826473483336 1106 16-17 0.030085560302119063 1106 18-19 -0.11506624883272565 1106 20-21 -0.010663018724990536 1106 22-23 -0.07629976214748524 1106 24-25 0.032638118615260225 1106 26-27 -0.03510443095269267 1106 28-29 0.30341982874018925 1106 30-31 0.3832087390623613 1106 32-33 0.320676213265763 1106 34-35 0.03500231940271448 1106 36-37 0.24411237656331508 1106 38-39 0.19576155535200712 1106 40-41 0.14179607513566594 1106 42-43 0.10622152531376372 1106 44-45 0.05533424822250055 1106 46-47 0.28264481360144345 1106 48-49 0.4381972418029889 1106 50-51 0.5278622929060859 1106 52-53 0.439016350562234 1106 54-55 0.2575528283015984 1106 56-57 0.4009262851683104 1106 58-59 0.3685649634955155 1106 60-61 0.16713792285469964 1106 62-63 0.39493826306941315 1106 64-65 0.533431281505834 1106 66-67 0.8186293288709692 1106 68-69 0.7499165292895817 1106 70-71 0.5444717797094327 1106 72-73 0.6992245543161211 1106 74-75 0.6723851177297746 1106 76-77 0.8532979620413279 1106 78-79 0.7835252486576714 1106 80-81 0.501301584652591 1106 82-83 0.4704048100869649 1106 84-85 0.7223531929515907 1106 86-87 0.5489277564247281 1106 88-89 0.7038225810148404 1106 90-91 0.8511518692646156 1106 92-93 0.8355888101110267 1106 94-95 0.8430080514776961 1106 96-97 0.8065645235570535 1106 98-99 0.9201834444935493 1106 100-101 0.8162342483031999 1107 1 0.4669640585719037 1107 2 0.5033152392923235 1107 3 0.6243437292528213 1107 4 0.47725460861951063 1107 5 0.5558907572337759 1107 6 0.5317552625854702 1107 7 0.5499370197165661 1107 8 0.5060040376313353 1107 9 0.6097512970724139 1107 10-11 0.699231597500912 1107 12-13 0.6926293579228044 1107 14-15 0.8809888028442927 1107 16-17 0.8230810182460786 1107 18-19 0.864248311110579 1107 20-21 0.9548078887395519 1107 22-23 0.8352194296055515 1107 24-25 0.9726390680393777 1107 26-27 0.9267567328812092 1107 28-29 0.8635318994259222 1107 30-31 0.8319007831366108 1107 32-33 0.9729474206820967 1107 34-35 0.9751691420527706 1107 36-37 0.9452870681177714 1107 38-39 0.8278780618355555 1107 40-41 0.8656974686087651 1107 42-43 0.9263258018898384 1107 44-45 0.745309185081652 1107 46-47 0.7418586297095757 1107 48-49 0.7576908785914114 1107 50-51 0.8648090498085281 1107 52-53 0.8085302868111697 1107 54-55 1.0529143946747652 1107 56-57 1.0545261343644228 1107 58-59 1.01842134929732 1107 60-61 1.112055040060831 1107 62-63 1.2068416127977954 1107 64-65 1.2600756551053323 1107 66-67 1.326057882635837 1107 68-69 1.5088841023158928 1107 70-71 1.331657810810242 1107 72-73 1.1218671880407136 1107 74-75 1.1556361998386784 1107 76-77 1.1585247074292404 1107 78-79 1.2386951803957338 1107 80-81 1.383198743804499 1107 82-83 1.6849321583315913 1107 84-85 1.7350183451395829 1107 86-87 1.766657203117461 1107 88-89 1.772791067585871 1107 90-91 1.8072439015814687 1107 92-93 1.8235882548083104 1107 94-95 1.8376342833485921 1107 96-97 2.087289226717118 1107 98-99 2.2315290075503498 1107 100-101 1.984422661520174 1108 1 0.1337390127876894 1108 2 0.1827727433860602 1108 3 0.2145249685585071 1108 4 0.24295504461648676 1108 5 0.14112903149089107 1108 6 0.08862723347470336 1108 7 0.1282771116392425 1108 8 0.20878849222594908 1108 9 0.11249889911649547 1108 10-11 0.24368478061936827 1108 12-13 0.28515122848357777 1108 14-15 0.25180042946699643 1108 16-17 0.2619318722394084 1108 18-19 0.2931831653027217 1108 20-21 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86-87 0.2666071169692863 2316 88-89 0.49896314808044906 2316 90-91 0.6276798901915548 2316 92-93 0.32973204793894695 2316 94-95 0.4810260785266216 2316 96-97 0.5522883800996468 2316 98-99 0.7573539102539684 2316 100-101 1.1163411742653224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9857.0 3 2419.0 4 2260.0 5 2528.0 6 2906.0 7 3361.0 8 4123.0 9 5144.0 10 6380.0 11 7589.0 12 9776.0 13 11704.0 14 13512.0 15 15935.0 16 18966.0 17 22849.0 18 20737.0 19 16843.0 20 16928.0 21 17621.0 22 19590.0 23 21666.0 24 24420.0 25 27621.0 26 31443.0 27 36421.0 28 42478.0 29 50525.0 30 59862.0 31 73873.0 32 90825.0 33 115313.0 34 151107.0 35 203926.0 36 277555.0 37 347635.0 38 239926.0 39 25181.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5653042028283 18.885946990059228 14.753261211430784 22.795487595681685 2 14.865261958719286 19.536881528799448 41.63220902914781 23.965647483333456 3 19.464190727254238 24.06751370886363 29.43802459381869 27.03027097006344 4 12.489426803163989 16.27592694544377 37.488082234780215 33.74656401661202 5 13.596854294516774 37.537826355453575 34.194878645179614 14.670440704850037 6 32.0365389172695 36.1089815473093 17.506822340630855 14.347657194790346 7 28.699626281375096 32.64592688717157 20.273099441205396 18.381347390247942 8 26.107315166542488 35.88982667855529 19.304466783110012 18.69839137179221 9 25.98199002899049 17.355302846325955 20.484741755935527 36.17796536874802 10-11 24.92109318101917 27.126355231792676 26.892328319797198 21.060223267390956 12-13 25.713963234356424 24.135081956319603 27.876582045001584 22.274372764322383 14-15 22.471064920850726 25.612312082311828 26.301794555135753 25.614828441701693 16-17 21.842408159037923 29.153386473647082 26.857264713411645 22.146940653903354 18-19 21.77831465985277 27.47841444989062 29.4388917320839 21.304379158172708 20-21 22.492489712512132 26.50497026050726 29.686757688167535 21.315782338813072 22-23 22.117562222147853 26.492990779198855 29.55268353967177 21.83676345898152 24-25 21.848412489999816 26.564614924242886 29.842232224101817 21.74474036165548 26-27 21.704898410352556 27.022834006119194 29.548290961055773 21.723976622472474 28-29 21.61629902059585 27.118591479582125 29.660415083248985 21.604694416573036 30-31 21.715052250371823 26.82038360587174 29.66193120188559 21.80263294187085 32-33 21.48478202500615 26.894034989652233 29.575073299238998 22.04610968610262 34-35 21.99642199542037 26.945677408416547 29.393627653485183 21.664272942677904 36-37 21.874575595534118 26.783549081429964 29.4573338557274 21.884541467308523 38-39 21.689347050166152 27.074022929665002 29.449833603374724 21.786796416794115 40-41 21.97511512904026 26.904025266843423 29.359639285135113 21.7612203189812 42-43 21.68336851526078 26.932127784408145 29.31107256107659 22.07343113925448 44-45 21.87692973610981 27.18951767345152 29.043896366819872 21.889656223618797 46-47 21.96345921038361 27.34347443326992 28.63038480290857 22.062681553437898 48-49 22.063061190037423 27.410712257087916 28.527833608871696 21.99839294400297 50-51 22.129452900955606 27.598635568681207 28.36034452306099 21.911567007302192 52-53 22.19639287175084 27.61443985032768 28.221378003720794 21.96778927420068 54-55 22.06759336499953 27.612638305512434 28.301323116119836 22.0184452133682 56-57 22.101001525930066 27.876554016165873 28.167696978814778 21.854747479089287 58-59 22.1579868760276 27.562662230137462 28.24325948127387 22.03609141256107 60-61 22.04654647541172 27.590735638662363 28.441375382852602 21.921342503073323 62-63 22.040113073687166 27.649618101558733 28.432806022455992 21.877462802298105 64-65 21.939526852939526 27.62605898830442 28.41353844350393 22.020875715252117 66-67 21.83357856754727 27.710236951199406 28.29801757138758 22.15816690986574 68-69 21.932492375969534 27.67109711550696 28.350647520562966 22.04576298796054 70-71 21.961813039270112 27.646775375689426 28.18798404411339 22.203427540927073 72-73 22.03735049775672 27.591642893897944 28.21235210350233 22.158654504843003 74-75 21.94545152113428 27.830090401196067 28.088463277368824 22.135994800300825 76-77 22.070094068540712 27.68966052039943 28.077023120857863 22.163222290201997 78-79 22.03829101680807 27.73587864269273 28.071375674608912 22.154454665890288 80-81 21.88092260799906 27.924547195248238 28.055821496258375 22.138708700494327 82-83 21.998442074726693 27.93237920584881 28.049248020238377 22.01993069918612 84-85 22.060982789451412 27.85009649051907 27.91434210754438 22.17457861248514 86-87 22.001103760845307 27.84991294452625 28.00480575518487 22.144177539443575 88-89 22.11231889720707 27.77204980229123 27.886460075332508 22.229171225169186 90-91 22.059272472745693 27.764103881666063 28.008988463291246 22.167635182296998 92-93 22.101214352436944 27.584470453199504 28.08554804598543 22.228767148378115 94-95 21.985604300442947 27.6395087078011 28.040990184217478 22.333896807538476 96-97 22.074399884727068 27.591162083020933 27.917955956948493 22.416482075303506 98-99 22.048794761239602 27.46807657859765 27.92708605310226 22.556042607060487 100-101 22.31452285643397 27.074065825435806 28.301723683208007 22.309687634922216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2421.0 1 1404.0 2 418.5 3 472.0 4 387.5 5 341.5 6 588.5 7 1268.5 8 2082.0 9 2439.5 10 2249.0 11 2001.5 12 2062.5 13 2181.5 14 2359.5 15 2603.0 16 2772.5 17 2947.5 18 3191.0 19 3469.0 20 3929.0 21 4655.5 22 5662.5 23 6952.0 24 8563.0 25 10783.0 26 13513.5 27 16605.5 28 19465.0 29 22457.0 30 26182.0 31 30010.0 32 34076.0 33 38020.0 34 41768.5 35 45584.0 36 50139.0 37 54330.0 38 57629.0 39 60551.0 40 63416.0 41 66919.0 42 69481.5 43 72007.0 44 75249.5 45 77538.5 46 77950.5 47 78871.5 48 81325.5 49 80184.0 50 76741.0 51 73313.0 52 68874.5 53 66038.0 54 63897.0 55 59243.5 56 53318.0 57 47566.0 58 42612.0 59 37279.5 60 30600.5 61 24842.0 62 19803.0 63 14654.0 64 10695.0 65 8239.0 66 6131.0 67 4539.5 68 3318.0 69 2395.0 70 1737.5 71 1197.5 72 889.5 73 692.0 74 531.0 75 418.5 76 313.5 77 196.0 78 138.0 79 94.0 80 55.0 81 41.0 82 29.5 83 21.0 84 18.0 85 16.5 86 15.0 87 9.0 88 2.5 89 3.5 90 5.0 91 3.5 92 5.5 93 4.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3119747494522888 2 0.2702350830672998 3 0.2640423989190597 4 0.270186321774794 5 0.2733558057876729 6 0.2058701769596066 7 0.198946073423779 8 0.19660553138349926 9 0.2084057641699097 10-11 0.20711358991850523 12-13 0.2032370671642919 14-15 0.2047730478782255 16-17 0.1901202794802241 18-19 0.19550840230211813 20-21 0.18439082761078932 22-23 0.19346042801687335 24-25 0.19355795060188502 26-27 0.19355795060188502 28-29 0.20506561563326045 30-31 0.20104280900152963 32-33 0.20628464794590615 34-35 0.2016523251578525 36-37 0.18631689866476953 38-39 0.1750530400959232 40-41 0.17256621417812595 42-43 0.18665822771231033 44-45 0.1899252343102008 46-47 0.18963266655516586 48-49 0.20516313821827212 50-51 0.19960435087260772 52-53 0.21796297750105204 54-55 0.19185130536418105 56-57 0.17217612383807934 58-59 0.1736389626132542 60-61 0.16325280730951278 62-63 0.1691773043489709 64-65 0.16832398173011892 66-67 0.1679582720363252 68-69 0.1705426205391341 70-71 0.17249307223936725 72-73 0.16927482693398258 74-75 0.18361064693069606 76-77 0.17697911114990345 78-79 0.18017297580903519 80-81 0.17393153036828918 82-83 0.15662127152872016 84-85 0.1558654714948798 86-87 0.15905933615401158 88-89 0.16339909118703028 90-91 0.17144470445049192 92-93 0.16673923972367952 94-95 0.16530078159475758 96-97 0.17027443343035206 98-99 0.17951469836020648 100-101 0.16242386533691372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.25989364709641 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53011860107722 50.31066844445109 2 12.155195876487914 15.378727968125006 3 3.8026279115493287 7.216615117922727 4 1.5940941163625075 4.033688970582175 5 0.8143225922552215 2.5756980290246587 6 0.5029521409365679 1.9090019367136044 7 0.3166574235619462 1.4022200455964595 8 0.22401052911225425 1.1336705797976798 9 0.16631384919736394 0.9468896771038026 >10 0.8241690953018721 9.268960590861912 >50 0.04876434876262248 2.086578780750277 >100 0.01961581707133792 2.319335713142633 >500 6.946189942478554E-4 0.2562767310094464 >1k 3.087195529990227E-4 0.4671960447759997 >5k 1.5435977649951135E-4 0.6944713701425248 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7996 0.38989529487660224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6228 0.30368532972629797 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.20767434478232227 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2661 0.12975379935800882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.8761292505828196E-5 0.0 0.0 0.0 0.0 3 4.8761292505828196E-5 0.0 0.0 0.0 0.0 4 4.8761292505828196E-5 0.0 0.0 0.0 0.0 5 4.8761292505828196E-5 0.0 0.0 0.0 0.0 6 4.8761292505828196E-5 0.0 0.0 0.0 0.0 7 4.8761292505828196E-5 0.0 0.0 0.0 0.0 8 4.8761292505828196E-5 0.0 0.0 0.0 0.0 9 4.8761292505828196E-5 0.0 0.0 0.0 0.0 10-11 4.8761292505828196E-5 0.0 0.0 0.0 0.0 12-13 9.752258501165639E-5 0.0 0.0 0.0 0.0 14-15 9.752258501165639E-5 0.0 0.0 0.0 0.0 16-17 9.752258501165639E-5 0.0 0.0 0.0 0.0 18-19 9.752258501165639E-5 0.0 0.0 0.0 0.0 20-21 9.752258501165639E-5 0.0 0.0 2.4380646252914098E-5 0.0 22-23 9.752258501165639E-5 0.0 0.0 9.752258501165639E-5 2.4380646252914098E-5 24-25 9.752258501165639E-5 0.0 0.0 2.4380646252914097E-4 4.8761292505828196E-5 26-27 9.752258501165639E-5 0.0 0.0 3.1694840128788324E-4 4.8761292505828196E-5 28-29 1.706645237703987E-4 0.0 0.0 8.533226188519934E-4 4.8761292505828196E-5 30-31 1.9504517002331278E-4 0.0 0.0 0.0025843485028088945 4.8761292505828196E-5 32-33 1.9504517002331278E-4 0.0 0.0 0.0049736518355944755 4.8761292505828196E-5 34-35 1.9504517002331278E-4 0.0 0.0 0.006826580950815947 4.8761292505828196E-5 36-37 2.4380646252914097E-4 0.0 0.0 0.009289026222360271 4.8761292505828196E-5 38-39 2.4380646252914097E-4 0.0 0.0 0.01326307156158527 4.8761292505828196E-5 40-41 2.4380646252914097E-4 2.4380646252914098E-5 0.0 0.01772472982586855 4.8761292505828196E-5 42-43 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.022381433260175138 4.8761292505828196E-5 44-45 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.024380646252914098 4.8761292505828196E-5 46-47 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.02725756251075796 4.8761292505828196E-5 48-49 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.03125598849623587 4.8761292505828196E-5 50-51 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.03603459516180703 4.8761292505828196E-5 52-53 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.04703026662187129 4.8761292505828196E-5 54-55 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.05505149923908003 4.8761292505828196E-5 56-57 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.07375145491506514 4.8761292505828196E-5 58-59 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.1006433077320294 4.8761292505828196E-5 60-61 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.1257066120800251 4.8761292505828196E-5 62-63 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.1394329159204157 4.8761292505828196E-5 64-65 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.16320404601700694 4.8761292505828196E-5 66-67 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.21130706107400649 4.8761292505828196E-5 68-69 2.4380646252914097E-4 4.8761292505828196E-5 0.0 0.2796460125209247 4.8761292505828196E-5 70-71 2.6818710878205506E-4 4.8761292505828196E-5 0.0 0.3449373831862287 4.8761292505828196E-5 72-73 2.9256775503496915E-4 4.8761292505828196E-5 0.0 0.4198347284751808 4.8761292505828196E-5 74-75 2.9256775503496915E-4 4.8761292505828196E-5 0.0 0.5038748161089757 4.8761292505828196E-5 76-77 2.9256775503496915E-4 4.8761292505828196E-5 0.0 0.5703120771481665 4.8761292505828196E-5 78-79 2.9256775503496915E-4 4.8761292505828196E-5 0.0 0.6546203518907435 4.8761292505828196E-5 80-81 3.4132904754079733E-4 4.8761292505828196E-5 0.0 0.7687217763543814 4.8761292505828196E-5 82-83 3.4132904754079733E-4 4.8761292505828196E-5 0.0 0.8724858068067839 4.8761292505828196E-5 84-85 3.4132904754079733E-4 4.8761292505828196E-5 0.0 0.9928530573574208 4.8761292505828196E-5 86-87 3.4132904754079733E-4 4.8761292505828196E-5 0.0 1.1484259610972656 4.8761292505828196E-5 88-89 3.4132904754079733E-4 4.8761292505828196E-5 0.0 1.3541986154718606 4.8761292505828196E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4740 0.0 44.64403 1 GTATCAA 8220 0.0 41.71048 1 ATCAACG 10165 0.0 33.060787 3 TATCAAC 10325 0.0 32.642933 2 TCAACGC 10305 0.0 32.56949 4 CAACGCA 10510 0.0 31.887424 5 AACGCAG 10800 0.0 31.012224 6 ACGCAGA 12080 0.0 27.568848 7 CGCAGAG 12220 0.0 27.097494 8 GCAGAGT 13205 0.0 24.571928 9 GTACATG 8075 0.0 24.321438 1 GAGTACT 7500 0.0 23.148882 12-13 TACATGG 8350 0.0 23.056934 2 ACATGGG 8405 0.0 22.67816 3 GTACTTT 8185 0.0 21.036942 14-15 AGAGTAC 11955 0.0 21.019413 10-11 AGTACTT 8010 0.0 20.785448 12-13 CAGAGTA 12785 0.0 20.54655 10-11 GAGTACA 6945 0.0 19.44589 1 CATGGGA 4340 0.0 19.388212 4 >>END_MODULE