FastQCFastQC Report
Thu 26 May 2016
SRR937582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050807
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102060.49765775131448253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88300.4305622128264629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.29290908408251No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.11195592759338154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.045.054541
GTATCAA92950.037.5260161
ATCAACG117850.029.4274083
TCAACGC119200.029.133984
GGACCGA4600.028.9117376
TATCAAC119800.028.9087582
CAACGCA121150.028.5859225
AACGCAG124900.027.7609566
AGGACCG5600.024.6013035
ACGCAGA142050.024.3113757
CGCAGAG143650.023.9744538
GTACATG78350.022.6839371
GAGTACT95400.022.65579812-13
TACATGG78050.022.0340542
GCAGAGT154550.021.8532969
GTACTTT100200.021.35507414-15
AGTACTT99350.021.20518712-13
ACATGGG78450.021.1950173
ACCGAGT5850.021.1122178
AGAGTAC142100.019.73977510-11
Produced by FastQC (version 0.11.5)